Planarian EST Database


Dr_sW_012_J22

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_012_J22
         (648 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6X632|GPR75_MOUSE  Probable G-protein coupled receptor 75      35   0.22 
sp|Q8D2X6|IF2_WIGBR  Translation initiation factor IF-2            35   0.22 
sp|O95800|GPR75_HUMAN  Probable G-protein coupled receptor 75      33   0.49 
sp|O35568|FBLN3_RAT  EGF-containing fibulin-like extracellul...    33   0.64 
sp|Q58318|Y908_METJA  Hypothetical protein MJ0908                  32   1.4  
sp|Q8BPB5|FBLN3_MOUSE  EGF-containing fibulin-like extracell...    31   2.4  
sp|Q8XJ27|SYH_CLOPE  Histidyl-tRNA synthetase (Histidine--tR...    31   2.4  
sp|Q6CT96|GNT1B_KLULA  Glucose N-acetyltransferase 1-B (N-ac...    31   3.2  
sp|P47533|P69_MYCGE  Transport system permease protein p69         31   3.2  
sp|Q86XX4|FRAS1_HUMAN  Extracellular matrix protein FRAS1 pr...    30   4.1  
>sp|Q6X632|GPR75_MOUSE Probable G-protein coupled receptor 75
          Length = 540

 Score = 34.7 bits (78), Expect = 0.22
 Identities = 20/43 (46%), Positives = 27/43 (62%)
 Frame = -1

Query: 243 FFDASFR*SRFTLSTKFFIMNITGFIELFIISVTIPIKDLVLF 115
           FFD SFR  +F  +  F I+N++ F +LFI  VT P+   VLF
Sbjct: 67  FFDPSFR--KFRTNFDFMILNLS-FCDLFICGVTAPMFTFVLF 106
>sp|Q8D2X6|IF2_WIGBR Translation initiation factor IF-2
          Length = 841

 Score = 34.7 bits (78), Expect = 0.22
 Identities = 34/110 (30%), Positives = 53/110 (48%), Gaps = 17/110 (15%)
 Frame = +1

Query: 46  INIKRKVWLTCLKFL--------NEIDAECDKKYEILDRNCDGNYKQLYKTCN---IHYE 192
           I +++K +L C   L        N ID    KK  I D N    Y +L K  N   I++E
Sbjct: 84  IEVRKKKYLKCDSSLLFKDKIVKNNIDKTFCKK-NIKDLNKTKKYVELSKNINEREINFE 142

Query: 193 K------LSGKCKARLSKTCIKKYGKLNKDCIDAYDKIDVSCDKEYSKLD 324
           K      +  K K ++SK  IKK  K+NK+ ++ Y K +   + + +KL+
Sbjct: 143 KKLIKEPILDKSKLKISKNLIKKNVKINKN-LNIYKKNEFKQNLKKNKLN 191
>sp|O95800|GPR75_HUMAN Probable G-protein coupled receptor 75
          Length = 540

 Score = 33.5 bits (75), Expect = 0.49
 Identities = 19/43 (44%), Positives = 27/43 (62%)
 Frame = -1

Query: 243 FFDASFR*SRFTLSTKFFIMNITGFIELFIISVTIPIKDLVLF 115
           FFD +FR  +F  +  F I+N++ F +LFI  VT P+   VLF
Sbjct: 67  FFDPAFR--KFRTNFDFMILNLS-FCDLFICGVTAPMFTFVLF 106
>sp|O35568|FBLN3_RAT EGF-containing fibulin-like extracellular matrix protein 1
           precursor (Fibulin-3) (FIBL-3) (T16 protein)
          Length = 493

 Score = 33.1 bits (74), Expect = 0.64
 Identities = 20/66 (30%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
 Frame = +1

Query: 142 CDGNYKQL-YKTCNIHYEKLSGKCKARLSKTCIKKYGKLNKDCIDAYDKIDVSC-DKEYS 315
           C   Y+Q  +  C    E  SG    RL + CI   G     C+  Y K    C D +  
Sbjct: 159 CAAGYEQSEHNVCQDIDECTSGTHNCRLDQVCINLRGSFTCHCLPGYQKRGEQCVDIDEC 218

Query: 316 KLDPYC 333
            + PYC
Sbjct: 219 SVPPYC 224
>sp|Q58318|Y908_METJA Hypothetical protein MJ0908
          Length = 1232

 Score = 32.0 bits (71), Expect = 1.4
 Identities = 24/83 (28%), Positives = 43/83 (51%), Gaps = 6/83 (7%)
 Frame = +1

Query: 148 GNYKQLYKTCNIHYEKLSGKCKARLSKTCIKKYG--KLNKDCIDA----YDKIDVSCDKE 309
           G+  +L K+ + +YE  + + K  L K  +KK    KL++D +D      +  D + +K 
Sbjct: 615 GDLVELEKSIDDYYETENKEKKEFLKKEILKKIKELKLDEDLLDGKVIDEEINDENFEKL 674

Query: 310 YSKLDPYCDTLYNQQAKCGVETM 378
            +K+  Y +TL N+Q   G+  M
Sbjct: 675 LNKIHDYLETLKNRQINDGLHIM 697
>sp|Q8BPB5|FBLN3_MOUSE EGF-containing fibulin-like extracellular matrix protein 1
           precursor (Fibulin-3) (FIBL-3)
          Length = 493

 Score = 31.2 bits (69), Expect = 2.4
 Identities = 19/66 (28%), Positives = 25/66 (37%), Gaps = 2/66 (3%)
 Frame = +1

Query: 142 CDGNYKQL-YKTCNIHYEKLSGKCKARLSKTCIKKYGKLNKDCIDAYDKIDVSC-DKEYS 315
           C   Y+Q  +  C    E  SG    R  + CI   G     C+  Y K    C D +  
Sbjct: 159 CAAGYEQSEHNVCQDIDECTSGTHNCRTDQVCINLRGSFTCQCLPGYQKRGEQCVDIDEC 218

Query: 316 KLDPYC 333
            + PYC
Sbjct: 219 TVPPYC 224
>sp|Q8XJ27|SYH_CLOPE Histidyl-tRNA synthetase (Histidine--tRNA ligase) (HisRS)
          Length = 415

 Score = 31.2 bits (69), Expect = 2.4
 Identities = 23/83 (27%), Positives = 35/83 (42%), Gaps = 4/83 (4%)
 Frame = +1

Query: 106 ECDKKY-EILDRNCDGNYKQLYKTCNIHYEKLSGK---CKARLSKTCIKKYGKLNKDCID 273
           +C  K+ E L +    NY  L +TC   +EK   +   CK +  K  +K+   +      
Sbjct: 171 KCRAKFNEALKQYLKENYDNLCETCKTRFEKNPMRIIDCKEKRCKEIVKEAPSI------ 224

Query: 274 AYDKIDVSCDKEYSKLDPYCDTL 342
             D I   C   +SKL  Y D +
Sbjct: 225 -LDYICEECSDHFSKLKAYLDVM 246
>sp|Q6CT96|GNT1B_KLULA Glucose N-acetyltransferase 1-B (N-acetylglucosaminyltransferase B)
          Length = 453

 Score = 30.8 bits (68), Expect = 3.2
 Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 10/63 (15%)
 Frame = +1

Query: 13  MGVAKTKKEELINIKR----------KVWLTCLKFLNEIDAECDKKYEILDRNCDGNYKQ 162
           +G  + +K++LI   +          K W     + N  D +C KKY I D NC   +K 
Sbjct: 384 LGYERKEKDDLIQDAKFIHFSDYPLSKPWF----YSNADDIQCSKKYSISDENCQ-LWKS 438

Query: 163 LYK 171
           LYK
Sbjct: 439 LYK 441
>sp|P47533|P69_MYCGE Transport system permease protein p69
          Length = 543

 Score = 30.8 bits (68), Expect = 3.2
 Identities = 22/73 (30%), Positives = 31/73 (42%), Gaps = 2/73 (2%)
 Frame = -1

Query: 216 RFTLSTKFFIM--NITGFIELFIISVTIPIKDLVLFVTLGINLI*EF*TSQPHLSLNIYQ 43
           RF   T FF+   +  GFI   + S     K    ++ L I LI         + L  + 
Sbjct: 72  RFLAQTAFFVTGGSFLGFIFAILFSYWTAFKIQPFYIALPIRLI--------TIVLRAFP 123

Query: 42  FLFFGFCYSHTFN 4
            L FGF +S+ FN
Sbjct: 124 VLLFGFLFSNLFN 136
>sp|Q86XX4|FRAS1_HUMAN Extracellular matrix protein FRAS1 precursor
          Length = 4007

 Score = 30.4 bits (67), Expect = 4.1
 Identities = 23/97 (23%), Positives = 36/97 (37%), Gaps = 8/97 (8%)
 Frame = +1

Query: 106 ECDKKYEILDRNCDGNYKQLYKTCNIHYEKLSGKCKARLSKTCIKKYGKLNKDCIDAYDK 285
           +C   + +LD  C          C   Y    G C      TC + +G L  DCI  Y  
Sbjct: 731 DCGPSHVLLDGQC-------LSQCPDGYFHQEGSC-TECHPTCRQCHGPLESDCISCYPH 782

Query: 286 IDV-------SC-DKEYSKLDPYCDTLYNQQAKCGVE 372
           I +       SC ++++  L  YC   ++    C  +
Sbjct: 783 ISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAAD 819
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,101,434
Number of Sequences: 369166
Number of extensions: 1214014
Number of successful extensions: 3712
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3707
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5316264630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)