Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_O21
(461 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P10735|RT12_DROME 40S ribosomal protein S12, mitochondri... 141 6e-34
sp|O35680|RT12_MOUSE 28S ribosomal protein S12, mitochondri... 120 1e-27
sp|O15235|RT12_HUMAN 28S ribosomal protein S12, mitochondri... 109 3e-24
sp|Q5RB32|RT12_PONPY 28S ribosomal protein S12, mitochondri... 108 6e-24
sp|O70089|RS12_AQUAE 30S ribosomal protein S12 105 5e-23
sp|Q6MER6|RS12_PARUW 30S ribosomal protein S12 103 2e-22
sp|Q5LMR2|RS12_SILPO 30S ribosomal protein S12 103 2e-22
sp|P66371|RS12_CHLMU 30S ribosomal protein S12 >gi|54042115... 103 2e-22
sp|Q9Z800|RS12_CHLPN 30S ribosomal protein S12 103 2e-22
sp|Q9X1J3|RS12_THEMA 30S ribosomal protein S12 103 2e-22
>sp|P10735|RT12_DROME 40S ribosomal protein S12, mitochondrial precursor (MT-RPS12)
(Technical knockout locus protein)
Length = 140
Score = 141 bits (356), Expect = 6e-34
Identities = 77/135 (57%), Positives = 92/135 (68%), Gaps = 7/135 (5%)
Frame = +1
Query: 40 MNFLTKSFGVFVNLSSQVF-------VRSFASIEYMAMHGPFRKKRAPNFHMGHVPFMKG 198
MNFL +SFG+ L+SQ VR AS++ M GP K R P + PF KG
Sbjct: 1 MNFLRQSFGITKQLASQAIQCSYETAVRGMASLQQMHRSGPHIKTRPPRQPLDGKPFAKG 60
Query: 199 IVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQEHNEVLIKGGRVQDLIG 378
+VLKTLI+KPKKPNSANR+CV VRLS G E++AYIPG G+NLQEHN VL + GR+QD+ G
Sbjct: 61 VVLKTLIKKPKKPNSANRKCVLVRLSTGKEMVAYIPGIGHNLQEHNIVLCRVGRLQDVPG 120
Query: 379 VKHKVVRGVLDCAAV 423
VK K VRGV D A V
Sbjct: 121 VKLKAVRGVYDLAHV 135
>sp|O35680|RT12_MOUSE 28S ribosomal protein S12, mitochondrial precursor (S12mt)
(MRP-S12) (MT-RPS12)
Length = 139
Score = 120 bits (302), Expect = 1e-27
Identities = 67/130 (51%), Positives = 87/130 (66%), Gaps = 4/130 (3%)
Frame = +1
Query: 49 LTKSFGVFVNLSSQVFV-RSFASIEYMAMHGPFRKKRAPNFHMGHV---PFMKGIVLKTL 216
LT S + L+ Q++ RS A++ M GP ++ P +G P +KG+VL+T
Sbjct: 10 LTTSLSRGLALAPQLWAARSMATLNQMHRLGP---RKEPPKRLGPTEGRPQLKGVVLRTF 66
Query: 217 IRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQEHNEVLIKGGRVQDLIGVKHKVV 396
IRKPKKPNSANR+C +VRLS G E + +IPGEG+ LQEH+ VL++GGR QDL GVK KVV
Sbjct: 67 IRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVV 126
Query: 397 RGVLDCAAVQ 426
RG DC VQ
Sbjct: 127 RGKYDCGHVQ 136
>sp|O15235|RT12_HUMAN 28S ribosomal protein S12, mitochondrial precursor (S12mt)
(MRP-S12) (MT-RPS12)
Length = 138
Score = 109 bits (273), Expect = 3e-24
Identities = 61/111 (54%), Positives = 73/111 (65%), Gaps = 3/111 (2%)
Frame = +1
Query: 103 SFASIEYMAMHGPFRKKRAPNFHMGHV---PFMKGIVLKTLIRKPKKPNSANRRCVKVRL 273
S A++ M GP KR P +G P +KG+VL T RKPKKPNSANR+C +VRL
Sbjct: 29 SMATLNQMHRLGP--PKRPPR-KLGPTEGRPQLKGVVLCTFTRKPKKPNSANRKCCRVRL 85
Query: 274 SNGLEVIAYIPGEGNNLQEHNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
S G E + +IPGEG+ LQEH VL++GGR QDL GVK VVRG DC VQ
Sbjct: 86 STGREAVCFIPGEGHTLQEHQIVLVEGGRTQDLPGVKLTVVRGKYDCGHVQ 136
>sp|Q5RB32|RT12_PONPY 28S ribosomal protein S12, mitochondrial precursor (S12mt)
(MRP-S12) (MT-RPS12)
Length = 139
Score = 108 bits (270), Expect = 6e-24
Identities = 52/81 (64%), Positives = 61/81 (75%)
Frame = +1
Query: 184 PFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQEHNEVLIKGGRV 363
P +KG+VL T RKPKKPNSANR+C +VRLS G E + +IPGEG+ LQEH VL++GGR
Sbjct: 57 PQLKGVVLCTFTRKPKKPNSANRKCCRVRLSTGQEAVCFIPGEGHTLQEHQIVLVEGGRT 116
Query: 364 QDLIGVKHKVVRGVLDCAAVQ 426
QDL GVK VVRG DC VQ
Sbjct: 117 QDLPGVKLTVVRGKYDCGHVQ 137
>sp|O70089|RS12_AQUAE 30S ribosomal protein S12
Length = 128
Score = 105 bits (262), Expect = 5e-23
Identities = 50/94 (53%), Positives = 70/94 (74%)
Frame = +1
Query: 145 RKKRAPNFHMGHVPFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNL 324
RKK++ + P +G+ ++ PKKPNSA R+ +VRLSNG+EV AYIPGEG+NL
Sbjct: 15 RKKKSKAPALQGCPQKRGVCVRVYTVTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNL 74
Query: 325 QEHNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
QEH+ VL++GGRV+DL GV++K++RG LD A V+
Sbjct: 75 QEHSIVLVRGGRVKDLPGVRYKIIRGALDAAGVE 108
>sp|Q6MER6|RS12_PARUW 30S ribosomal protein S12
Length = 126
Score = 103 bits (257), Expect = 2e-22
Identities = 51/94 (54%), Positives = 70/94 (74%)
Frame = +1
Query: 145 RKKRAPNFHMGHVPFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNL 324
+K+R+ + + P +G+ L+ + PKKPNSA R+ VRLS G EVIAYI GEG+NL
Sbjct: 15 KKRRSKSPALQKCPQRRGVCLQVKTKTPKKPNSALRKVAWVRLSTGQEVIAYIGGEGHNL 74
Query: 325 QEHNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
QEH+ VL++GGRV+DL GV++ +VRG LDCAAV+
Sbjct: 75 QEHSIVLVRGGRVKDLPGVRYHIVRGALDCAAVK 108
>sp|Q5LMR2|RS12_SILPO 30S ribosomal protein S12
Length = 123
Score = 103 bits (256), Expect = 2e-22
Identities = 49/92 (53%), Positives = 67/92 (72%)
Frame = +1
Query: 151 KRAPNFHMGHVPFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQE 330
KR+ + H+ P +G+ + PKKPNSA R+ KVRL+NG EVI+YIPGE +NLQE
Sbjct: 17 KRSKSQHLEQCPQKRGVCTRVYTTTPKKPNSAMRKVAKVRLTNGYEVISYIPGESHNLQE 76
Query: 331 HNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
H+ VLI+GGRV+DL GV++ ++RGVLD V+
Sbjct: 77 HSVVLIRGGRVKDLPGVRYHILRGVLDTQGVK 108
>sp|P66371|RS12_CHLMU 30S ribosomal protein S12
sp|P66370|RS12_CHLTR 30S ribosomal protein S12
Length = 123
Score = 103 bits (256), Expect = 2e-22
Identities = 52/93 (55%), Positives = 69/93 (74%)
Frame = +1
Query: 148 KKRAPNFHMGHVPFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQ 327
+K++P P +G+ L+ + PKKPNSA R+ VRLSNG EVIAYI GEG+NLQ
Sbjct: 18 RKKSPALQKS--PQKRGVCLQVKTKTPKKPNSALRKVAWVRLSNGQEVIAYIGGEGHNLQ 75
Query: 328 EHNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
EH+ VL++GGRV+DL GV++ +VRG LDCAAV+
Sbjct: 76 EHSIVLVQGGRVKDLPGVRYHIVRGALDCAAVK 108
>sp|Q9Z800|RS12_CHLPN 30S ribosomal protein S12
Length = 123
Score = 103 bits (256), Expect = 2e-22
Identities = 51/81 (62%), Positives = 64/81 (79%)
Frame = +1
Query: 184 PFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNLQEHNEVLIKGGRV 363
P +G+ L+ + PKKPNSA R+ VRLSNG EVIAYI GEG+NLQEH+ VLI+GGRV
Sbjct: 28 PQKRGVCLQVKTKTPKKPNSALRKVAWVRLSNGQEVIAYIGGEGHNLQEHSIVLIQGGRV 87
Query: 364 QDLIGVKHKVVRGVLDCAAVQ 426
+DL GV++ +VRG LDCAAV+
Sbjct: 88 KDLPGVRYHIVRGTLDCAAVK 108
>sp|Q9X1J3|RS12_THEMA 30S ribosomal protein S12
Length = 127
Score = 103 bits (256), Expect = 2e-22
Identities = 50/94 (53%), Positives = 68/94 (72%)
Frame = +1
Query: 145 RKKRAPNFHMGHVPFMKGIVLKTLIRKPKKPNSANRRCVKVRLSNGLEVIAYIPGEGNNL 324
+K +AP P +G+ +K PKKPNSA R+ +VRLSNG+EV AYIPG G+NL
Sbjct: 17 KKSKAPALQGN--PQKRGVCIKVSTMTPKKPNSALRKIARVRLSNGIEVTAYIPGIGHNL 74
Query: 325 QEHNEVLIKGGRVQDLIGVKHKVVRGVLDCAAVQ 426
QEH+ VL++GGRV+DL GV++K++RG LD A V+
Sbjct: 75 QEHSVVLVRGGRVKDLPGVRYKIIRGALDAAGVE 108
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,364,998
Number of Sequences: 369166
Number of extensions: 790401
Number of successful extensions: 1829
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1798
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2584230740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)