Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_I16
(763 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (... 259 4e-69
sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (... 259 4e-69
sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (... 248 1e-65
sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (... 215 1e-55
sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (... 214 3e-55
sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (... 100 4e-21
sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Tra... 56 9e-08
sp|Q8T6B9|PUF68_DROME Poly U binding splicing factor half p... 56 1e-07
sp|P19684|ROC5_NICSY 33 kDa ribonucleoprotein, chloroplast ... 55 2e-07
sp|P48810|RB87F_DROME Heterogeneous nuclear ribonucleoprote... 54 4e-07
>sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(hU2AF(65))
Length = 475
Score = 259 bits (663), Expect = 4e-69
Identities = 123/229 (53%), Positives = 166/229 (72%), Gaps = 2/229 (0%)
Frame = +3
Query: 81 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGSVVNMGLTAAEYPSIGNTVS 260
KK KYWDV P G+E+++ ++K + + GQ+ + L + + +T P +G+ ++
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMT 145
Query: 261 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSV 440
RQARR+YVGN+P G+TEE MM+FFN Q++L TQA G+P++AVQIN DKNFAFLEFRSV
Sbjct: 146 RQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSV 205
Query: 441 EETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGG 614
+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GG
Sbjct: 206 DETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGG 265
Query: 615 LPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
LP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD
Sbjct: 266 LPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTD 314
>sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 475
Score = 259 bits (663), Expect = 4e-69
Identities = 123/229 (53%), Positives = 166/229 (72%), Gaps = 2/229 (0%)
Frame = +3
Query: 81 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGSVVNMGLTAAEYPSIGNTVS 260
KK KYWDV P G+E+++ ++K + + GQ+ + L + + +T P +G+ ++
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMT 145
Query: 261 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSV 440
RQARR+YVGN+P G+TEE MM+FFN Q++L TQA G+P++AVQIN DKNFAFLEFRSV
Sbjct: 146 RQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSV 205
Query: 441 EETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGG 614
+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GG
Sbjct: 206 DETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGG 265
Query: 615 LPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
LP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD
Sbjct: 266 LPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTD 314
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 416
Score = 248 bits (634), Expect = 1e-65
Identities = 124/233 (53%), Positives = 164/233 (70%), Gaps = 6/233 (2%)
Frame = +3
Query: 81 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGSVVNMGLTAAEYPSIGNTVS 260
+K YWDV P G+E+++ ++K + + GQ+ S+ V + TA P +G+T++
Sbjct: 38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTAV--PVVGSTIT 89
Query: 261 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSV 440
RQARR+YVGN+P GVTEE MMEFFN+Q+ L QA G P++A QIN DKNFAFLEFRS+
Sbjct: 90 RQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSI 149
Query: 441 EETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKL 602
+ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+
Sbjct: 150 DETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKI 209
Query: 603 FVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD
Sbjct: 210 FIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITD 262
>sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 488
Score = 215 bits (547), Expect = 1e-55
Identities = 112/226 (49%), Positives = 151/226 (66%)
Frame = +3
Query: 84 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGSVVNMGLTAAEYPSIGNTVSR 263
K Y++WDV PTG+EN++ E+K + + G V GSV + P +G +V+
Sbjct: 125 KKYRFWDVPPTGFENITPMEYKNMQASGAV------PRGSV------QSAVPVVGPSVTC 172
Query: 264 QARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSVE 443
Q+RR+YVGN+P G EE M++FFN+Q+ L QA G+PI+ QIN DKNFAF+EFRS++
Sbjct: 173 QSRRLYVGNIPFGCNEEAMLDFFNQQMHLCNLAQAPGNPILLCQINLDKNFAFIEFRSID 232
Query: 444 ETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQ 623
ET G+ DGI + G LK+RRPRDY P FD + + + IV D+ +K+F+GGLP
Sbjct: 233 ETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNARMPVSSIVVDSANKIFIGGLPN 291
Query: 624 YLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD
Sbjct: 292 YLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTD 336
>sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 496
Score = 214 bits (544), Expect = 3e-55
Identities = 113/226 (50%), Positives = 150/226 (66%)
Frame = +3
Query: 84 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGSVVNMGLTAAEYPSIGNTVSR 263
K Y++WDV PTG+E + E+K + + GQV GSV + P +G +V+
Sbjct: 134 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV------PRGSV------QSAVPVVGPSVTC 181
Query: 264 QARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSVE 443
Q+RR+YVGN+P G EE M++FFN+Q+ L QA G+PI+ QIN DKNFAF+EFRS++
Sbjct: 182 QSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSID 241
Query: 444 ETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQ 623
ET G+ DGI + G LK+RRPRDY P FD + S + IV D+ +K+F+GGLP
Sbjct: 242 ETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPN 300
Query: 624 YLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD
Sbjct: 301 YLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTD 345
>sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (U2 auxiliary factor 59 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF59)
Length = 517
Score = 100 bits (249), Expect = 4e-21
Identities = 75/242 (30%), Positives = 114/242 (47%), Gaps = 13/242 (5%)
Frame = +3
Query: 66 PLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSL---------YLTEGSVVNMG 218
P+ + K+ WD+ P GYE ++A + K SG LP L E + G
Sbjct: 124 PINQWKRKRSLWDIKPPGYELVTADQAKM--SGVFPLPGAPRAAVTDPEKLLEFARSAEG 181
Query: 219 LTAAEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQI 398
A P + SRQARR+ V +P E+ + F + Y + E +V +
Sbjct: 182 SIIAPPPPLQPGASRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNV 241
Query: 399 NTDKNFAFLEFRSVEETNQGLNLDGIVY-HGFALKLRRPRDYVPLPQAFDGIV---SNSM 566
++NFA LE + E+ L Y + LK +R ++Y+ +PQ + S+
Sbjct: 242 CKEENFAILEVATPEDATFLWGLQSESYSNDVFLKFQRIQNYI-VPQITPEVSQKRSDDY 300
Query: 567 TAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYAD 746
V D+ K+++ LP LGEDQV ELL+ FG L F L+K+ G SKG+ FCE+ +
Sbjct: 301 AKNDVLDSKDKIYISNLPLNLGEDQVVELLKPFGDLLSFQLIKNIADGSSKGFCFCEFKN 360
Query: 747 HS 752
S
Sbjct: 361 PS 362
>sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Transcription termination factor
ctf1)
Length = 363
Score = 56.2 bits (134), Expect = 9e-08
Identities = 29/66 (43%), Positives = 37/66 (56%)
Frame = +3
Query: 561 SMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEY 740
SMTAG V +FVG +P + E Q+ E+ GP++ F LV D TG KGY FCE+
Sbjct: 2 SMTAGNV------VFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEF 55
Query: 741 ADHSVT 758
D T
Sbjct: 56 FDSETT 61
Score = 33.1 bits (74), Expect = 0.84
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +3
Query: 276 IYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSVEET 449
++VGN+P V+E+ M E FN+ +K + ++ + + K + F EF E T
Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTFKL-----VLDPETGSGKGYGFCEFFDSETT 61
>sp|Q8T6B9|PUF68_DROME Poly U binding splicing factor half pint (68 kDa poly U binding
splicing factor) (Half pint protein) (PUF60 homolog)
Length = 637
Score = 55.8 bits (133), Expect = 1e-07
Identities = 40/164 (24%), Positives = 73/164 (44%), Gaps = 1/164 (0%)
Frame = +3
Query: 273 RIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRSVEETN 452
R+YVG++ + E+ + F +K + DPI K FAF+E+ E
Sbjct: 131 RVYVGSISFELKEDTIRVAFTPFGPIKSINMS-WDPIT----QKHKGFAFVEYEIPEGAQ 185
Query: 453 QGLN-LDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYL 629
L ++G + G +K+ RP + Q D + + + ++++V + L
Sbjct: 186 LALEQMNGALMGGRNIKVGRPSNMPQAQQVIDEVQEEAKSF-------NRIYVASIHPDL 238
Query: 630 GEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
E+ +K + + FGP+ L + ++ KGY F EYA+ D
Sbjct: 239 SEEDIKSVFEAFGPILYCKLAQGTSLHTHKGYGFIEYANKQAMD 282
>sp|P19684|ROC5_NICSY 33 kDa ribonucleoprotein, chloroplast precursor
Length = 324
Score = 55.1 bits (131), Expect = 2e-07
Identities = 43/166 (25%), Positives = 74/166 (44%), Gaps = 4/166 (2%)
Frame = +3
Query: 258 SRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEFRS 437
S + R+YVGNLP +T + E F E T A + + + + FAF+ S
Sbjct: 110 SVEGGRLYVGNLPFSMTSSQLSEIFAEA-----GTVANVEIVYDRVTDRSRGFAFVTMGS 164
Query: 438 VEETNQGLNL-DGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQ---DTPHKLF 605
VEE + + L DG G +K+ P +P+ + V ++ Q D+PHKL+
Sbjct: 165 VEEAKEAIRLFDGSQVGGRTVKVNFPE----VPRGGEREVMSAKIRSTYQGFVDSPHKLY 220
Query: 606 VGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYA 743
V L L +++ ++ D ++G S+G+ F ++
Sbjct: 221 VANLSWALTSQGLRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFS 266
>sp|P48810|RB87F_DROME Heterogeneous nuclear ribonucleoprotein 87F (HRP36.1 protein) (P11
protein)
Length = 385
Score = 54.3 bits (129), Expect = 4e-07
Identities = 42/172 (24%), Positives = 72/172 (41%), Gaps = 2/172 (1%)
Frame = +3
Query: 252 TVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKEYTQAEGDPIIAVQINTDKNFAFLEF 431
T Q R++++G L T++G+ F + + + + DP + + F F+ +
Sbjct: 18 TEPEQLRKLFIGGLDYRTTDDGLKAHFEKWGNIVDVVVMK-DP----KTKRSRGFGFITY 72
Query: 432 RSVEETNQGLNLDGIVYHGFALKLRR--PRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLF 605
+ N G ++ +R PR + P A T KLF
Sbjct: 73 SQSYMIDNAQNARPHKIDGRTVEPKRAVPRQEIDSPNA--------------GATVKKLF 118
Query: 606 VGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTD 761
VGGL E+ ++E + FG + N+V D TG +G+AF E+ D+ D
Sbjct: 119 VGGLRDDHDEECLREYFKDFGQIVSVNIVSDKDTGKKRGFAFIEFDDYDPVD 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,591,060
Number of Sequences: 369166
Number of extensions: 2047467
Number of successful extensions: 5333
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4867
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5228
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)