Planarian EST Database


Dr_sW_013_E06-1

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_013_E06-1
         (489 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q62217|SEM5A_MOUSE  Semaphorin-5A precursor (Semaphorin F...    30   2.3  
sp|Q7ZZV4|TSHB_ANGJA  Thyrotropin beta chain precursor (Thyr...    30   2.3  
sp|P98133|FBN1_BOVIN  Fibrillin-1 precursor (MP340)                30   3.0  
sp|Q92HI8|PPDK_RICCN  Pyruvate, phosphate dikinase (Pyruvate...    30   3.0  
sp|Q08127|TSHB_ANGAN  Thyrotropin beta chain precursor (Thyr...    30   3.9  
sp|P35555|FBN1_HUMAN  Fibrillin-1 precursor                        29   5.1  
sp|Q09272|YQG1_CAEEL  Hypothetical protein C41C4.1 in chromo...    29   5.1  
sp|P10820|PERF_MOUSE  Perforin 1 precursor (P1) (Lymphocyte ...    29   6.7  
sp|Q01253|YSCL_YEREN  Yop proteins translocation protein L         29   6.7  
sp|Q13591|SEM5A_HUMAN  Semaphorin-5A precursor (Semaphorin F...    29   6.7  
>sp|Q62217|SEM5A_MOUSE Semaphorin-5A precursor (Semaphorin F) (Sema F)
          Length = 1077

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 30/124 (24%), Positives = 47/124 (37%), Gaps = 18/124 (14%)
 Frame = -2

Query: 389  RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 210
            R R C   +P   QC      +  C FD   I  VS A S   +++ C   N+       
Sbjct: 916  RARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMFHMM 975

Query: 209  XQG-----FGCINCVLNFQNC-----------CVSPINP--LNNYIAQNFCNFIDDQQ*L 84
              G      GC+  +L +  C            + P++P  LN+ I  N  N +D    +
Sbjct: 976  AVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSIT-NHINKLDKYDSV 1034

Query: 83   DFLK 72
            + +K
Sbjct: 1035 EAIK 1038
>sp|Q7ZZV4|TSHB_ANGJA Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
           subunit) (TSH-beta) (TSH-B)
          Length = 147

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
 Frame = -2

Query: 365 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 240
           Y ++ +C+ CV  N   C       DP   N+V PA+     QR CT+
Sbjct: 30  YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLAVQRGCTY 74
>sp|P98133|FBN1_BOVIN Fibrillin-1 precursor (MP340)
          Length = 2871

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
 Frame = -2

Query: 467  ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 315
            AC++I+E +L        +C N        C+  Y   PDR  C   + C   N  C   
Sbjct: 1152 ACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211

Query: 314  CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXQGFGCINCVLNFQNCC 159
            C +       S  P  ++   QRSCT I+          G  C N    ++  C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265
>sp|Q92HI8|PPDK_RICCN Pyruvate, phosphate dikinase (Pyruvate, orthophosphate dikinase)
          Length = 882

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
 Frame = -3

Query: 463 VWKSVKRNCQKLKIDFNNAQTIQCLVADGVLKLIQTDFNVKIAFKLITIAV-LIHETLIL 287
           V+K + +  QKL+  + + Q I+  + +  L ++QT    + A   I IAV ++ E LI 
Sbjct: 312 VFKELSQIAQKLEEHYLDMQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLIS 371

Query: 286 CHQLCQYVDNKEAVLLL 236
             Q    +D +    LL
Sbjct: 372 KEQALMRIDPESLNQLL 388
>sp|Q08127|TSHB_ANGAN Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
           subunit) (TSH-beta) (TSH-B)
          Length = 147

 Score = 29.6 bits (65), Expect = 3.9
 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
 Frame = -2

Query: 365 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 240
           Y ++ +C+ CV  N   C       DP   N+V PA+     QR CT+
Sbjct: 30  YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLVVQRGCTY 74
>sp|P35555|FBN1_HUMAN Fibrillin-1 precursor
          Length = 2871

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
 Frame = -2

Query: 467  ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 315
            AC++I+E +L        +C N        C+  Y   PDR  C   + C   N  C   
Sbjct: 1152 ACIDINECELSAHLCPNGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211

Query: 314  CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXQGFGCINCVLNFQNCC 159
            C +       S  P  ++   QRSCT I+          G  C N    ++  C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265
>sp|Q09272|YQG1_CAEEL Hypothetical protein C41C4.1 in chromosome II
          Length = 329

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -2

Query: 404 NYSMSRCRWCSQAYPDRFQCENCVQTNNNC 315
           ++  ++C + +Q YPDR QC NC Q N  C
Sbjct: 165 SFFSNKCIFEAQKYPDRKQCYNC-QGNVTC 193
>sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte pore forming protein)
           (Cytolysin)
          Length = 554

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 12/58 (20%)
 Frame = -2

Query: 449 ETQLPETQNRFQQCPNYSMSRCRWCSQAYPDR------------FQCENCVQTNNNCC 312
           E Q P+ +   Q   +Y MSR RW + + P R             +C++   TN +CC
Sbjct: 350 EEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCC 407
>sp|Q01253|YSCL_YEREN Yop proteins translocation protein L
          Length = 223

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
 Frame = -3

Query: 316 AVLIHETLILCHQLCQYVDNK--EAVLLLTCHNVKDVDNRDL 197
           A LIHET + C Q  ++V+ +  E VLL     + D D  D+
Sbjct: 85  ATLIHETQLQCQQFYRHVEQQMSEVVLLAVRKILNDYDQVDM 126
>sp|Q13591|SEM5A_HUMAN Semaphorin-5A precursor (Semaphorin F) (Sema F)
          Length = 1074

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 29/121 (23%), Positives = 45/121 (37%), Gaps = 15/121 (12%)
 Frame = -2

Query: 389  RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 210
            R R C   +P   QC      +  C FD   I  VS A S   +++ C   N+       
Sbjct: 916  RARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVG 975

Query: 209  XQG--FGCINCVLNFQNC-----------CVSPIN--PLNNYIAQNFCNFIDDQQ*LDFL 75
                  GC+  +L +  C            + P++  PLN  I  N  N +D    ++ +
Sbjct: 976  LSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSIT-NHINKLDKYDSVEAI 1034

Query: 74   K 72
            K
Sbjct: 1035 K 1035
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,935,419
Number of Sequences: 369166
Number of extensions: 985151
Number of successful extensions: 2218
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2217
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2970720600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)