Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_I18
(748 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P34897|GLYM_HUMAN Serine hydroxymethyltransferase, mitoc... 321 1e-87
sp|P34896|GLYC_HUMAN Serine hydroxymethyltransferase, cytos... 321 1e-87
sp|P35623|GLYC_SHEEP Serine hydroxymethyltransferase, cytos... 318 1e-86
sp|P14519|GLYM_RABIT Serine hydroxymethyltransferase, mitoc... 316 4e-86
sp|P50431|GLYC_MOUSE Serine hydroxymethyltransferase, cytos... 316 5e-86
sp|P07511|GLYC_RABIT Serine hydroxymethyltransferase, cytos... 315 6e-86
sp|Q9SZJ5|GLYM_ARATH Serine hydroxymethyltransferase, mitoc... 304 1e-82
sp|P34899|GLYM_PEA Serine hydroxymethyltransferase, mitocho... 303 2e-82
sp|P50433|GLYM_SOLTU Serine hydroxymethyltransferase, mitoc... 301 2e-81
sp|P50432|GLYC_CAEEL Serine hydroxymethyltransferase (Serin... 301 2e-81
>sp|P34897|GLYM_HUMAN Serine hydroxymethyltransferase, mitochondrial precursor (Serine
methylase) (Glycine hydroxymethyltransferase) (SHMT)
Length = 504
Score = 321 bits (823), Expect = 1e-87
Identities = 159/231 (68%), Positives = 190/231 (82%)
Frame = +1
Query: 55 TSQKLLRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGA 234
T Q+ L DSDPE++EL+ +EK RQ GL+LIASENF S L+ALGSCL NKY+EGY G
Sbjct: 44 TGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGK 103
Query: 235 RYYGGTEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMG 414
RYYGG E+V++IE R L+ + LDP+ WGVNVQPYSGSPAN AVYTA+LQPHDRIMG
Sbjct: 104 RYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAVYTALLQPHDRIMG 163
Query: 415 LDLPDGGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIA 594
LDLPDGGHLTHG+M+ KRISATSI+FESMPYK++ +GLIDY+QL + LF+P+LIIA
Sbjct: 164 LDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPKTGLIDYNQLALTARLFRPRLIIA 223
Query: 595 GISAYSRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
G SAY+RL+DYAR R++ D V A L ADMAHISGLVAA VIPSPF++ADIV
Sbjct: 224 GTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIV 274
>sp|P34896|GLYC_HUMAN Serine hydroxymethyltransferase, cytosolic (Serine methylase)
(Glycine hydroxymethyltransferase) (SHMT)
Length = 483
Score = 321 bits (823), Expect = 1e-87
Identities = 156/226 (69%), Positives = 185/226 (81%)
Frame = +1
Query: 70 LRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGG 249
L+DSD E++ +I KE RQR GL+LIASENF+S VL+ALGSCL NKY+EGY G RYYGG
Sbjct: 26 LKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGG 85
Query: 250 TEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPD 429
TE ++++ET R L Y+LDP WGVNVQPYSGSPANFAVYTA+++PH RIMGLDLPD
Sbjct: 86 TEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPD 145
Query: 430 GGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAY 609
GGHLTHGFMT K+ISATSI+FESMPYKV+ +G I+YDQLE++ LF PKLIIAG S Y
Sbjct: 146 GGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCY 205
Query: 610 SRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
SR L+YAR R+I+D GA L ADMAHISGLVAA V+PSPFE+ +V
Sbjct: 206 SRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVV 251
>sp|P35623|GLYC_SHEEP Serine hydroxymethyltransferase, cytosolic (Serine methylase)
(Glycine hydroxymethyltransferase) (SHMT)
Length = 484
Score = 318 bits (815), Expect = 1e-86
Identities = 154/226 (68%), Positives = 185/226 (81%)
Frame = +1
Query: 70 LRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGG 249
L+D+D E++ +I KE RQR GL+LIASENF+S VL+ALGSCL NKY+EGY G RYYGG
Sbjct: 26 LKDNDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGG 85
Query: 250 TEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPD 429
TE ++++E R L +Y LDP WGVNVQPYSGSPANFAVYTA+++PH RIMGLDLPD
Sbjct: 86 TEFIDELEVLCQKRALQVYGLDPECWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPD 145
Query: 430 GGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAY 609
GGHLTHGFMT K+ISATSI+FESMPYKV+ +G I+YDQLE++ LF P+LIIAG S Y
Sbjct: 146 GGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPRLIIAGTSCY 205
Query: 610 SRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
SR LDYAR R+I+D+ GA L ADMAHISGLVAA V+PSPFE+ +V
Sbjct: 206 SRNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVV 251
>sp|P14519|GLYM_RABIT Serine hydroxymethyltransferase, mitochondrial precursor (Serine
methylase) (Glycine hydroxymethyltransferase) (SHMT)
Length = 504
Score = 316 bits (810), Expect = 4e-86
Identities = 154/231 (66%), Positives = 188/231 (81%)
Frame = +1
Query: 55 TSQKLLRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGA 234
T Q+ L D+DPE++EL+ +EK RQ GL+LIASENF L+ALGSCL NKY+EGY G
Sbjct: 44 TGQESLSDTDPEMWELLQREKDRQCRGLELIASENFCIRAALEALGSCLNNKYSEGYPGK 103
Query: 235 RYYGGTEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMG 414
RYYGG E+V++IE R L+ + LDP+ WGVNVQPYSGSPAN A YTA+LQPHDRIMG
Sbjct: 104 RYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAAYTALLQPHDRIMG 163
Query: 415 LDLPDGGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIA 594
LDLPDGGHLTHG+M+ KR+SATSI+FESMPYK++ +GLIDY+QL + LF+P+LIIA
Sbjct: 164 LDLPDGGHLTHGYMSDVKRVSATSIFFESMPYKLNPQTGLIDYEQLALTARLFRPRLIIA 223
Query: 595 GISAYSRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
G SAY+RL+DYAR R++ D V A L ADMAHISGLVAA VIPSPF++AD+V
Sbjct: 224 GTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADVV 274
>sp|P50431|GLYC_MOUSE Serine hydroxymethyltransferase, cytosolic (Serine methylase)
(Glycine hydroxymethyltransferase) (SHMT)
Length = 478
Score = 316 bits (809), Expect = 5e-86
Identities = 155/226 (68%), Positives = 182/226 (80%)
Frame = +1
Query: 70 LRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGG 249
L+DSD E++ +I KE RQR GL+LIASENF+S VL+ALGS L NKY+EGY G RYYGG
Sbjct: 20 LKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSSLNNKYSEGYPGQRYYGG 79
Query: 250 TEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPD 429
TE ++++E R L Y LDP WGVNVQPYSGSPANFAVYTA+++PH RIMGLDLPD
Sbjct: 80 TEFIDELEMLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPD 139
Query: 430 GGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAY 609
GGHLTHGFMT K+ISATSI+FESMPYKV +G I+YDQLE++ LF PKLIIAG S Y
Sbjct: 140 GGHLTHGFMTDKKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCY 199
Query: 610 SRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
SR LDYAR R+I+D+ GA L ADMAHISGLVAA V+PSPFE+ +V
Sbjct: 200 SRNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVV 245
>sp|P07511|GLYC_RABIT Serine hydroxymethyltransferase, cytosolic (Serine methylase)
(Glycine hydroxymethyltransferase) (SHMT)
Length = 484
Score = 315 bits (808), Expect = 6e-86
Identities = 154/226 (68%), Positives = 182/226 (80%)
Frame = +1
Query: 70 LRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGG 249
L+DSD E++++I KE RQR GL+LIASENF+S VL+ALGSCL NKY+EGY G RYYGG
Sbjct: 26 LKDSDAEVYDIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGG 85
Query: 250 TEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPD 429
TE ++++ET R L Y LDP WGVNVQPYSGSPANFAVYTA+++PH RIMGLDLPD
Sbjct: 86 TEHIDELETLCQKRALQAYGLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPD 145
Query: 430 GGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAY 609
GGHLTHGFMT K+ISATSI+FESM YKV+ +G IDYD+LE++ LF PKLIIAG S Y
Sbjct: 146 GGHLTHGFMTDKKKISATSIFFESMAYKVNPDTGYIDYDRLEENARLFHPKLIIAGTSCY 205
Query: 610 SRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
SR LDY R R+I+D GA L ADMAHISGLV A V+PSPFE+ +V
Sbjct: 206 SRNLDYGRLRKIADENGAYLMADMAHISGLVVAGVVPSPFEHCHVV 251
>sp|Q9SZJ5|GLYM_ARATH Serine hydroxymethyltransferase, mitochondrial precursor (Serine
methylase) (Glycine hydroxymethyltransferase) (SHMT)
Length = 517
Score = 304 bits (779), Expect = 1e-82
Identities = 146/226 (64%), Positives = 181/226 (80%)
Frame = +1
Query: 70 LRDSDPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGG 249
L + DPEI ++I EK RQ GL+LI SENF+SV V+QA+GS +TNKY+EGY GARYYGG
Sbjct: 55 LEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGG 114
Query: 250 TEIVNKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPD 429
E ++ ET R L+ +RLDP WGVNVQP SGSPANF VYTA+L+PH+RIM LDLP
Sbjct: 115 NEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPH 174
Query: 430 GGHLTHGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAY 609
GGHL+HG+ T K+ISA SI+FE+MPY++D +G IDYDQ+EKS LF+PKLI+AG SAY
Sbjct: 175 GGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAY 234
Query: 610 SRLLDYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
+RL DYAR R++ + A++ ADMAHISGLVAANVIPSPF+YAD+V
Sbjct: 235 ARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVV 280
>sp|P34899|GLYM_PEA Serine hydroxymethyltransferase, mitochondrial precursor (Serine
methylase) (Glycine hydroxymethyltransferase) (SHMT)
Length = 518
Score = 303 bits (777), Expect = 2e-82
Identities = 146/222 (65%), Positives = 179/222 (80%)
Frame = +1
Query: 82 DPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGGTEIV 261
DPEI ++I EK RQ GL+LI SENF+S+ V+QA+GS +TNKY+EGY GARYYGG E +
Sbjct: 60 DPEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 119
Query: 262 NKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPDGGHL 441
+ ET R L+ +RLDP+ WGVNVQP SGSP+NF VYTA+L+PHDRIM LDLP GGHL
Sbjct: 120 DMAETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHL 179
Query: 442 THGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAYSRLL 621
+HG+ T K+ISA SI+FE+MPY++D +G IDYDQLEKS LF+PKLI+AG SAY+RL
Sbjct: 180 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLY 239
Query: 622 DYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
DYAR R++ D A+L ADMAHISGLVAA VIPSPF+YAD+V
Sbjct: 240 DYARIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVV 281
>sp|P50433|GLYM_SOLTU Serine hydroxymethyltransferase, mitochondrial precursor (Serine
methylase) (Glycine hydroxymethyltransferase) (SHMT)
Length = 518
Score = 301 bits (770), Expect = 2e-81
Identities = 146/222 (65%), Positives = 178/222 (80%)
Frame = +1
Query: 82 DPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGGTEIV 261
DPEI ++I EK RQ GL+LI SENF+SV V+QA+GS +TNKY+EGY GARYYGG E +
Sbjct: 60 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 119
Query: 262 NKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPDGGHL 441
+ ET R L+ +RLDP+ WGVNVQP SGSPANF VYTA+L+PH+RIM LDLP GGHL
Sbjct: 120 DMAETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHL 179
Query: 442 THGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAYSRLL 621
+HG+ T K+ISA SI+FE+MPY++D +G IDYDQLEKS LF+PKLI+AG SAY+RL
Sbjct: 180 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLY 239
Query: 622 DYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
DY R R++ + AIL ADMAHISGLVAA VIPSPF+YAD+V
Sbjct: 240 DYDRIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVV 281
>sp|P50432|GLYC_CAEEL Serine hydroxymethyltransferase (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) (Glycosylation related
1)
Length = 507
Score = 301 bits (770), Expect = 2e-81
Identities = 141/222 (63%), Positives = 182/222 (81%)
Frame = +1
Query: 82 DPEIFELICKEKQRQRTGLQLIASENFSSVGVLQALGSCLTNKYAEGYSGARYYGGTEIV 261
DPE+F+++ EK+RQR GL+LIASENF+S V+ ALGS + NKY+EGY GARYYGG E +
Sbjct: 56 DPEVFDIMKNEKKRQRRGLELIASENFTSKAVMDALGSAMCNKYSEGYPGARYYGGNEFI 115
Query: 262 NKIETTACSRLLDLYRLDPSVWGVNVQPYSGSPANFAVYTAVLQPHDRIMGLDLPDGGHL 441
+++E R L+++ LDP+ WGVNVQP SGSPANFAVYTA++ + RIMGLDLPDGGHL
Sbjct: 116 DQMELLCQKRALEVFGLDPAKWGVNVQPLSGSPANFAVYTAIVGSNGRIMGLDLPDGGHL 175
Query: 442 THGFMTLDKRISATSIYFESMPYKVDAMSGLIDYDQLEKSTILFKPKLIIAGISAYSRLL 621
THGF T +++SATS +F+S+PYKVD +GLIDYD+LE++ +LF+PK IIAG+S Y+R L
Sbjct: 176 THGFFTPARKVSATSEFFQSLPYKVDPTTGLIDYDKLEQNAMLFRPKAIIAGVSCYARHL 235
Query: 622 DYARFRQISDNVGAILHADMAHISGLVAANVIPSPFEYADIV 747
DY RFR+I+ GA L +DMAHISGLVAA +IPSPFEY+D+V
Sbjct: 236 DYERFRKIATKAGAYLMSDMAHISGLVAAGLIPSPFEYSDVV 277
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,128,462
Number of Sequences: 369166
Number of extensions: 1745124
Number of successful extensions: 4710
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4329
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)