Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_F23
(894 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P10746|HEM4_HUMAN Uroporphyrinogen-III synthase (UROS) (... 132 1e-30
sp|P51163|HEM4_MOUSE Uroporphyrinogen-III synthase (UROS) (... 124 4e-28
sp|P06174|HEM4_YEAST Uroporphyrinogen-III synthase (UROS) (... 82 3e-15
sp|P87214|HEM4_SCHPO Uroporphyrinogen-III synthase (UROS) (... 72 2e-12
sp|Q10234|RT05_SCHPO Probable mitochondrial 40S ribosomal p... 34 0.48
sp|Q8TYR8|Y224_METKA Hypothetical UPF0284 protein MK0224 34 0.63
sp|P64192|G6PI_MYCTU Glucose-6-phosphate isomerase (GPI) (P... 32 2.4
sp|P96803|G6PI_MYCSM Glucose-6-phosphate isomerase (GPI) (P... 32 2.4
sp|Q12873|CHD3_HUMAN Chromodomain helicase-DNA-binding prot... 32 3.1
sp|Q9LFA3|MDA3_ARATH Probable monodehydroascorbate reductas... 31 4.1
>sp|P10746|HEM4_HUMAN Uroporphyrinogen-III synthase (UROS) (Uroporphyrinogen-III
cosynthetase) (Hydroxymethylbilane hydrolyase
[cyclizing]) (UROIIIS)
Length = 265
Score = 132 bits (332), Expect = 1e-30
Identities = 82/247 (33%), Positives = 129/247 (52%), Gaps = 13/247 (5%)
Frame = +2
Query: 8 EDPYVKVLMENNFNVSLIETLDFVFHPEELKSICSNYTQWYENFSAIIFTSPRAVQAFQM 187
+DPY++ L +LI L F F L S + E++ +IFTSPRAV+A ++
Sbjct: 16 QDPYIRELGLYGLEATLIPVLSFEFL--SLPSFSEKLSH-PEDYGGLIFTSPRAVEAAEL 72
Query: 188 SGLPASKNVV-------------CFVVGNATKALAESLGFETAGAESGNADVLLDTIVNY 328
+K V +VVGNAT +L +G +T G GNA+ L + I +
Sbjct: 73 CLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSR 132
Query: 329 DRNALTKPVLFPCGKMHRPVLPKKLEENGIQIKSVFVYSTKESVELSEKLKQLFTNSQNI 508
+ +AL P+LFPCG + R +LPK L++ GI ++S+ VY T + L ++ Q +
Sbjct: 133 ESSAL--PLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQ-QGV 189
Query: 509 PDLLVFFSPSGIAFSEKVLKDEIVSKHSHLKFGSMGPATSAQLTSSGFSVELESRSPKPE 688
P + FFSPSG+ +S K +++ +KF ++GP T+ L + G V + SP P+
Sbjct: 190 PASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQ 249
Query: 689 ALLKAIK 709
AL I+
Sbjct: 250 ALATGIR 256
>sp|P51163|HEM4_MOUSE Uroporphyrinogen-III synthase (UROS) (Uroporphyrinogen-III
cosynthetase) (Hydroxymethylbilane hydrolyase
[cyclizing]) (UROIIIS)
Length = 265
Score = 124 bits (311), Expect = 4e-28
Identities = 81/249 (32%), Positives = 128/249 (51%), Gaps = 13/249 (5%)
Frame = +2
Query: 11 DPYVKVLMENNFNVSLIETLDFVFHPEELKSICSNYTQWYENFSAIIFTSPRAVQAFQMS 190
DPY++ L +LI L F F L S+ + E F +IFTSPRAV+A ++
Sbjct: 17 DPYIQELRLCGLEATLIPVLSFEFM--SLPSLSEKLSH-PEGFGGLIFTSPRAVEAVKLC 73
Query: 191 GLPASKNVV-------------CFVVGNATKALAESLGFETAGAESGNADVLLDTIVNYD 331
+K +VVG+AT +L +G + GA SGNA+ L + I +
Sbjct: 74 LEKDNKTEAWEKSLKDRWNAKSVYVVGSATASLVNKIGLDAEGAGSGNAEKLAEYICSKP 133
Query: 332 RNALTKPVLFPCGKMHRPVLPKKLEENGIQIKSVFVYSTKESVELSEKLKQLFTNSQNIP 511
+ L P+LFPCG + LPK L++ GI ++S+ VY T + LK + + Q IP
Sbjct: 134 SSEL--PLLFPCGTIKGDTLPKMLKDKGIPMESMHVYQTVPHPGIQGSLKSYYED-QGIP 190
Query: 512 DLLVFFSPSGIAFSEKVLKDEIVSKHSHLKFGSMGPATSAQLTSSGFSVELESRSPKPEA 691
+ FFSPSG+ +S + ++ S +KF ++GP+T+ + + G V + SP P+A
Sbjct: 191 ASITFFSPSGLKYSLEYIQALSGSSFDQIKFIAIGPSTTRAMAAKGLPVSCTAESPTPQA 250
Query: 692 LLKAIKGLI 718
L I+ ++
Sbjct: 251 LAAGIRNVL 259
>sp|P06174|HEM4_YEAST Uroporphyrinogen-III synthase (UROS) (Uroporphyrinogen-III
cosynthetase) (Hydroxymethylbilane hydrolyase
[cyclizing]) (UROIIIS)
Length = 275
Score = 81.6 bits (200), Expect = 3e-15
Identities = 63/229 (27%), Positives = 104/229 (45%), Gaps = 22/229 (9%)
Frame = +2
Query: 89 EELKSICSNYTQWYENFSAIIFTSPRAVQAFQMSGLPA----------SKNVVCFVVGNA 238
++ +++ + + + II TS R V++ + +P SK V + VG A
Sbjct: 46 QDFRNVLNTIPNYLNTINYIIITSQRTVESLNEAIIPTLTSEQKAALLSKTV--YTVGPA 103
Query: 239 TKALAESLGF------ETAGAESGNADVLLDTIVNYDRNALTKPVLFPCGKMHRPVLPKK 400
T GF E AG S AD+++D + + +LF G++ R ++PKK
Sbjct: 104 TANFIRRSGFINVKGGEDAGNGSILADIIIDDLSTDIKACPPSELLFLVGEIRRDIIPKK 163
Query: 401 LEENGIQIKSVFVYSTKESVELSEKLKQLF------TNSQNIPDLLVFFSPSGIAFSEKV 562
L GI+++ V Y T+ ELS+ K+ + D +V FSP G +
Sbjct: 164 LHSKGIKVREVVTYKTE---ELSDGFKRFIHAMKECDEDEVFSDWVVVFSPQGTKEITQY 220
Query: 563 LKDEIVSKHSHLKFGSMGPATSAQLTSSGFSVELESRSPKPEALLKAIK 709
L D SHL+ S+GP T L + + ++ S P P++LL AI+
Sbjct: 221 LGDSNRLPGSHLRVASIGPTTKKYLDDNDVTSDVVSPKPDPKSLLDAIE 269
>sp|P87214|HEM4_SCHPO Uroporphyrinogen-III synthase (UROS) (Uroporphyrinogen-III
cosynthetase) (Hydroxymethylbilane hydrolyase
[cyclizing]) (UROIIIS)
Length = 251
Score = 72.4 bits (176), Expect = 2e-12
Identities = 67/243 (27%), Positives = 106/243 (43%), Gaps = 11/243 (4%)
Frame = +2
Query: 11 DPYVKVLMENNFNVSLIETLDFV-FHPEELKSICSNYTQWYENFSAIIFTSPRAVQ---- 175
DPYV+ + + + I L H E+L+ N + Y +I TS R +
Sbjct: 15 DPYVEAFEKYGRDTAFIPVLRHKRVHEEQLRDKLKNVRKTY---CGLIVTSQRVSETLDE 71
Query: 176 AFQMSGLPASKNVVC----FVVGNATKALAESLGFE-TAGAESGNADVLLDTIVN-YDRN 337
A + + ++ F VG AT LGF+ T G + G +VL D I Y
Sbjct: 72 ALKQEDETERQKILMETPIFTVGPATDDSIRRLGFQQTHGKDCGRGEVLADLIEEWYTTT 131
Query: 338 ALTKPVLFPCGKMHRPVLPKKLEENGIQIKSVFVYSTKESVELSEKLKQLFTNSQNIPDL 517
KP+LF G+ HR ++ +KL ++ ++ S+ VY+T+E ++K I D
Sbjct: 132 KQHKPLLFLVGEKHRDIIQRKLGDD--RVDSLIVYATQELENTETQIKDTIRKHPTI-DW 188
Query: 518 LVFFSPSGIAFSEKVLKDEIVSKHSHLKFGSMGPATSAQLTSSGFSVELESRSPKPEALL 697
+V FSP+ I + LK ++GP T L G + S +P PE+L
Sbjct: 189 IVAFSPTSICSLLNTFE---------LKIATIGPTTGDYLKKLGTQPNVVSPAPNPESLA 239
Query: 698 KAI 706
+I
Sbjct: 240 SSI 242
>sp|Q10234|RT05_SCHPO Probable mitochondrial 40S ribosomal protein S5
Length = 387
Score = 34.3 bits (77), Expect = 0.48
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +2
Query: 89 EELKSICSNYTQWYENFSAIIFTSPRAVQAFQMSGLPASKNVVCFVVGNATKALAESLGF 268
EELKS ++ +YEN+ I P A++A Q S P + V K + G+
Sbjct: 106 EELKSRFKDHLPFYENY---IRNHPLAIEALQKSTAPRQTGLKLNYVSKLDKYVTPLDGY 162
Query: 269 ETAGAESGNADV 304
G+E N ++
Sbjct: 163 SHLGSEESNGEI 174
>sp|Q8TYR8|Y224_METKA Hypothetical UPF0284 protein MK0224
Length = 378
Score = 33.9 bits (76), Expect = 0.63
Identities = 37/142 (26%), Positives = 62/142 (43%), Gaps = 13/142 (9%)
Frame = +2
Query: 263 GFETAGAESGNADV----------LLDTIVNYDRNALTKPVLFPCGKMHRPVLPKKLEEN 412
G E G E G+ D ++ +V + T PVL G P+L EE
Sbjct: 221 GLEAQGVEPGDLDAHEAIRRFGDPMMPAVVGIIYGSRT-PVLLAGGTQMAPILAYLAEEG 279
Query: 413 GIQIKSVFVYSTKESVELSEK-LKQLFTNSQNIPDLLVFFSPSGIAFSEKVLKDEIVSKH 589
+ + VFV +TK VE + ++ LF + + D ++F + G FSE + + +
Sbjct: 280 QLDPERVFVGTTKYVVEDEDSDIESLF---RQVGDYVLFSADPG--FSESKFRGFRLYEE 334
Query: 590 SHLK--FGSMGPATSAQLTSSG 649
++K G+ G +A L + G
Sbjct: 335 GYVKEGVGAGGAQVAAALKTKG 356
>sp|P64192|G6PI_MYCTU Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
sp|P64193|G6PI_MYCBO Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
Length = 553
Score = 32.0 bits (71), Expect = 2.4
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -1
Query: 405 SNFFGSTGRCILPQGNNTGLVKAFLS*FTIVSNKTSALPLSAPAVSNPND 256
SNFFG+ R +LP N+ A+L T+ SN S +P ++ +
Sbjct: 327 SNFFGAQSRTVLPYSNDLSRFPAYLQQLTMESNGKSTRADGSPVSADTGE 376
>sp|P96803|G6PI_MYCSM Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
Length = 442
Score = 32.0 bits (71), Expect = 2.4
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = -1
Query: 405 SNFFGSTGRCILPQGNNTGLVKAFLS*FTIVSNKTSALPLSAPAVSNPND 256
SNFFG+ R +LP N+ A+L T+ SN S P ++ +
Sbjct: 216 SNFFGAQSRAVLPYSNDLSRFAAYLQQLTMESNGKSVRADGTPVSTDTGE 265
>sp|Q12873|CHD3_HUMAN Chromodomain helicase-DNA-binding protein 3 (CHD-3) (Mi-2 autoantigen
240 kDa protein) (Mi2-alpha)
Length = 1944
Score = 31.6 bits (70), Expect = 3.1
Identities = 19/55 (34%), Positives = 22/55 (40%)
Frame = +2
Query: 266 FETAGAESGNADVLLDTIVNYDRNALTKPVLFPCGKMHRPVLPKKLEENGIQIKS 430
FE + G V L I+ + P LFP M P LP E G IKS
Sbjct: 1004 FEALNSRGGGNQVSLLNIMMDLKKCCNHPYLFPVAAMESPKLPSGAYEGGALIKS 1058
>sp|Q9LFA3|MDA3_ARATH Probable monodehydroascorbate reductase, cytoplasmic isoform 3
(MDAR 3)
Length = 434
Score = 31.2 bits (69), Expect = 4.1
Identities = 22/88 (25%), Positives = 38/88 (43%), Gaps = 13/88 (14%)
Frame = +2
Query: 200 ASKNVVCFVVGNATKALAESLGFETAGAESGNADVLL-DTIVNYDRNALTKPVLFPCGKM 376
A K+ ++G A + F G + G V+ + + Y+R AL+K LFP G
Sbjct: 2 AEKSFKYIILGGGVSAGYAAKEFANQGVQPGELAVISKEAVAPYERPALSKGYLFPEGAA 61
Query: 377 HRP------------VLPKKLEENGIQI 424
P +LP+ ++ GI++
Sbjct: 62 RLPGFHCCVGSGGEKLLPESYKQKGIEL 89
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,345,927
Number of Sequences: 369166
Number of extensions: 1919237
Number of successful extensions: 4995
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4984
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)