Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_N01
(805 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-l... 140 5e-33
sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin... 127 3e-29
sp|P47796|CTRA_GADMO Chymotrypsin A precursor 126 8e-29
sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4) 124 2e-28
sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8) 123 5e-28
sp|P40313|CTRL_HUMAN Chymotrypsin-like protease CTRL-1 prec... 123 7e-28
sp|P00765|TRYP_ASTFL Trypsin I 120 3e-27
sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) ... 120 3e-27
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 120 4e-27
sp|Q9N2D1|TRYT_PIG Tryptase precursor 117 5e-26
>sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-like protein 4) (KLK-L4)
Length = 277
Score = 140 bits (352), Expect = 5e-33
Identities = 88/249 (35%), Positives = 125/249 (50%), Gaps = 1/249 (0%)
Frame = +1
Query: 4 TSGWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFK 183
TSG+ + G H P ++ VQG CGG L++ VLTAAHC+ +
Sbjct: 32 TSGF-LPGGYTCFPHSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE---------- 80
Query: 184 YYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLE-GNDVALIKFDGYISKNQ 360
+KVY+G++ L R+ E++ +V +I HP YR +L +D+ L++ +
Sbjct: 81 --GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLT- 137
Query: 361 YDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNV 540
YI L N PG C V GWG+ P + + LQ A I + S+ EC +++
Sbjct: 138 -GYIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG- 195
Query: 541 AYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTR 720
+ +CAG+ SC GDSGGPL C + GIVS+G PCGQP+ P VYTR
Sbjct: 196 KITDNMLCAGTKEGGKDSCEGDSGGPLVC-----NRTLYGIVSWGDFPCGQPDRPGVYTR 250
Query: 721 VSEVAGWIR 747
VS WIR
Sbjct: 251 VSRYVLWIR 259
>sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin B chain A; Chymotrypsin B
chain B]
Length = 245
Score = 127 bits (320), Expect = 3e-29
Identities = 89/251 (35%), Positives = 127/251 (50%), Gaps = 3/251 (1%)
Frame = +1
Query: 1 VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 177
VT +I+NG +A+ H +P VS++ G HFCGG+L+N N V+TAAHC NV T
Sbjct: 10 VTGYARIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHC-------NVRT 62
Query: 178 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKF--DGYIS 351
+ +V +GE+D ++ I + + HP + ND++LIK +
Sbjct: 63 YH----RVIVGEHDKASDENIQ-ILKPSMVFTHPKWDSRTI---NNDISLIKLASPAVLG 114
Query: 352 KNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIF 531
N DV A PG +C GWG T LQ+AA+ ++SN EC + +
Sbjct: 115 TNVSPVCLGESSDVFA---PGMKCVTSGWGLTRYNAPGTPNKLQQAALPLMSNEECSQTW 171
Query: 532 SNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSV 711
N ICAG+ +SC GDSGGPL C + VGIVS+G+ C P+V
Sbjct: 172 GNNMISDVMICAGAA--GATSCMGDSGGPLVCQKDNVWTL-VGIVSWGSSRC-SVTTPAV 227
Query: 712 YTRVSEVAGWI 744
Y RV+E+ GW+
Sbjct: 228 YARVTELRGWV 238
>sp|P47796|CTRA_GADMO Chymotrypsin A precursor
Length = 263
Score = 126 bits (316), Expect = 8e-29
Identities = 82/250 (32%), Positives = 131/250 (52%), Gaps = 2/250 (0%)
Frame = +1
Query: 1 VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 177
+T +I+NG +A+ H + VS++ Q G HFCGG+L+N N V+TAAHC +++N++
Sbjct: 28 ITGYSRIVNGEEAVPHSWSWQVSLQDQTGFHFCGGSLINENWVVTAAHC--NVKNYH--- 82
Query: 178 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKN 357
+V +GE+D ++ V + HP Y + ND+ L+K + N
Sbjct: 83 ------RVVLGEHDRSSNSEGVQVMTVGQVFKHPRYN---GFTINNDILLVKLATPATLN 133
Query: 358 -QYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 534
+ + E D + + G +C GWG DT LQ+AA+ +++N +C + +
Sbjct: 134 MRVSPVCLAETD--DVFEGGMKCVTSGWGLTRYNAADTPALLQQAALPLLTNEQCKKFWG 191
Query: 535 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 714
N S++ +G SSC GDSGGPL C + VGIVS+G+ C P +P VY
Sbjct: 192 NKI---SDLMICAGAAGASSCMGDSGGPLVCQKAGSWTL-VGIVSWGSGTC-TPTMPGVY 246
Query: 715 TRVSEVAGWI 744
RV+E+ W+
Sbjct: 247 ARVTELRAWV 256
>sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4)
Length = 324
Score = 124 bits (312), Expect = 2e-28
Identities = 90/259 (34%), Positives = 131/259 (50%), Gaps = 14/259 (5%)
Frame = +1
Query: 16 KIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVW---DMRNWNVNTFKY 186
+I+ G A + +P S++V GNH CG TLLN VLTAAHC D +W V
Sbjct: 54 RIVGGDDAELGRWPWQGSLRVWGNHLCGATLLNRRWVLTAAHCFQKDNDPFDWTV----- 108
Query: 187 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 366
Q L+ + +Q+ + P Y +Y ND+AL+K ++ N +
Sbjct: 109 -QFGELTSRPSLWNLQAYSNRYQIEDIFLSPKYSEQYP----NDIALLKLSSPVTYN--N 161
Query: 367 YIDYNELDVNAISKPGDR--CEVVGWGSMLPPGQDTS----RFLQKAAINIISNSECGRI 528
+I L +N+ K +R C V GWG++ G+D S LQ+ + II+NS C +
Sbjct: 162 FIQPICL-LNSTYKFENRTDCWVTGWGAI---GEDESLPSPNTLQEVQVAIINNSMCNHM 217
Query: 529 FSNVAYVRSNI-----CAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQ 693
+ + R+NI CAG+ +C GDSGGPL C D QVG+VS+G + CG+
Sbjct: 218 YKKPDF-RTNIWGDMVCAGTPEGGKDACFGDSGGPLAC-DQDTVWYQVGVVSWG-IGCGR 274
Query: 694 PNVPSVYTRVSEVAGWIRA 750
PN P VYT +S WI++
Sbjct: 275 PNRPGVYTNISHHYNWIQS 293
>sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8)
Length = 260
Score = 123 bits (309), Expect = 5e-28
Identities = 79/246 (32%), Positives = 116/246 (47%)
Frame = +1
Query: 10 GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 189
G KI+ G + I H P ++ CGG L+ VLTAAHC K
Sbjct: 30 GSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQ 77
Query: 190 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 369
+ V +G++ L E+ QV ++I HP Y + +D+ LI+ S N D
Sbjct: 78 KYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQN--SANLGDK 135
Query: 370 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 549
+ +L N K G +C + GWG++ P ++ L A + I S ++C R +
Sbjct: 136 VKPVQL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193
Query: 550 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 729
+CAGS +C+GDSGGPL C DG GI S+G+ PCG+P P VYT++
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVC-DGMLQ----GITSWGSDPCGKPEKPGVYTKICR 247
Query: 730 VAGWIR 747
WI+
Sbjct: 248 YTTWIK 253
>sp|P40313|CTRL_HUMAN Chymotrypsin-like protease CTRL-1 precursor
Length = 264
Score = 123 bits (308), Expect = 7e-28
Identities = 86/246 (34%), Positives = 127/246 (51%), Gaps = 3/246 (1%)
Frame = +1
Query: 16 KIINGSQAIMHEYPQMVSIK-VQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYYQ 192
+I+NG A++ +P VS++ G HFCGG+L++ + V+TAAHC NV+ +++
Sbjct: 33 RIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHC-------NVSPGRHF- 84
Query: 193 MKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD-- 366
V +GEYD ++ V++ I HPS+ + NDV L+K S QY
Sbjct: 85 --VVLGEYDRSSNAEPLQVLSVSRAITHPSWN---STTMNNDVTLLKL---ASPAQYTTR 136
Query: 367 YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAY 546
N G C GWG + G T LQ+ A+ +++ ++C R + +
Sbjct: 137 ISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQC-RQYWGSSI 195
Query: 547 VRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVS 726
S ICAG SSC+GDSGGPL C G + +GIVS+GT C P+VYTRVS
Sbjct: 196 TDSMICAGGA--GASSCQGDSGGPLVCQKGNT-WVLIGIVSWGTKNC-NVRAPAVYTRVS 251
Query: 727 EVAGWI 744
+ + WI
Sbjct: 252 KFSTWI 257
>sp|P00765|TRYP_ASTFL Trypsin I
Length = 237
Score = 120 bits (302), Expect = 3e-27
Identities = 82/254 (32%), Positives = 127/254 (50%), Gaps = 8/254 (3%)
Frame = +1
Query: 19 IINGSQAIMHEYPQMVSIKVQ----GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKY 186
I+ G+ A++ E+P +S + HFCG ++ N N +TA HCV+ N +
Sbjct: 1 IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSG--- 57
Query: 187 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 366
+++ GE D+ G E+ V+K I+H ++ ++ L ND++L+K G ++ N
Sbjct: 58 --LQIVAGELDMSVNEGSEQTITVSKIILHENFDYD---LLDNDISLLKLSGSLTFN--- 109
Query: 367 YIDYNELDVNAISKPGDRCE----VVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 534
N + A+ G V GWG+ G +T LQK + ++S++EC +
Sbjct: 110 ----NNVAPIALPAQGHTATGNVIVTGWGTT-SEGGNTPDVLQKVTVPLVSDAECRDDYG 164
Query: 535 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 714
S ICAG SC+GDSGGPL +D ++ GIVS+G C +P P VY
Sbjct: 165 ADEIFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYL-AGIVSWG-YGCARPGYPGVY 222
Query: 715 TRVSEVAGWIRANA 756
T VS WI+ANA
Sbjct: 223 TEVSYHVDWIKANA 236
>sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) (Brain serine protease 1)
Length = 260
Score = 120 bits (302), Expect = 3e-27
Identities = 77/246 (31%), Positives = 115/246 (46%)
Frame = +1
Query: 10 GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 189
G KI+ G + H P ++ CGG L+ VLTAAHC D KY
Sbjct: 30 GSKILEGQECKPHSQPWQTALFQGERLVCGGVLVGDRWVLTAAHCKKD---------KY- 79
Query: 190 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 369
V +G++ L + E+ QV ++I HP + +D+ LI+ S N D
Sbjct: 80 --SVRLGDHSLQKRDEPEQEIQVARSIQHPCFNSSNPEDHSHDIMLIRLQN--SANLGDK 135
Query: 370 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 549
+ EL N K G +C + GWG++ P ++ L A + I S ++C R +
Sbjct: 136 VKPIEL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193
Query: 550 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 729
+CAGS +C+GDSGGPL C + GI ++G+ PCG+P P VYT++
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVCNG-----VLQGITTWGSDPCGKPEKPGVYTKICR 247
Query: 730 VAGWIR 747
WI+
Sbjct: 248 YTNWIK 253
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 120 bits (301), Expect = 4e-27
Identities = 84/249 (33%), Positives = 125/249 (50%), Gaps = 6/249 (2%)
Frame = +1
Query: 16 KIINGSQAIMHEYPQMVSIKVQ---GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKY 186
+I+ G+ A + E+P VS++V+ H CGG+++ VLTAAHC + +V
Sbjct: 390 RIVGGTNASLGEWPWQVSLQVKLVSQTHLCGGSIIGRQWVLTAAHCFDGIPYPDV----- 444
Query: 187 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGN-DVALIKFDGYISKNQY 363
++Y G L + ++ + IIH EY EGN D+ALIK ++ ++
Sbjct: 445 --WRIYGGILSLSEITKETPSSRIKELIIH----QEYKVSEGNYDIALIKLQTPLNYTEF 498
Query: 364 D--YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSN 537
++ D N I C V GWG G+ T LQKA I ++ N EC + + +
Sbjct: 499 QKPICLPSKADTNTIYT---NCWVTGWGYTKEQGE-TQNILQKATIPLVPNEECQKKYRD 554
Query: 538 VAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYT 717
+ ICAG T +C+GDSGGPL C + + VGI S+G CG+ + P VYT
Sbjct: 555 YVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQL-VGITSWGE-GCGRKDQPGVYT 612
Query: 718 RVSEVAGWI 744
+VSE WI
Sbjct: 613 KVSEYMDWI 621
>sp|Q9N2D1|TRYT_PIG Tryptase precursor
Length = 275
Score = 117 bits (292), Expect = 5e-26
Identities = 87/260 (33%), Positives = 133/260 (51%), Gaps = 18/260 (6%)
Frame = +1
Query: 19 IINGSQAIMHEYPQMVSIKVQGN---HFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 189
I+ G +A H++P VS++ HFCGG+L++ VLTAAHC +
Sbjct: 31 IVGGKEAPGHKWPWQVSLRCLDQYWKHFCGGSLIHPQWVLTAAHC------FGPEKADPL 84
Query: 190 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 369
++V +GE LY +++ V++ I+HP+Y Y + G D+AL++ + + N +
Sbjct: 85 YIRVQLGEQHLYYQ---DRLLLVSRIIVHPNY---YDEVNGADIALLELEDPV--NLSSH 136
Query: 370 IDYNELDVNAISKP-GDRCEVVGWGSM-----LPPGQDTSRFLQKAAINIISNSECGRIF 531
+ L + + P G RC V GWG + LPP L++ + I+ NSEC +
Sbjct: 137 VQPVTLPPASETFPKGTRCWVTGWGDVHSGWPLPPPYP----LKQVRVPIVENSECDMQY 192
Query: 532 -------SNVAYVRSN-ICAGSGRLSTSSCRGDSGGPLFC-TDGRKHHIQVGIVSYGTVP 684
N+ VR + +CAGS SC+GDSGGPL C +G +Q G+VS+G
Sbjct: 193 HLGLSTGDNIPIVRDDMLCAGSE--GHDSCQGDSGGPLVCRVNGT--WLQAGVVSWGE-G 247
Query: 685 CGQPNVPSVYTRVSEVAGWI 744
C PN P +YTRV+ WI
Sbjct: 248 CALPNRPGIYTRVTHYLDWI 267
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,655,329
Number of Sequences: 369166
Number of extensions: 2174191
Number of successful extensions: 7002
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5904
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)