Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_K24
(513 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35609|ACTN2_HUMAN Alpha-actinin 2 (Alpha actinin skelet... 119 4e-27
sp|P57780|ACTN4_MOUSE Alpha-actinin 4 (Non-muscle alpha-act... 118 7e-27
sp|O43707|ACTN4_HUMAN Alpha-actinin 4 (Non-muscle alpha-act... 118 9e-27
sp|Q9QXQ0|ACTN4_RAT Alpha-actinin 4 (Non-muscle alpha-actin... 118 9e-27
sp|Q9JI91|ACTN2_MOUSE Alpha-actinin 2 (Alpha actinin skelet... 115 6e-26
sp|P20111|ACTN2_CHICK Alpha-actinin 2 (Alpha actinin skelet... 115 8e-26
sp|Q9Z1P2|ACTN1_RAT Alpha-actinin 1 (Alpha-actinin cytoskel... 114 1e-25
sp|P05094|ACTN1_CHICK Alpha-actinin 1 (Alpha-actinin cytosk... 114 2e-25
sp|P12814|ACTN1_HUMAN Alpha-actinin 1 (Alpha-actinin cytosk... 113 3e-25
sp|Q7TPR4|ACTN1_MOUSE Alpha-actinin 1 (Alpha-actinin cytosk... 113 3e-25
>sp|P35609|ACTN2_HUMAN Alpha-actinin 2 (Alpha actinin skeletal muscle isoform 2) (F-actin
cross linking protein)
Length = 894
Score = 119 bits (298), Expect = 4e-27
Identities = 63/170 (37%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L++AK+ APF NW+E A EDLQD I+ I+E++ LI ++FK ++ A
Sbjct: 510 LLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEAD 569
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + ++++ N+ S NPY+ ++ L + +K+ L+P+RD+ LQ+EL
Sbjct: 570 GERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEEL 629
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q NE+LR F + AN +G WI K + I S + LE Q +Q+
Sbjct: 630 ARQHANERLRRQFAAQANAIGPWIQNKMEEI-ARSSIQITGALEDQMNQL 678
>sp|P57780|ACTN4_MOUSE Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 912
Score = 118 bits (296), Expect = 7e-27
Identities = 67/169 (39%), Positives = 97/169 (57%)
Frame = +3
Query: 6 LEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAKL 185
LE IDQ+ L+YAK+ APF NW+E A EDLQD I+ I+E++ LI D+FK ++ +A
Sbjct: 524 LETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADR 583
Query: 186 EYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKELS 365
E + + + + A N++ NPYT ++ ++ + K+ L+P RD L +E S
Sbjct: 584 EREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQS 643
Query: 366 QQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
+Q NE LR F S AN +G WI K + I IS E TLE Q S +
Sbjct: 644 KQQSNEHLRRQFASQANMVGPWIQTKMEEI-GRISIEMNGTLEDQLSHL 691
>sp|O43707|ACTN4_HUMAN Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 911
Score = 118 bits (295), Expect = 9e-27
Identities = 67/169 (39%), Positives = 97/169 (57%)
Frame = +3
Query: 6 LEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAKL 185
LE IDQ+ L+YAK+ APF NW+E A EDLQD I+ I+E++ LI D+FK ++ +A
Sbjct: 523 LEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADR 582
Query: 186 EYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKELS 365
E + + + + A N++ NPYT ++ ++ + K+ L+P RD L +E S
Sbjct: 583 EREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQS 642
Query: 366 QQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
+Q NE LR F S AN +G WI K + I IS E TLE Q S +
Sbjct: 643 KQQSNEHLRRQFASQANVVGPWIQTKMEEI-GRISIEMNGTLEDQLSHL 690
Score = 30.0 bits (66), Expect = 3.3
Identities = 31/153 (20%), Positives = 65/153 (42%), Gaps = 4/153 (2%)
Frame = +3
Query: 9 EEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAKLE 188
+E + + DY K + WI + L+D I E++ +E ++++ + K++
Sbjct: 289 QENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQ 348
Query: 189 YNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NK----LSDLLPLRDEFLQK 356
+ +I L+ L ++S P + S+ + + N L +E+L
Sbjct: 349 ----EKCQLEINFNTLQTKL-RLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLN 403
Query: 357 ELSQQTKNEKLRENFGSLANKLGAWIDEKQQFI 455
E+ + + + L E F A+ AW D K+ +
Sbjct: 404 EIRRLERLDHLAEKFRQKASIHEAWTDGKEAML 436
>sp|Q9QXQ0|ACTN4_RAT Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 911
Score = 118 bits (295), Expect = 9e-27
Identities = 67/169 (39%), Positives = 97/169 (57%)
Frame = +3
Query: 6 LEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAKL 185
LE IDQ+ L+YAK+ APF NW+E A EDLQD I+ I+E++ LI D+FK ++ +A
Sbjct: 523 LETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADR 582
Query: 186 EYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKELS 365
E + + + + A N++ NPYT ++ ++ + K+ L+P RD L +E S
Sbjct: 583 EREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQS 642
Query: 366 QQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
+Q NE LR F S AN +G WI K + I IS E TLE Q S +
Sbjct: 643 KQQSNEHLRRQFASQANMVGPWIQTKMEEI-GRISIEMNGTLEDQLSHL 690
>sp|Q9JI91|ACTN2_MOUSE Alpha-actinin 2 (Alpha actinin skeletal muscle isoform 2) (F-actin
cross linking protein)
Length = 894
Score = 115 bits (288), Expect = 6e-26
Identities = 62/170 (36%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L++AK+ APF NW+E A EDLQD I+ I+E++ LI ++FK ++ A
Sbjct: 510 LLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEAD 569
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + ++++ ++ S NPY+ ++ L + +K+ L+P+RD+ LQ+EL
Sbjct: 570 GERQSILAIQNEVEKVIQSYSIRISSSNPYSTVTMDELRNKWDKVKQLVPVRDQSLQEEL 629
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q NE+LR F + AN +G WI K + I S + LE Q +Q+
Sbjct: 630 ARQHANERLRRQFAAQANAIGPWIQNKMEEI-ARSSIQITGALEDQMNQL 678
>sp|P20111|ACTN2_CHICK Alpha-actinin 2 (Alpha actinin skeletal muscle isoform 2) (F-actin
cross linking protein)
Length = 897
Score = 115 bits (287), Expect = 8e-26
Identities = 62/170 (36%), Positives = 100/170 (58%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L++AK+ APF NW+E A EDLQD I+ I+E++ LI D+FK ++ A
Sbjct: 513 LLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLISAHDQFKATLPEAD 572
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + ++++ ++ + NPY+ ++ + + K+ L+P RD+ LQ+EL
Sbjct: 573 GERQAILSIQNEVEKVIQSYSMRISASNPYSTVTVEEIRTKWEKVKQLVPQRDQSLQEEL 632
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q NE+LR F + AN +G WI K + I S E LE Q +Q+
Sbjct: 633 ARQHANERLRRQFAAQANVIGPWIQTKMEEI-ARSSIEMTGPLEDQMNQL 681
>sp|Q9Z1P2|ACTN1_RAT Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 114 bits (285), Expect = 1e-25
Identities = 64/170 (37%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L+YAK+ APF NW+E A EDLQD+ I+ I+E++ L ++FK ++ +A
Sbjct: 503 LLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDAD 562
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + +++ ++N NPYT ++ ++ + + + L+P RD+ L +E
Sbjct: 563 KERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEH 622
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
S+Q NE+LR+ FG+ AN +G WI K + I IS E TLE Q S +
Sbjct: 623 SRQQHNERLRKQFGAQANVIGPWIQTKMEEI-GRISIEMHGTLEDQLSHL 671
>sp|P05094|ACTN1_CHICK Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 893
Score = 114 bits (284), Expect = 2e-25
Identities = 62/170 (36%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L+YAK+ APF NW+E A EDLQD+ I+ I+E++ L ++FK ++ +A
Sbjct: 504 LLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDAD 563
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + +++ ++N NPYT ++ ++ + + L+P RD+ L +E
Sbjct: 564 KERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEH 623
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q +NE+LR+ FG+ AN +G WI K + I IS E TLE Q + +
Sbjct: 624 ARQQQNERLRKQFGAQANVIGPWIQTKMEEI-GRISIEMHGTLEDQLNHL 672
>sp|P12814|ACTN1_HUMAN Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 113 bits (282), Expect = 3e-25
Identities = 63/170 (37%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L+YAK+ APF NW+E A EDLQD+ I+ I+E++ L ++FK ++ +A
Sbjct: 503 LLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDAD 562
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + +++ ++N NPYT ++ ++ + + + L+P RD+ L +E
Sbjct: 563 KERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEH 622
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q NE+LR+ FG+ AN +G WI K + I IS E TLE Q S +
Sbjct: 623 ARQQHNERLRKQFGAQANVIGPWIQTKMEEI-GRISIEMHGTLEDQLSHL 671
>sp|Q7TPR4|ACTN1_MOUSE Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 113 bits (282), Expect = 3e-25
Identities = 63/170 (37%), Positives = 102/170 (60%)
Frame = +3
Query: 3 LLEEIDQMQLDYAKKIAPFYNWIEQAKEDLQDSHILLYIDEVKILIEKQDEFKKSMENAK 182
LLE IDQ+ L+YAK+ APF NW+E A EDLQD+ I+ I+E++ L ++FK ++ +A
Sbjct: 503 LLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDAD 562
Query: 183 LEYNMLNQVDSQIKNRALENNLNQISENPYTLLSQTALSVE*NKLSDLLPLRDEFLQKEL 362
E + + +++ ++N NPYT ++ ++ + + + L+P RD+ L +E
Sbjct: 563 KERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEH 622
Query: 363 SQQTKNEKLRENFGSLANKLGAWIDEKQQFIYYLISGENVSTLESQKSQI 512
++Q NE+LR+ FG+ AN +G WI K + I IS E TLE Q S +
Sbjct: 623 ARQQHNERLRKQFGAQANVIGPWIQTKMEEI-GRISIEMHGTLEDQLSHL 671
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,059,199
Number of Sequences: 369166
Number of extensions: 820428
Number of successful extensions: 3268
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3258
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)