Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_L14
(833 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32634|PMD1_YEAST Negative regulator of sporulation PMD1 36 0.15
sp|P07661|CIT1_ECOLI Citrate-proton symporter (Citrate tran... 33 0.97
sp|Q4L6H9|GPDA_STAHJ Glycerol-3-phosphate dehydrogenase [NA... 33 0.97
sp|P13017|AMPE_ECOLI Protein ampE 33 1.3
sp|P29086|YSO2_ACIAM Hypothetical transport protein in sor ... 32 2.2
sp|P54423|WPRA_BACSU Cell wall-associated protease precurso... 32 2.2
sp|P60363|HPPA1_RHOPA Pyrophosphate-energized proton pump (... 31 4.8
sp|P25613|YCQ0_YEAST Hypothetical 30.7 kDa protein in RVS16... 30 6.3
sp|Q89K83|HPPA_BRAJA Pyrophosphate-energized proton pump (P... 30 8.2
sp|Q8YRK5|CHLB_ANASP Light-independent protochlorophyllide ... 30 8.2
>sp|P32634|PMD1_YEAST Negative regulator of sporulation PMD1
Length = 1753
Score = 35.8 bits (81), Expect = 0.15
Identities = 26/105 (24%), Positives = 45/105 (42%)
Frame = +1
Query: 61 SNIARGYENESHTYTFQRPAVIDMSDISENSSESVKADNTKKSASDILLSKLTSTVWAES 240
S ++ GY N S Y F +S +S++ K N+ +S++ L + S
Sbjct: 618 SLLSNGYANTSFNYEFNGDTSNIISFSPHTASKTTKFGNSSQSSNGSLEKYFSKN--GNS 675
Query: 241 KERAETVFKSYASVDYLRPYFNVEPKAVLHRLLSAFIPVNIKDSD 375
K + T K SVD+ + + +A+ H LS P+ D +
Sbjct: 676 KSNSNTSLKKPHSVDFTSSTSSPKQRAISHNKLSPSEPILCADEE 720
>sp|P07661|CIT1_ECOLI Citrate-proton symporter (Citrate transporter) (Citrate carrier
protein) (Citrate utilization determinant) (Citrate
utilization protein A)
Length = 431
Score = 33.1 bits (74), Expect = 0.97
Identities = 33/125 (26%), Positives = 56/125 (44%), Gaps = 10/125 (8%)
Frame = +1
Query: 403 PVMICLTLIALLL-FEMKTSGTKVQEGTLMGTAILTCFGYWLGVSAIMWVLAYVGNTKLR 579
PV++ +TL+AL+ + T + T M T +L F ++ G+ V A T++
Sbjct: 302 PVLMGITLLALVTTLPVMNWLTAAPDFTRM-TLVLLWFSFFFGMYNGAMVAAL---TEVM 357
Query: 580 PVQINTVMGYALTGHCIVVLLGSLIHF---------GNSHVPFYIVWVICGGLCSARVVT 732
PV + TV G++L + G L G+ P + W++C LC T
Sbjct: 358 PVYVRTV-GFSLAFSLATAIFGGLTPAISTALVQLTGDKSSPGW--WLMCAALCGLAATT 414
Query: 733 IFWAR 747
+ +AR
Sbjct: 415 MLFAR 419
>sp|Q4L6H9|GPDA_STAHJ Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent
glycerol-3-phosphate dehydrogenase)
Length = 332
Score = 33.1 bits (74), Expect = 0.97
Identities = 19/81 (23%), Positives = 40/81 (49%)
Frame = +1
Query: 64 NIARGYENESHTYTFQRPAVIDMSDISENSSESVKADNTKKSASDILLSKLTSTVWAESK 243
++A+G EN+ TF+R + + ISE+ + + + A ++++ + T+ +
Sbjct: 103 HVAKGIEND----TFKRVSEMIEDSISEDHNGGIGVLSGPSHAEEVVIKQPTTVAASSKD 158
Query: 244 ERAETVFKSYASVDYLRPYFN 306
E+ + + DYLR Y N
Sbjct: 159 EKVSKLIQDLFMNDYLRVYTN 179
>sp|P13017|AMPE_ECOLI Protein ampE
Length = 284
Score = 32.7 bits (73), Expect = 1.3
Identities = 33/118 (27%), Positives = 47/118 (39%), Gaps = 12/118 (10%)
Frame = +1
Query: 481 TLMGTAILTCFGYWL------------GVSAIMWVLAYVGNTKLRPVQINTVMGYALTGH 624
TLMG A L + YWL G+ A++ VL +V PV++ V+ YAL GH
Sbjct: 161 TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWV------PVRLAGVV-YALIGH 213
Query: 625 CIVVLLGSLIHFGNSHVPFYIVWVICGGLCSARVVTIFWARTPGHSERIISAITIVVL 798
L G+ H Y V AR + TP + + + VV+
Sbjct: 214 GEKALPAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVV 271
>sp|P29086|YSO2_ACIAM Hypothetical transport protein in sor 3'region (ORF2)
Length = 253
Score = 32.0 bits (71), Expect = 2.2
Identities = 34/113 (30%), Positives = 51/113 (45%), Gaps = 12/113 (10%)
Frame = +1
Query: 397 YGPVMICLTLIALLLFEMKTSGTKVQEGTLMGTAILTCFGYWLGV------------SAI 540
Y +I + L L+L E KT+ T+V G L+G + + + LG+ +A
Sbjct: 59 YTMPLIAIPLSTLILRE-KTTKTEVI-GILIGFSGVVIYSLNLGIYFSLIGIVLTLINAF 116
Query: 541 MWVLAYVGNTKLRPVQINTVMGYALTGHCIVVLLGSLIHFGNSHVPFYIVWVI 699
W L V KLR +V + + +LLGSLI F S + FY + I
Sbjct: 117 FWALFTVYFRKLRGFDATSV-------NAVQLLLGSLIFFTLSPIQFYFKYSI 162
>sp|P54423|WPRA_BACSU Cell wall-associated protease precursor [Contains: Cell
wall-associated polypeptide CWBP23; Cell wall-associated
polypeptide CWBP52]
Length = 894
Score = 32.0 bits (71), Expect = 2.2
Identities = 25/99 (25%), Positives = 49/99 (49%)
Frame = +1
Query: 4 SYRNLYKSYMMSSTNEDNVSNIARGYENESHTYTFQRPAVIDMSDISENSSESVKADNTK 183
S N K + S+ ++ ++SN+ + + + Y+F+ P D +N + + A K
Sbjct: 335 SVHNEMKGFSASAQSKKDISNVKKAKKLFDNLYSFELP-----KDEKQNGAYTASAKRVK 389
Query: 184 KSASDILLSKLTSTVWAESKERAETVFKSYASVDYLRPY 300
+A+ LSK+++ +AE + +KS A+ D PY
Sbjct: 390 SAAA--TLSKMSNVEFAEPVQE----YKSLAN-DIQYPY 421
>sp|P60363|HPPA1_RHOPA Pyrophosphate-energized proton pump (Pyrophosphate-energized
inorganic pyrophosphatase) (H+-PPase) (Membrane-bound
proton-translocating pyrophosphatase)
Length = 706
Score = 30.8 bits (68), Expect = 4.8
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 1/84 (1%)
Frame = +1
Query: 490 GTAILTCFGYWLGVSA-IMWVLAYVGNTKLRPVQINTVMGYALTGHCIVVLLGSLIHFGN 666
G A+ C L V+ I+W+ Y T RPV+ ++ ++TGH V+ G I +
Sbjct: 330 GLALFECGIVGLAVTGLIIWITEYYTGTDFRPVK--SIAQASVTGHGTNVIQGLAISMES 387
Query: 667 SHVPFYIVWVICGGLCSARVVTIF 738
+ +P + +I G L + + +F
Sbjct: 388 TALPAIV--IIAGILITYSLAGLF 409
>sp|P25613|YCQ0_YEAST Hypothetical 30.7 kDa protein in RVS161-ADP1 intergenic region
Length = 283
Score = 30.4 bits (67), Expect = 6.3
Identities = 32/121 (26%), Positives = 54/121 (44%), Gaps = 7/121 (5%)
Frame = +1
Query: 358 NIKDSDIPVPSELYGPVMICLTLIALL--LFEMKTSGTKVQEGTLMGTAILTCFGYWLGV 531
N + I VP+ + G M L+ L+ ++E+ E T GTA+ + G+WL
Sbjct: 109 NARAQGITVPNVVVGCAMFYGGLVQLIAGIWEIAL------ENTFGGTALCSYGGFWLSF 162
Query: 532 SAIM--W---VLAYVGNTKLRPVQINTVMGYALTGHCIVVLLGSLIHFGNSHVPFYIVWV 696
+AI W + AY N +N +G+ L G I G + S V F++++
Sbjct: 163 AAIYIPWFGILEAYEDNES----DLNNALGFYLLGWAIFT-FGLTVCTMKSTVMFFLLFF 217
Query: 697 I 699
+
Sbjct: 218 L 218
>sp|Q89K83|HPPA_BRAJA Pyrophosphate-energized proton pump (Pyrophosphate-energized
inorganic pyrophosphatase) (H+-PPase) (Membrane-bound
proton-translocating pyrophosphatase)
Length = 706
Score = 30.0 bits (66), Expect = 8.2
Identities = 28/111 (25%), Positives = 54/111 (48%), Gaps = 1/111 (0%)
Frame = +1
Query: 409 MICLTLIALLLFEMKTSGTKVQEGTLMGTAILTCFGYWLGVSA-IMWVLAYVGNTKLRPV 585
++ L IA++++ + G K+ G ++ C L V+A I+W+ Y T RPV
Sbjct: 302 VLSLIGIAVVIYTLIGFG-KLDGVDYTGMSLFECGVVGLIVTALIIWITEYYTGTDYRPV 360
Query: 586 QINTVMGYALTGHCIVVLLGSLIHFGNSHVPFYIVWVICGGLCSARVVTIF 738
+ ++ ++TGH V+ G I + +P + +I G L + + +F
Sbjct: 361 K--SIAAASVTGHGTNVIQGLAISMEATALPAIV--IIAGILVTYSLAGLF 407
>sp|Q8YRK5|CHLB_ANASP Light-independent protochlorophyllide reductase subunit B (LI-POR
subunit B) (DPOR subunit B)
Length = 508
Score = 30.0 bits (66), Expect = 8.2
Identities = 31/138 (22%), Positives = 60/138 (43%), Gaps = 12/138 (8%)
Frame = +1
Query: 4 SYRNLYKSYMMSSTNEDNVSNIARGYENESHTYTFQRPAVIDMSDISENSSESVKADNTK 183
S++N++ +M + D+ N+ R + +T +V+D ++ S E V + T+
Sbjct: 22 SFKNVHA--IMHAPLGDDYFNVMRSMLSRERDFTPVTTSVVDRHVLARGSQEKVVDNITR 79
Query: 184 KSASD-----ILLSKLTSTVWAES----KERAETVFKS---YASVDYLRPYFNVEPKAVL 327
K A + +L TS++ E ERA+ K A V++ R L
Sbjct: 80 KDAEERPDLIVLTPTCTSSILQEDLENFVERAQLEAKGDVLLADVNHYRVNELQAGDRTL 139
Query: 328 HRLLSAFIPVNIKDSDIP 381
H+++ +I K ++P
Sbjct: 140 HQIVQYYIEKARKKGELP 157
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,102,366
Number of Sequences: 369166
Number of extensions: 1837822
Number of successful extensions: 4806
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4806
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)