Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_012_L11 (847 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (C... 360 2e-99 sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Cl... 350 4e-96 sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (C... 234 2e-61 sp|Q09718|AP50_SCHPO Probable clathrin coat assembly protei... 179 7e-45 sp|Q9Y6Q5|AP1M2_HUMAN Adaptor-related protein complex 1, mu... 157 3e-38 sp|Q9WVP1|AP1M2_MOUSE Adaptor-related protein complex 1, mu... 156 6e-38 sp|Q99186|APM4_YEAST Adaptin medium chain homolog APM4 155 1e-37 sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu... 153 5e-37 sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (C... 153 7e-37 sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu... 152 9e-37
>sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) (Dumpy protein 23) Length = 441 Score = 360 bits (925), Expect = 2e-99 Identities = 172/235 (73%), Positives = 200/235 (85%), Gaps = 1/235 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 +LSGMP+CKFG NDK+++E K++ DDCQFHQCVKL +FETEH Sbjct: 209 YLSGMPECKFGINDKITIEGKSKPGSDDPNKASRAAVAI--DDCQFHQCVKLTKFETEHA 266 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDGE+ELMRYRTTK+I LPFRVIPLVRE+ +++MEVK+VVK+NFKP+ AQK+EV Sbjct: 267 ISFIPPDGEYELMRYRTTKDIQLPFRVIPLVREVSRNKMEVKVVVKSNFKPSLLAQKLEV 326 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGD-RQKR 538 RIPTP NTSGVQ+ICMKGKAKYKA ENAIVWKIKRMAGMK+ Q+SAEIDLL+ G+ +K+ Sbjct: 327 RIPTPPNTSGVQLICMKGKAKYKAGENAIVWKIKRMAGMKESQISAEIDLLSTGNVEKKK 386 Query: 539 WTRPPISMNFEVPFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETRC 703 W RPP+SMNFEVPFAPSG KVRYLKVFE KLNYSDH+VIKWVRYIG+SGLYETRC Sbjct: 387 WNRPPVSMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETRC 441
>sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) (AP-2 mu 2 chain) sp|P84091|AP2M1_MOUSE Clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) (AP-2 mu 2 chain) sp|Q96CW1|AP2M1_HUMAN Clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (HA2 50 kDa subunit) (Clathrin assembly protein complex 2 medium chain) (AP-2 mu 2 chain) Length = 435 Score = 350 bits (897), Expect = 4e-96 Identities = 166/234 (70%), Positives = 197/234 (84%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 +LSGMP+CKFG NDK+ +E + + DDC FHQCV+L +F++E + Sbjct: 205 YLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAI--DDCTFHQCVRLSKFDSERS 262 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDGEFELMRYRTTK+I LPFRVIPLVRE+G++++EVK+V+K+NFKP+ AQK+EV Sbjct: 263 ISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEV 322 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 RIPTP NTSGVQVICMKGKAKYKA+ENAIVWKIKRMAGMK+ Q+SAEI+LL D+ K+W Sbjct: 323 RIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDK-KKW 381 Query: 542 TRPPISMNFEVPFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETRC 703 RPPISMNFEVPFAPSG KVRYLKVFE KLNYSDH+VIKWVRYIG+SG+YETRC Sbjct: 382 ARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC 435
>sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) Length = 439 Score = 234 bits (598), Expect = 2e-61 Identities = 119/236 (50%), Positives = 162/236 (68%), Gaps = 3/236 (1%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLE-SKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEH 178 FLSGMP+CKFG NDK+ ++ K+ + DD FHQCV+LG+F+++ Sbjct: 207 FLSGMPECKFGMNDKVIMDREKSTNGGSAARSGRRRANGIEIDDITFHQCVRLGKFDSDR 266 Query: 179 TISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVE 358 T+SFIPPDGEFELMRYRTT+ INLPF+VIP+VREMG++R+E + VK+NF F V+ Sbjct: 267 TVSFIPPDGEFELMRYRTTEHINLPFKVIPIVREMGRTRLECSVTVKSNFSSKMFGANVK 326 Query: 359 VRIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLL-TVGDRQK 535 V IPTP NT+ +++ GKAKY ++AI+W+I+R G + L AE++L+ +V +K Sbjct: 327 VIIPTPKNTAVCKIVVAAGKAKYMPEQDAIIWRIRRFPGDTEFTLRAEVELMASVNLDKK 386 Query: 536 RWTRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 W+RPPISM F+V F SGF VR+LKV E K NY+ IKWVRY+ K+G Y+ R Sbjct: 387 AWSRPPISMEFQVTMFTASGFSVRFLKVVE-KSNYTP---IKWVRYLTKAGTYQNR 438
>sp|Q09718|AP50_SCHPO Probable clathrin coat assembly protein AP50 (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) Length = 446 Score = 179 bits (455), Expect = 7e-45 Identities = 97/238 (40%), Positives = 143/238 (60%), Gaps = 6/238 (2%) Frame = +2 Query: 5 LSGMPDCKFGFNDKLSL-----ESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFE 169 LSGMP+C+FG NDKL ESK++ + +DCQFHQCV+L FE Sbjct: 213 LSGMPECQFGLNDKLDFKLKQSESKSKSNNSRNPSSVNGGFVIL-EDCQFHQCVRLPEFE 271 Query: 170 TEHTISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQ 349 EH I+FIPPDGE ELM YR+ + IN+PFR++P+V ++ K ++ ++ ++A++ P+ + Sbjct: 272 NEHRITFIPPDGEVELMSYRSHENINIPFRIVPIVEQLSKQKIIYRISIRADY-PHKLSS 330 Query: 350 KVEVRIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDR 529 + RIP PTN +GKA Y+ +EN I WKI R G + AE++L Sbjct: 331 SLNFRIPVPTNVVKANPRVNRGKAGYEPSENIINWKIPRFLGETELIFYAEVELSNT-TN 389 Query: 530 QKRWTRPPISMNFEV-PFAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 Q+ W +PPIS++F + F SG V+YL+V S+ + S ++ IKWVRY ++G E R Sbjct: 390 QQIWAKPPISLDFNILMFTSSGLHVQYLRV--SEPSNSKYKSIKWVRYSTRAGTCEIR 445
>sp|Q9Y6Q5|AP1M2_HUMAN Adaptor-related protein complex 1, mu 2 subunit (Mu-adaptin 2) (Adaptor protein complex AP-1 mu-2 subunit) (Golgi adaptor HA1/AP1 adaptin mu-2 subunit) (Clathrin assembly protein assembly protein complex 1 medium chain 2) (AP-mu chain family member mu1B) Length = 423 Score = 157 bits (397), Expect = 3e-38 Identities = 84/234 (35%), Positives = 128/234 (54%), Gaps = 1/234 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 FLSGMP+ + G ND++ E R +D +FHQCV+L RF+ + T Sbjct: 203 FLSGMPELRLGLNDRVLFELTGRSKNKSVEL----------EDVKFHQCVRLSRFDNDRT 252 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDG+FELM YR + ++ + ++ + SR+E+ + K FK A VE+ Sbjct: 253 ISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEI 312 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 +P P++ + G AKY N ++W IK G K+ + A L +V ++++ Sbjct: 313 SVPVPSDADSPRFKTSVGSAKYVPERNVVIWSIKSFPGGKEYLMRAHFGLPSV-EKEEVE 371 Query: 542 TRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 RPPI + FE+P F SG +VRY+K+ E S ++ + WVRYI +SG Y+ R Sbjct: 372 GRPPIGVKFEIPYFTVSGIQVRYMKIIEK----SGYQALPWVRYITQSGDYQLR 421
>sp|Q9WVP1|AP1M2_MOUSE Adaptor-related protein complex 1, mu 2 subunit (Mu-adaptin 2) (Adaptor protein complex AP-1 mu-2 subunit) (Golgi adaptor HA1/AP1 adaptin mu-2 subunit) (Clathrin assembly protein assembly protein complex 1 medium chain 2) (AP-mu chain family member mu1B) Length = 423 Score = 156 bits (395), Expect = 6e-38 Identities = 84/234 (35%), Positives = 128/234 (54%), Gaps = 1/234 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 FLSGMP+ + G ND++ E R +D +FHQCV+L RF+ + T Sbjct: 203 FLSGMPELRLGLNDRVLFELTGRSKNKSVEL----------EDVKFHQCVRLSRFDNDRT 252 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDG+FELM YR + ++ + ++ + SR+E+ + K FK A VE+ Sbjct: 253 ISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEI 312 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 +P P++ + G AKY +N ++W IK G K+ + A L +V + ++ Sbjct: 313 SVPVPSDADSPRFKTSVGSAKYVPEKNVVIWSIKSFPGGKEYLMRAHFGLPSV-ETEEVE 371 Query: 542 TRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 RPPI + FE+P F SG +VRY+K+ E S ++ + WVRYI +SG Y+ R Sbjct: 372 GRPPIGVKFEIPYFTVSGIQVRYMKIIEK----SGYQALPWVRYITQSGDYQLR 421
>sp|Q99186|APM4_YEAST Adaptin medium chain homolog APM4 Length = 491 Score = 155 bits (392), Expect = 1e-37 Identities = 86/250 (34%), Positives = 130/250 (52%), Gaps = 18/250 (7%) Frame = +2 Query: 5 LSGMPDCKFGFNDKLSLESKNR-------------DDXXXXXXXXXXXXXXXXDDCQFHQ 145 LSG P C+FG ND L ++S++ D +DC+FH+ Sbjct: 245 LSGTPICRFGLNDSLGMQSEDEKKWLAQQQRHSGSDFGNKNFIPKAAAGSVLLEDCKFHE 304 Query: 146 CVKLGRFETEHTISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGK-SRMEVKLVVKA 322 CV L +F H I F+PPDG ELM+Y INLPF+V P+V + + ++ ++ +K+ Sbjct: 305 CVSLDKFNRNHIIEFVPPDGSMELMKYHVRDNINLPFKVTPIVTHSTRDNEIDYRITLKS 364 Query: 323 NFKPNFFAQKVEVRIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAE 502 F A+ V + IP P +T ++ G K+ ENA++W+ + G+ + LSA Sbjct: 365 LFPGKLSAKDVVLHIPVPPSTVDCKISVSNGHCKFVPEENAMIWRFNKYNGLTENTLSA- 423 Query: 503 IDLLTVGDRQ---KRWTRPPISMNFEV-PFAPSGFKVRYLKVFESKLNYSDHEVIKWVRY 670 + + T Q ++WTRPPIS+ FEV F+ SG VRY + S H +KW++Y Sbjct: 424 VTVSTSDTTQLNLQQWTRPPISLEFEVMMFSNSGLVVRYFTISGKD---SKHRAVKWIKY 480 Query: 671 IGKSGLYETR 700 I K+G YE R Sbjct: 481 ISKAGSYEVR 490
>sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1) (Adaptor protein complex AP-1 mu-1 subunit) (Golgi adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly protein assembly protein complex 1 medium chain 1) (Clathrin coat assembly protein AP47) (Clathrin coat-associated protein AP47) (AP-mu chain family member mu1A) Length = 423 Score = 153 bits (387), Expect = 5e-37 Identities = 86/234 (36%), Positives = 125/234 (53%), Gaps = 1/234 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 FLSGMP+ + G NDK+ ++ R +D +FHQCV+L RFE + T Sbjct: 203 FLSGMPELRLGLNDKVLFDNTGRGKSKSVEL----------EDVKFHQCVRLSRFENDRT 252 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDGEFELM YR + + ++ + SR+E + K+ FK A VE+ Sbjct: 253 ISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEI 312 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 IP P + + G K+ + IVW IK G K+ + A L +V + + + Sbjct: 313 HIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSV-EAEDKE 371 Query: 542 TRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 +PPIS+ FE+P F SG +VRYLK+ E S ++ + WVRYI ++G Y+ R Sbjct: 372 GKPPISVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGDYQLR 421
>sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (Clathrin coat-associated protein AP47) (Golgi adaptor AP-1 47 kDa protein) (HA1 47 kDa subunit) (Clathrin assembly protein assembly protein complex 1 medium chain) (Uncoordinated protein 101) Length = 422 Score = 153 bits (386), Expect = 7e-37 Identities = 87/234 (37%), Positives = 123/234 (52%), Gaps = 1/234 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 +L+GMP+ + G NDK+ E R +D +FHQCV+L RF+T+ T Sbjct: 202 YLTGMPELRLGLNDKVLFEGSGRGKSKSVEL----------EDVKFHQCVRLSRFDTDRT 251 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDG FELM YR T + + + SR+ + K+ FK A VE+ Sbjct: 252 ISFIPPDGAFELMSYRLTTVVKPLIWIETSIERHSHSRVSFIIKAKSQFKRRSTANNVEI 311 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 IP P++ + G KY ++A VW IK G K+ L+A + L +V + Sbjct: 312 IIPVPSDADSPKFKTSIGSVKYTPEQSAFVWTIKNFPGGKEYLLTAHLSLPSVMSEESE- 370 Query: 542 TRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 RPPI + FE+P F SG +VRYLK+ E S ++ + WVRYI ++G YE R Sbjct: 371 GRPPIKVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGEYEMR 420
>sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1) (Adaptor protein complex AP-1 mu-1 subunit) (Golgi adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly protein assembly protein complex 1 medium chain 1) (Clathrin coat assembly protein AP47) (Clathrin coat-associated protein AP47) (AP-mu chain family member mu1A) Length = 423 Score = 152 bits (385), Expect = 9e-37 Identities = 85/234 (36%), Positives = 125/234 (53%), Gaps = 1/234 (0%) Frame = +2 Query: 2 FLSGMPDCKFGFNDKLSLESKNRDDXXXXXXXXXXXXXXXXDDCQFHQCVKLGRFETEHT 181 FLSGMP+ + G NDK+ ++ R +D +FHQCV+L RFE + T Sbjct: 203 FLSGMPELRLGLNDKVLFDNTGRGKSKSVEL----------EDVKFHQCVRLSRFENDRT 252 Query: 182 ISFIPPDGEFELMRYRTTKEINLPFRVIPLVREMGKSRMEVKLVVKANFKPNFFAQKVEV 361 ISFIPPDGEFELM YR + + ++ + SR+E + K+ FK A VE+ Sbjct: 253 ISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMVKAKSQFKRRSTANNVEI 312 Query: 362 RIPTPTNTSGVQVICMKGKAKYKAAENAIVWKIKRMAGMKDCQLSAEIDLLTVGDRQKRW 541 IP P + + G K+ + IVW +K G K+ + A L +V + + + Sbjct: 313 HIPVPNDADSPKFKTTVGSVKWVPENSEIVWSVKSFPGGKEYLMRAHFGLPSV-EAEDKE 371 Query: 542 TRPPISMNFEVP-FAPSGFKVRYLKVFESKLNYSDHEVIKWVRYIGKSGLYETR 700 +PPIS+ FE+P F SG +VRYLK+ E S ++ + WVRYI ++G Y+ R Sbjct: 372 GKPPISVKFEIPYFTTSGIQVRYLKIIEK----SGYQALPWVRYITQNGDYQLR 421
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 93,389,825 Number of Sequences: 369166 Number of extensions: 1834110 Number of successful extensions: 4899 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4634 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4845 length of database: 68,354,980 effective HSP length: 109 effective length of database: 48,218,865 effective search space used: 8293644780 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)