Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_013_E13 (356 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) 36 0.024 sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) 35 0.040 sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog) 32 0.58 sp|Q6GWX0|DDX4_PIG DEAD-box protein 4 (VASA homolog) (VASA-... 32 0.58 sp|Q4R5S7|DDX4_MACFA DEAD-box protein 4 32 0.58 sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) 31 0.76 sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DE... 29 3.8 sp|P16042|NEU4_ONCKE Vasotocin-neurophysin VT 2 precursor [... 28 4.9 sp|Q9JIB4|TF2H2_MOUSE TFIIH basal transcription factor comp... 28 6.5 sp|Q9CM18|SPRT_PASMU Protein sprT 28 8.4
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) Length = 713 Score = 36.2 bits (82), Expect = 0.024 Identities = 22/59 (37%), Positives = 29/59 (49%), Gaps = 8/59 (13%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRR-------DYRKNVE 154 D + D LA VK+L DA Q+ P WLE + S Y P + + R D RKN + Sbjct: 630 DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNSTRGAVFASFDTRKNFQ 688
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) Length = 702 Score = 35.4 bits (80), Expect = 0.040 Identities = 23/60 (38%), Positives = 29/60 (48%), Gaps = 9/60 (15%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRR--------DYRKNVE 154 D D D LA VK+L DA Q+ P WLE + S Y P + + R D RKN + Sbjct: 618 DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRKNYQ 677
>sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog) Length = 724 Score = 31.6 bits (70), Expect = 0.58 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157 D + D LA VK+L DA Q+ P WLE + S Y P R + +V++ Sbjct: 645 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 697
>sp|Q6GWX0|DDX4_PIG DEAD-box protein 4 (VASA homolog) (VASA-like protein) Length = 722 Score = 31.6 bits (70), Expect = 0.58 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157 D + D LA VK+L DA Q+ P WLE + S Y P R + +V++ Sbjct: 643 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 695
>sp|Q4R5S7|DDX4_MACFA DEAD-box protein 4 Length = 725 Score = 31.6 bits (70), Expect = 0.58 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157 D + D LA VK+L DA Q+ P WLE + S Y P R + +V++ Sbjct: 646 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 698
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) Length = 661 Score = 31.2 bits (69), Expect = 0.76 Identities = 16/42 (38%), Positives = 25/42 (59%) Frame = +2 Query: 2 DEDKDVALAPRFVKILKDAGQECPNWLESLCSGYRPSEHNRN 127 D +KD A+A VKIL+ +GQ P++L + +G N+N Sbjct: 594 DPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQN 635
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2) (mDEAD2) (D1Pas1-related sequence 1) Length = 658 Score = 28.9 bits (63), Expect = 3.8 Identities = 17/62 (27%), Positives = 33/62 (53%), Gaps = 11/62 (17%) Frame = +2 Query: 8 DKDVALAPRFVKILKDAGQECPNWLESLC--SGYRPSEHNRNR---------RDYRKNVE 154 ++++ + + +L +A QE P+WLES+ Y+ S R++ RDYR++ Sbjct: 546 ERNLNITKDLLDLLVEAKQEVPSWLESMAYEHHYKGSSRGRSKSRFSGGFGARDYRQSSG 605 Query: 155 SS 160 S+ Sbjct: 606 SA 607
>sp|P16042|NEU4_ONCKE Vasotocin-neurophysin VT 2 precursor [Contains: Vasotocin (VT); Neurophysin VT 2] Length = 158 Score = 28.5 bits (62), Expect = 4.9 Identities = 13/28 (46%), Positives = 14/28 (50%), Gaps = 1/28 (3%) Frame = +1 Query: 10 QGCCISSKVC-KNS*GCWTGMPELAREF 90 QG C +C GCW G PE AR F Sbjct: 49 QGHCFGPSICCGEGLGCWMGSPETARCF 76
>sp|Q9JIB4|TF2H2_MOUSE TFIIH basal transcription factor complex p44 subunit (Basic transcription factor 2 44 kDa subunit) (BTF2-p44) (General transcription factor IIH polypeptide 2) Length = 396 Score = 28.1 bits (61), Expect = 6.5 Identities = 10/32 (31%), Positives = 21/32 (65%) Frame = -1 Query: 212 IKKNHHHLRAQIYFQQMLTIQHFYGSPFCFGC 117 + +++HHL FQ+ ++++ + G FC+GC Sbjct: 319 LARSYHHLFPLDAFQE-ISLEEYKGERFCYGC 349
>sp|Q9CM18|SPRT_PASMU Protein sprT Length = 165 Score = 27.7 bits (60), Expect = 8.4 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +3 Query: 135 TTVKMLNRQHLLKINLCTQMMMIFFNRSFDSMKNH 239 T ++ LNRQ K+ C Q+ +FNR+F H Sbjct: 5 TQLRHLNRQVQRKLTQCLQLASGYFNRTFSMPTVH 39
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 37,088,034 Number of Sequences: 369166 Number of extensions: 648879 Number of successful extensions: 1562 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1544 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1562 length of database: 68,354,980 effective HSP length: 86 effective length of database: 52,467,770 effective search space used: 1678968640 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)