Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_K22
(758 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 160 3e-39
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 158 1e-38
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 156 5e-38
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 155 1e-37
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 153 4e-37
sp|Q9VXE5|PAKM_DROME Serine/threonine-protein kinase PAK mb... 152 7e-37
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 150 4e-36
sp|O22932|CPK11_ARATH CBL-interacting serine/threonine-prot... 150 4e-36
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 150 4e-36
sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE... 150 5e-36
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 160 bits (406), Expect = 3e-39
Identities = 88/226 (38%), Positives = 132/226 (58%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
KT+ +E E+Q+ K+ HP IV+Y + D++ ++ME++ GGS+ + K G
Sbjct: 110 KTQGHIRELEEEVQLL-KNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSF 168
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E I Y+KQ+L L+YLH I+HRDIK NIL+D ++L DFG SK + +LA
Sbjct: 169 PEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELAT-- 226
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+NGA +S GTP +MAPE++ + + SAD+WS G T++EM TG PP+ + +
Sbjct: 227 -VNGA-KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVL 284
Query: 543 ISESRKIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFL 680
K P DLS K F+ CL EP+ RL++ +L+ HPF+
Sbjct: 285 HIGRTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 158 bits (400), Expect = 1e-38
Identities = 85/226 (37%), Positives = 133/226 (58%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
KT+ +E E+++ K+ HP IV+Y + +D + +++E++ GGS+ + K GP
Sbjct: 111 KTQAHIQELEEEVKLL-KNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPF 169
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E+ ++ Y++Q+L L+YLH I+HRDIK NIL+D +KL DFG SK + +LA
Sbjct: 170 PESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELAT-- 227
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+ GA +S GTP +MAPE++ + + SAD+WS G T++EMVTG P+ Q
Sbjct: 228 -MTGA-KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIF 285
Query: 543 ISESRKIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFL 680
+ K P+ LS + K F+ CLQ P R T+ +L+ HPF+
Sbjct: 286 FIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 156 bits (395), Expect = 5e-38
Identities = 85/228 (37%), Positives = 137/228 (60%), Gaps = 2/228 (0%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
+ ++E +++E+ V S+ D P + KY ++ + ++++MEY+ GGS + GPL
Sbjct: 71 EAEDEIEDIQQEITVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPL 128
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E +I ++IL+ L YLH +K +HRDIK+ N+LL E VKL DFG++ LTD K
Sbjct: 129 DETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKR 188
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+ VGTP +MAPE++ + Y S AD+WS G T +E+ G PP+ ++ KV+ +
Sbjct: 189 ------NTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFL 242
Query: 543 ISESR--KIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFL 680
I ++ +E NY S LK F+++CL EP+ R T+K+L+ H F+
Sbjct: 243 IPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 286
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 155 bits (392), Expect = 1e-37
Identities = 85/228 (37%), Positives = 137/228 (60%), Gaps = 2/228 (0%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
+ ++E +++E+ V S+ D P + KY ++ + ++++MEY+ GGS + GPL
Sbjct: 59 EAEDEIEDIQQEITVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPL 116
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E +I ++IL+ L YLH +K +HRDIK+ N+LL E VKL DFG++ LTD K
Sbjct: 117 DEIQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKR 176
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+ VGTP +MAPE++ + Y S AD+WS G T +E+ G PP+ ++ KV+ +
Sbjct: 177 ------NTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPHSELHPMKVLFL 230
Query: 543 ISESR--KIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFL 680
I ++ +E NY S LK F+++CL EP+ R T+K+L+ H F+
Sbjct: 231 IPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 274
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 153 bits (387), Expect = 4e-37
Identities = 83/228 (36%), Positives = 136/228 (59%), Gaps = 2/228 (0%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
KT+ +E E+++ K+ HP IV+Y + ++ + +++E++ GGS+ + K G
Sbjct: 110 KTQAHIQELEEEVKLL-KNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAF 168
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E+ ++ Y+ Q+L L+YLH I+HRDIK NIL+D +KL DFG SK + +LA
Sbjct: 169 PESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELAT-- 226
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQM--GVCKVM 536
++GA +S GTP +MAPE++ + + SAD+WS G T++EMVTG P+ Q + +
Sbjct: 227 -ISGA-KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIF 284
Query: 537 KIISESRKIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFL 680
I + K P+++S + F+ CLQ EP R T+ +L+ HPF+
Sbjct: 285 HI--GTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
>sp|Q9VXE5|PAKM_DROME Serine/threonine-protein kinase PAK mbt (p21-activated kinase related
protein) (Mushroom bodies tiny protein)
Length = 639
Score = 152 bits (385), Expect = 7e-37
Identities = 81/207 (39%), Positives = 123/207 (59%)
Frame = +3
Query: 66 HPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEALQYLHE 245
HP IV+ +F D +++VMEY++GG+L + + + E +I KQ L+AL YLH
Sbjct: 422 HPNIVETYSSFLVNDELWVVMEYLEGGALTDIVT-HSRMDEEQIATVCKQCLKALAYLHS 480
Query: 246 QKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPNYMAPEIM 425
Q ++HRDIKSD+ILL D VKL DFG ++ K +S VGTP +M+PE++
Sbjct: 481 QGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKR------KSLVGTPYWMSPEVI 534
Query: 426 NEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKIISESRKIEYNYPNDLSDNLKS 605
+ PYG D+WS G ++EMV G PP+ + M+ I + + + +S L+S
Sbjct: 535 SRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPPNLKNAHKVSPRLQS 594
Query: 606 FIDSCLQSEPARRLTSKQLMNHPFLRK 686
F+D L +PA+R T+ +L+ HPFLR+
Sbjct: 595 FLDRMLVRDPAQRATAAELLAHPFLRQ 621
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 150 bits (379), Expect = 4e-36
Identities = 83/228 (36%), Positives = 132/228 (57%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
+ ++E +++E+ V S+ D + KY ++ ++++MEY+ GGS +R GP
Sbjct: 59 EAEDEIEDIQQEITVLSQ-CDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPF 116
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E +I K+IL+ L YLH +K +HRDIK+ N+LL E +VKL DFG++ LTD K
Sbjct: 117 DEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKR 176
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+ VGTP +MAPE++ + Y S AD+WS G T +E+ G PP M +V+ +
Sbjct: 177 ------NTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFL 230
Query: 543 ISESRKIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFLRK 686
I ++ D + + K FID+CL +P+ R T+K+L+ H F+ K
Sbjct: 231 IPKNN--PPTLVGDFTKSFKEFIDACLNKDPSFRPTAKELLKHKFIVK 276
>sp|O22932|CPK11_ARATH CBL-interacting serine/threonine-protein kinase 11 (SOS2-like
protein kinase PKS5) (SOS-interacting protein 4)
(SNF1-related kinase 3.22)
Length = 435
Score = 150 bits (379), Expect = 4e-36
Identities = 82/219 (37%), Positives = 131/219 (59%), Gaps = 1/219 (0%)
Frame = +3
Query: 24 SVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKK 203
+++RE+ + + S HP IVK T+ IF ME++KGG L I K+G LSE+ ++
Sbjct: 66 NIKREISIMRRLS-HPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRR 124
Query: 204 YSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQ 383
Y +Q++ A+ Y H + + HRD+K +N+L+DE+ N+K+ DFGLS LTD D G +
Sbjct: 125 YFQQLISAVGYCHARGVYHRDLKPENLLIDENGNLKVSDFGLS-ALTDQIRPD---GLLH 180
Query: 384 SSVGTPNYMAPEIMNEEPY-GSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKIISESRK 560
+ GTP Y+APEI++++ Y G+ DVWS G + +V G+ P++ V + K I K
Sbjct: 181 TLCGTPAYVAPEILSKKGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKI---YK 237
Query: 561 IEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPF 677
EY +P +S +LK F+ L P R+T +++ P+
Sbjct: 238 GEYRFPRWMSPDLKRFVSRLLDINPETRITIDEILKDPW 276
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 150 bits (379), Expect = 4e-36
Identities = 85/231 (36%), Positives = 134/231 (58%), Gaps = 3/231 (1%)
Frame = +3
Query: 3 KTKEEKLSVERELQVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPL 182
+ ++E +++E+ V S+ D + KY ++ ++++MEY+ GGS +R GP
Sbjct: 59 EAEDEIEDIQQEITVLSQ-CDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPF 116
Query: 183 SENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKD 362
E +I K+IL+ L YLH +K +HRDIK+ N+LL E +VKL DFG++ LTD K
Sbjct: 117 DEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKR 176
Query: 363 FLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKVMKI 542
+ VGTP +MAPE++ + Y S AD+WS G T +E+ G PP M +V+ +
Sbjct: 177 ------NTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFL 230
Query: 543 ISESRKIEYNYP---NDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFLRK 686
I ++ N P D + + K FID+CL +P+ R T+K+L+ H F+ K
Sbjct: 231 IPKN-----NPPTLIGDFTKSFKEFIDACLNKDPSFRPTAKELLKHKFIVK 276
>sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE20-like kinase MST1) (MST-1)
(Mammalian STE20-like protein kinase 1)
(Serine/threonine-protein kinase Krs-2)
Length = 487
Score = 150 bits (378), Expect = 5e-36
Identities = 86/237 (36%), Positives = 131/237 (55%), Gaps = 7/237 (2%)
Frame = +3
Query: 15 EKLSVERELQVYSKH------SDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIR-KN 173
+++ VE +LQ K D P +VKY ++ +++VMEY GS+ IR +N
Sbjct: 59 KQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRN 118
Query: 174 GPLSENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLA 353
L+E++I + L+ L+YLH + +HRDIK+ NILL+ + + KL DFG++ LTD
Sbjct: 119 KTLTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTM 178
Query: 354 AKDFLNGAIQSSVGTPNYMAPEIMNEEPYGSSADVWSFGATMVEMVTGFPPYHQMGVCKV 533
AK + +GTP +MAPE++ E Y AD+WS G T +EM G PPY + +
Sbjct: 179 AKR------NTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRA 232
Query: 534 MKIISESRKIEYNYPNDLSDNLKSFIDSCLQSEPARRLTSKQLMNHPFLRKFELVFI 704
+ +I + + P SDN F+ CL P +R T+ QL+ HPF+R + V I
Sbjct: 233 IFMIPTNPPPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSI 289
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,734,292
Number of Sequences: 369166
Number of extensions: 1924516
Number of successful extensions: 9585
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6512
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6960
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)