Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_K09
(862 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P50495|MSP1_PLAFP Merozoite surface protein 1 precursor ... 37 0.071
sp|O60237|MYPT2_HUMAN Protein phosphatase 1 regulatory subu... 36 0.12
sp|P04934|MSP1_PLAFC Merozoite surface protein 1 precursor ... 36 0.12
sp|P19598|MSP1_PLAF3 Merozoite surface protein 1 precursor ... 36 0.12
sp|P08569|MSP1_PLAFM Merozoite surface protein 1 precursor ... 35 0.35
sp|P13819|MSP1_PLAFF Merozoite surface protein 1 precursor ... 35 0.35
sp|Q9DCF1|TMM25_MOUSE Transmembrane protein 25 precursor 32 1.7
sp|Q62469|ITA2_MOUSE Integrin alpha-2 precursor (Platelet m... 32 1.7
sp|Q09388|ACM2_CAEEL Probable muscarinic acetylcholine rece... 32 3.0
sp|Q25188|OAR_HELVI Octopamine receptor 32 3.0
>sp|P50495|MSP1_PLAFP Merozoite surface protein 1 precursor (Merozoite surface antigens)
(PMMSA) (GP195)
Length = 1726
Score = 37.0 bits (84), Expect = 0.071
Identities = 43/146 (29%), Positives = 63/146 (43%), Gaps = 27/146 (18%)
Frame = +2
Query: 275 NKRKSNRNKSVMSTLS----LMKHSKGACKNQQGDSE-LQTLSCNSLRLWSSDKAKEKSV 439
NK+K +TL L+KH KG K G+S L+TLS S++ + EK
Sbjct: 1120 NKKKEAEIAETENTLENTKILLKHYKGLVKYYNGESSPLKTLSEVSIQTEDNYANLEKFR 1179
Query: 440 VLKRLLNEWNE------VQLTMASSTDYKLILE-----------GTIPSGNPNAKI---- 556
VL ++ + N+ +L+ SS ++LI E G PS N N K+
Sbjct: 1180 VLSKIDGKLNDNLHLGKKKLSFLSSGLHQLITELKEVIKNKNYTGNSPSEN-NKKVNEAL 1238
Query: 557 -CVDNITSSAEPCEIPKPPQPVAAPS 631
+N A+ + PPQP PS
Sbjct: 1239 KSYENFLPEAKVTTVVTPPQPDVTPS 1264
>sp|O60237|MYPT2_HUMAN Protein phosphatase 1 regulatory subunit 12B (Myosin phosphatase
targeting subunit 2) (Myosin phosphatase target subunit
2)
Length = 982
Score = 36.2 bits (82), Expect = 0.12
Identities = 36/130 (27%), Positives = 56/130 (43%), Gaps = 6/130 (4%)
Frame = +2
Query: 269 IDNKRKSNRNKSVMSTLSLMKHSKGA----CKNQQGDSELQTLSCNSLRLWSSDKAKEKS 436
+DNK K NKS +S+L+ K + + +N++ L + +LW D+AK
Sbjct: 481 LDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLW-RDEAK--- 536
Query: 437 VVLKRLLNEWNEVQLTMASSTDYKLILEGT--IPSGNPNAKICVDNITSSAEPCEIPKPP 610
NE+ T+A ST L+ T + A+ DN++SS C I P
Sbjct: 537 ---------GNEIPQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRP 587
Query: 611 QPVAAPSFTS 640
P A T+
Sbjct: 588 LPSTANGVTA 597
>sp|P04934|MSP1_PLAFC Merozoite surface protein 1 precursor (Merozoite surface antigens)
(PMMSA) (P195)
Length = 1726
Score = 36.2 bits (82), Expect = 0.12
Identities = 43/146 (29%), Positives = 62/146 (42%), Gaps = 27/146 (18%)
Frame = +2
Query: 275 NKRKSNRNKSVMSTLS----LMKHSKGACKNQQGDSE-LQTLSCNSLRLWSSDKAKEKSV 439
NK+K +TL L+KH KG K G+S L+TLS S++ + EK
Sbjct: 1120 NKKKEAEIAETENTLENTKILLKHYKGLVKYYNGESSPLKTLSEVSIQTEDNYANLEKFR 1179
Query: 440 VLKRLLNEWNE------VQLTMASSTDYKLILE-----------GTIPSGNPNAKI---- 556
VL ++ + N+ +L+ SS + LI E G PS N N K+
Sbjct: 1180 VLSKIDGKLNDNLHLGKKKLSFLSSGLHHLITELKEVIKNKNYTGNSPSEN-NKKVNEAL 1238
Query: 557 -CVDNITSSAEPCEIPKPPQPVAAPS 631
+N A+ + PPQP PS
Sbjct: 1239 KSYENFLPEAKVTTVVTPPQPDVTPS 1264
>sp|P19598|MSP1_PLAF3 Merozoite surface protein 1 precursor (Merozoite surface antigens)
(PMMSA) (P190)
Length = 1682
Score = 36.2 bits (82), Expect = 0.12
Identities = 43/146 (29%), Positives = 62/146 (42%), Gaps = 27/146 (18%)
Frame = +2
Query: 275 NKRKSNRNKSVMSTLS----LMKHSKGACKNQQGDSE-LQTLSCNSLRLWSSDKAKEKSV 439
NK+K +TL L+KH KG K G+S L+TLS S++ + EK
Sbjct: 1077 NKKKEAEIAETENTLENTKILLKHYKGLVKYYNGESSPLKTLSEVSIQTEDNYANLEKFR 1136
Query: 440 VLKRLLNEWNE------VQLTMASSTDYKLILE-----------GTIPSGNPNAKI---- 556
VL ++ + N+ +L+ SS + LI E G PS N N K+
Sbjct: 1137 VLSKIDGKLNDNLHLGKKKLSFLSSGLHHLITELKEVIKNKNYTGNSPSEN-NKKVNEAL 1195
Query: 557 -CVDNITSSAEPCEIPKPPQPVAAPS 631
+N A+ + PPQP PS
Sbjct: 1196 KSYENFLPEAKVTTVVTPPQPDVTPS 1221
>sp|P08569|MSP1_PLAFM Merozoite surface protein 1 precursor (Merozoite surface antigens)
(PMMSA) (P190)
Length = 1701
Score = 34.7 bits (78), Expect = 0.35
Identities = 42/146 (28%), Positives = 61/146 (41%), Gaps = 27/146 (18%)
Frame = +2
Query: 275 NKRKSNRNKSVMSTLS----LMKHSKGACKNQQGDSE-LQTLSCNSLRLWSSDKAKEKSV 439
NK+K +TL L+KH KG K G+S L+TLS S++ + EK
Sbjct: 1095 NKKKEAEIAETENTLENTKILLKHYKGLVKYYNGESSPLKTLSEVSIQTEDNYANLEKFR 1154
Query: 440 VLKRLLNEWNE------VQLTMASSTDYKLILE-----------GTIPSGNPNAKI---- 556
L ++ + N+ +L+ SS + LI E G PS N N K+
Sbjct: 1155 ALSKIDGKLNDNLHLGKKKLSFLSSGLHHLITELKEVIKNKNYTGNSPSEN-NKKVNEAL 1213
Query: 557 -CVDNITSSAEPCEIPKPPQPVAAPS 631
+N A+ + PPQP PS
Sbjct: 1214 KSYENFLPEAKVTTVVTPPQPDVTPS 1239
>sp|P13819|MSP1_PLAFF Merozoite surface protein 1 precursor (Merozoite surface antigens)
(PMMSA)
Length = 1701
Score = 34.7 bits (78), Expect = 0.35
Identities = 42/146 (28%), Positives = 61/146 (41%), Gaps = 27/146 (18%)
Frame = +2
Query: 275 NKRKSNRNKSVMSTLS----LMKHSKGACKNQQGDSE-LQTLSCNSLRLWSSDKAKEKSV 439
NK+K +TL L+KH KG K G+S L+TLS S++ + EK
Sbjct: 1095 NKKKEAEIAETENTLENTKILLKHYKGLVKYYNGESSPLKTLSEVSIQTEDNYANLEKFR 1154
Query: 440 VLKRLLNEWNE------VQLTMASSTDYKLILE-----------GTIPSGNPNAKI---- 556
L ++ + N+ +L+ SS + LI E G PS N N K+
Sbjct: 1155 ALSKIDGKLNDNLHLGKKKLSFLSSGLHHLITELKEVIKNKNYTGNSPSEN-NKKVNEAL 1213
Query: 557 -CVDNITSSAEPCEIPKPPQPVAAPS 631
+N A+ + PPQP PS
Sbjct: 1214 KSYENFLPEAKVTTVVTPPQPDVTPS 1239
>sp|Q9DCF1|TMM25_MOUSE Transmembrane protein 25 precursor
Length = 365
Score = 32.3 bits (72), Expect = 1.7
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +2
Query: 644 VELFGMTFAGALALGLTIPIAVFVVCLFERKRR--RGMKNKRIIRHADELDLPEA*IRLP 817
V L G+ AG LALG + + V CL RK + +G + + +D +L +RLP
Sbjct: 233 VPLLGIVVAGGLALGTLVGFSTLVACLVCRKEKKTKGPSRRPSLISSDSNNLKLNNVRLP 292
>sp|Q62469|ITA2_MOUSE Integrin alpha-2 precursor (Platelet membrane glycoprotein Ia) (GPIa)
(Collagen receptor) (VLA-2 alpha chain) (CD49b)
Length = 1178
Score = 32.3 bits (72), Expect = 1.7
Identities = 33/142 (23%), Positives = 60/142 (42%), Gaps = 2/142 (1%)
Frame = +2
Query: 371 ELQTLSCNSLRLWSSDKAKEKSVVLKRLLNEWNEVQLTMASST--DYKLILEGTIPSGNP 544
+ +T SC+++ W D + + WN T A+ST +L I + NP
Sbjct: 1046 DCRTTSCSNITCWLKDLHMKAEYFINVTTRVWNR---TFAASTFQTVQLTAAAEIDTHNP 1102
Query: 545 NAKICVDNITSSAEPCEIPKPPQPVAAPSFTSWVELFGMTFAGALALGLTIPIAVFVVCL 724
+ +N + P I KP + P+ + G AG L L L + ++ +
Sbjct: 1103 QLFVIEENAVTI--PLMIMKPTEKAEVPTGV----IIGSIIAGILLL-LAMTAGLWKLGF 1155
Query: 725 FERKRRRGMKNKRIIRHADELD 790
F+R + K++ ++ DE+D
Sbjct: 1156 FKR------QYKKMGQNPDEMD 1171
>sp|Q09388|ACM2_CAEEL Probable muscarinic acetylcholine receptor gar-2 (G-protein linked
acetylcholine receptor 2)
Length = 627
Score = 31.6 bits (70), Expect = 3.0
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = +2
Query: 209 KNQDSTKTTKVISCMRFVYRIDNKRKSNRNKSVMSTLSLMKHSK 340
K+ K ++I+ MR + I +KRK+N+NKSV+S L+ + K
Sbjct: 491 KSTAGGKVRRLITQMRS-HSIRSKRKANKNKSVLSALNFFQRKK 533
>sp|Q25188|OAR_HELVI Octopamine receptor
Length = 477
Score = 31.6 bits (70), Expect = 3.0
Identities = 42/181 (23%), Positives = 65/181 (35%), Gaps = 12/181 (6%)
Frame = +2
Query: 215 QDSTKTTKVISCMRFVYRIDNKRKSNRNKSVMSTLSLMKHSKGACKNQQGDSELQTLSCN 394
+D K TK+ + + +NK + SV S + +H + + +T
Sbjct: 244 RDRAKATKISTISSGQNKYNNKDDHHDQDSVSSEANHNEHQGVTRLVSDNEKKKRTRKLT 303
Query: 395 -----SLRLWSSDKAKEKSVVLKRLLNEWNEVQLTMASSTDYKLILEGTIPSGNPNAKIC 559
+ WS D + +++ L NE S TD LE S N+K
Sbjct: 304 PKKKPKRKYWSKDDKSQNKLIIPILSNE--------NSVTDMGENLENRNTSSESNSKET 355
Query: 560 -VDNITSSAEPCEI------PKPPQPVAAPSFTSWVELFGMTFAGALALGLTIPIAVFVV 718
D++ E + PKP Q A F + +T A L I + VFVV
Sbjct: 356 HEDDLIEVNETVPVKTHHKKPKPNQQSAVYQFIEEKQRISLTRERRAARTLGIIMGVFVV 415
Query: 719 C 721
C
Sbjct: 416 C 416
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,789,447
Number of Sequences: 369166
Number of extensions: 1797228
Number of successful extensions: 5554
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5552
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)