Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_L19
(851 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATP... 247 3e-65
sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATP... 237 3e-62
sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATP... 231 2e-60
sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATP... 229 6e-60
sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATP... 214 2e-55
sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATP... 206 7e-53
sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATP... 163 6e-40
sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATP... 162 8e-40
sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATP... 150 3e-36
sp|O23948|VATE_GOSHI Vacuolar ATP synthase subunit E (V-ATP... 150 4e-36
>sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 247 bits (630), Expect = 3e-65
Identities = 125/226 (55%), Positives = 168/226 (74%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
MAL++ DVQK+IK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
EK++E +KKIQ SNM+NQARLK+L +++HV VL +A+KRL +T + +Y+ L +LI
Sbjct: 61 EKQVELQKKIQSSNMLNQARLKVLKVREDHVSSVLDDARKRLGEVTKNQSEYETVLTKLI 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
QGLFQ++E KVI+RC D+ LV +L Y+A+ VEL I++ + L D GG
Sbjct: 121 VQGLFQIMEPKVILRCREVDVPLVRNVLPAAVEQYKAQINQNVELFIDEKDFLSADTCGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
VEL NGR+K+ NTLESRLDLISQQL+PEIR LFG+N NR F D
Sbjct: 181 VELLALNGRIKVPNTLESRLDLISQQLVPEIRNALFGRNVNRKFTD 226
>sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 237 bits (605), Expect = 3e-62
Identities = 123/226 (54%), Positives = 165/226 (73%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
MAL++ DVQK+IK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
EK++E +KKIQ SNM+NQARLK+L +++HV++VL EA+KRL + D + Y + L LI
Sbjct: 61 EKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDIKLYSDLLVTLI 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
Q LFQL+E V +R + D LVE +L Y+AK + V L I+ +N LP D GG
Sbjct: 121 VQALFQLVEPTVTLRVRQADKALVESLLGRAQQDYKAKIKKDVVLKIDNENFLPPDTCGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
+EL GR+KI NTLESRL+LI+QQL+PEIR LFG+N NR F D
Sbjct: 181 IELIAAKGRIKISNTLESRLELIAQQLLPEIRNALFGRNPNRKFTD 226
>sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 231 bits (589), Expect = 2e-60
Identities = 114/226 (50%), Positives = 170/226 (75%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
MAL++ DVQK+IK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+
Sbjct: 61 EKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLV 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I++++ LP+D+AGG
Sbjct: 121 LQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
VE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 VEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 229 bits (585), Expect = 6e-60
Identities = 113/226 (50%), Positives = 169/226 (74%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
MAL++ DVQK+IK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+
Sbjct: 61 EKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLV 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I+++ LP+++AGG
Sbjct: 121 LQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPVYKVATKRDVDVQIDQEAYLPEEIAGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
VE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 VEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 214 bits (545), Expect = 2e-55
Identities = 105/226 (46%), Positives = 163/226 (72%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
M ++++DVQK++++M++FIEQEA EK +EI++KA+EEF IEK +LVQ QR K+ E++ K+
Sbjct: 1 MGISDNDVQKQLRHMMAFIEQEANEKAEEIDAKAEEEFNIEKGKLVQLQRQKIMEYYEKK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
EK++E ++KIQ SNM NQ+RLK L + +H+K+VL EA+ L ++ D ++Y LK L+
Sbjct: 61 EKQVELQRKIQRSNMQNQSRLKCLKARDDHLKNVLEEARANLSKISADRERYPAILKGLL 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
QGLFQLLE+KV++RC + D +V IL EC + + E+ I+ ++ L + AGG
Sbjct: 121 LQGLFQLLESKVVLRCRKKDEEMVARILPECLEEVQRTWGNRSEVKIDNEHFLSPESAGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
VEL +G++++ +TLE+RLDLI+ ++ P++R LFG N NR F D
Sbjct: 181 VELLAKDGKIRVSSTLEARLDLIADKITPQVRTALFGPNQNRRFFD 226
>sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 228
Score = 206 bits (524), Expect = 7e-53
Identities = 107/228 (46%), Positives = 164/228 (71%), Gaps = 2/228 (0%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDE-INSKADEEFEIEKSRLVQDQRSKMNEFFSK 209
M L + DVQK+IK+M++FIEQEA EK +E I++KA+EEF IEK RL++ QR K+ E++ K
Sbjct: 1 MGLRHADVQKQIKHMMAFIEQEANEKAEEEIDAKAEEEFNIEKGRLLETQRLKIMEYYEK 60
Query: 210 EEKKL-EQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKR 386
+EK++ +Q+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L
Sbjct: 61 KEKQIRQQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLMKVVKDTTRYQVLLDG 120
Query: 387 LITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLA 566
L+ QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I+++ ++A
Sbjct: 121 LVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKRDVDVQIDQEPTCLRNIA 180
Query: 567 GGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
GGVE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 GGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 228
>sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 163 bits (412), Expect = 6e-40
Identities = 84/226 (37%), Positives = 142/226 (62%)
Frame = +3
Query: 33 MALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 212
MAL+++ V+ + M +FIE+EAKEK EI KADEE+EIEK+ +V+ + + ++ + ++
Sbjct: 1 MALSDEQVKSELSKMQAFIEKEAKEKAKEIKLKADEEYEIEKASIVRSETAAIDSTYEQK 60
Query: 213 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 392
KK ++I S + N+ RL+IL + E + ++ EA+ L +T D ++YK L LI
Sbjct: 61 LKKASLAQQITKSTIGNKTRLRILSTKDEVLHEIFDEAEAELKKITKDKKQYKPVLVGLI 120
Query: 393 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 572
+G+ L+E KV I+ D+ + + + E A +E K + KVE++I+ N L D+AGG
Sbjct: 121 EEGVLALMEPKVSIKVREQDVDVAKEAITEAAKNFEEKAKFKVEISIDDKNFLAKDIAGG 180
Query: 573 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
+ + +G++++ NTLE RL ++S++ +P IR LFG +T R F D
Sbjct: 181 IVVVNGSGKIEVDNTLEERLKILSEEALPAIRLELFGPSTTRKFFD 226
>sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 26 kDa subunit)
Length = 230
Score = 162 bits (411), Expect = 8e-40
Identities = 83/225 (36%), Positives = 141/225 (62%)
Frame = +3
Query: 36 ALTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEE 215
AL++D V + ++ M +FI+QEA+EK EI KADEEF IEKS+LV+ + ++ ++K+
Sbjct: 6 ALSDDQVGQELRKMTAFIKQEAEEKAREIQIKADEEFAIEKSKLVRQETDAIDSAYAKKF 65
Query: 216 KKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLIT 395
K+ + ++I S M N+ RL++LG +QE + ++ A +L T+D +YK+ L+ LI
Sbjct: 66 KQAQMSQQITRSTMANKTRLRVLGARQELLDEIFEAASAQLGQATHDLGRYKDILRDLIL 125
Query: 396 QGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGV 575
+G + + E +++IR + D V ++ Y+ KT+ V+ TI+ +N +P+ AGG+
Sbjct: 126 EGFYAMNEPELVIRARQADYDAVREAAGWASAQYKHKTDKDVKATIDAENPVPEGSAGGI 185
Query: 576 ELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 710
+ NG++ I NT E+RL L+ +P +R+ LFG+N NR F D
Sbjct: 186 IIVGGNGKIDIDNTFEARLTLLKDSALPAMRKALFGENPNRKFFD 230
>sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 230
Score = 150 bits (380), Expect = 3e-36
Identities = 84/229 (36%), Positives = 137/229 (59%), Gaps = 11/229 (4%)
Frame = +3
Query: 39 LTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEK 218
+ + DV ++I+ M+ FI QEA+EK +EI+ A+EEF IEK +LV+ ++ K+ + + K+EK
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEK 60
Query: 219 KLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQ 398
+ + RKKI +S +N +R+K+L Q + V + +A K L N++ D YK+ LK LI Q
Sbjct: 61 QADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQ 120
Query: 399 GLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKV-ELTINKDNCLPD------ 557
L +L E V++RC D+ LVE +LD+ Y K ++ E+ ++ LP
Sbjct: 121 CLLRLKEPSVLLRCREEDLGLVEAVLDDAKEEYAGKAKVHAPEVAVDTKIFLPPPPKSND 180
Query: 558 ----DLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNT 692
+GGV L +G++ NTL++RLD+ + +P IR+ LFG+ T
Sbjct: 181 PHGLHCSGGVVLASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFGQVT 229
>sp|O23948|VATE_GOSHI Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 237
Score = 150 bits (379), Expect = 4e-36
Identities = 85/234 (36%), Positives = 136/234 (58%), Gaps = 18/234 (7%)
Frame = +3
Query: 39 LTNDDVQKRIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEK 218
+++ DV K+I+ M+ FI QEA+EK +EI+ A+EEF IEK +LV+ ++ K+ + + K+EK
Sbjct: 1 MSDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
Query: 219 KLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQ 398
++E RKKI++S +N +R+K+L Q + V + A K N+++D YK LK LI Q
Sbjct: 61 QVEIRKKIEYSMQLNASRIKVLQAQDDVVNAMKESASKDFLNVSHDHHVYKRLLKDLIVQ 120
Query: 399 GLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDD------ 560
L +L E V++RC + D+ LVE +LD Y +K + I D LP
Sbjct: 121 SLVRLKEPGVLLRCRKEDLHLVESVLDSAKEEYASKVNVHPPEIIVDDVHLPPGPSHHHG 180
Query: 561 ------------LAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGK 686
+GGV + +G++ NTL++RLD+ + +PEIR+ LFG+
Sbjct: 181 FFHHHAEAHGPFCSGGVVIASRDGKIVFENTLDARLDVAFNKKLPEIRKWLFGQ 234
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,616,557
Number of Sequences: 369166
Number of extensions: 1677549
Number of successful extensions: 6565
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6461
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8390082510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)