Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_O13
(848 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 107 3e-23
sp|P41731|CD63_MOUSE CD63 antigen 102 1e-21
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 102 2e-21
sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell s... 100 7e-21
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 100 9e-21
sp|Q28709|CD63_RABIT CD63 antigen 96 2e-19
sp|Q9XSK2|CD63_BOVIN CD63 antigen 95 3e-19
sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane... 91 5e-18
sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23) 91 5e-18
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 90 7e-18
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 107 bits (268), Expect = 3e-23
Identities = 63/215 (29%), Positives = 106/215 (49%), Gaps = 12/215 (5%)
Frame = +2
Query: 98 CGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVLL 277
C + LIA+G V K + T+ S F+GCCGA +E+ L+
Sbjct: 24 CAVGLIAIGVAVQVVLKQAITHETTAGSLLPVVIIAVGAFLFLVAFVGCCGACKENYCLM 83
Query: 278 IIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQQ 457
I F + L ++++ ++A+ IA YV+R VK+ +KS + Y T DN + ++D Q+
Sbjct: 84 ITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFSKSFQKQMQNYLT--DNKTATILDKLQK 141
Query: 458 LFKCCGVNGTTDW-------GNNIPNSCCES-----NRACNATTAFPQNCLTAMIDKIQS 601
KCCG + TDW + +P+SCC + +T Q C+ + ++
Sbjct: 142 ENKCCGASNYTDWERIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAAWLRK 201
Query: 602 QIGILAGVALLVCLIEIIGMILAFFLSKSIAEEYE 706
+ ++AG AL + +E++G+I + L KSI YE
Sbjct: 202 NVLLVAGAALGIAFVEVLGIIFSCCLVKSIRSGYE 236
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 102 bits (254), Expect = 1e-21
Identities = 62/215 (28%), Positives = 102/215 (47%), Gaps = 12/215 (5%)
Frame = +2
Query: 98 CGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVLL 277
C + LIA+G V K + T+ S F+GCCGA +E+ L+
Sbjct: 24 CAVGLIAIGVAVQVVLKQAITHETTAGSLLPVVIIAVGAFLFLVAFVGCCGACKENYCLM 83
Query: 278 IIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQQ 457
I F + L ++++ ++A+ IA YV+R VK+ KS + Y DN + ++D Q+
Sbjct: 84 ITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLK--DNKTATILDKLQK 141
Query: 458 LFKCCGVNGTTDWGN-------NIPNSCCES-----NRACNATTAFPQNCLTAMIDKIQS 601
CCG + TDW N +P+SCC + +T Q C+ + ++
Sbjct: 142 ENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAIWLRK 201
Query: 602 QIGILAGVALLVCLIEIIGMILAFFLSKSIAEEYE 706
I ++A AL + +E++G+I + L KSI YE
Sbjct: 202 NILLVAAAALGIAFVEVLGIIFSCCLVKSIRSGYE 236
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 102 bits (253), Expect = 2e-21
Identities = 54/153 (35%), Positives = 89/153 (58%)
Frame = +2
Query: 233 FLGCCGAIQESKVLLIIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRT 412
FLGC G+I+E+K LL+ F V L+++L+A++ L I ++VY + T+VA+ +ND++ Y +
Sbjct: 68 FLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEKKINTLVAEGLNDSIQHYHS 127
Query: 413 SYDNTSRILVDNTQQLFKCCGVNGTTDWGNNIPNSCCESNRACNATTAFPQNCLTAMIDK 592
DN++R+ D Q +CCGVNG++DW + P+SC + A Q C
Sbjct: 128 --DNSTRMAWDFIQSQLQCCGVNGSSDWISGPPSSC--------PSGADVQGCYKKGQAW 177
Query: 593 IQSQIGILAGVALLVCLIEIIGMILAFFLSKSI 691
S + V + VC+I+++GM A L+ I
Sbjct: 178 FHSNFLYIGIVTICVCVIQVLGMSFALTLNCQI 210
>sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Tetraspanin-25) (Tspan-25)
Length = 219
Score = 100 bits (248), Expect = 7e-21
Identities = 49/153 (32%), Positives = 85/153 (55%)
Frame = +2
Query: 233 FLGCCGAIQESKVLLIIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRT 412
FLGC G+I+E+K LL+ F + L+++L+A++ L I ++VY + VAK + D+++ Y +
Sbjct: 68 FLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQKLNEYVAKGLTDSIHRYHS 127
Query: 413 SYDNTSRILVDNTQQLFKCCGVNGTTDWGNNIPNSCCESNRACNATTAFPQNCLTAMIDK 592
DN+++ D+ Q +CCG+NGT+DW + P SC + + C
Sbjct: 128 --DNSTKAAWDSIQSFLQCCGINGTSDWTSGPPASCPSDRKV--------EGCYAKARLW 177
Query: 593 IQSQIGILAGVALLVCLIEIIGMILAFFLSKSI 691
S + + + VC+IE++GM A L+ I
Sbjct: 178 FHSNFLYIGIITICVCVIEVLGMSFALTLNCQI 210
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 99.8 bits (247), Expect = 9e-21
Identities = 56/199 (28%), Positives = 95/199 (47%)
Frame = +2
Query: 95 ICGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVL 274
+CG ++ G L F FLGC G+I+E+K L
Sbjct: 22 VCGCCILGFGIYFLVQNTYGVLFRNLPFLTLGNILVIVGSIIMVVAFLGCMGSIKENKCL 81
Query: 275 LIIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQ 454
L+ F V L+++L+A++ + I ++VY + T+VA+ +ND++ Y + DN++ D Q
Sbjct: 82 LMSFFVLLLIILLAEVTIAILLFVYEQKLNTLVAEGLNDSIQHYHS--DNSTMKAWDFIQ 139
Query: 455 QLFKCCGVNGTTDWGNNIPNSCCESNRACNATTAFPQNCLTAMIDKIQSQIGILAGVALL 634
+CCGVNG++DW + P+SC + A Q C S + + +
Sbjct: 140 TQLQCCGVNGSSDWTSGPPSSC--------PSGADVQGCYNKAKSWFHSNFLYIGIITIC 191
Query: 635 VCLIEIIGMILAFFLSKSI 691
VC+I+++GM A L+ I
Sbjct: 192 VCVIQVLGMSFALTLNCQI 210
>sp|Q28709|CD63_RABIT CD63 antigen
Length = 238
Score = 95.5 bits (236), Expect = 2e-19
Identities = 58/215 (26%), Positives = 99/215 (46%), Gaps = 12/215 (5%)
Frame = +2
Query: 98 CGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVLL 277
C + LIA+G V + T S F+GCCG +E+ L+
Sbjct: 24 CAVGLIAVGVGAQLVLSQTITHGATPGSLLPVVIIAVGAFLFLVAFVGCCGTCKENYCLM 83
Query: 278 IIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQQ 457
I F + L ++++ ++A IA YV+R V + K + Y T DN + +++D Q+
Sbjct: 84 ITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNKDFRQQMQNYST--DNQTALILDRMQK 141
Query: 458 LFKCCGVNGTTDWG-------NNIPNSCCES-NRAC----NATTAFPQNCLTAMIDKIQS 601
F CCG TDW + +P+SCC + C N + + C+ + ++
Sbjct: 142 DFTCCGAANYTDWATIPGMTRDRVPDSCCVNVTSGCGVKFNVKDIYVEGCVEKIGLWLRK 201
Query: 602 QIGILAGVALLVCLIEIIGMILAFFLSKSIAEEYE 706
+ ++A AL + +E++G++ A L KSI YE
Sbjct: 202 NVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYE 236
>sp|Q9XSK2|CD63_BOVIN CD63 antigen
Length = 237
Score = 94.7 bits (234), Expect = 3e-19
Identities = 60/214 (28%), Positives = 99/214 (46%), Gaps = 11/214 (5%)
Frame = +2
Query: 98 CGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVLL 277
C + LIA+G T V + T F+GCCGA +E+ L+
Sbjct: 24 CAVGLIAVGVGTHLVLNQTITHGATPSFLLPVVIIAVGAFLFLVAFVGCCGACKENYCLM 83
Query: 278 IIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQQ 457
I F + L ++++ ++A IA YV+R V++ K + Y DN + ++D Q+
Sbjct: 84 ITFAIFLSLIMLVEVAAAIAGYVFRDKVRSEFNKDFRQQMKNY--PKDNQTASILDKMQK 141
Query: 458 LFKCCGVNGTTDW------GNNIPNSCCES---NRACN--ATTAFPQNCLTAMIDKIQSQ 604
F+CCG TDW N +P+SCC + N N + C+ + ++
Sbjct: 142 DFECCGAANYTDWEKILAVTNKVPDSCCVNITHNCGINFVVKDIHTEGCVEKIAAWLRKN 201
Query: 605 IGILAGVALLVCLIEIIGMILAFFLSKSIAEEYE 706
+ ++ AL + +EI+G++LA L KSI YE
Sbjct: 202 VLVVVAAALGIAFVEILGIVLACCLVKSIRSGYE 235
>sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane 4 superfamily member 3)
(Tumor-associated antigen CO-029)
Length = 237
Score = 90.5 bits (223), Expect = 5e-18
Identities = 57/211 (27%), Positives = 109/211 (51%), Gaps = 16/211 (7%)
Frame = +2
Query: 95 ICGLALIALGAVTLTVYKNSTPL-----LGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQ 259
+CG+ ++AL A+ + V +S + +G+S FLGCCGAI+
Sbjct: 21 LCGILILAL-AIWVRVSNDSQAIFGSEDVGSSSYVAVDILIAVGAIIMILGFLGCCGAIK 79
Query: 260 ESKVLLIIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRI- 436
ES+ +L++F + L+++L+ Q+A I V++S IV +++ + + ++ +
Sbjct: 80 ESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSDRIVNETLYENTKLLSATGESEKQFQ 139
Query: 437 -LVDNTQQLFKCCG-VNGTTDWGNNIPN-----SCCESNRAC---NATTAFPQNCLTAMI 586
+ Q+ FKCCG VNG DWGNN + +C + R C N + + C++ +
Sbjct: 140 EAIIVFQEEFKCCGLVNGAADWGNNFQHYPELCACLDKQRPCQSYNGKQVYKETCISFIK 199
Query: 587 DKIQSQIGILAGVALLVCLIEIIGMILAFFL 679
D + + I+ G++ + +IEI+G++ + L
Sbjct: 200 DFLAKNLIIVIGISFGLAVIEILGLVFSMVL 230
>sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23)
Length = 218
Score = 90.5 bits (223), Expect = 5e-18
Identities = 59/206 (28%), Positives = 93/206 (45%)
Frame = +2
Query: 95 ICGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVL 274
+C L LI GA + L + FLGCCGAI+E+ +
Sbjct: 24 LCSLVLIGAGAYVEVKFSQYGDNLHKVWQAAPIAIIVVGVIILIVSFLGCCGAIKENVCM 83
Query: 275 LIIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQ 454
L ++ LI+LLIA++A I VY+ + + + M A++ +D Q
Sbjct: 84 LYMYAFFLIILLIAELAAAIVAVVYKDRIDSEIDALMTGALDKPTPEITE----FMDLIQ 139
Query: 455 QLFKCCGVNGTTDWGNNIPNSCCESNRACNATTAFPQNCLTAMIDKIQSQIGILAGVALL 634
F CCG G D+G NIP SC TT + + C+ ++ + I+A VA
Sbjct: 140 SSFHCCGAKGPQDYGPNIPASC------RGETTVYHEGCVPVFGAFLKRNLVIVACVAFG 193
Query: 635 VCLIEIIGMILAFFLSKSIAEEYEKV 712
VC +++ +++A L + I +EYE V
Sbjct: 194 VCFFQLLSIVIACCLGRQI-KEYENV 218
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 90.1 bits (222), Expect = 7e-18
Identities = 58/215 (26%), Positives = 96/215 (44%), Gaps = 12/215 (5%)
Frame = +2
Query: 98 CGLALIALGAVTLTVYKNSTPLLGTSFSNXXXXXXXXXXXXXXXXFLGCCGAIQESKVLL 277
C + LIA+G V + T S F+GCCGA +E+ L+
Sbjct: 24 CAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLM 83
Query: 278 IIFGVCLIVLLIAQIALCIAVYVYRSNVKTIVAKSMNDAVNTYRTSYDNTSRILVDNTQQ 457
I F + L ++++ ++A IA YV+R V + + + Y +N + ++D Q
Sbjct: 84 ITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENY--PKNNHTASILDRMQA 141
Query: 458 LFKCCGVNGTTDW-------GNNIPNSCC-ESNRAC----NATTAFPQNCLTAMIDKIQS 601
FKCCG TDW N +P+SCC C N + C+ + ++
Sbjct: 142 DFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRK 201
Query: 602 QIGILAGVALLVCLIEIIGMILAFFLSKSIAEEYE 706
+ ++A AL + +E++G++ A L KSI YE
Sbjct: 202 NVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYE 236
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,142,976
Number of Sequences: 369166
Number of extensions: 1707236
Number of successful extensions: 5093
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5005
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)