Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_O09
(393 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P39001|UME6_YEAST Transcriptional regulator UME6 (Negati... 31 0.78
sp|P53963|YND4_YEAST Hypothetical 69.4 kDa protein in NCE3-... 30 1.3
sp|P53976|YNB8_YEAST Hypothetical 69.6 kDa protein in HDA1-... 30 1.7
sp|P13483|DATI_YEAST Oligo(A)/oligo(T)-binding protein (Datin) 30 2.3
sp|Q7UZZ9|IF2_PROMP Translation initiation factor IF-2 28 5.1
sp|Q24174|ABRU_DROME Abrupt protein (Clueless protein) 28 5.1
sp|P23637|ERF2_PICPI Eukaryotic peptide chain release facto... 28 6.6
sp|P50077|CCH1_YEAST Probable calcium-channel protein 28 6.6
sp|P27625|RPC1_PLAFA DNA-directed RNA polymerase III larges... 28 6.6
sp|Q7VRU3|SYE_BLOFL Glutamyl-tRNA synthetase (Glutamate--tR... 28 8.6
>sp|P39001|UME6_YEAST Transcriptional regulator UME6 (Negative transcriptional regulator
of IME2)
Length = 836
Score = 31.2 bits (69), Expect = 0.78
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1
Query: 4 YNRSHSMNENSAGIRNSDWNGYSSYTSPNDSHLSSFQEGNFNMMPMRVPNVQCTPANVSG 183
Y+ + S + N++ ++ N + TS + + ++FQ FN +P + P + + +
Sbjct: 378 YSLNESSSNNASSNTDTPTNSRHANTSSSITSRNNFQHFRFNQIPSQPPTSASSFTSTNN 437
Query: 184 NDPQ 195
N+PQ
Sbjct: 438 NNPQ 441
>sp|P53963|YND4_YEAST Hypothetical 69.4 kDa protein in NCE3-HHT2 intergenic region
Length = 612
Score = 30.4 bits (67), Expect = 1.3
Identities = 22/74 (29%), Positives = 33/74 (44%)
Frame = +1
Query: 67 YSSYTSPNDSHLSSFQEGNFNMMPMRVPNVQCTPANVSGNDPQLFSRQQTSHSVNH*QKI 246
YSS+ P+ H+SSF +GNF + N + T + F R + S + + I
Sbjct: 212 YSSWNPPHSPHISSFPDGNFASL-----NAEVTCFD--------FRRTKDSRTKETNESI 258
Query: 247 IVFNYYYYIKSSTH 288
I Y I S+ H
Sbjct: 259 IPTEIYCPINSTDH 272
>sp|P53976|YNB8_YEAST Hypothetical 69.6 kDa protein in HDA1-PUB1 intergenic region
Length = 612
Score = 30.0 bits (66), Expect = 1.7
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 67 YSSYTSPNDSHLSSFQEGNF 126
YSS+ P+ H+SSF +GNF
Sbjct: 212 YSSWNPPHSPHISSFPDGNF 231
>sp|P13483|DATI_YEAST Oligo(A)/oligo(T)-binding protein (Datin)
Length = 248
Score = 29.6 bits (65), Expect = 2.3
Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 5/69 (7%)
Frame = +1
Query: 4 YNRSHSMNENSAGIRNSDWNGYSSYT--SPNDSHLSSFQEGNFNMM--PMRV-PNVQCTP 168
YN HS+N NS NS+ N ++T N SSF G +N + P V P + +
Sbjct: 158 YNADHSINHNSNHNLNSNVNVNMNFTINGSNQDPSSSFLMGPYNYLQRPFIVKPYLDLST 217
Query: 169 ANVSGNDPQ 195
+ + N P+
Sbjct: 218 STAASNQPR 226
>sp|Q7UZZ9|IF2_PROMP Translation initiation factor IF-2
Length = 1169
Score = 28.5 bits (62), Expect = 5.1
Identities = 20/71 (28%), Positives = 32/71 (45%), Gaps = 4/71 (5%)
Frame = +1
Query: 25 NENSAGIRNSDWNGYSSYTSPNDSHLSSFQEGNFNMMPMRVPNVQCTP----ANVSGNDP 192
N N+ I NS S +++ LS NF + PNV+ TP A + DP
Sbjct: 203 NINANKINNSVNQKAQSLNRADNNKLSRADNNNFPKKNLNSPNVKSTPELVGAPIRREDP 262
Query: 193 QLFSRQQTSHS 225
++ + + S+S
Sbjct: 263 KINTNRPNSNS 273
>sp|Q24174|ABRU_DROME Abrupt protein (Clueless protein)
Length = 904
Score = 28.5 bits (62), Expect = 5.1
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = +1
Query: 13 SHSMNENSAGIRNSDWNGYSSYTSPNDSHLSSFQEGNFNMMPMRVPNVQCTPANVSGN 186
S++ N N+ +S+ N SSY SPN + + N N + + N+SG+
Sbjct: 222 SNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGS 279
>sp|P23637|ERF2_PICPI Eukaryotic peptide chain release factor GTP-binding subunit (ERF2)
(Translation release factor 3) (ERF3) (ERF-3)
(Omnipotent suppressor protein 2)
Length = 741
Score = 28.1 bits (61), Expect = 6.6
Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +1
Query: 4 YNRSHSMNENSAGIRNSDWNGYSSYTSPN-DSHLSSFQEGNFN 129
YN + N G NS+ GYS+Y S N +S+ + N N
Sbjct: 79 YNNRGGYSNNRGGYNNSNRGGYSNYNSYNTNSNQGGYSNYNNN 121
>sp|P50077|CCH1_YEAST Probable calcium-channel protein
Length = 2039
Score = 28.1 bits (61), Expect = 6.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +1
Query: 172 NVSGNDPQLFSRQQTSHSVNH*QKIIVFNYYYYIKSSTHRVRKF 303
+++G L + +++ +K +FNY YY S HR R+F
Sbjct: 1125 DINGRTYILELNEYEDEKLDYLKKYPLFNYSYYFFSPQHRFRRF 1168
>sp|P27625|RPC1_PLAFA DNA-directed RNA polymerase III largest subunit
Length = 2339
Score = 28.1 bits (61), Expect = 6.6
Identities = 19/61 (31%), Positives = 27/61 (44%)
Frame = +1
Query: 7 NRSHSMNENSAGIRNSDWNGYSSYTSPNDSHLSSFQEGNFNMMPMRVPNVQCTPANVSGN 186
N H+ N N I N+D N S NDS+++S + NM + N N+ N
Sbjct: 1109 NSIHNNNSNMNNIHNNDSN--RSIIHNNDSNMNSIHNNDSNM--NSIHNNNSNMNNIHNN 1164
Query: 187 D 189
D
Sbjct: 1165 D 1165
>sp|Q7VRU3|SYE_BLOFL Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
Length = 483
Score = 27.7 bits (60), Expect = 8.6
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 7/72 (9%)
Frame = +1
Query: 199 FSRQQTSHSVNH*QKIIVFNYYYYIKSSTHRVRKFLNDY-------DINQLSILNDFY*L 357
FS S SV K++ FN+YY + + K+L++Y DI+ + +++
Sbjct: 301 FSEIHKSASVFDINKLMWFNHYYINHLPVNHIIKYLSEYIQIQNISDIDSVKLIDLVNLF 360
Query: 358 SKNRVNKKPVLF 393
SK K ++F
Sbjct: 361 SKRSHTLKEMIF 372
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,697,688
Number of Sequences: 369166
Number of extensions: 741369
Number of successful extensions: 1624
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1590
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1622
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1721094280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)