Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_C07
(781 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9U4L6|TOM40_DROME Probable mitochondrial import recepto... 174 2e-43
sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import recepto... 139 1e-32
sp|O96008|TOM40_HUMAN Probable mitochondrial import recepto... 135 2e-31
sp|Q18090|TOM40_CAEEL Probable mitochondrial import recepto... 134 3e-31
sp|O13656|TOM40_SCHPO Probable mitochondrial import recepto... 59 1e-08
sp|Q59088|G6PI_ACIAD Glucose-6-phosphate isomerase (GPI) (P... 32 1.9
sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component ... 31 3.3
sp|O74161|CHS5_CANAL Chitin biosynthesis protein CHS5 31 3.3
sp|Q9RMC1|G6PI_ACILW Glucose-6-phosphate isomerase (GPI) (P... 30 9.7
sp|P27571|XIST_MOUSE X inactive-specific transcript protein 30 9.7
>sp|Q9U4L6|TOM40_DROME Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Male sterile protein 15)
Length = 344
Score = 174 bits (441), Expect = 2e-43
Identities = 86/242 (35%), Positives = 140/242 (57%), Gaps = 2/242 (0%)
Frame = +1
Query: 4 SGYKFGATYVGNTKLSETEIYPIMLGELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKID 183
SGY+FGATYVG + S TE +P++LG++ P GNLNA +IHQF+ +++CK+ SQ++ K+
Sbjct: 107 SGYRFGATYVGTKEFSPTEAFPVLLGDIDPAGNLNANVIHQFSARLRCKFASQIQESKVV 166
Query: 184 GQQFSIEHRNKNINLGFSIVNPSIQNKEISCIFDVMRRITDKLSLGSMFVFQRSNQAQFT 363
Q + ++R + L ++ NPSI + ++ +T L+LGS +Q
Sbjct: 167 ASQLTTDYRGSDYTLSLTVANPSIFTNSGVVVGQYLQSVTPALALGSELAYQFGPNVPGR 226
Query: 364 NFGVWNVGGQYVGNK--WTAAVGVRPFQSSLHSTFHMKLNEAFQIGTEIETSLGHRQCVG 537
+ +V G+Y W+ +G QS LH ++ K ++ QIG E+ETSL ++ V
Sbjct: 227 QIAIMSVVGRYTAGSSVWSGTLG----QSGLHVCYYQKASDQLQIGAEVETSLRMQESVA 282
Query: 538 TAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGF 717
T YQ D PK+ + + +DSNW+I E++ +AP+PF++ L + K+N+ LG G
Sbjct: 283 TLAYQIDLPKANLVFRGGIDSNWQIFGVLEKR-LAPLPFTLALSGRMNHVKNNFRLGCGL 341
Query: 718 SI 723
I
Sbjct: 342 MI 343
>sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 359
Score = 139 bits (349), Expect = 1e-32
Identities = 75/241 (31%), Positives = 129/241 (53%)
Frame = +1
Query: 1 QSGYKFGATYVGNTKLSETEIYPIMLGELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKI 180
+S Y FG TYVG +LS TE +P+++G++ +G+LNAQ+IHQ + ++ K Q + K
Sbjct: 124 ESNYHFGVTYVGTKQLSPTEAFPVLVGDMDNSGSLNAQVIHQLSPGLRSKMAIQTQQSKF 183
Query: 181 DGQQFSIEHRNKNINLGFSIVNPSIQNKEISCIFDVMRRITDKLSLGSMFVFQRSNQAQF 360
Q E+R + ++ NP + + ++ IT L+LG V+ R +
Sbjct: 184 VNWQVDGEYRGSDFTAAVTLGNPDVLVGSGILVAHYLQSITPCLALGGELVYHRRPGEEG 243
Query: 361 TNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLNEAFQIGTEIETSLGHRQCVGT 540
T V ++ G+Y N W A V + Q+ +H+T++ K ++ Q+G E E S + +
Sbjct: 244 T---VMSLAGKYTLNNWLATVTLG--QAGMHATYYHKASDQLQVGVEFEASTRMQDTSAS 298
Query: 541 AGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGFS 720
GYQ D PK+ K V+SNW + + E+K + P+P +++LC + K+ + G G +
Sbjct: 299 FGYQLDLPKANFLFKGSVNSNWIVGATLEKK-LPPLPLTLSLCAFLNHRKNKFLCGFGLT 357
Query: 721 I 723
I
Sbjct: 358 I 358
>sp|O96008|TOM40_HUMAN Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Haymaker protein) (p38.5)
Length = 361
Score = 135 bits (339), Expect = 2e-31
Identities = 75/241 (31%), Positives = 127/241 (52%)
Frame = +1
Query: 1 QSGYKFGATYVGNTKLSETEIYPIMLGELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKI 180
+S Y FG TYVG +LS TE +P+++G++ +G+LNAQ+IHQ ++ K Q + K
Sbjct: 126 ESNYHFGVTYVGTKQLSPTEAFPVLVGDMDNSGSLNAQVIHQLGPGLRSKMAIQTQQSKF 185
Query: 181 DGQQFSIEHRNKNINLGFSIVNPSIQNKEISCIFDVMRRITDKLSLGSMFVFQRSNQAQF 360
Q E+R + ++ NP + + ++ IT L+LG V+ R +
Sbjct: 186 VNWQVDGEYRGSDFTAAVTLGNPDVLVGSGILVAHYLQSITPCLALGGELVYHRRPGEEG 245
Query: 361 TNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLNEAFQIGTEIETSLGHRQCVGT 540
T V ++ G+Y N W A V + Q+ +H+T++ K ++ Q+G E E S + +
Sbjct: 246 T---VMSLAGKYTLNNWLATVTLG--QAGMHATYYHKASDQLQVGVEFEASTRMQDTSVS 300
Query: 541 AGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGFS 720
GYQ D PK+ + K VDSNW + + E+K + P+P ++ L + K+ + G G +
Sbjct: 301 FGYQLDLPKANLLFKGSVDSNWIVGATLEKK-LPPLPLTLALGAFLNHRKNKFQCGFGLT 359
Query: 721 I 723
I
Sbjct: 360 I 360
>sp|Q18090|TOM40_CAEEL Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 301
Score = 134 bits (337), Expect = 3e-31
Identities = 73/240 (30%), Positives = 124/240 (51%)
Frame = +1
Query: 4 SGYKFGATYVGNTKLSETEIYPIMLGELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKID 183
+GY+FGATYVG ++ E YPI+LG+ NGN A I+HQ + K Q++ GK+
Sbjct: 64 TGYRFGATYVGTNQVGPAEAYPILLGDTDVNGNTTATILHQLGI-YRTKLQGQIQQGKLA 122
Query: 184 GQQFSIEHRNKNINLGFSIVNPSIQNKEISCIFDVMRRITDKLSLGSMFVFQRSNQAQFT 363
G Q +IE + + LG ++ N + N+ + +RR+T +L +G+ V+Q
Sbjct: 123 GAQATIERKGRLSTLGLTLANIDLVNEAGILVGQFLRRLTPRLDVGTEMVYQYGKNIPGG 182
Query: 364 NFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLNEAFQIGTEIETSLGHRQCVGTA 543
V + +Y N + AA + S +H T++ K NE G E E + + V T
Sbjct: 183 QISVLSYAARYTANHFIAAATLGA--SGVHLTYYHKQNENLAFGVEFECNANVGEAVTTL 240
Query: 544 GYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGFSI 723
YQ + P+ G+T+++ D+NW + +E++ +PF++ L + K+ G+G I
Sbjct: 241 AYQTELPEEGVTMRASFDTNWTVGGVFEKRLSQQLPFTLALSGTLNHVKAAGKFGIGLII 300
>sp|O13656|TOM40_SCHPO Probable mitochondrial import receptor subunit tom40 (Translocase
of outer membrane 40 kDa subunit)
Length = 344
Score = 59.3 bits (142), Expect = 1e-08
Identities = 51/234 (21%), Positives = 99/234 (42%), Gaps = 15/234 (6%)
Frame = +1
Query: 67 PIML-GELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKIDGQ-QFSIEHRNKNINLGFSI 240
P+ L G + +G + A + +N + K Q+ G + Q +H+ K+ + F
Sbjct: 94 PLFLRGSVDNDGAVQAMLNCTWNSNVLSKVQMQLSNGAVPNMCQIEHDHKGKDFSFSFKA 153
Query: 241 VNPSIQNKEISC-IFDVMRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTA 417
+NP + K I +++ +T KLSLG ++Q+ + + + +Y W A
Sbjct: 154 MNPWYEEKLTGIYIISLLQSVTPKLSLGVEALWQKPSSSIGPEEATLSYMTRYNAADWIA 213
Query: 418 AVGVRPFQSSLHSTFHMKLNEAFQIGTEIETS---LGHRQCVGT---------AGYQFDF 561
+ Q + +TF KL+ + G E + S L H + T G +++F
Sbjct: 214 TAHLNGSQGDVTATFWRKLSPKVEAGVECQLSPVGLNHSAALMTGPKPEGLTSVGVKYEF 273
Query: 562 PKSGITIKSQVDSNWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGFSI 723
+S + QVDS ++ ER+ I + + ++ + GLG+ +
Sbjct: 274 AQS--IYRGQVDSKGRVGVYLERRLAPAITLAFSSELDHPNRNAKVGLGLSLEL 325
>sp|Q59088|G6PI_ACIAD Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
Length = 557
Score = 32.0 bits (71), Expect = 1.9
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +1
Query: 37 NTKLSETEIYPIMLGELSPNG-NLNAQIIHQFNQKIKCKYVSQM 165
N + E PI+ GE+ PN + Q++HQ QK+ C +++ M
Sbjct: 370 NGQKVENTTCPIVWGEVGPNAQHAFYQLLHQGTQKVSCDFIAPM 413
>sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component N-recognin-1 (N-end-recognizing
protein)
Length = 1950
Score = 31.2 bits (69), Expect = 3.3
Identities = 17/71 (23%), Positives = 35/71 (49%)
Frame = -2
Query: 573 TRFREIKLITSCTHALTMTQTSLNFCTYLKCFIQLHVKS*M*RTLERPNSHCRSPFITHI 394
T+ +EIKL + + L+F L C +++H+++ + N +C PF +
Sbjct: 1676 TQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKNCFKPFGAFL 1735
Query: 393 LPTNIPYTEIC 361
+P + +E+C
Sbjct: 1736 MPNS---SEVC 1743
>sp|O74161|CHS5_CANAL Chitin biosynthesis protein CHS5
Length = 562
Score = 31.2 bits (69), Expect = 3.3
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +1
Query: 43 KLSETEIYPIMLGELSPNGNLNAQIIHQFNQKIKCKYVSQMKVGKIDGQQFSIEHRNK 216
K+++ + LG+L+PN N + I + + + KY Q +V K+D F NK
Sbjct: 165 KMTDLSGITVCLGDLTPNDQFNKEDIEEALKNMGAKYPVQQQV-KVDTTHFLCTRENK 221
>sp|Q9RMC1|G6PI_ACILW Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
Length = 557
Score = 29.6 bits (65), Expect = 9.7
Identities = 12/35 (34%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +1
Query: 67 PIMLGELSPNG-NLNAQIIHQFNQKIKCKYVSQMK 168
PI+ GE+ PN + Q++HQ K+ C +++ +K
Sbjct: 380 PIVWGEVGPNAQHAFYQLLHQGTHKVSCDFIAPVK 414
>sp|P27571|XIST_MOUSE X inactive-specific transcript protein
Length = 298
Score = 29.6 bits (65), Expect = 9.7
Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = -2
Query: 651 WYRSYKLSLVRCSDFPITINLRFDCNTRFREIKLITSCTH-ALTMTQTSLNFCTYLKC 481
W L++ C + + I+L F FR+ +T C H +L + T L C+ C
Sbjct: 129 WSALSSLAMALCFIYLVAISLSFSFTPAFRDSLWVTCCMHPSLFLVLTPLVLCSSSLC 186
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,844,401
Number of Sequences: 369166
Number of extensions: 1946665
Number of successful extensions: 4152
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4020
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4141
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7308943600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)