Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_M10
(220 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28988|CYTA1_PIG Cystatin A1 (Stefin A1) 44 1e-04
sp|P35479|CPI1_PIG Leukocyte cysteine proteinase inhibitor ... 43 2e-04
sp|Q8WNR9|CYTA_FELCA Cystatin A (Allergen Fel d 3) 43 3e-04
sp|P35173|CYT1_MOUSE Stefin 1 42 4e-04
sp|P35175|CYT3_MOUSE Stefin 3 40 0.001
sp|P80416|CYTA_BOVIN Cystatin A (Stefin A) (Cystatin AS) 40 0.001
sp|Q28987|CYTA8_PIG Cystatin A8 (Stefin A8) 40 0.002
sp|Q28986|CYTA5_PIG Cystatin A5 (Stefin A5) 40 0.002
sp|P56567|CYT4_MOUSE Stefin homolog 38 0.006
sp|P35174|CYT2_MOUSE Stefin 2 38 0.006
>sp|Q28988|CYTA1_PIG Cystatin A1 (Stefin A1)
Length = 103
Score = 43.9 bits (102), Expect = 1e-04
Identities = 22/60 (36%), Positives = 36/60 (60%), Gaps = 2/60 (3%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKL-YNKDEVQLMQCKI-ASKDEIL 202
EKFE + ++QVV GIN K+ +G Y+H+K+F L Y ++L ++ +KD+ L
Sbjct: 41 EKFEAVEYKSQVVAGINYYIKVHVGGNSYVHIKVFKSLPYQNKPLELSGYQVDKTKDDEL 100
>sp|P35479|CPI1_PIG Leukocyte cysteine proteinase inhibitor 1 (PLCPI) (Stefin D1)
Length = 103
Score = 43.1 bits (100), Expect = 2e-04
Identities = 22/60 (36%), Positives = 38/60 (63%), Gaps = 2/60 (3%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKL-YNKDEVQLMQCKI-ASKDEIL 202
EKFE I R+QVV G N K+ +G Y+H+++F L + +D ++L+ ++ +KD+ L
Sbjct: 41 EKFEAIIYRSQVVAGTNYYIKVHVGGNNYVHIRVFQSLPHQEDPLKLIGYQVDKTKDDEL 100
>sp|Q8WNR9|CYTA_FELCA Cystatin A (Allergen Fel d 3)
Length = 98
Score = 42.7 bits (99), Expect = 3e-04
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKD 154
+KFE I +TQVV GIN K+ + +YIH+K+F L +D
Sbjct: 36 QKFEAIEYKTQVVAGINYYIKVQVDDNRYIHIKVFKGLPVQD 77
>sp|P35173|CYT1_MOUSE Stefin 1
Length = 103
Score = 42.0 bits (97), Expect = 4e-04
Identities = 18/46 (39%), Positives = 32/46 (69%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDEVQL 166
EKFE + ++QVV G N+ KI +G+ ++H+K+F L +D+++L
Sbjct: 42 EKFEAVEYKSQVVAGQNLFIKIDVGNGCFLHMKVFRGLSGEDDLKL 87
>sp|P35175|CYT3_MOUSE Stefin 3
Length = 97
Score = 40.4 bits (93), Expect = 0.001
Identities = 19/46 (41%), Positives = 28/46 (60%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDEVQL 166
EKFE + +TQVV G N+ K+ +G +IH+K+F KD +L
Sbjct: 36 EKFEAVEYKTQVVAGENIFIKMDVGHGCFIHIKVFNGPTGKDNYEL 81
>sp|P80416|CYTA_BOVIN Cystatin A (Stefin A) (Cystatin AS)
Length = 98
Score = 40.4 bits (93), Expect = 0.001
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKL 142
E+F I ++QVV GIN KI G +YIH+K+F L
Sbjct: 36 EEFTAIEYKSQVVAGINYYIKIQTGDNRYIHIKVFKSL 73
>sp|Q28987|CYTA8_PIG Cystatin A8 (Stefin A8)
Length = 103
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDE 157
EKFE I R+QVV G N K+ +G Y+H+ +F L +E
Sbjct: 41 EKFEAIIYRSQVVAGTNYYIKVHVGGNSYVHIIVFQNLPQLNE 83
>sp|Q28986|CYTA5_PIG Cystatin A5 (Stefin A5)
Length = 103
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDE 157
EKFE I R+QVV G N K+ +G Y+H+ +F L +E
Sbjct: 41 EKFEAIIYRSQVVAGTNYYIKVHVGGNSYVHIIVFQNLPQLNE 83
>sp|P56567|CYT4_MOUSE Stefin homolog
Length = 97
Score = 38.1 bits (87), Expect = 0.006
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDEVQL 166
E F+ + +TQVV G+N K+ +G + H+K+F L K+ ++L
Sbjct: 36 EIFKAVEYKTQVVAGVNYFIKMDVGGGCFTHIKVFKDLSGKNNLEL 81
>sp|P35174|CYT2_MOUSE Stefin 2
Length = 103
Score = 38.1 bits (87), Expect = 0.006
Identities = 17/59 (28%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
Frame = +2
Query: 29 EKFEIISVRTQVVNGINMLAKIMIGSQQYIHVKIFVKLYNKDEVQL--MQCKIASKDEI 199
EKF+ I + QVV G+N K+ +G Y+H+ + + ++++++L + K + DE+
Sbjct: 42 EKFKAIEYKVQVVQGLNYFIKMNVGRGCYLHINVLSGISSENDLELTGYKAKQTNNDEL 100
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,412,174
Number of Sequences: 369166
Number of extensions: 311439
Number of successful extensions: 809
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of database: 68,354,980
effective HSP length: 44
effective length of database: 60,226,640
effective search space used: 1686345920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)