Planarian EST Database


Dr_sW_013_K09-1

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_013_K09-1
         (572 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P19199|POL_COYMV  Putative polyprotein [Contains: Coat pr...    38   0.016
sp|P80303|NUCB2_HUMAN  Nucleobindin 2 precursor (DNA-binding...    35   0.10 
sp|Q9QWF0|CAF1A_MOUSE  Chromatin assembly factor 1 subunit A...    35   0.10 
sp|P34623|YO87_CAEEL  Hypothetical protein ZK1236.7 in chrom...    35   0.13 
sp|Q8N4C8|MINK1_HUMAN  Misshapen-like kinase 1 (Mitogen-acti...    35   0.17 
sp|Q9JM52|MINK1_MOUSE  Misshapen-like kinase 1 (Mitogen-acti...    35   0.17 
sp|P16947|M49_STRP9  M protein, serotype 49 precursor              34   0.23 
sp|Q5P9F6|SYT_ANAMM  Threonyl-tRNA synthetase (Threonine--tR...    34   0.23 
sp|Q97WH0|RAD50_SULSO  DNA double-strand break repair rad50 ...    34   0.30 
sp|P26805|GAG_MLVFP  Gag polyprotein (Core polyprotein) [Con...    34   0.30 
>sp|P19199|POL_COYMV Putative polyprotein [Contains: Coat protein; Protease ; Reverse
           transcriptase ; Ribonuclease H ]
          Length = 1886

 Score = 38.1 bits (87), Expect = 0.016
 Identities = 30/124 (24%), Positives = 61/124 (49%), Gaps = 2/124 (1%)
 Frame = -1

Query: 536 SESRSPVIHRKHR--DHPKSYALPEKKESEKLVENGXXXXXXXXXXKGSPELTHNINPKD 363
           SE+ +P I       D    +A  ++ +S++ +++G          + SPE   N +   
Sbjct: 395 SETSTPTIDESEAIIDEFLEHAYEQRCDSDESLQSGDPRKYEYPTPQSSPEHLDNESRSR 454

Query: 362 FSPAESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIKE 183
            S A S  M+D DV E ++      ++K+ ++  ++ +  +A+EE +I +   E K+ +E
Sbjct: 455 SSSASSTSMQD-DVEEIVRLMKEMRMKKQKKKKAQQALSSQAQEEPIIEENIEENKQAQE 513

Query: 182 EVTR 171
           E T+
Sbjct: 514 EPTQ 517
>sp|P80303|NUCB2_HUMAN Nucleobindin 2 precursor (DNA-binding protein NEFA) (Gastric cancer
           antigen Zg4)
          Length = 420

 Score = 35.4 bits (80), Expect = 0.10
 Identities = 30/124 (24%), Positives = 53/124 (42%)
 Frame = -1

Query: 548 KMPRSESRSPVIHRKHRDHPKSYALPEKKESEKLVENGXXXXXXXXXXKGSPELTHNINP 369
           K    ES+   + +KH +HPK      K + +++ E                  T  ++P
Sbjct: 199 KRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEE-----------------TDGLDP 241

Query: 368 KDFSPAESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRI 189
            DF P    ++ D +    L +Q+L  +  K  E   +  DPK EE+ ++  +  E  R+
Sbjct: 242 NDFDPKTFFKLHDVNSDGFLDEQELEALFTKELE---KVYDPKNEEDDMVEME-EERLRM 297

Query: 188 KEEV 177
           +E V
Sbjct: 298 REHV 301
>sp|Q9QWF0|CAF1A_MOUSE Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin
           assembly factor I p150 subunit) (CAF-I 150 kDa subunit)
           (CAF-Ip150)
          Length = 911

 Score = 35.4 bits (80), Expect = 0.10
 Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 13/82 (15%)
 Frame = -1

Query: 368 KDFSPAESGEMRDSDVLEDLKDQDLNE---------IEKKVQETRKRNVDPKA-EEERLI 219
           K+    E  E R+ D  E  + Q L E         +E K++E RK+  + +  EEE+ +
Sbjct: 351 KELKEKERREKREKDEKEKAEKQRLKEEKRKERQEALEAKLEEKRKKEEEKRLREEEKRL 410

Query: 218 RQKWAEEKRIKEE---VTRRFE 162
           R+   EEKRIK E   +TR F+
Sbjct: 411 RE---EEKRIKAEKAEITRFFQ 429

 Score = 30.4 bits (67), Expect = 3.3
 Identities = 18/52 (34%), Positives = 29/52 (55%)
 Frame = -1

Query: 335 RDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIKEE 180
           RD +   + K++   EI +  +E RK     K EEE+ +++K   EKR K+E
Sbjct: 320 RDREQQREEKEKLREEIRRAKEEARK-----KKEEEKELKEKERREKREKDE 366
>sp|P34623|YO87_CAEEL Hypothetical protein ZK1236.7 in chromosome III
          Length = 324

 Score = 35.0 bits (79), Expect = 0.13
 Identities = 20/59 (33%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
 Frame = -1

Query: 350 ESGEMRDSDVLE--DLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIKEE 180
           E  +MR+ +V E  + K ++    +K+ +E  K   D KAEEERL +++  +E++  EE
Sbjct: 144 EKRQMREYEVREREERKRREEEREKKRDEERAKEEADEKAEEERLRKEREEKERKEHEE 202
>sp|Q8N4C8|MINK1_HUMAN Misshapen-like kinase 1 (Mitogen-activated protein kinase kinase
           kinase kinase 6) (MAPK/ERK kinase kinase kinase 6) (MEK
           kinase kinase 6) (MEKKK 6) (Misshapen/NIK-related
           kinase) (GCK family kinase MiNK)
          Length = 1332

 Score = 34.7 bits (78), Expect = 0.17
 Identities = 26/135 (19%), Positives = 56/135 (41%)
 Frame = -1

Query: 548 KMPRSESRSPVIHRKHRDHPKSYALPEKKESEKLVENGXXXXXXXXXXKGSPELTHNINP 369
           ++ + + R P  H KH  H +   + E+KE  + VE                        
Sbjct: 374 QLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEE-----------------QQRRER 416

Query: 368 KDFSPAESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRI 189
           +     E  + R  + ++ L+ ++     ++ QE +++ ++ + + ERL RQ   E   +
Sbjct: 417 EQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYL 476

Query: 188 KEEVTRRFEIYIQKQ 144
           K    ++ +  +QKQ
Sbjct: 477 KSLQQQQQQQQLQKQ 491
>sp|Q9JM52|MINK1_MOUSE Misshapen-like kinase 1 (Mitogen-activated protein kinase kinase
           kinase kinase 6) (MAPK/ERK kinase kinase kinase 6) (MEK
           kinase kinase 6) (MEKKK 6) (Misshapen/NIK-related
           kinase) (GCK family kinase MiNK)
          Length = 1308

 Score = 34.7 bits (78), Expect = 0.17
 Identities = 26/135 (19%), Positives = 56/135 (41%)
 Frame = -1

Query: 548 KMPRSESRSPVIHRKHRDHPKSYALPEKKESEKLVENGXXXXXXXXXXKGSPELTHNINP 369
           ++ + + R P  H KH  H +   + E+KE  + VE                        
Sbjct: 374 QLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEE-----------------QQRRER 416

Query: 368 KDFSPAESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRI 189
           +     E  + R  + ++ L+ ++     ++ QE +++ ++ + + ERL RQ   E   +
Sbjct: 417 EQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYL 476

Query: 188 KEEVTRRFEIYIQKQ 144
           K    ++ +  +QKQ
Sbjct: 477 KSLQQQQQQQQLQKQ 491
>sp|P16947|M49_STRP9 M protein, serotype 49 precursor
          Length = 389

 Score = 34.3 bits (77), Expect = 0.23
 Identities = 18/59 (30%), Positives = 33/59 (55%)
 Frame = -1

Query: 320 LEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIKEEVTRRFEIYIQKQ 144
           LE L+ Q     +K  QE  K++ + K E+E   + +   E++ + EV +R++  +QKQ
Sbjct: 95  LEKLEHQSQVAADKHYQEQAKKHQEYKQEQEERQKNQEQLERKYQREVEKRYQEQLQKQ 153
>sp|Q5P9F6|SYT_ANAMM Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
          Length = 636

 Score = 34.3 bits (77), Expect = 0.23
 Identities = 20/68 (29%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
 Frame = -1

Query: 350 ESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIK--EEV 177
           E+G   D D+   L ++D   IE+K+        D  A++ER +R+ W  E+ IK  E++
Sbjct: 101 ENGFYYDFDLSHSLSEKDFEAIERKM-------ADIIAKDERFVREVWpreRAIKFFEDL 153

Query: 176 TRRFEIYI 153
              +++ I
Sbjct: 154 GEHYKLEI 161
>sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ATPase
          Length = 864

 Score = 33.9 bits (76), Expect = 0.30
 Identities = 23/74 (31%), Positives = 42/74 (56%)
 Frame = -1

Query: 365 DFSPAESGEMRDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIK 186
           +F      ++R+ D +E    QD N  +K V+E R R ++ K ++E+L  +    EKRIK
Sbjct: 166 EFRKNLENKLRELDRIE----QDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIK 221

Query: 185 EEVTRRFEIYIQKQ 144
            ++  +F+ Y +K+
Sbjct: 222 -DIKDQFDEYEKKR 234
>sp|P26805|GAG_MLVFP Gag polyprotein (Core polyprotein) [Contains: Matrix protein p15;
           RNA binding phosphoprotein p12; Capsid protein p30;
           Nucleocapsid protein p10]
          Length = 538

 Score = 33.9 bits (76), Expect = 0.30
 Identities = 17/56 (30%), Positives = 33/56 (58%)
 Frame = -1

Query: 335 RDSDVLEDLKDQDLNEIEKKVQETRKRNVDPKAEEERLIRQKWAEEKRIKEEVTRR 168
           R  + LEDLK++ L ++ ++ ++   +   P+  EER+ R+   +E+R + E  RR
Sbjct: 407 RKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERVRRETEEKEERRRAEDERR 462
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,192,347
Number of Sequences: 369166
Number of extensions: 665720
Number of successful extensions: 3779
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3387
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3734
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)