Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_M18-1
(604 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5VVX9|UBE2U_HUMAN Ubiquitin-conjugating enzyme E2 U (Ub... 37 0.050
sp|Q8RY73|Y4762_ARATH Putative protein At4g17620, chloropla... 35 0.19
sp|P23643|VPS3_YEAST Vacuolar protein sorting-associated pr... 33 0.56
sp|Q9URZ5|VPS1_SCHPO Vacuolar sorting protein 1 30 3.6
sp|Q8ET01|KHSE_OCEIH Homoserine kinase (HSK) (HK) 30 3.6
sp|Q63603|DOCK9_RAT Dedicator of cytokinesis protein 9 (Cdc... 30 3.6
sp|Q58634|PSMB_METJA Proteasome beta subunit precursor (Mul... 30 4.7
sp|Q95LM1|UBE2U_MACFA Ubiquitin-conjugating enzyme E2 U (Ub... 30 4.7
sp||P11722_2 [Segment 2 of 2] Fibronectin (FN) 30 4.7
>sp|Q5VVX9|UBE2U_HUMAN Ubiquitin-conjugating enzyme E2 U (Ubiquitin-protein ligase U)
(Ubiquitin carrier protein U)
Length = 321
Score = 36.6 bits (83), Expect = 0.050
Identities = 31/108 (28%), Positives = 49/108 (45%), Gaps = 1/108 (0%)
Frame = -1
Query: 415 NLKLSVIQHILSGKAIRYDVENEKFLLDTCPTL-PITDSASPFLLLVGGGSEEASFTDNG 239
NLK SVI+ L+ K + ++V + + CPTL P TD + + + S TD
Sbjct: 224 NLKYSVIKCWLARKRMPHEVTHSMEEIKLCPTLIPTTDE-----IFLESPTAINSITD-- 276
Query: 238 SGKSLAYKKHKLSTYSDTSVKPWELSQDDLKNENDSFSWTRNTDTNTN 95
Y+ + SDTS+ + + + D SWT +TNT+
Sbjct: 277 -----IYETEEEGWKSDTSLYENDTDEpreEEVEDLISWTNTLNTNTS 319
>sp|Q8RY73|Y4762_ARATH Putative protein At4g17620, chloroplast precursor
Length = 544
Score = 34.7 bits (78), Expect = 0.19
Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 8/71 (11%)
Frame = -1
Query: 277 GGGSEEASFTDNGSGKSLAYKKH---KLSTY-----SDTSVKPWELSQDDLKNENDSFSW 122
GGG++ S +D+GSG S ++H K+ +Y ++ V P+E +++ ++E D F
Sbjct: 60 GGGADSGSASDSGSGSSGGKEEHGDDKVESYRSNDNGESGVYPYEEEEEEEEDEKDLFGS 119
Query: 121 TRNTDTNTNPI 89
T T I
Sbjct: 120 DNEEYTKTPAI 130
>sp|P23643|VPS3_YEAST Vacuolar protein sorting-associated protein VPS3
Length = 1011
Score = 33.1 bits (74), Expect = 0.56
Identities = 19/52 (36%), Positives = 28/52 (53%)
Frame = -1
Query: 250 TDNGSGKSLAYKKHKLSTYSDTSVKPWELSQDDLKNENDSFSWTRNTDTNTN 95
T+N GK + + KL S++S P E D K E+DS T + +TNT+
Sbjct: 6 TNNDKGKEVKENEGKLDIDSESS--PHERENDKKKTEDDSLRATESEETNTH 55
>sp|Q9URZ5|VPS1_SCHPO Vacuolar sorting protein 1
Length = 678
Score = 30.4 bits (67), Expect = 3.6
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = -1
Query: 313 ITDSASPFLLLVGGGSEEASFTDNGSGKSLAYKKHKLSTYSDTSVKPWELSQD 155
ITD + + +V G SEE S T+ G +A+ H++ + ++ P++ +D
Sbjct: 347 ITDFCNEYRTVVDGRSEELSATELSGGARIAFVFHEIFSNGIQAIDPFDEVKD 399
>sp|Q8ET01|KHSE_OCEIH Homoserine kinase (HSK) (HK)
Length = 294
Score = 30.4 bits (67), Expect = 3.6
Identities = 25/92 (27%), Positives = 42/92 (45%), Gaps = 8/92 (8%)
Frame = -1
Query: 427 TANGNLKLSVI---QHILSGKAIRYDVENEKFLLDTCPTLP-ITDSASPF----LLLVGG 272
+A NL ++ + + L+GK + D+ +E F + P I + A + +L G
Sbjct: 190 SAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYGAFGTILSGA 249
Query: 271 GSEEASFTDNGSGKSLAYKKHKLSTYSDTSVK 176
G S T G+G +L KLS +D V+
Sbjct: 250 GPTMLSITPKGNGPTLQNNMSKLSLLADYQVE 281
>sp|Q63603|DOCK9_RAT Dedicator of cytokinesis protein 9 (Cdc42 guanine nucleotide
exchange factor zizimin 1) (TRG protein)
Length = 720
Score = 30.4 bits (67), Expect = 3.6
Identities = 21/59 (35%), Positives = 30/59 (50%)
Frame = -1
Query: 313 ITDSASPFLLLVGGGSEEASFTDNGSGKSLAYKKHKLSTYSDTSVKPWELSQDDLKNEN 137
IT +A + LL GG + F D GK YK+ KL+ S+ S + +L D +EN
Sbjct: 356 ITRAAGSWDLLPGGLFGQGFFEDE-DGKEYIYKEPKLTPLSEISQRLLKLYSDKFGSEN 413
>sp|Q58634|PSMB_METJA Proteasome beta subunit precursor (Multicatalytic endopeptidase
complex beta subunit)
Length = 224
Score = 30.0 bits (66), Expect = 4.7
Identities = 21/55 (38%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = -1
Query: 280 VGGGSEEASFTDNGSGKSLAYKKHKLSTYSDTSVKPW-ELSQDDLKN--ENDSFS 125
+GG +EE +FT GSG +AY + D SV+ +L+ + LK+ E D+FS
Sbjct: 124 LGGMNEEKTFTATGSGSPIAYGVLEAGYDRDMSVEEGIKLALNALKSAMERDTFS 178
>sp|Q95LM1|UBE2U_MACFA Ubiquitin-conjugating enzyme E2 U (Ubiquitin-protein ligase U)
(Ubiquitin carrier protein U)
Length = 322
Score = 30.0 bits (66), Expect = 4.7
Identities = 32/112 (28%), Positives = 49/112 (43%), Gaps = 6/112 (5%)
Frame = -1
Query: 415 NLKLSVIQHILSGK-----AIRYDVENEKFLLDTCPT-LPITDSASPFLLLVGGGSEEAS 254
NLK SVI+ L+ K +R+ +E K CPT +P TD + + + S
Sbjct: 224 NLKYSVIKCWLARKNRMPDEVRHSMEGIKL----CPTQIPTTDE-----IFLESPTAINS 274
Query: 253 FTDNGSGKSLAYKKHKLSTYSDTSVKPWELSQDDLKNENDSFSWTRNTDTNT 98
T+ + +K S Y + + +PWE +DL SW +TNT
Sbjct: 275 VTNIYETEEEGWKSDT-SLYENNTDEPWEEEVEDL------ISWINTLNTNT 319
>sp||P11722_2 [Segment 2 of 2] Fibronectin (FN)
Length = 1206
Score = 30.0 bits (66), Expect = 4.7
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +3
Query: 171 HGFTEVSEYVESLCFLYASDFPLPLSVKLASSDPPPTNNK 290
HG SEY ++ +Y LPL+ +++ PPPTN K
Sbjct: 609 HGLRPGSEYTINIVAIYDDMESLPLTGTQSTAIPPPTNLK 648
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,896,590
Number of Sequences: 369166
Number of extensions: 1369378
Number of successful extensions: 3428
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3318
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3424
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4650991475
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)