Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_I20
(890 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 53 1e-06
sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane... 46 2e-04
sp|Q8QZY6|TSN14_MOUSE Tetraspanin-14 (Tspan-14) (Transmembr... 46 2e-04
sp|Q8NG11|TSN14_HUMAN Tetraspanin-14 (Tspan-14) (Transmembr... 45 2e-04
sp|P19331|IM23_SCHMA 23 kDa integral membrane protein (SM23) 44 8e-04
sp|Q9D7W4|TSN17_MOUSE Tetraspanin-17 (Tspan-17) (Transmembr... 43 0.001
sp|Q96FV3|TSN17_HUMAN Tetraspanin-17 (Tspan-17) (Transmembr... 43 0.001
sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23) 42 0.002
sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23) 41 0.005
sp|Q7YQL0|TSN7_PANTR Tetraspanin-7 (Tspan-7) (Transmembrane... 39 0.015
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 52.8 bits (125), Expect = 1e-06
Identities = 50/211 (23%), Positives = 97/211 (45%), Gaps = 20/211 (9%)
Frame = +1
Query: 274 HIFI--DSLKHIF-NVHSVFGTMIIVALVVITISLSGLYCLYKNDYEKLGLQGASFLILL 444
++FI D H F +V+++F ++I+A+ + + + C + GL F++LL
Sbjct: 33 YVFITYDDYDHFFEDVYTLFPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVFILLL 92
Query: 445 I-------VMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRG 603
+ V++ ++ VE E + Y T + + SR ++ +Q+ L+CCG
Sbjct: 93 VFVTEVVVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNSDAASRAIDYVQRQLHCCGIHN 152
Query: 604 PKDFDVANQY--FVRGMVPISCCNTIANSEICSKFAKQKGYLAINASYSFPIGC----MT 765
D++ + + VP+SCC A S C+ G LA N S + GC +
Sbjct: 153 YSDWENTDWFKETKNQSVPLSCCRETAKS--CN------GSLA-NPSDLYAEGCEALVVK 203
Query: 766 KLEKI----VWPSIVTVILNLSILSFICMII 846
KL++I +W ++ + L + C+++
Sbjct: 204 KLQEILMHVIWAALAFAAIQLLGMLCACIVL 234
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A)
Length = 253
Score = 45.8 bits (107), Expect = 2e-04
Identities = 48/213 (22%), Positives = 99/213 (46%), Gaps = 22/213 (10%)
Frame = +1
Query: 274 HIFI--DSLKHIF-NVHSVFGTMIIVALVVITISLSGLYCLYKNDYEKLGLQGASFLILL 444
++FI D H F +V+++ ++I+A+ + + + C + GL A+F+I+L
Sbjct: 33 YVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGL--ATFVIIL 90
Query: 445 ---------IVMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGS 597
+V++ ++ VE E + Y T + + SR ++ +Q+ L+CCG
Sbjct: 91 LLVFVTEVVVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGI 150
Query: 598 RGPKDFDVANQY--FVRGMVPISCCNTIANSEICSKFAKQKGYLAINASYSFPIGC---- 759
D++ + + VP+SCC A++ C+ G LA + S + GC
Sbjct: 151 HNYSDWENTDWFKETKNQSVPLSCCRETASN--CN------GSLA-HPSDLYAEGCEALV 201
Query: 760 MTKLEKI----VWPSIVTVILNLSILSFICMII 846
+ KL++I +W ++ + L + C+++
Sbjct: 202 VKKLQEIMMHVIWAALAFAAIQLLGMLCACIVL 234
>sp|Q8QZY6|TSN14_MOUSE Tetraspanin-14 (Tspan-14) (Transmembrane 4 superfamily member 14)
Length = 270
Score = 45.8 bits (107), Expect = 2e-04
Identities = 38/146 (26%), Positives = 66/146 (45%), Gaps = 18/146 (12%)
Frame = +1
Query: 283 IDSLKHIFNVHSVFGTMII--VALVVITISLSGLY-------CLYKNDYEKLGLQGASFL 435
+ L + +H + +++ V +V+ T+ +G CL K + L FL
Sbjct: 47 LSDLTKVTRLHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLKFFCGAIVL--IFFL 104
Query: 436 ILLIVMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPKDF 615
L + ++A +F WV F + +SY ++ ++S+QK CCG+ GP+D+
Sbjct: 105 ELAVAVLAFLFQDWVRDRFREFFESNI--KSYRDDIDLQNLIDSLQKANQCCGAYGPEDW 162
Query: 616 DVANQYF---------VRGMVPISCC 666
D+ N YF + VP SCC
Sbjct: 163 DL-NVYFNCSGASYSREKCGVPFSCC 187
>sp|Q8NG11|TSN14_HUMAN Tetraspanin-14 (Tspan-14) (Transmembrane 4 superfamily member 14)
(DC-TM4F2)
Length = 270
Score = 45.4 bits (106), Expect = 2e-04
Identities = 35/144 (24%), Positives = 65/144 (45%), Gaps = 16/144 (11%)
Frame = +1
Query: 283 IDSLKHIFNVHSVFGTMII--VALVVITISLSGLYCLYKNDYEKLGLQGAS-----FLIL 441
+ L + +H + +++ V +V+ T+ +G + + L + FL L
Sbjct: 47 LSDLTKVTRMHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLIFFLEL 106
Query: 442 LIVMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPKDFDV 621
+ ++A +F WV F + +SY ++ ++S+QK CCG+ GP+D+D+
Sbjct: 107 AVAVLAFLFQDWVRDRFREFFESNI--KSYRDDIDLQNLIDSLQKANQCCGAYGPEDWDL 164
Query: 622 ANQYF---------VRGMVPISCC 666
N YF + VP SCC
Sbjct: 165 -NVYFNCSGASYSREKCGVPFSCC 187
>sp|P19331|IM23_SCHMA 23 kDa integral membrane protein (SM23)
Length = 218
Score = 43.5 bits (101), Expect = 8e-04
Identities = 33/128 (25%), Positives = 62/128 (48%), Gaps = 9/128 (7%)
Frame = +1
Query: 307 NVHSVFGT----MIIVALVVITISLSGLYCLYKNDYEKLGLQGASFLILLIV-----MIA 459
N+H V+ +I+V ++++ +S G K + L + ++LLI ++A
Sbjct: 46 NLHKVWQAAPIAIIVVGVIILIVSFLGCCGAIKENVCMLYMYAFFLVVLLIAELAAAIVA 105
Query: 460 LIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPKDFDVANQYFV 639
+++ ++ E +A M+ A P E + ++ IQ +CCG++GP D+
Sbjct: 106 VVYKDRIDSEIDAL--MTGALDK--PTKEITEFMNLIQSSFHCCGAKGPDDY-------- 153
Query: 640 RGMVPISC 663
RG VP SC
Sbjct: 154 RGNVPASC 161
>sp|Q9D7W4|TSN17_MOUSE Tetraspanin-17 (Tspan-17) (Transmembrane 4 superfamily member 17)
(Tetraspan protein SB134) (F-box only protein 23)
Length = 270
Score = 43.1 bits (100), Expect = 0.001
Identities = 35/139 (25%), Positives = 64/139 (46%), Gaps = 14/139 (10%)
Frame = +1
Query: 430 FLILLIVMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPK 609
FL L ++A +F W+ + N + ++Y ++ ++ Q++ CCG+RGP
Sbjct: 105 FLELAAGILAFVFKDWIRDQLNLFINNN--VKAYRDDLDLQNLIDFAQEYWSCCGARGPN 162
Query: 610 DFDVANQYF---------VRGMVPISCC-----NTIANSEICSKFAKQKGYLAINASYSF 747
D+++ N YF R VP SCC + N++ C + K L S +
Sbjct: 163 DWNL-NIYFNCTDLNPSRERCGVPFSCCVRDPAEDVLNTQ-CGYDIRLKLELEQEGSI-Y 219
Query: 748 PIGCMTKLEKIVWPSIVTV 804
GC+ + EK + +++ V
Sbjct: 220 TKGCVGQFEKWLQDNLIVV 238
>sp|Q96FV3|TSN17_HUMAN Tetraspanin-17 (Tspan-17) (Transmembrane 4 superfamily member 17)
(Tetraspan protein SB134) (F-box only protein 23)
Length = 263
Score = 43.1 bits (100), Expect = 0.001
Identities = 35/144 (24%), Positives = 65/144 (45%), Gaps = 19/144 (13%)
Frame = +1
Query: 430 FLILLIVMIALIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPK 609
FL L ++A +F W+ + N + ++Y ++ ++ Q++ CCG+RGP
Sbjct: 105 FLELATGILAFVFKDWIRDQLNLFINNN--VKAYRDDIDLQNLIDFAQEYWSCCGARGPN 162
Query: 610 DFDVANQYF---------VRGMVPISCC---------NTIANSEICSKF-AKQKGYLAIN 732
D+++ N YF R VP SCC NT ++ K +Q+G++
Sbjct: 163 DWNL-NIYFNCTDLNPSRERCGVPFSCCVRDPAEDVLNTQCGYDVRLKLELEQQGFIHTK 221
Query: 733 ASYSFPIGCMTKLEKIVWPSIVTV 804
GC+ + EK + +++ V
Sbjct: 222 -------GCVGQFEKWLQDNLIVV 238
>sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23)
Length = 218
Score = 42.0 bits (97), Expect = 0.002
Identities = 33/128 (25%), Positives = 62/128 (48%), Gaps = 9/128 (7%)
Frame = +1
Query: 307 NVHSVFGT----MIIVALVVITISLSGLYCLYKNDYEKLGLQGASFLILLIV-----MIA 459
N+H V+ +I+V +V++ +S G K + L + ++LLI ++A
Sbjct: 46 NLHKVWQAAPIAIIVVGVVILIVSFLGCCGAIKENVCMLYMYAFFLIVLLIAELVAAIVA 105
Query: 460 LIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPKDFDVANQYFV 639
+++ ++ E N M+ A ++ P E + ++ IQ +CCG +GP D+
Sbjct: 106 VVYKDKIDDEINTL--MTGALEN--PNEEITATMDKIQTSFHCCGVKGPDDY-------- 153
Query: 640 RGMVPISC 663
+G VP SC
Sbjct: 154 KGNVPASC 161
>sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23)
Length = 218
Score = 40.8 bits (94), Expect = 0.005
Identities = 27/112 (24%), Positives = 60/112 (53%), Gaps = 9/112 (8%)
Frame = +1
Query: 307 NVHSVFGT----MIIVALVVITISLSGLYCLYKNDYEKLGLQGASFLILLIV-----MIA 459
N+H V+ +I+V ++++ +S G K + L + +ILLI ++A
Sbjct: 46 NLHKVWQAAPIAIIVVGVIILIVSFLGCCGAIKENVCMLYMYAFFLIILLIAELAAAIVA 105
Query: 460 LIFTFWVEWEANAHFGMSYATQSYSPKMEDSRYLESIQKFLYCCGSRGPKDF 615
+++ ++ E +A M+ A +P++ + +++ IQ +CCG++GP+D+
Sbjct: 106 VVYKDRIDSEIDAL--MTGALDKPTPEITE--FMDLIQSSFHCCGAKGPQDY 153
>sp|Q7YQL0|TSN7_PANTR Tetraspanin-7 (Tspan-7) (Transmembrane 4 superfamily member 2)
Length = 244
Score = 39.3 bits (90), Expect = 0.015
Identities = 25/80 (31%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = +1
Query: 433 LILLIVMIALIFTFWVEWEANAHFGMSY--ATQSYSPKMEDSRYLESIQKFLYCCGSRGP 606
L+ L ++A I F E F +Y A Q+Y+ E SR ++ +Q+ L CCG +
Sbjct: 92 LVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSCCGVQNY 151
Query: 607 KDFDVANQYFVRGMVPISCC 666
++ + YF+ +P SCC
Sbjct: 152 TNWS-TSPYFLEHGIPPSCC 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,390,139
Number of Sequences: 369166
Number of extensions: 1874319
Number of successful extensions: 4621
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4618
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)