Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_O20
(518 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST... 88 1e-17
sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-si... 87 2e-17
sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST... 84 2e-16
sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST... 80 4e-15
sp|O18598|GST1_BLAGE Glutathione S-transferase (GST class-s... 77 2e-14
sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST... 76 4e-14
sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D... 76 4e-14
sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (... 75 1e-13
sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class... 73 4e-13
sp|P91252|GST6_CAEEL Probable glutathione S-transferase 6 (... 72 6e-13
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
class-mu)
Length = 206
Score = 88.2 bits (217), Expect = 1e-17
Identities = 53/161 (32%), Positives = 91/161 (56%), Gaps = 9/161 (5%)
Frame = +2
Query: 5 PTGKLPVVEYEGKK-----LTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG 169
P G+LP+V+ K+ +++S+AIAR IARK+ MG+ D EYY+IE+ IGQ+ DV
Sbjct: 44 PGGRLPIVKITDKRGDVKTMSESLAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESE 103
Query: 170 MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTD 343
+K P E+KE + E + L+++ +KES VG+K+TLA++ + D
Sbjct: 104 YHKTLIKPPEEKEKISKEILNGKVPILLQAICETLKESTGNLTVGDKVTLADVVLIASID 163
Query: 344 YLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
++ L K L +P++ + R+ +L + + ++ R T
Sbjct: 164 HITDLDKEFLTGKYPEIHKHRKHLLATSPKLAKYLSERHAT 204
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
Length = 203
Score = 87.0 bits (214), Expect = 2e-17
Identities = 55/154 (35%), Positives = 87/154 (56%), Gaps = 5/154 (3%)
Frame = +2
Query: 17 LPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPE 196
+PV++ +G K++QSM IAR +AR++G G+ E Y ++ + D+ + KI FAPE
Sbjct: 51 MPVLDIDGTKMSQSMCIARHLAREFGLDGKTSLEKYRVDEITETLQDIFNDVVKIKFAPE 110
Query: 197 EKKEDLRTEFVTTSGRDNLKSLEGFIKES----GFFVGEKITLAELF-FVVLTDYLEKLP 361
KE ++ + + R LEG + + GFFVG +TLA+L +V L L+ P
Sbjct: 111 AAKEAVQQNYEKSCKR-LAPFLEGLLVSNGGGDGFFVGNSMTLADLHCYVALEVPLKHTP 169
Query: 362 KLYDDFPKLKELRERVLKANTDVEDWVKTRPVTD 463
+L D PK+ LR+RV + + ++K RPV D
Sbjct: 170 ELLKDCPKIVALRKRVAEC-PKIAAYLKKRPVRD 202
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
(Protective 28 kDa antigen) (GST class-mu)
Length = 211
Score = 84.3 bits (207), Expect = 2e-16
Identities = 53/161 (32%), Positives = 88/161 (54%), Gaps = 9/161 (5%)
Frame = +2
Query: 5 PTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG 169
P G+LP V+ K + +S+AIAR +A+K+ MGE D+EYY +E+ IGQ DV
Sbjct: 49 PGGRLPAVKVTDDHGHVKWMLESLAIARYMAKKHHMMGETDEEYYSVEKLIGQAEDVEHE 108
Query: 170 MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTD 343
+K P+E+KE + E + L + +K S VG+K+TLA+L + + D
Sbjct: 109 YHKTLMKPQEEKEKITKEILNGKVPVLLNMICESLKGSTGKLAVGDKVTLADLVLIAVID 168
Query: 344 YLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
++ L K L +P++ + RE +L ++ + ++ RP T
Sbjct: 169 HVTDLDKGFLTGKYPEIHKHRENLLASSPRLAKYLSNRPAT 209
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 79.7 bits (195), Expect = 4e-15
Identities = 51/161 (31%), Positives = 88/161 (54%), Gaps = 9/161 (5%)
Frame = +2
Query: 5 PTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG 169
P G+LP V+ K + +S+AIAR +A+K+ MGE D+EYY +E+ IGQ+ D+
Sbjct: 49 PGGRLPAVKITDNHGHVKWMLESLAIARYMAKKHHMMGETDEEYYNVEKLIGQVEDLEHE 108
Query: 170 MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTD 343
+K PEE+K+ + E + L + +K S VG+K+TLA+L + + D
Sbjct: 109 YHKTLMKPEEEKQKITKEILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIAVID 168
Query: 344 YLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
++ L K L +P++ + RE +L ++ + ++ R T
Sbjct: 169 HVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKYLSDRAAT 209
>sp|O18598|GST1_BLAGE Glutathione S-transferase (GST class-sigma) (Major allergen Bla g
5)
Length = 204
Score = 77.0 bits (188), Expect = 2e-14
Identities = 52/163 (31%), Positives = 84/163 (51%), Gaps = 9/163 (5%)
Frame = +2
Query: 5 PTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIY 184
P GK PV+E +GK+ QS+AI+R + +++G G+DD E I+ + + D + +
Sbjct: 48 PFGKTPVLEIDGKQTHQSVAISRYLGKQFGLSGKDDWENLEIDMIVDTISDFRAAIANYH 107
Query: 185 FAPEE-----KKEDLRTEFVT--TSGRDNLKSLEGFIKESGFFVGEKITLAELFFVVLTD 343
+ +E K + L+ E + T D + G G+ K+T A+ +FV + D
Sbjct: 108 YDADENSKQKKWDPLKKETIPYYTKKFDEVVKANG-----GYLAAGKLTWADFYFVAILD 162
Query: 344 YLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVTDM 466
YL + K L + P LK LRE+VL ++ WV RP TD+
Sbjct: 163 YLNHMAKEDLVANQPNLKALREKVLGLPA-IKAWVAKRPPTDL 204
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 76.3 bits (186), Expect = 4e-14
Identities = 50/161 (31%), Positives = 86/161 (53%), Gaps = 9/161 (5%)
Frame = +2
Query: 5 PTGKLPVVEYEG-----KKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG 169
P G+LP V+ K + +S+AIAR +A+K+ MG ++EYY +E+ IGQ D+
Sbjct: 49 PGGRLPAVKITDNHGHVKWMVESLAIARYMAKKHHMMGGTEEEYYNVEKLIGQAEDLEHE 108
Query: 170 MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKES--GFFVGEKITLAELFFVVLTD 343
YK PEE+K+ + E + L + +K S VG+K+TLA+L + + D
Sbjct: 109 YYKTLMKPEEEKQKIIKEILNGKVPVLLDIICESLKASTGKLAVGDKVTLADLVLIAVID 168
Query: 344 YLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
++ L K L +P++ + RE +L ++ + ++ R T
Sbjct: 169 HVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKYLSDRAAT 209
>sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D synthase
(Glutathione-dependent PGD synthetase) (Prostaglandin-H2
D-isomerase) (Hematopoietic prostaglandin D synthase)
(H-PGDS)
Length = 199
Score = 76.3 bits (186), Expect = 4e-14
Identities = 49/157 (31%), Positives = 85/157 (54%), Gaps = 5/157 (3%)
Frame = +2
Query: 5 PTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIY 184
P GK+P++E +G + QS+AIAR +AR+ G G+ E L + + + D + +
Sbjct: 47 PFGKVPILEVDGVIIHQSLAIARYLARESGLAGQTPVEQALADAIVDTIDD-----FMML 101
Query: 185 FAPEEKKEDLR----TEFVTTSGRDNLKSLEGFIKESGFFVGEKITLAELFF-VVLTDYL 349
F EK +D++ + +T + LK L+ F+ + +FVG+ +T A+ ++ V T L
Sbjct: 102 FPWAEKNQDVKEKAFNDILTNKAPELLKDLDTFLGDKKWFVGKSVTWADFYWDVCSTTLL 161
Query: 350 EKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
L D +P+L LR+RV +A + W++ RP T
Sbjct: 162 SYKADLADKYPRLLALRDRV-EALPAIAAWIQKRPKT 197
>sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma)
Length = 206
Score = 74.7 bits (182), Expect = 1e-13
Identities = 54/161 (33%), Positives = 83/161 (51%), Gaps = 8/161 (4%)
Frame = +2
Query: 2 TPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEG---- 169
TP G+LP++E +GK L QS AIAR +AR++G G+ E + Q D L
Sbjct: 46 TPFGQLPLLEVDGKVLAQSHAIARYLARQFGINGKCAWEEAQVNSVADQFKDYLNEVRPY 105
Query: 170 -MYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKE--SGFFVGEKITLAELFFVVLT 340
M K+ FA E + L + + + F+K SG+ VG+ +T +L T
Sbjct: 106 FMVKMGFA-EGDLDALAKDVFLPGFKKHYGFFANFLKSAGSGYLVGDSLTFVDLLVAQHT 164
Query: 341 -DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 460
D L L D+FP+ K +E+V +N +++ W++TRPVT
Sbjct: 165 ADLLAANAALLDEFPQFKAHQEKV-HSNANIKKWLETRPVT 204
>sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class-sigma)
Length = 203
Score = 73.2 bits (178), Expect = 4e-13
Identities = 47/163 (28%), Positives = 87/163 (53%), Gaps = 12/163 (7%)
Frame = +2
Query: 2 TPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKI 181
TP G++PV+E +GKK QS+AI+R + RKYG G D +E + I++ + + D+ +
Sbjct: 45 TPFGQMPVLEIDGKKYAQSLAISRYLGRKYGLAGNDIEEDFEIDQIVDFVNDIRASAASV 104
Query: 182 YFAPEEKKEDLRTEFVTTSGRDNLKSLEGF---------IKESGFFVGEKITLAELFFVV 334
+ + ++++ E +N+K+ F K +GF ++T A+ FV
Sbjct: 105 EYEQDAANKEVKHE-------ENMKNKYPFQLNKLSEIITKNNGFLALGRLTWADFVFVG 157
Query: 335 LTDYLEK---LPKLYDDFPKLKELRERVLKANTDVEDWVKTRP 454
+ DYL+K +P L + +P K+ E VL +N ++ ++ + P
Sbjct: 158 MFDYLKKMLRMPDLEEQYPIFKKPIETVL-SNPKLKAYLDSAP 199
>sp|P91252|GST6_CAEEL Probable glutathione S-transferase 6 (GST class-sigma)
Length = 206
Score = 72.4 bits (176), Expect = 6e-13
Identities = 49/159 (30%), Positives = 83/159 (52%), Gaps = 8/159 (5%)
Frame = +2
Query: 2 TPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKI 181
TP G+LP++E +GK L QS AIAR +AR++G G++D E ++ Q D L +
Sbjct: 46 TPFGQLPILEVDGKPLGQSYAIARYLAREFGIAGQNDTEAAEVDAIADQFKDYLNDVSPY 105
Query: 182 Y-----FAPEEKKEDLRTEFVTTSGRDNLKSLEGFI--KESGFFVGEKITLAELFFVV-L 337
F P + K+ LRT+ + + N + E + SGFFVG +T +L +
Sbjct: 106 LTVLAGFKPGD-KDQLRTDVFVPAFKKNFEFFENILASNHSGFFVGNSLTWVDLLISQHV 164
Query: 338 TDYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRP 454
D L+K + ++F K+ R++V + ++ ++ RP
Sbjct: 165 QDILDKDLAVVEEFKKVLAHRKKVQSIDR-IQKYIANRP 202
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,593,798
Number of Sequences: 369166
Number of extensions: 1213682
Number of successful extensions: 4079
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3943
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4047
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3403581975
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)