Planarian EST Database


Dr_sW_013_E13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_013_E13
         (356 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q64060|DDX4_RAT  DEAD-box protein 4 (VASA homolog) (rVLG)       36   0.024
sp|Q61496|DDX4_MOUSE  DEAD-box protein 4 (VASA homolog) (Mvh)      35   0.040
sp|Q9NQI0|DDX4_HUMAN  DEAD-box protein 4 (VASA homolog)            32   0.58 
sp|Q6GWX0|DDX4_PIG  DEAD-box protein 4 (VASA homolog) (VASA-...    32   0.58 
sp|Q4R5S7|DDX4_MACFA  DEAD-box protein 4                           32   0.58 
sp|P09052|VASA_DROME  Vasa protein (Antigen Mab46F11)              31   0.76 
sp|Q62095|DDX3Y_MOUSE  DEAD-box protein 3, Y-chromosomal (DE...    29   3.8  
sp|P16042|NEU4_ONCKE  Vasotocin-neurophysin VT 2 precursor [...    28   4.9  
sp|Q9JIB4|TF2H2_MOUSE  TFIIH basal transcription factor comp...    28   6.5  
sp|Q9CM18|SPRT_PASMU  Protein sprT                                 28   8.4  
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG)
          Length = 713

 Score = 36.2 bits (82), Expect = 0.024
 Identities = 22/59 (37%), Positives = 29/59 (49%), Gaps = 8/59 (13%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRR-------DYRKNVE 154
           D + D  LA   VK+L DA Q+ P WLE +  S Y P   + + R       D RKN +
Sbjct: 630 DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNSTRGAVFASFDTRKNFQ 688
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh)
          Length = 702

 Score = 35.4 bits (80), Expect = 0.040
 Identities = 23/60 (38%), Positives = 29/60 (48%), Gaps = 9/60 (15%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRR--------DYRKNVE 154
           D D D  LA   VK+L DA Q+ P WLE +  S Y P   + + R        D RKN +
Sbjct: 618 DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRKNYQ 677
>sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog)
          Length = 724

 Score = 31.6 bits (70), Expect = 0.58
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157
           D + D  LA   VK+L DA Q+ P WLE +  S Y P      R +   +V++
Sbjct: 645 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 697
>sp|Q6GWX0|DDX4_PIG DEAD-box protein 4 (VASA homolog) (VASA-like protein)
          Length = 722

 Score = 31.6 bits (70), Expect = 0.58
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157
           D + D  LA   VK+L DA Q+ P WLE +  S Y P      R +   +V++
Sbjct: 643 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 695
>sp|Q4R5S7|DDX4_MACFA DEAD-box protein 4
          Length = 725

 Score = 31.6 bits (70), Expect = 0.58
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLC-SGYRPSEHNRNRRDYRKNVES 157
           D + D  LA   VK+L DA Q+ P WLE +  S Y P      R +   +V++
Sbjct: 646 DLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDT 698
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11)
          Length = 661

 Score = 31.2 bits (69), Expect = 0.76
 Identities = 16/42 (38%), Positives = 25/42 (59%)
 Frame = +2

Query: 2   DEDKDVALAPRFVKILKDAGQECPNWLESLCSGYRPSEHNRN 127
           D +KD A+A   VKIL+ +GQ  P++L +  +G      N+N
Sbjct: 594 DPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQN 635
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2)
           (mDEAD2) (D1Pas1-related sequence 1)
          Length = 658

 Score = 28.9 bits (63), Expect = 3.8
 Identities = 17/62 (27%), Positives = 33/62 (53%), Gaps = 11/62 (17%)
 Frame = +2

Query: 8   DKDVALAPRFVKILKDAGQECPNWLESLC--SGYRPSEHNRNR---------RDYRKNVE 154
           ++++ +    + +L +A QE P+WLES+     Y+ S   R++         RDYR++  
Sbjct: 546 ERNLNITKDLLDLLVEAKQEVPSWLESMAYEHHYKGSSRGRSKSRFSGGFGARDYRQSSG 605

Query: 155 SS 160
           S+
Sbjct: 606 SA 607
>sp|P16042|NEU4_ONCKE Vasotocin-neurophysin VT 2 precursor [Contains: Vasotocin (VT);
           Neurophysin VT 2]
          Length = 158

 Score = 28.5 bits (62), Expect = 4.9
 Identities = 13/28 (46%), Positives = 14/28 (50%), Gaps = 1/28 (3%)
 Frame = +1

Query: 10  QGCCISSKVC-KNS*GCWTGMPELAREF 90
           QG C    +C     GCW G PE AR F
Sbjct: 49  QGHCFGPSICCGEGLGCWMGSPETARCF 76
>sp|Q9JIB4|TF2H2_MOUSE TFIIH basal transcription factor complex p44 subunit (Basic
           transcription factor 2 44 kDa subunit) (BTF2-p44)
           (General transcription factor IIH polypeptide 2)
          Length = 396

 Score = 28.1 bits (61), Expect = 6.5
 Identities = 10/32 (31%), Positives = 21/32 (65%)
 Frame = -1

Query: 212 IKKNHHHLRAQIYFQQMLTIQHFYGSPFCFGC 117
           + +++HHL     FQ+ ++++ + G  FC+GC
Sbjct: 319 LARSYHHLFPLDAFQE-ISLEEYKGERFCYGC 349
>sp|Q9CM18|SPRT_PASMU Protein sprT
          Length = 165

 Score = 27.7 bits (60), Expect = 8.4
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = +3

Query: 135 TTVKMLNRQHLLKINLCTQMMMIFFNRSFDSMKNH 239
           T ++ LNRQ   K+  C Q+   +FNR+F     H
Sbjct: 5   TQLRHLNRQVQRKLTQCLQLASGYFNRTFSMPTVH 39
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,088,034
Number of Sequences: 369166
Number of extensions: 648879
Number of successful extensions: 1562
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1562
length of database: 68,354,980
effective HSP length: 86
effective length of database: 52,467,770
effective search space used: 1678968640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)