Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_B15
(447 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precurs... 142 3e-34
sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precurs... 142 4e-34
sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precurs... 140 1e-33
sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precurs... 140 2e-33
sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor... 137 1e-32
sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl este... 136 2e-32
sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl este... 134 1e-31
sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3) 126 2e-29
sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester ... 124 1e-28
sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1) 123 2e-28
>sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 395
Score = 142 bits (358), Expect = 3e-34
Identities = 72/133 (54%), Positives = 89/133 (66%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP+G K E K RPV LQHGL+ SA W N P SLAFILAD G+
Sbjct: 55 GYILGVYRIPYGKKNSENIGK----RPVAYLQHGLIASATNWITNLPNNSLAFILADAGY 110
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL NSRGNT+S K+V + +FW FS+DEMA +DLPA +D+ ++ +G K+ Y+G
Sbjct: 111 DVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIDFIVQKTGQ-EKIHYVG 169
Query: 362 HSQGTEIAFAQFS 400
HSQGT I F FS
Sbjct: 170 HSQGTTIGFIAFS 182
>sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 142 bits (357), Expect = 4e-34
Identities = 72/133 (54%), Positives = 89/133 (66%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP+G K E + RPV LQHGLL SA W N P SLAFILAD G+
Sbjct: 56 GYILGIDRIPYGRKNSENIGR----RPVAFLQHGLLASATNWISNLPNNSLAFILADAGY 111
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL NSRGNT++ +++ + +FW FS+DEMA +DLPA +D+ LK +G KL Y+G
Sbjct: 112 DVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFILKKTGQ-DKLHYVG 170
Query: 362 HSQGTEIAFAQFS 400
HSQGT I F FS
Sbjct: 171 HSQGTTIGFIAFS 183
>sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 140 bits (354), Expect = 1e-33
Identities = 71/133 (53%), Positives = 89/133 (66%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP+G K T RPVV LQHGLL SA W N P SLAFILAD G+
Sbjct: 56 GYILEVNRIPYGKKNSGN----TGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGY 111
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL NSRGNT++ +++ + +FW FS+DEMA +DLPA +D+ +K +G +L Y+G
Sbjct: 112 DVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQ-KQLHYVG 170
Query: 362 HSQGTEIAFAQFS 400
HSQGT I F FS
Sbjct: 171 HSQGTTIGFIAFS 183
>sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Pregastric esterase) (PGE)
Length = 397
Score = 140 bits (352), Expect = 2e-33
Identities = 69/133 (51%), Positives = 88/133 (66%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP G + RPVV LQHGLL SA W N P+ SL F+LAD G+
Sbjct: 55 GYILQVYRIPHGKNNANHLGQ----RPVVFLQHGLLGSATNWISNLPKNSLGFLLADAGY 110
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL NSRGNT++ +H+ + P+FW FS+DEMA +DLP+ +D+ L+ +G KL Y+G
Sbjct: 111 DVWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTIDFILRRTGQ-KKLHYVG 169
Query: 362 HSQGTEIAFAQFS 400
HSQGT I F FS
Sbjct: 170 HSQGTTIGFIAFS 182
>sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Lingual lipase)
Length = 395
Score = 137 bits (345), Expect = 1e-32
Identities = 70/133 (52%), Positives = 88/133 (66%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP G E K RPVV LQHGL+ SA W N P SLAF+LAD G+
Sbjct: 55 GYILGVYRIPHGKNNSENIGK----RPVVYLQHGLIASATNWIANLPNNSLAFMLADAGY 110
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL NSRGNT+S K+V + +FW FS+DEMA +DLPA +++ ++ +G K+ Y+G
Sbjct: 111 DVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATINFIVQKTGQ-EKIHYVG 169
Query: 362 HSQGTEIAFAQFS 400
HSQGT I F FS
Sbjct: 170 HSQGTTIGFIAFS 182
>sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 399
Score = 136 bits (342), Expect = 2e-32
Identities = 68/134 (50%), Positives = 86/134 (64%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL + RIP G K + +PVV LQHGLL + W N SL FILAD GF
Sbjct: 58 GYILCLNRIPHGRKNHSDKGP----KPVVFLQHGLLADSSNWVTNLANSSLGFILADAGF 113
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVW+ NSRGNT+S KH +L+ +FW FS+DEMA +DLPA +++ L +G ++ Y+G
Sbjct: 114 DVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQ-EQVYYVG 172
Query: 362 HSQGTEIAFAQFSQ 403
HSQGT I F FSQ
Sbjct: 173 HSQGTTIGFIAFSQ 186
>sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 134 bits (336), Expect = 1e-31
Identities = 69/134 (51%), Positives = 86/134 (64%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYILS+ RIP G K + RPVV LQHGLL + W N SL F+LAD GF
Sbjct: 56 GYILSIHRIPRGWKNHFGKGP----RPVVYLQHGLLADSSNWVTNIDNSSLGFLLADRGF 111
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVW+ NSRGNT+S KH +L+ +FW FS+DEMA +DLPA ++Y L +G ++ Y+G
Sbjct: 112 DVWMGNSRGNTWSLKHKTLSVSQDEFWAFSFDEMAKYDLPASINYILNKTGQ-EQIYYVG 170
Query: 362 HSQGTEIAFAQFSQ 403
HSQG I F FSQ
Sbjct: 171 HSQGCTIGFIAFSQ 184
>sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3)
Length = 394
Score = 126 bits (317), Expect = 2e-29
Identities = 60/133 (45%), Positives = 84/133 (63%)
Frame = +2
Query: 5 YILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGFD 184
YIL+M RIP+ KT E + +RPV L HG+L S+ W + PE+SLA++LAD G+D
Sbjct: 50 YILTMHRIPYSPKTGE-----SSNRPVAFLMHGMLSSSSDWVLMGPERSLAYMLADAGYD 104
Query: 185 VWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIGH 364
VW+ N+RGNTYS H FW FSW+E+ +D+PA +DY L +G ++ Y+GH
Sbjct: 105 VWMGNARGNTYSKAHKYWPTYWQIFWNFSWNEIGMYDVPAMIDYVLAKTGQ-QQVQYVGH 163
Query: 365 SQGTEIAFAQFSQ 403
SQGT + S+
Sbjct: 164 SQGTTVYLVMVSE 176
>sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 124 bits (310), Expect = 1e-28
Identities = 66/136 (48%), Positives = 84/136 (61%), Gaps = 2/136 (1%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQ--HGLLDSAHTWAINSPEQSLAFILADH 175
GYIL + RIP G K + + +PVV LQ HG L + W N SL FILAD
Sbjct: 54 GYILGVHRIPHGRKNQFDKGP----KPVVYLQWRHGFLADSSNWVTNIDNNSLGFILADA 109
Query: 176 GFDVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTY 355
GFDVW+ NSRGNT+S KH +L+ ++W FS+DEMA +DLPA ++Y L +G +L
Sbjct: 110 GFDVWMGNSRGNTWSRKHKTLSVSQDEYWAFSFDEMAKYDLPASINYILNKTGQ-EQLYN 168
Query: 356 IGHSQGTEIAFAQFSQ 403
+GHSQG I F FSQ
Sbjct: 169 VGHSQGCTIGFIAFSQ 184
>sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1)
Length = 439
Score = 123 bits (309), Expect = 2e-28
Identities = 62/133 (46%), Positives = 81/133 (60%)
Frame = +2
Query: 2 GYILSMQRIPFGSKTRERQTKMTQSRPVVLLQHGLLDSAHTWAINSPEQSLAFILADHGF 181
GYIL+M RI Q P LLQHGL+DS+ + + P SLA++LADH +
Sbjct: 89 GYILTMHRI------------RKQGAPPFLLQHGLVDSSAGFVVMGPNVSLAYLLADHNY 136
Query: 182 DVWLANSRGNTYSNKHVSLNERDPKFWEFSWDEMASFDLPACVDYALKISGNATKLTYIG 361
DVWL N+RGN YS H +L+ + KFW+FSW E+ +DLPA +D+ LK++G KL Y G
Sbjct: 137 DVWLGNARGNRYSRNHTTLDPDESKFWDFSWHEIGMYDLPAMIDHVLKVTG-FPKLHYAG 195
Query: 362 HSQGTEIAFAQFS 400
HSQG F S
Sbjct: 196 HSQGCTSFFVMCS 208
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,168,350
Number of Sequences: 369166
Number of extensions: 1207177
Number of successful extensions: 3201
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3070
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3179
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2394311040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)