Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_G17
(855 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O14733|MP2K7_HUMAN Dual specificity mitogen-activated pr... 187 3e-47
sp|Q23977|HEP_DROME Dual specificity mitogen-activated prot... 186 9e-47
sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated pr... 157 3e-38
sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kina... 155 1e-37
sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated pr... 153 7e-37
sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated pr... 153 7e-37
sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated pr... 152 1e-36
sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated pr... 151 3e-36
sp|Q05116|MP2K1_XENLA Dual specificity mitogen-activated pr... 146 6e-35
sp|Q01986|MP2K1_RAT Dual specificity mitogen-activated prot... 145 1e-34
>sp|O14733|MP2K7_HUMAN Dual specificity mitogen-activated protein kinase kinase 7 (MAP
kinase kinase 7) (MAPKK 7) (MAPK/ERK kinase 7)
(JNK-activating kinase 2) (c-Jun N-terminal kinase
kinase 2) (JNK kinase 2) (JNKK 2)
Length = 419
Score = 187 bits (475), Expect = 3e-47
Identities = 91/182 (50%), Positives = 124/182 (68%), Gaps = 6/182 (3%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ AI+KAL YLK KH ++HRD+KPSN+LL +RG++KLCDFGIS +L DS A +R
Sbjct: 219 LGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSA 278
Query: 181 GCIGYMAPERLN-----SSTYDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDG 345
GC YMAPER++ YDIRADVWSLG+SLVELA G+FP K++FE++ ++
Sbjct: 279 GCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQE 338
Query: 346 KPPSLPDGI-YSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDILIWYKSVN 522
+PP LP + +S F FV CL KD RPKY+KL+ F + + +D+ W+K V
Sbjct: 339 EPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVM 398
Query: 523 HK 528
K
Sbjct: 399 AK 400
>sp|Q23977|HEP_DROME Dual specificity mitogen-activated protein kinase kinase
hemipterous (MAPKK)
Length = 1178
Score = 186 bits (471), Expect = 9e-47
Identities = 89/186 (47%), Positives = 127/186 (68%), Gaps = 5/186 (2%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GKV+ A + AL YLK KH ++HRD+KPSN+L+ +RG +KLCDFGIS +L DS AN+R
Sbjct: 296 LGKVTVATVNALSYLKDKHGVIHRDVKPSNILIDERGNIKLCDFGISGRLVDSKANTRSA 355
Query: 181 GCIGYMAPERLN--SSTYDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGKPP 354
GC YMAPER++ YDIRADVWSLG++LVELA + P +G ++FE++ ++D +PP
Sbjct: 356 GCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATARSPYEGCNTDFEVLTKVLDSEPP 415
Query: 355 SLPDG---IYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDILIWYKSVNH 525
LP G +S+ F FV CL K+ RPKY +L++ F RI+ +D+ W++S+
Sbjct: 416 CLPYGEGYNFSQQFRDFVIKCLTKNHQDRPKYPELLAQPFIRIYESAKVDVPNWFQSIKD 475
Query: 526 KPVVAN 543
+ AN
Sbjct: 476 NRLRAN 481
>sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated protein kinase kinase 2 (MAP
kinase kinase 2) (MAPKK 2) (MAPK-ERK kinase 2)
Length = 446
Score = 157 bits (398), Expect = 3e-38
Identities = 80/173 (46%), Positives = 112/173 (64%), Gaps = 7/173 (4%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ A +KAL +LK I+HRDIKPSN+LL G +KLCDFGIS +L DSIA +R
Sbjct: 252 LGKITLATVKALNHLKENLKIIHRDIKPSNILLDTNGNIKLCDFGISGQLVDSIAKTRDA 311
Query: 181 GCIGYMAPERLNSST----YDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGK 348
GC YMAPER++ S YD+R+DVWSLG++L ELA G+FP S F+ + ++ G
Sbjct: 312 GCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD 371
Query: 349 PPSLPDG---IYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDI 498
PP L + +S +F FV+ CL KD + RPKY +L+ F ++ +D+
Sbjct: 372 PPQLSNSEEREFSPSFTSFVNQCLTKDESKRPKYKELLKHPFILMYEERTVDV 424
>sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kinase F42G10.2
Length = 363
Score = 155 bits (392), Expect = 1e-37
Identities = 80/166 (48%), Positives = 108/166 (65%), Gaps = 5/166 (3%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+G ++ + AL YLK + I+HRD+KPSN+L+ G VKLCDFGI +L++S A +
Sbjct: 170 VGHITVCTVDALDYLKKELKIIHRDVKPSNILVDGTGAVKLCDFGICGQLEESFAKTHDA 229
Query: 181 GCIGYMAPERLNSS-TYDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGKPPS 357
GC Y+APER+ SS YD+R+DVWSLG++L E+A GKFP S F+ +A ++ G PP
Sbjct: 230 GCQPYLAPERITSSDKYDVRSDVWSLGITLYEIATGKFPYQEWNSLFDQIATVVSGDPPI 289
Query: 358 L----PDGIYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNV 483
L D YS F+++CL KD RPKYD L S DFYRI+ V
Sbjct: 290 LHPDSDDFHYSLPLVKFINTCLTKDRRHRPKYDTLKSFDFYRIYAV 335
>sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4)
(JNK-activating kinase 1) (C-JUN N-terminal kinase
kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1)
(SEK1)
Length = 397
Score = 153 bits (386), Expect = 7e-37
Identities = 79/173 (45%), Positives = 112/173 (64%), Gaps = 7/173 (4%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ A +KAL +LK I+HRDIKPSN+LL G +KLCDFGIS +L DSIA +R
Sbjct: 203 LGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDA 262
Query: 181 GCIGYMAPERLNSST----YDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGK 348
GC YMAPER++ S YD+R+DVWSLG++L ELA G+FP S F+ + ++ G
Sbjct: 263 GCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD 322
Query: 349 PPSLPDG---IYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDI 498
PP L + +S +F FV+ CL KD + RPKY +L+ F ++ +++
Sbjct: 323 PPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERTVEV 375
>sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (JNK-activating kinase 1) (c-Jun
N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1)
(SEK1)
Length = 399
Score = 153 bits (386), Expect = 7e-37
Identities = 79/173 (45%), Positives = 112/173 (64%), Gaps = 7/173 (4%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ A +KAL +LK I+HRDIKPSN+LL G +KLCDFGIS +L DSIA +R
Sbjct: 205 LGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDA 264
Query: 181 GCIGYMAPERLNSST----YDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGK 348
GC YMAPER++ S YD+R+DVWSLG++L ELA G+FP S F+ + ++ G
Sbjct: 265 GCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGD 324
Query: 349 PPSLPDG---IYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDI 498
PP L + +S +F FV+ CL KD + RPKY +L+ F ++ +++
Sbjct: 325 PPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEV 377
>sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 152 bits (383), Expect = 1e-36
Identities = 71/177 (40%), Positives = 115/177 (64%), Gaps = 4/177 (2%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ +I+KAL++L +K ++HRD+KPSN+L++ G+VK+CDFGIS L DS+A +
Sbjct: 155 LGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINTLGQVKMCDFGISGYLVDSVAKTIDA 214
Query: 181 GCIGYMAPER----LNSSTYDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGK 348
GC YMAPER LN Y +++D+WSLG++++ELAI +FP D + F+ + +++
Sbjct: 215 GCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEP 274
Query: 349 PPSLPDGIYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDILIWYKSV 519
P LP +S F F S CL+K+ RP Y +L+ F+ +H + D+ + K +
Sbjct: 275 SPQLPADKFSADFVDFTSQCLKKNSKERPTYPELMQHPFFTVHESKAADVASFVKLI 331
>sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 151 bits (381), Expect = 3e-36
Identities = 71/177 (40%), Positives = 115/177 (64%), Gaps = 4/177 (2%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GK++ +I+KAL++L +K ++HRD+KPSN+L++ G+VK+CDFGIS L DS+A +
Sbjct: 155 LGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDA 214
Query: 181 GCIGYMAPER----LNSSTYDIRADVWSLGMSLVELAIGKFPIDGLKSEFEIMACIIDGK 348
GC YMAPER LN Y +++D+WSLG++++ELAI +FP D + F+ + +++
Sbjct: 215 GCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEP 274
Query: 349 PPSLPDGIYSEAFNIFVSSCLQKDFNLRPKYDKLVSADFYRIHNVEPLDILIWYKSV 519
P LP +S F F S CL+K+ RP Y +L+ F+ +H + D+ + K +
Sbjct: 275 SPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLI 331
>sp|Q05116|MP2K1_XENLA Dual specificity mitogen-activated protein kinase kinase 1 (MAP
kinase kinase 1) (MAPKK 1) (ERK activator kinase 1)
(MAPK/ERK kinase 1) (MEK1)
Length = 395
Score = 146 bits (369), Expect = 6e-35
Identities = 82/212 (38%), Positives = 117/212 (55%), Gaps = 43/212 (20%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GKVS A+IK L YL+ KH IMHRD+KPSN+L++ RGE+KLCDFG+S +L DS+ANS
Sbjct: 166 LGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANS-FV 224
Query: 181 GCIGYMAPERLNSSTYDIRADVWSLGMSLVELAIGKFPI------------------DGL 306
G YM+PERL + Y +++D+WS+G+SLVE+AIG++PI D
Sbjct: 225 GTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAIGRYPIPPPDAKELELIFGCSVERDPA 284
Query: 307 KSE-------------------------FEIMACIIDGKPPSLPDGIYSEAFNIFVSSCL 411
SE FE++ I++ PP LP G++ F FV+ CL
Sbjct: 285 SSELAPRPRPPGRPISSYGPDSRPPMAIFELLDYIVNEPPPKLPSGVFGAEFQDFVNKCL 344
Query: 412 QKDFNLRPKYDKLVSADFYRIHNVEPLDILIW 507
K+ R +L+ F + +E +D W
Sbjct: 345 VKNPAERADLKQLMVHSFIKQSELEEVDFAGW 376
>sp|Q01986|MP2K1_RAT Dual specificity mitogen-activated protein kinase kinase 1 (MAP
kinase kinase 1) (MAPKK 1) (ERK activator kinase 1)
(MAPK/ERK kinase 1) (MEK1)
Length = 393
Score = 145 bits (367), Expect = 1e-34
Identities = 81/210 (38%), Positives = 118/210 (56%), Gaps = 41/210 (19%)
Frame = +1
Query: 1 IGKVSCAIIKALQYLKTKHDIMHRDIKPSNMLLSDRGEVKLCDFGISAKLKDSIANSRQP 180
+GKVS A+IK L YL+ KH IMHRD+KPSN+L++ RGE+KLCDFG+S +L DS+ANS
Sbjct: 166 LGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANS-FV 224
Query: 181 GCIGYMAPERLNSSTYDIRADVWSLGMSLVELAIGKFPID-------------------- 300
G YM+PERL + Y +++D+WS+G+SLVE+A+G++PI
Sbjct: 225 GTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCQVEGDAA 284
Query: 301 ----------------GLKSE-----FEIMACIIDGKPPSLPDGIYSEAFNIFVSSCLQK 417
G+ S FE++ I++ PP LP G++S F FV+ CL K
Sbjct: 285 ETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIK 344
Query: 418 DFNLRPKYDKLVSADFYRIHNVEPLDILIW 507
+ R +L+ F + + E +D W
Sbjct: 345 NPAERADLKQLMVHAFIKRSDAEEVDFAGW 374
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,610,972
Number of Sequences: 369166
Number of extensions: 1723965
Number of successful extensions: 7535
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6140
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)