Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_J09
(917 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 170 6e-42
sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60) 170 6e-42
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 138 2e-32
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 120 4e-27
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 119 9e-27
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 114 3e-25
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 113 9e-25
sp|P22488|IFEA_HELAS Non-neuronal cytoplasmic intermediate ... 110 4e-24
sp|P16275|IFEB_HELPO Non-neuronal cytoplasmic intermediate ... 108 2e-23
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 108 2e-23
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 170 bits (430), Expect = 6e-42
Identities = 84/219 (38%), Positives = 137/219 (62%)
Frame = +1
Query: 259 TGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIK 438
TGVN +K RE+EK++++ LNERFA+YIEK R+LE QNK+L GE+E LK +TS IK
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIK 143
Query: 439 EIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQR 618
E+YE E+ + R L++ + K + L++++ L +++ QK L + + I +Q
Sbjct: 144 EMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIARQN 203
Query: 619 RELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQ 798
++L EG++ LRR + E + ++ + + DE++K+R D E + H+ E+ Q
Sbjct: 204 QQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAENRRQ 263
Query: 799 TLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
TL E +EF+K +H E+KEL +AYRD + EN +W++E
Sbjct: 264 TLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNE 302
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
Length = 511
Score = 170 bits (430), Expect = 6e-42
Identities = 84/219 (38%), Positives = 137/219 (62%)
Frame = +1
Query: 259 TGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIK 438
TGVN +K RE+EK++++ LNERFA+YIEK R+LE QNK+L GE+E LK +TS IK
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIK 143
Query: 439 EIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQR 618
E+YE E+ + R L++ + K + L++++ L +++ QK L + + I +Q
Sbjct: 144 EMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIARQN 203
Query: 619 RELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQ 798
++L EG++ LRR + E + ++ + + DE++K+R D E + H+ E+ Q
Sbjct: 204 QQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAENRRQ 263
Query: 799 TLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
TL E +EF+K +H E+KEL +AYRD + EN +W++E
Sbjct: 264 TLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNE 302
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 138 bits (347), Expect = 2e-32
Identities = 75/220 (34%), Positives = 128/220 (58%)
Frame = +1
Query: 256 QTGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKI 435
Q ++I+ RE+EKK + LN+R ASYIEK R+LE QN++L +++ L+ DT I
Sbjct: 56 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNI 115
Query: 436 KEIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQ 615
+ +YE E+ + L++E+ K R +E Q+ ++ E+ EM+++L Q R I+
Sbjct: 116 RNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDAL 175
Query: 616 RRELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDL 795
L E ++ L+R E + + KQE L E+++ R D ++E L I ++ +
Sbjct: 176 LVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQV 235
Query: 796 QTLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
QTL E I+F +++H E+KEL T+A RD + EN ++++E
Sbjct: 236 QTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNE 275
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 120 bits (302), Expect = 4e-27
Identities = 68/220 (30%), Positives = 126/220 (57%)
Frame = +1
Query: 256 QTGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKI 435
Q ++I+ RE+EKK + LN+R ASYIEK R+L+ QN++L +++ L+G T +
Sbjct: 58 QNAASTIRDNREREKKEIMELNDRLASYIEKVRFLDAQNRKLDADLKMLQGRFGKSTGSV 117
Query: 436 KEIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQ 615
K +YE+EI +++ ++ K E +I L+ ++DE++K+ + ++ R I+
Sbjct: 118 KVMYEMEITTATNVVKQTGKDHGETEKEIRKLQDQLDELRKKFEEAQRGRAEDRLKIDDL 177
Query: 616 RRELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDL 795
L E ++ L+R E + + K+E L E++++R + ++E L I ++ +
Sbjct: 178 LVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRIELDQETLLRIDNQNKV 237
Query: 796 QTLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
+T+ E I+F K+ TE+KEL A RD + EN Y+++E
Sbjct: 238 KTILEEIDFMKRGFETELKELQAQAARDTTSENREYFKNE 277
Score = 41.2 bits (95), Expect = 0.004
Identities = 37/190 (19%), Positives = 88/190 (46%), Gaps = 10/190 (5%)
Frame = +1
Query: 316 LNERFASYIEKARYLEEQNKQLVGEIETLKGG-------NIDDTSKIKEIYEVEINQLRS 474
L R A E+ L+++N +L E++ ++ ID+ +K+K I E R
Sbjct: 191 LKRRIALLEEEVARLKKENFRLTSELQRVRIELDQETLLRIDNQNKVKTILEEIDFMKRG 250
Query: 475 LLNESEK--SRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQRRELET-FEGD 645
E ++ ++A + + EY +E+ + DI ++Y + R ++E+ ++
Sbjct: 251 FETELKELQAQAARDTTSENREYFKNELANAM---RDIRAEYDQIMNGNRNDMESWYQLR 307
Query: 646 LVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQTLRETIEFE 825
+ E+ + +++ +K+E LR++ +LRQ ++ LE ++ L +E +
Sbjct: 308 VQEINTQSNRQNAENNYQKEEVKRLRNQTSELRQKLSDLESRNLLLEKQIEDLNYQLEDD 367
Query: 826 KQIHTTEMKE 855
++ + + +
Sbjct: 368 QRSYEAALND 377
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 119 bits (299), Expect = 9e-27
Identities = 69/216 (31%), Positives = 119/216 (55%)
Frame = +1
Query: 268 NSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIKEIY 447
++I++ R +EKK + LN+R ASYIEK R+LE QN+++ ++ L+G D++ +K +Y
Sbjct: 61 SAIRESRTREKKEMSELNDRLASYIEKVRFLEAQNRKMEKDLNLLRGKWGHDSTSVKVMY 120
Query: 448 EVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQRREL 627
E E+ + L+ +S+K RA LE QI L E++ + +L E R ++ L
Sbjct: 121 ETELRSAKDLIADSDKERAQLEDQIKKLVEELNNYRNKLHEAERGADVTRKELDNVLGRL 180
Query: 628 ETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQTLR 807
E ++ L+R E K+E + D + R E+E L I ++ +QTL
Sbjct: 181 GALEAEIELLKRRISLMEESIGHLKRENHRMIDSLHHARNAVEQETLNRIDYQNQVQTLL 240
Query: 808 ETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
E +F ++IH +E+ +L +A RD + EN Y+++E
Sbjct: 241 EECDFIRRIHDSEIHDLLAMASRDTTVENREYFKNE 276
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 114 bits (286), Expect = 3e-25
Identities = 65/203 (32%), Positives = 114/203 (56%)
Frame = +1
Query: 307 LQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIKEIYEVEINQLRSLLNE 486
+ LN+R ASYIEK R+LE QN++L +++ L+G DT ++ +YE E+ + R L+N+
Sbjct: 1 MSDLNDRLASYIEKVRFLEAQNRKLAADLDLLRGRWGKDTLSVRAMYEGELQEARKLVND 60
Query: 487 SEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQRRELETFEGDLVELRRF 666
+ + R LE +I L E+ E +++ + R I++ +L E ++ LRR
Sbjct: 61 TNRQREELEKEIRKLLDELSEYRRKYEDALRGHQIDRDRIDELLNQLSGLEAEINLLRRR 120
Query: 667 KGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQTLRETIEFEKQIHTTE 846
+ + K+E +E+ K R D ++E L I ++ +QTL E I+F +++H E
Sbjct: 121 IANIIEEIARIKKENLQFANELTKARSDLDQETLNRIDFQNQVQTLLEEIDFMRRVHDQE 180
Query: 847 MKELTTVAYRDPSKENLVYWQSE 915
+ EL +A RD + EN Y+++E
Sbjct: 181 IAELQAMASRDTTPENREYFKNE 203
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 113 bits (282), Expect = 9e-25
Identities = 65/220 (29%), Positives = 122/220 (55%)
Frame = +1
Query: 256 QTGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKI 435
Q ++I+ RE+EKK + LN+R ASYI K R+L QN++L ++ L+ T +
Sbjct: 58 QGAASTIRDDREREKKEITELNDRLASYIGKVRFLAAQNRKLEADLNVLQSRFGKSTGSV 117
Query: 436 KEIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQ 615
K +YE+EI +++ E+ K E +I ++ ++DE++K+ + ++ R I++
Sbjct: 118 KIMYEMEITTATNVVKETGKDHEEAEKEIGKIKDQLDELRKKFEEAQKGRAEDRLKIDEL 177
Query: 616 RRELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDL 795
L E ++ L+R E + + K+E L E++++R + ++E L I ++ +
Sbjct: 178 LVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRSELDQETLLRIDNQNKV 237
Query: 796 QTLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
T+ E I+F K+ TE+K+L A RD + EN Y+++E
Sbjct: 238 TTILEEIDFMKRGFETELKDLQAQAARDTTSENREYFKNE 277
>sp|P22488|IFEA_HELAS Non-neuronal cytoplasmic intermediate filament protein (IF)
Length = 576
Score = 110 bits (276), Expect = 4e-24
Identities = 64/219 (29%), Positives = 118/219 (53%)
Frame = +1
Query: 259 TGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIK 438
+G+ + RE+EK+ +Q LNER ASYIEK +L+ Q K+L E E L+ + I+
Sbjct: 61 SGITDFRGTREKEKREMQNLNERLASYIEKVHFLDAQVKKLEAENEALRNRKSESLQPIR 120
Query: 439 EIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQR 618
+ YE E+ Q R +++E ++ E ++ L+ EI +++ ++ E+ + YR IE
Sbjct: 121 DAYENELAQARKVIDELSSTKGVSEAKVAGLQDEIASLRELIVTYENQSKDYRKKIESLG 180
Query: 619 RELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQ 798
++ +EG+L LR G+ E ++ K ++ ++++ ++LR D + E AHI + Q
Sbjct: 181 NQIGEYEGELHTLRIRCGSLEDENAKVRELLDKIQEQNRRLRADLDTETAAHIEADCLAQ 240
Query: 799 TLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
T E EF K + +++ L + + +W+SE
Sbjct: 241 TKTEEAEFYKDL-LDQLELLKPEPIQIKGMDYAEFWKSE 278
>sp|P16275|IFEB_HELPO Non-neuronal cytoplasmic intermediate filament protein B (IFB)
Length = 450
Score = 108 bits (271), Expect = 2e-23
Identities = 64/219 (29%), Positives = 116/219 (52%)
Frame = +1
Query: 259 TGVNSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKGGNIDDTSKIK 438
+G+ + RE+EK+ +Q LNER A YIEK +L+ Q K+L E E L+ + I+
Sbjct: 59 SGITDFRGTREKEKREMQNLNERLAGYIEKVHFLDAQVKKLEAENEALRNRKSESLQPIR 118
Query: 439 EIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINSQYRGTIEKQR 618
+ YE E+ Q R +++E ++ E ++ L+ EI +++ + E+ + YR IE
Sbjct: 119 DAYENELAQARKVIDELSSTKGVSEAKVAGLQDEIASLRELIGTYENQSKDYRKKIESLG 178
Query: 619 RELETFEGDLVELRRFKGTREVDSDKEKQETSVLRDEIKKLRQDYEREALAHITLEHDLQ 798
++ +EG+L LR G+ E ++ K ++ ++D+ ++LR D + E AHI + Q
Sbjct: 179 NQIGEYEGELHTLRIRCGSLEDENAKVRELLDKIQDQNRRLRADLDTETSAHIEADCLAQ 238
Query: 799 TLRETIEFEKQIHTTEMKELTTVAYRDPSKENLVYWQSE 915
T E EF K + +++ L + + +W+SE
Sbjct: 239 TKTEECEFYKDL-LDQLELLKPEPMQIKGMDYAEFWKSE 276
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 108 bits (270), Expect = 2e-23
Identities = 64/222 (28%), Positives = 121/222 (54%), Gaps = 4/222 (1%)
Frame = +1
Query: 232 IRGDSVALQTGV-NSIKKGREQEKKNLQGLNERFASYIEKARYLEEQNKQLVGEIETLKG 408
I G S AL S + ++EKK +QGLN+R +YI++ + LEEQN++LV +++ L+G
Sbjct: 59 IGGISPALSANAAKSFLEATDKEKKEMQGLNDRLGNYIDRVKKLEEQNRKLVADLDELRG 118
Query: 409 GNIDDTSKIKEIYEVEINQLRSLLNESEKSRAPLEIQINSLEYEIDEMQKRLMLLEDINS 588
DTS+IK Y + R +++ + +A +++++ L ++ E++ R ED+
Sbjct: 119 RWGKDTSEIKIQYSDSLRDARKEIDDGARRKAEIDVKVARLRDDLAELRNR---YEDVQH 175
Query: 589 QYRGTIEKQRRELETFEGDLVELRRFKGTREVDSDKEKQ---ETSVLRDEIKKLRQDYER 759
+ EK + E EL + +++EK+ + + + +E++K R D +
Sbjct: 176 RRESDREKINQWQHAIEDAQSELEMLRARWRQLTEEEKRLNGDNARIWEELQKARNDLDE 235
Query: 760 EALAHITLEHDLQTLRETIEFEKQIHTTEMKELTTVAYRDPS 885
E L I ++ +QTL E +EF +++H E+KEL + + P+
Sbjct: 236 ETLGRIDFQNQVQTLMEELEFLRRVHEQEVKELQALLAQAPA 277
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.310 0.130 0.344
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,441,082
Number of Sequences: 369166
Number of extensions: 1307884
Number of successful extensions: 5769
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5517
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9366655350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)