Planarian EST Database


Dr_sW_013_C04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_013_C04
         (768 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q96B97|SH3K1_HUMAN  SH3-domain kinase binding protein 1 (...    33   0.65 
sp|Q07157|ZO1_HUMAN  Tight junction protein ZO-1 (Zonula occ...    33   1.1  
sp|P08297|NO75_SOYBN  Early nodulin 75 precursor (N-75) (NGM...    32   1.9  
sp|P39447|ZO1_MOUSE  Tight junction protein ZO-1 (Zonula occ...    32   1.9  
sp|Q64028|PHC1_MOUSE  Polyhomeotic-like protein 1 (Early dev...    32   2.5  
sp|Q8R550|SH3K1_MOUSE  SH3-domain kinase binding protein 1 (...    32   2.5  
sp|Q925Q9|SH3K1_RAT  SH3-domain kinase binding protein 1 (SH...    32   2.5  
sp|P25558|BUD3_YEAST  Bud site selection protein BUD3              31   3.2  
sp|Q9PGD4|PBPA_XYLFA  Penicillin-binding protein 1A (PBP-1a)...    30   5.5  
sp|P16329|NO75_PEA  Early nodulin 75 protein (N-75) (NGM-75)       30   7.2  
>sp|Q96B97|SH3K1_HUMAN SH3-domain kinase binding protein 1 (Cbl-interacting protein of 85
           kDa) (Human Src-family kinase binding protein 1) (HSB-1)
           (CD2 binding protein 3) (CD2BP3)
          Length = 665

 Score = 33.5 bits (75), Expect = 0.65
 Identities = 28/77 (36%), Positives = 38/77 (49%), Gaps = 3/77 (3%)
 Frame = +3

Query: 405 PLMKVNQQSEPEIIKKPENNAHL---KQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQT 575
           P M  N+  E E   +PE    L   K ++ A  PK+ +P    PKT     ++SRP   
Sbjct: 369 PEMLPNRTEEKE---RPEREPKLDLQKPSVPAIPPKKPRP----PKT----NSLSRPG-A 416

Query: 576 LPKKEPTRPVGPACLTK 626
           LP + P RPVGP   T+
Sbjct: 417 LPPRRPERPVGPLTHTR 433
>sp|Q07157|ZO1_HUMAN Tight junction protein ZO-1 (Zonula occludens 1 protein) (Zona
            occludens 1 protein) (Tight junction protein 1)
          Length = 1748

 Score = 32.7 bits (73), Expect = 1.1
 Identities = 14/42 (33%), Positives = 27/42 (64%)
 Frame = +3

Query: 447  KKPENNAHLKQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQ 572
            + P+ N +L  + TA+KP  +  T   PKT+ K+ ++++PP+
Sbjct: 1544 ESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPE 1585
>sp|P08297|NO75_SOYBN Early nodulin 75 precursor (N-75) (NGM-75)
          Length = 309

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 20/76 (26%), Positives = 29/76 (38%)
 Frame = +3

Query: 405 PLMKVNQQSEPEIIKKPENNAHLKQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQTLPK 584
           P  K  +   P     PE     ++    Y+P Q +P +  P   Y+  +   PP+  P 
Sbjct: 184 PHEKPPEHQPPHEKPPPEYQPPHEKPPPEYQPPQEKPPHEKPPPEYQPPHEKPPPEHQPP 243

Query: 585 KEPTRPVGPACLTKKP 632
            E   PV P    K P
Sbjct: 244 HEKPPPVYPPPYEKPP 259
>sp|P39447|ZO1_MOUSE Tight junction protein ZO-1 (Zonula occludens 1 protein) (Zona
            occludens 1 protein) (Tight junction protein 1)
          Length = 1745

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 14/42 (33%), Positives = 26/42 (61%)
 Frame = +3

Query: 447  KKPENNAHLKQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQ 572
            + P+ N +L  + T +KP+ +  T   PKT+ KA + ++PP+
Sbjct: 1541 ESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPE 1582
>sp|Q64028|PHC1_MOUSE Polyhomeotic-like protein 1 (Early development regulator protein 1)
           (MPH1) (RAE-28)
          Length = 1012

 Score = 31.6 bits (70), Expect = 2.5
 Identities = 23/114 (20%), Positives = 44/114 (38%), Gaps = 8/114 (7%)
 Frame = +3

Query: 315 QREIVLSHMKNLNELQXXXXXXXXXXXXXXPLMKVNQQSEPEIIKKPENNAHLKQTMTAY 494
           Q++I + H +     Q                 +  QQ + +  ++ +       T+TA 
Sbjct: 396 QQQIAIHHQQQFQHRQSQLLHTATHLQLAQQQQQQQQQQQQQQQQQQQQQQQQGTTLTAP 455

Query: 495 KPKQAQPTNRIP--------KTVYKAKNVSRPPQTLPKKEPTRPVGPACLTKKP 632
           +P Q  PT ++P        +T+     +   P TLP +  ++P  P  +  KP
Sbjct: 456 QPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLTLPPEPTSKP--PIPIQSKP 507
>sp|Q8R550|SH3K1_MOUSE SH3-domain kinase binding protein 1 (SH3 containing, expressed in
           tumorigenic astrocytes) (Regulator of ubiquitous kinase)
           (Ruk)
          Length = 709

 Score = 31.6 bits (70), Expect = 2.5
 Identities = 22/63 (34%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
 Frame = +3

Query: 447 KKPENNAHL---KQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQTLPKKEPTRPVGPAC 617
           ++PE    L   K ++ A  PK+ +P    PKT     N    P  LP + P RPVGP  
Sbjct: 424 ERPEREPKLDLQKPSVPAIPPKKPRP----PKT-----NSLNRPGALPPRRPERPVGPLT 474

Query: 618 LTK 626
            T+
Sbjct: 475 HTR 477
>sp|Q925Q9|SH3K1_RAT SH3-domain kinase binding protein 1 (SH3 containing, expressed in
           tumorigenic astrocytes) (Regulator of ubiquitous kinase)
           (Ruk)
          Length = 709

 Score = 31.6 bits (70), Expect = 2.5
 Identities = 22/63 (34%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
 Frame = +3

Query: 447 KKPENNAHL---KQTMTAYKPKQAQPTNRIPKTVYKAKNVSRPPQTLPKKEPTRPVGPAC 617
           ++PE    L   K ++ A  PK+ +P    PKT     N    P  LP + P RPVGP  
Sbjct: 424 ERPEREPKLDLQKPSVPAIPPKKPRP----PKT-----NSLNRPGVLPPRRPERPVGPLT 474

Query: 618 LTK 626
            T+
Sbjct: 475 HTR 477
>sp|P25558|BUD3_YEAST Bud site selection protein BUD3
          Length = 1636

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 23/87 (26%), Positives = 41/87 (47%), Gaps = 7/87 (8%)
 Frame = +3

Query: 414  KVNQQSEPEIIKKPENNAHLKQTMTAYKPKQAQPTNRIPKTVYKAKNVSRP------PQT 575
            K + +   EI KK + NA   + +   KP + +    + K V+ +K+ S+P      P+ 
Sbjct: 816  KKSSKKpreIQKKTKTNASKAEHIEKKKPNKGKGFFGVLKNVFGSKSKSKPSPVQRVPKK 875

Query: 576  LPKKEPTRPV-GPACLTKKPNNVRIVI 653
            + ++ P  PV  P    KK +  R V+
Sbjct: 876  ISQRHPKSPVKKPMTSEKKSSPKRAVV 902
>sp|Q9PGD4|PBPA_XYLFA Penicillin-binding protein 1A (PBP-1a) (PBP1a) [Includes:
           Penicillin-insensitive transglycosylase (Peptidoglycan
           TGase); Penicillin-sensitive transpeptidase
           (DD-transpeptidase)]
          Length = 809

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +3

Query: 549 KNVSRPPQTLPKKEPTRPVGPACLTKK 629
           KN + PPQT+P  +P  P+ P  + ++
Sbjct: 642 KNNNEPPQTIPSPDPNTPMAPRAIDER 668
>sp|P16329|NO75_PEA Early nodulin 75 protein (N-75) (NGM-75)
          Length = 112

 Score = 30.0 bits (66), Expect = 7.2
 Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 9/49 (18%)
 Frame = +3

Query: 492 YKPKQAQPTNRIPKTVYKAKNVSRPP---------QTLPKKEPTRPVGP 611
           Y+P   +P +  P  VYK    + PP         Q  P  +P+RP GP
Sbjct: 58  YQPPHEKPPHENPPPVYKPPYENSPPPHVYHRPLFQAPPPVKPSRPFGP 106
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,845,852
Number of Sequences: 369166
Number of extensions: 899291
Number of successful extensions: 2701
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2685
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7115329200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)