Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_L04
(791 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 194 3e-49
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 186 6e-47
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 143 6e-34
sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatas... 108 2e-23
sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-V... 107 5e-23
sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-... 57 8e-08
sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-... 50 9e-06
sp|Q87QK9|HIS7_VIBPA Histidine biosynthesis bifunctional pr... 43 0.001
sp|Q9KSX1|HIS7_VIBCH Histidine biosynthesis bifunctional pr... 41 0.004
sp|P08116|PVA_PLAFA Processed variable antigen 40 0.006
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 194 bits (492), Expect = 3e-49
Identities = 114/249 (45%), Positives = 151/249 (60%), Gaps = 2/249 (0%)
Frame = +2
Query: 2 EKSETVESKDDTIHEA-VETNEEKMDKFKRKLGMVKDEEEDKDWVKGNGYLVYTPSDIKS 178
E++ T ES+ DT + + +EK D K M K + W LV+T + +K
Sbjct: 107 EETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKS---NPGWENLEKLLVFTAAGVKP 163
Query: 179 SEKIASFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVIFTNQGGIE 358
K+A FDLDGTLI T+SGK F G +DW+I + EIP KL+ LE EGYKLVIFTNQ I
Sbjct: 164 QGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIG 223
Query: 359 XXXXXXXXXXXXXXAIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCD 538
A++E+L V QVLVAT ++ +YRKP TGM+D L ++ N G I+
Sbjct: 224 RGKLPAEEFKAKVEAVVEKLGVPFQVLVAT-HAGLYRKPVTGMWDHLQEQANDGTPISIG 282
Query: 539 ESLYVGDAAGRVADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFH 715
+S++VGDAAGR A+WAP + KKDFS +DRLFALN L F TPE FFL+ F++P F
Sbjct: 283 DSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFD 342
Query: 716 PAETVKNCP 742
P ++ P
Sbjct: 343 PRTVSRSGP 351
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 186 bits (472), Expect = 6e-47
Identities = 106/226 (46%), Positives = 139/226 (61%), Gaps = 1/226 (0%)
Frame = +2
Query: 44 EAVETNEEKMDKFKRKLGMVKDEEEDKDWVKGNGYLVYTPSDIKSSEKIASFDLDGTLID 223
E +T +K K LG W LV+T S +K K+A+FDLDGTLI
Sbjct: 128 EGEDTEPQKKRVRKSSLG----------WESLKKLLVFTASGVKPQGKVAAFDLDGTLIT 177
Query: 224 TKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVIFTNQGGIEXXXXXXXXXXXXXXA 403
T+SGK F +DW+I + EIP KL+ L EGYKLVIFTNQ GI A
Sbjct: 178 TRSGKVFPTSPSDWRILYPEIPKKLQELAAEGYKLVIFTNQMGIGRGKLPAEVFKGKVEA 237
Query: 404 IIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADW 583
++E+L V QVLVAT ++ + RKP +GM+D L ++ N GI I+ ++S++VGDAAGR+A+W
Sbjct: 238 VLEKLGVPFQVLVAT-HAGLNRKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAGRLANW 296
Query: 584 AP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHP 718
AP + KKDFS +DRLFALN L F TPE FFL+ F++P F P
Sbjct: 297 APGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDP 342
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 143 bits (360), Expect = 6e-34
Identities = 88/218 (40%), Positives = 124/218 (56%), Gaps = 2/218 (0%)
Frame = +2
Query: 134 KGNGYL-VYTPSDIKSSEKIASFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLE 310
K NG L ++T S+ + EKIA+FD+DGTLI TKSGK F DW++ ++ IP+ K L
Sbjct: 34 KDNGDLMIFTHSECEGKEKIAAFDMDGTLIKTKSGKVFPTNCQDWQLLYDSIPSDFKKLH 93
Query: 311 DEGYKLVIFTNQGGIEXXXXXXXXXXXXXXAIIEQLAVKIQVLVATNYSNVYRKPNTGMF 490
+G+K+VIFTNQ GI AI+ +L + +Q V+ + YRKP GM+
Sbjct: 94 SDGFKIVIFTNQKGIHAGKVDRNEFRKKIEAIVGKLGIPVQAFVSVAGGH-YRKPCVGMW 152
Query: 491 DLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAF 670
+ L K N ++IN ES++VGDAAGR+ + KKD S +DR FA N +KF TPE F
Sbjct: 153 NEL-KLRNDEVEINEKESIFVGDAAGRIKT-TSRPKKDHSYADRFFAANVGVKFQTPEEF 210
Query: 671 FLQQEEGE-FKMPEFHPAETVKNCPTLMIKYDKFQKSA 781
F + + E + P F P T + +D KS+
Sbjct: 211 FGKSKVDEPWGPPNFDPKNLFSEEITELEPHDAQLKSS 248
>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxy-kinase ]
Length = 421
Score = 108 bits (269), Expect = 2e-23
Identities = 78/235 (33%), Positives = 109/235 (46%), Gaps = 10/235 (4%)
Frame = +2
Query: 44 EAVETNEEKMDKFKRKLGMVKDEEEDKD----------WVKGNGYLVYTPSDIKSSEKIA 193
E++ + EK K +K G + + W + + +K ++K
Sbjct: 12 ESLTSYFEKSSKSSKKYGSQNKDSDSSSTCLQQKIEIQWSITDSLYIAKYGKLKKTKKFI 71
Query: 194 SFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVIFTNQGGIEXXXXX 373
+FDLDGTLI TKSG+ F++ DW + KLK L + Y LVIF+NQ GI
Sbjct: 72 AFDLDGTLIKTKSGRVFSKDAADWTWWHPSVVPKLKALYQDNYSLVIFSNQNGIPRKPSA 131
Query: 374 XXXXXXXXXAIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYV 553
AI E L + I VL A + +RKP TGM++ K+ N I ++ + YV
Sbjct: 132 GHTFQMKIRAIFESLDLPI-VLYAAILKDKFRKPLTGMWNSFLKDVNRSIDLSFIK--YV 188
Query: 554 GDAAGRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHP 718
GDAAGR D +++D FA N +KF TPE FFL FHP
Sbjct: 189 GDAAGRPG--------DHNSTDLKFAENIGIKFETPEQFFLGHSFVPPNFESFHP 235
>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
Length = 182
Score = 107 bits (266), Expect = 5e-23
Identities = 69/172 (40%), Positives = 96/172 (55%)
Frame = +2
Query: 155 YTPSDIKSSEKIASFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVI 334
Y D KIA+FDLDGTLI +K+ KF + +DW++ +KLK L + GY LV+
Sbjct: 12 YAVHDGAKRTKIAAFDLDGTLISSKTRSKFPKNPDDWQLL--PCAHKLKRLYELGYDLVV 69
Query: 335 FTNQGGIEXXXXXXXXXXXXXXAIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHN 514
FTNQ + I + V I V+ N + +RKP+TGM+ + K+
Sbjct: 70 FTNQAHLGSGKIKASDLLYKLENIKKATGVPISFYVSPN-KDEHRKPDTGMWREMAKQFT 128
Query: 515 SGIKINCDESLYVGDAAGRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAF 670
I+ ++S YVGDAAGR+ +KDFS+SDR+FA N L+FYTPE F
Sbjct: 129 ---HIDKEQSFYVGDAAGRIN--LTTGQKDFSDSDRVFAKNLSLQFYTPEQF 175
>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 238
Score = 56.6 bits (135), Expect = 8e-08
Identities = 61/214 (28%), Positives = 83/214 (38%), Gaps = 52/214 (24%)
Frame = +2
Query: 188 IASFDLDGTLIDTKSGK-KFAEGINDWK-IKFNEIPNKLKTL-----EDEGYKLVIFTNQ 346
+ +FDLD T+I KS F+ +DW+ I FN + L L D +VIF+NQ
Sbjct: 31 VYAFDLDHTIIKPKSPNISFSRSASDWQFINFNSKKSTLDYLCNIIDNDPTAVIVIFSNQ 90
Query: 347 GGIEXXXXXXXXXXXXXXAIIEQL--------------------AVKIQVLVATNYSNV- 463
GG+ I+ L A K A N+S +
Sbjct: 91 GGVITVPRTSKSCTKYTNKILLFLKAIKNDERGETLSHRLWLYAAPKRPKTFAANHSKIT 150
Query: 464 -----------------YRKPNTGMFDLLDKEHNSGIKINCDES-------LYVGDAAGR 571
RKP TGM + ++ S +++ S Y GDAAGR
Sbjct: 151 FASLGESYNNDPNIFEKVRKPMTGMVEFFKRDLESAYRVSEQISPIKLNWIYYCGDAAGR 210
Query: 572 VADWAPKMKKDFSNSDRLFALNCHLKFYTPEAFF 673
KKDFS+SD FA N H++F PE F
Sbjct: 211 --------KKDFSDSDIKFAENLHVEFKYPEEIF 236
>sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 245
Score = 49.7 bits (117), Expect = 9e-06
Identities = 58/216 (26%), Positives = 82/216 (37%), Gaps = 54/216 (25%)
Frame = +2
Query: 188 IASFDLDGTLIDTKS-GKKFAEGINDWKIKFNEIPNKLKTLE--------DEGYKLVIFT 340
+ +FDLD T+I KS K++ NDW+ F +K TL+ D +VIF+
Sbjct: 38 VYAFDLDHTIIKPKSPNAKYSRSANDWQ--FMNFDSKKSTLDYLFSITDNDLAAVIVIFS 95
Query: 341 NQGGIEXXXXXXXXXXXXXXAII------------EQLAVKIQVLVAT------------ 448
NQGG+ I E+L+ ++ + A
Sbjct: 96 NQGGVITVPRTSKSCSKYINKISLFLKAIENDKRGEKLSPRLWIYAAPKRPKTVVTNNKK 155
Query: 449 --------NYSN------VYRKPNTGMFDLLDKEHNSGIK-------INCDESLYVGDAA 565
+Y+N RKP TGM D + + I + Y GDAA
Sbjct: 156 ITFPSLCKSYNNDPEIFEKVRKPMTGMADFFRIDITDACRVLKTVPPIKLNWVYYCGDAA 215
Query: 566 GRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAFF 673
GR K DFS+SD FA H++F PE F
Sbjct: 216 GR--------KNDFSDSDIKFAEKLHVEFKYPEEVF 243
>sp|Q87QK9|HIS7_VIBPA Histidine biosynthesis bifunctional protein hisB [Includes:
Histidinol-phosphatase ; Imidazoleglycerol-phosphate
dehydratase (IGPD)]
Length = 357
Score = 42.7 bits (99), Expect = 0.001
Identities = 40/137 (29%), Positives = 61/137 (44%), Gaps = 7/137 (5%)
Frame = +2
Query: 170 IKSSEKIASFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVIFTNQG 349
+ +KI D DGTLI + ++ K++ IP+ L +L+D GY+LV+ TNQ
Sbjct: 1 MSKQQKILFIDRDGTLIVEPPIDFQVDRLDKLKLEPFVIPSLL-SLQDAGYRLVMVTNQD 59
Query: 350 GI---EXXXXXXXXXXXXXXAIIEQLAVKI-QVLVATNYSN---VYRKPNTGMFDLLDKE 508
G+ I E VK VL+ ++ RKP GM KE
Sbjct: 60 GLGTDSYPQEDFDAPHNMMMEIFESQGVKFDDVLICPHFEEDNCSCRKPKLGMV----KE 115
Query: 509 HNSGIKINCDESLYVGD 559
+ G K++ S+ +GD
Sbjct: 116 YLQGGKVDFQNSVVIGD 132
>sp|Q9KSX1|HIS7_VIBCH Histidine biosynthesis bifunctional protein hisB [Includes:
Histidinol-phosphatase ; Imidazoleglycerol-phosphate
dehydratase (IGPD)]
Length = 357
Score = 40.8 bits (94), Expect = 0.004
Identities = 40/137 (29%), Positives = 60/137 (43%), Gaps = 7/137 (5%)
Frame = +2
Query: 170 IKSSEKIASFDLDGTLIDTKSGKKFAEGINDWKIKFNEIPNKLKTLEDEGYKLVIFTNQG 349
+ +KI D DGTLI + ++ K++ IP+ LK L++ GY+LV+ TNQ
Sbjct: 1 MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLK-LQEAGYRLVMVTNQD 59
Query: 350 GI---EXXXXXXXXXXXXXXAIIEQLAVKI-QVLVATNYSN---VYRKPNTGMFDLLDKE 508
G+ I E VK VL+ ++ RKP G L KE
Sbjct: 60 GLGTSSYPQADFDAPHNMMMDIFESQGVKFNDVLICPHFERDNCSCRKPKLG----LVKE 115
Query: 509 HNSGIKINCDESLYVGD 559
+ G K++ S +GD
Sbjct: 116 YLQGGKVDFKSSAVIGD 132
>sp|P08116|PVA_PLAFA Processed variable antigen
Length = 224
Score = 40.4 bits (93), Expect = 0.006
Identities = 35/111 (31%), Positives = 49/111 (44%), Gaps = 3/111 (2%)
Frame = +2
Query: 2 EKSETVESKDDTIHEAVETNEEKMDKFKRKLGMVKDEEEDKDWVKGNGYLVYTPSDIKSS 181
E ET ESK+ I+E ET K+ R+ VK EE KD G V P + +
Sbjct: 94 ESKETGESKETRIYE--ETKYNKITSEFRETENVKITEESKD---REGNKVSGPYENSEN 148
Query: 182 EKIASFDLDGTLIDTKS---GKKFAEGINDWKIKFNEIPNKLKTLEDEGYK 325
+ S + + K G+K E +ND + +E P KL E+ G K
Sbjct: 149 SNVTSESEETKKLAEKEENEGEKLGENVNDGASENSEDPKKLTEQEENGTK 199
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,234,095
Number of Sequences: 369166
Number of extensions: 1416195
Number of successful extensions: 4988
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4960
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7425705210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)