Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_K03
(515 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 119 3e-27
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 117 2e-26
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 115 8e-26
sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (... 114 1e-25
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 114 1e-25
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 113 2e-25
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 113 2e-25
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 113 2e-25
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 112 5e-25
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 112 7e-25
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 119 bits (299), Expect = 3e-27
Identities = 70/149 (46%), Positives = 92/149 (61%), Gaps = 4/149 (2%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDD-DGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYY 178
EGL++LH +I+HRDIK DN+L++ G +K++D G R AG TF GT Y
Sbjct: 789 EGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSK---RLAGINPCTETFTGTLQY 845
Query: 179 MAPEIIIG--EGYSFGADIWALGVTVIEMATGKRPYQEM-VPQAVMYKIGNTKTIPYQLP 349
MAPEII GY ADIW+LG T+IEMATGK P+ E+ PQA M+K+G K P ++P
Sbjct: 846 MAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHP-EIP 904
Query: 350 VSSPIQMKQFIDKTLIPEPSERPSATDLI 436
S + K FI K P+P +R A DL+
Sbjct: 905 ESMSAEAKAFILKCFEPDPDKRACANDLL 933
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 117 bits (293), Expect = 2e-26
Identities = 67/145 (46%), Positives = 89/145 (61%), Gaps = 1/145 (0%)
Frame = +2
Query: 5 GLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYMA 184
GL++LH I+HRDIK NIL+D+ G ++L D G + A A + GT Y+MA
Sbjct: 183 GLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELAT-VNGAKSMKGTPYWMA 241
Query: 185 PEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQ-AVMYKIGNTKTIPYQLPVSSP 361
PE+I+ G+SF ADIW++G TVIEMATGK P+ E Q A + IG TK P +P
Sbjct: 242 PEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHP-PIPEDLS 300
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
+ K F+ K L EPS R SAT+L+
Sbjct: 301 PEAKDFLMKCLHKEPSLRLSATELL 325
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 115 bits (287), Expect = 8e-26
Identities = 62/145 (42%), Positives = 91/145 (62%), Gaps = 1/145 (0%)
Frame = +2
Query: 5 GLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYMA 184
GL++LH I+HRDIK NIL+D+ G +KL D G + A + A + GT Y+MA
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELAT-ISGAKSMKGTPYWMA 241
Query: 185 PEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQ-AVMYKIGNTKTIPYQLPVSSP 361
PE+I+ G+SF ADIW++G TVIEM TGK P+ + + A ++ IG TK+ P +P +
Sbjct: 242 PEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHP-PIPDNIS 300
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
F+ K L EP+ RP+A++L+
Sbjct: 301 SDANDFLLKCLQQEPNLRPTASELL 325
>sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (Protein kinase 75490 D)
Length = 655
Score = 114 bits (286), Expect = 1e-25
Identities = 63/147 (42%), Positives = 83/147 (56%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYM 181
+GL+FLH KKIIHRDIK DNILL+ G VK+TD G + L ++ T GT Y+M
Sbjct: 493 QGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTEKR---SKRATMVGTPYWM 549
Query: 182 APEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSP 361
APEI+ +GY D+W+LG+ +IEM G+ PY P +Y I N + + P S
Sbjct: 550 APEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPESVS 609
Query: 362 IQMKQFIDKTLIPEPSERPSATDLINF 442
Q KQF+D L R S L+ F
Sbjct: 610 KQTKQFLDACLQVNVESRASVRKLLTF 636
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 114 bits (286), Expect = 1e-25
Identities = 63/145 (43%), Positives = 91/145 (62%), Gaps = 1/145 (0%)
Frame = +2
Query: 5 GLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYMA 184
GL++LH I+HRDIK NIL+D+ G +KL D G + A T A + GT Y+MA
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELAT-MTGAKSMKGTPYWMA 242
Query: 185 PEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQ-AVMYKIGNTKTIPYQLPVSSP 361
PE+I+ G+SF ADIW++G TVIEM TGK P+ + + A ++ IG TK+ P +P +
Sbjct: 243 PEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHP-PIPDTLS 301
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
K F+ K L P+ RP+A++L+
Sbjct: 302 SDAKDFLLKCLQEVPNLRPTASELL 326
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 113 bits (283), Expect = 2e-25
Identities = 62/145 (42%), Positives = 88/145 (60%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYM 181
+GL +LH +K IHRDIK N+LL + G VKL D G+ L T + NTF GT ++M
Sbjct: 142 KGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQ---IKRNTFVGTPFWM 198
Query: 182 APEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSP 361
APE+I Y ADIW+LG+T IE+A G+ P+ E+ P V++ I K P L +
Sbjct: 199 APEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLI--PKNNPPTLEGNYS 256
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
+K+F++ L EPS RP+A +L+
Sbjct: 257 KPLKEFVEACLNKEPSFRPTAKELL 281
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 113 bits (283), Expect = 2e-25
Identities = 58/145 (40%), Positives = 84/145 (57%)
Frame = +2
Query: 5 GLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYMA 184
GL+FLH K ++HRDIK DNILL +G++KLTD G + + T GT Y+MA
Sbjct: 726 GLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINEL---NLKRTTMVGTPYWMA 782
Query: 185 PEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSPI 364
PE++ + Y DIW+LG+ +IEM G+ PY P +Y I T + P +
Sbjct: 783 PEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSS 842
Query: 365 QMKQFIDKTLIPEPSERPSATDLIN 439
+K+F+D L EP +R SAT+L++
Sbjct: 843 SLKKFLDWCLCVEPEDRASATELLH 867
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 113 bits (283), Expect = 2e-25
Identities = 62/145 (42%), Positives = 88/145 (60%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYM 181
+GL +LH +K IHRDIK N+LL + G VKL D G+ L T + NTF GT ++M
Sbjct: 130 KGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQ---IKRNTFVGTPFWM 186
Query: 182 APEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSP 361
APE+I Y ADIW+LG+T IE+A G+ P+ E+ P V++ I K P L +
Sbjct: 187 APEVIKQSAYDSKADIWSLGITAIELAKGEPPHSELHPMKVLFLI--PKNNPPTLEGNYS 244
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
+K+F++ L EPS RP+A +L+
Sbjct: 245 KPLKEFVEACLNKEPSFRPTAKELL 269
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 112 bits (280), Expect = 5e-25
Identities = 63/145 (43%), Positives = 86/145 (59%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYM 181
+GL +LH +K IHRDIK N+LL + G+VKL D G+ L T + NTF GT ++M
Sbjct: 130 KGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQ---IKRNTFVGTPFWM 186
Query: 182 APEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSP 361
APE+I Y ADIW+LG+T IE+A G+ P +M P V++ I K P L
Sbjct: 187 APEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLVGDFT 244
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
K+FID L +PS RP+A +L+
Sbjct: 245 KSFKEFIDACLNKDPSFRPTAKELL 269
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 112 bits (279), Expect = 7e-25
Identities = 63/145 (43%), Positives = 86/145 (59%)
Frame = +2
Query: 2 EGLQFLHRKKIIHRDIKPDNILLDDDGNVKLTDLGLGLSLCRTAGHTTQANTFCGTTYYM 181
+GL +LH +K IHRDIK N+LL + G+VKL D G+ L T + NTF GT ++M
Sbjct: 130 KGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQ---IKRNTFVGTPFWM 186
Query: 182 APEIIIGEGYSFGADIWALGVTVIEMATGKRPYQEMVPQAVMYKIGNTKTIPYQLPVSSP 361
APE+I Y ADIW+LG+T IE+A G+ P +M P V++ I K P L
Sbjct: 187 APEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLIGDFT 244
Query: 362 IQMKQFIDKTLIPEPSERPSATDLI 436
K+FID L +PS RP+A +L+
Sbjct: 245 KSFKEFIDACLNKDPSFRPTAKELL 269
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,868,665
Number of Sequences: 369166
Number of extensions: 1355986
Number of successful extensions: 7245
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5501
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)