Planarian EST Database


Dr_sW_013_B13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_013_B13
         (671 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O32617|FTSH_HELFE  Cell division protein ftsH homolog           32   1.1  
sp|P08799|MYS2_DICDI  Myosin II heavy chain, non muscle            32   2.0  
sp|Q8BWJ3|KPB2_MOUSE  Phosphorylase b kinase alpha regulator...    32   2.0  
sp|Q8WT54|FAR1_WUCBA  Fatty-acid and retinol-binding protein...    32   2.0  
sp|Q962W7|FAR1_LOALO  Fatty-acid and retinol-binding protein...    32   2.0  
sp|P18106|FPS_DROME  Tyrosine-protein kinase Fps85D (dFer)         31   2.6  
sp|P41993|YKC2_CAEEL  Hypothetical protein B0280.2 in chromo...    31   3.3  
sp|Q8A455|SYE_BACTN  Glutamyl-tRNA synthetase (Glutamate--tR...    31   3.3  
sp|Q8BKE9|IFT74_MOUSE  Intraflagellar transport 74 homolog (...    31   3.3  
sp|P12753|RAD50_YEAST  DNA repair protein RAD50 (153 kDa pro...    30   4.4  
>sp|O32617|FTSH_HELFE Cell division protein ftsH homolog
          Length = 638

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 23/81 (28%), Positives = 41/81 (50%), Gaps = 7/81 (8%)
 Frame = +2

Query: 143 ISEEKRNR---SDFESKSDWQERIALE----VKNFLRPAYMSKKITKSDYKEIMKRCVTK 301
           + E++RN      F S  ++ E++A E    +KN L   Y+  K T SDYK+ ++  V +
Sbjct: 542 VLEKQRNSFLGGGFGSGREFSEKMAEEMDSFIKNLLEERYVHVKQTLSDYKDAIEVMVNE 601

Query: 302 ISRSGVTEINTEKIEKFVLSY 364
           +    V  I  E++ + +  Y
Sbjct: 602 LFEKEV--ITGERVREIISEY 620
>sp|P08799|MYS2_DICDI Myosin II heavy chain, non muscle
          Length = 2116

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 28/102 (27%), Positives = 46/102 (45%), Gaps = 4/102 (3%)
 Frame = +2

Query: 86   SNTAQTNQS-SKQLSSTSNEISEEKRNRSDFESKSDWQERIALEVKNFLRPAYMSKKI-- 256
            SN  ++ ++   QL + +NE+ EEK+NR   E K    + +  E+K+ L      KK   
Sbjct: 1083 SNVEKSKKTLESQLVAVNNELDEEKKNRDALEKKKKALDAMLEEMKDQLESTGGEKKSLY 1142

Query: 257  -TKSDYKEIMKRCVTKISRSGVTEINTEKIEKFVLSYVDKYQ 379
              K   +  M+    +IS    T    EKI+  +   V + Q
Sbjct: 1143 DLKVKQESDMEALRNQISELQSTIAKLEKIKSTLEGEVARLQ 1184
>sp|Q8BWJ3|KPB2_MOUSE Phosphorylase b kinase alpha regulatory chain, liver isoform
            (Phosphorylase kinase alpha L subunit)
          Length = 1235

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 21/78 (26%), Positives = 35/78 (44%), Gaps = 3/78 (3%)
 Frame = +2

Query: 113  SKQLSSTSNEISEEKRNRSDFESKSDWQERIA---LEVKNFLRPAYMSKKITKSDYKEIM 283
            ++ L+ +  E SE   N S F+ KS     ++     V+  +RP + S         E+ 
Sbjct: 929  ARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVG 988

Query: 284  KRCVTKISRSGVTEINTE 337
                TK  RSG+T + +E
Sbjct: 989  HTGATKTERSGITRLRSE 1006
>sp|Q8WT54|FAR1_WUCBA Fatty-acid and retinol-binding protein 1 precursor (Wb-FAR-1)
          Length = 178

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 25/97 (25%), Positives = 49/97 (50%), Gaps = 1/97 (1%)
 Frame = +2

Query: 92  TAQTNQSSKQLSSTSNEISEEKRNRSDFESKSDWQERIALEVKNFLRPAYMS-KKITKSD 268
           T +  +  ++L+S     + E       ++KSD   + A+E++NF++    S K   K+ 
Sbjct: 46  TVEDKEILRELASKHATFANEDAALEALKAKSDNLYKNAVELRNFVKAKIDSLKPDAKTF 105

Query: 269 YKEIMKRCVTKISRSGVTEINTEKIEKFVLSYVDKYQ 379
             EI+ +  +  S  G  +++TEKI++     + KYQ
Sbjct: 106 VDEIIAKARSLRSDDG-HKLDTEKIKQAARDIIAKYQ 141
>sp|Q962W7|FAR1_LOALO Fatty-acid and retinol-binding protein 1 precursor (Ll-FAR-1)
           (Ll20)
          Length = 178

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 25/97 (25%), Positives = 47/97 (48%), Gaps = 1/97 (1%)
 Frame = +2

Query: 92  TAQTNQSSKQLSSTSNEISEEKRNRSDFESKSDWQERIALEVKNFLRPAYMS-KKITKSD 268
           TA+  +  + L+S     + E       + KSD   + A+E++NF++    S K   K+ 
Sbjct: 46  TAEDKEILRDLASKHATFANEDAALEALKDKSDKLYKNAVELRNFVKAKIDSLKPDAKAF 105

Query: 269 YKEIMKRCVTKISRSGVTEINTEKIEKFVLSYVDKYQ 379
             E++ R  +  S  G  + +T+KI++     + KYQ
Sbjct: 106 VDEVIARARSLRSDDG-QKFDTDKIKQAARDIIAKYQ 141
>sp|P18106|FPS_DROME Tyrosine-protein kinase Fps85D (dFer)
          Length = 1325

 Score = 31.2 bits (69), Expect = 2.6
 Identities = 22/62 (35%), Positives = 27/62 (43%)
 Frame = +2

Query: 176 ESKSDWQERIALEVKNFLRPAYMSKKITKSDYKEIMKRCVTKISRSGVTEINTEKIEKFV 355
           E +   QE   L  +N L+ A     +T   YKEI KR  T I     TE   E  EK+ 
Sbjct: 214 EHQQSVQESFILLWRNILQEAAQYGDLTADKYKEIQKRIDTVIGSINPTEEYGEFTEKYK 273

Query: 356 LS 361
            S
Sbjct: 274 TS 275
>sp|P41993|YKC2_CAEEL Hypothetical protein B0280.2 in chromosome III
          Length = 627

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 24/120 (20%), Positives = 57/120 (47%), Gaps = 8/120 (6%)
 Frame = +2

Query: 32  LASSAAAIMANVSM--PLPMSNTAQTNQSSKQLSSTSNEISEEKRNRSDFESKSDWQERI 205
           L+ S+ ++  +++M  P P+  T++   +S  L  + +EI+          SKS+ Q  +
Sbjct: 502 LSDSSTSVAQDIAMKVPTPLPRTSKIISASSPLPPSQDEINPLIEMVDQSSSKSETQIPV 561

Query: 206 ALEVKNFLRPAYMSKKITKSDYKEIMKRCVT------KISRSGVTEINTEKIEKFVLSYV 367
               ++ L     + +I  +D+ +++ + ++      K  +S   E+    IEK + +Y+
Sbjct: 562 TECSQSHLNGKGSAAQIQSADFDKVLNQLLSIKVNSEKSKQSADLELLLVSIEKLIQNYL 621
>sp|Q8A455|SYE_BACTN Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
          Length = 504

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 14/50 (28%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
 Frame = +2

Query: 224 FLRPAYMSKKITKSDYKEIMKRCVTKISR--SGVTEINTEKIEKFVLSYV 367
           F+ PA   +K  K  +KE   +C+T+++   +G+ + + E  EK V+ ++
Sbjct: 404 FVAPAEYDEKTVKKRWKEDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWI 453
>sp|Q8BKE9|IFT74_MOUSE Intraflagellar transport 74 homolog (Coiled-coil domain-containing
           protein 2) (Capillary morphogenesis protein 1) (CMG-1)
          Length = 600

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 29/114 (25%), Positives = 50/114 (43%), Gaps = 7/114 (6%)
 Frame = +2

Query: 2   LPNYSDVLKPLASSAAAIMANVSMPLPMSNTAQTNQSSKQLSSTSNEISEEKRNRSDFES 181
           L  YSD L  L SSA      +     + +T + N   K +   +++    KR   D E+
Sbjct: 481 LETYSD-LAALKSSAEEKKKKLHQERTVLSTHR-NAFKKIMEKLTSDYDTLKRQLQDNET 538

Query: 182 KSD-------WQERIALEVKNFLRPAYMSKKITKSDYKEIMKRCVTKISRSGVT 322
            +        WQ    LE  NF+   +++ K  +SDY+ ++K  + +I+    T
Sbjct: 539 HAQLTNLERKWQH---LEQNNFVMKEFIATKSQESDYQPVIKNVMKQIAEYNKT 589
>sp|P12753|RAD50_YEAST DNA repair protein RAD50 (153 kDa protein)
          Length = 1312

 Score = 30.4 bits (67), Expect = 4.4
 Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 2/62 (3%)
 Frame = +2

Query: 89   NTAQTNQSSKQLSSTSNEISEEKRNRSDF--ESKSDWQERIALEVKNFLRPAYMSKKITK 262
            N AQ  +  +QL   SNE++EEKR  +D   E K+  Q    +E+K+ L+  ++  +I++
Sbjct: 990  NKAQMLELKEQLDLKSNEVNEEKRKLADSNNEEKNLKQNLELIELKSQLQ--HIESEISR 1047

Query: 263  SD 268
             D
Sbjct: 1048 LD 1049
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,305,377
Number of Sequences: 369166
Number of extensions: 1044243
Number of successful extensions: 3196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3195
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5636246860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)