Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_I10
(438 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P37167|ACTP_ACACA Actophorin 64 2e-10
sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) ... 59 6e-09
sp|P60982|DEST_PIG Destrin (Actin-depolymerizing factor) (A... 56 4e-08
sp|P23528|COF1_HUMAN Cofilin-1 (Cofilin, non-muscle isoform... 55 5e-08
sp|P45592|COF1_RAT Cofilin-1 (Cofilin, non-muscle isoform) 55 5e-08
sp|Q9R0P5|DEST_MOUSE Destrin (Actin-depolymerizing factor) ... 55 5e-08
sp|Q9Y281|COF2_HUMAN Cofilin-2 (Cofilin, muscle isoform) 55 7e-08
sp|P18760|COF1_MOUSE Cofilin-1 (Cofilin, non-muscle isoform) 55 7e-08
sp|P45591|COF2_MOUSE Cofilin-2 (Cofilin, muscle isoform) 55 7e-08
sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 55 7e-08
>sp|P37167|ACTP_ACACA Actophorin
Length = 138
Score = 63.5 bits (153), Expect = 2e-10
Identities = 32/117 (27%), Positives = 57/117 (48%), Gaps = 8/117 (6%)
Frame = +1
Query: 4 QYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE--------GTL 159
+YV + +N ++ G YE FK + E + YA++DYE +
Sbjct: 24 RYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDCR---YAIFDYEFQVDGGQRNKI 80
Query: 160 AFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEKTASKV 330
FILW P++ I S+M+ ++K I ++VG++ +++A EI E + E+ V
Sbjct: 81 TFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQATDAAEISEDAVSERAKKDV 137
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF)
Length = 165
Score = 58.5 bits (140), Expect = 6e-09
Identities = 38/116 (32%), Positives = 59/116 (50%), Gaps = 14/116 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 147
VI+ L+ +K II E GD+G +H +E ++ C YALYD
Sbjct: 36 VIFCLSPDKKCIIVEEGKEILVGDVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESK 95
Query: 148 EGTLAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 315
+ L F LW PE + S+MI A+SK I + G+K + +AN ++++ + I EK
Sbjct: 96 KEELMFFLWAPEQAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|P60982|DEST_PIG Destrin (Actin-depolymerizing factor) (ADF)
sp|P60981|DEST_HUMAN Destrin (Actin-depolymerizing factor) (ADF)
Length = 165
Score = 55.8 bits (133), Expect = 4e-08
Identities = 37/116 (31%), Positives = 58/116 (50%), Gaps = 14/116 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 147
VI+ L+ +K II E GD+G +H + ++ C YALYD
Sbjct: 36 VIFCLSADKKCIIVEEGKEILVGDVGVTITDPFKHFVGMLPEKDCRYALYDASFETKESR 95
Query: 148 EGTLAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 315
+ L F LW PE + S+MI A+SK I + G+K + +AN ++++ + I EK
Sbjct: 96 KEELMFFLWAPELAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|P23528|COF1_HUMAN Cofilin-1 (Cofilin, non-muscle isoform) (18 kDa phosphoprotein)
(p18)
Length = 166
Score = 55.5 bits (132), Expect = 5e-08
Identities = 38/117 (32%), Positives = 61/117 (52%), Gaps = 15/117 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT--- 156
V++ L+++K II E GD+G + ++ + C YALYD YE
Sbjct: 36 VLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESK 95
Query: 157 ---LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 315
L FI W PE+ + S+MI A+SK I ++ G+K +++AN +E+ D + EK
Sbjct: 96 KEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|P45592|COF1_RAT Cofilin-1 (Cofilin, non-muscle isoform)
Length = 166
Score = 55.5 bits (132), Expect = 5e-08
Identities = 38/117 (32%), Positives = 61/117 (52%), Gaps = 15/117 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT--- 156
V++ L+++K II E GD+G + ++ + C YALYD YE
Sbjct: 36 VLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESK 95
Query: 157 ---LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 315
L FI W PE+ + S+MI A+SK I ++ G+K +++AN +E+ D + EK
Sbjct: 96 KEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|Q9R0P5|DEST_MOUSE Destrin (Actin-depolymerizing factor) (ADF) (Sid 23)
Length = 165
Score = 55.5 bits (132), Expect = 5e-08
Identities = 36/116 (31%), Positives = 58/116 (50%), Gaps = 14/116 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 147
VI+ L+ +K I+ E GD+G +H + ++ C YALYD
Sbjct: 36 VIFCLSADKKCIVVEEGKEILVGDVGATITDPFKHFVGMLPEKDCRYALYDASFETKESR 95
Query: 148 EGTLAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 315
+ L F LW PE + S+MI A+SK I + G+K + +AN ++++ + I EK
Sbjct: 96 KEELMFFLWAPEQAPLKSKMIYASSKDAIKKKFPGIKHEYQANGPEDLNRTCIAEK 151
>sp|Q9Y281|COF2_HUMAN Cofilin-2 (Cofilin, muscle isoform)
Length = 166
Score = 55.1 bits (131), Expect = 7e-08
Identities = 41/122 (33%), Positives = 59/122 (48%), Gaps = 15/122 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLET-----GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT--- 156
V++ L+ +K +II E GD+G E ++ C YALYD YE
Sbjct: 36 VLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESK 95
Query: 157 ---LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI-DESVIKEKTAS 324
L FI W PE+ + S+MI A+SK I + G+K + + N D+I D S + EK
Sbjct: 96 KEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGG 155
Query: 325 KV 330
V
Sbjct: 156 NV 157
>sp|P18760|COF1_MOUSE Cofilin-1 (Cofilin, non-muscle isoform)
Length = 166
Score = 55.1 bits (131), Expect = 7e-08
Identities = 38/117 (32%), Positives = 60/117 (51%), Gaps = 15/117 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT--- 156
V++ L+++K II E GD+G + ++ + C YALYD YE
Sbjct: 36 VLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESK 95
Query: 157 ---LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 315
L FI W PE + S+MI A+SK I ++ G+K +++AN +E+ D + EK
Sbjct: 96 KEDLVFIFWAPENAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|P45591|COF2_MOUSE Cofilin-2 (Cofilin, muscle isoform)
Length = 166
Score = 55.1 bits (131), Expect = 7e-08
Identities = 41/122 (33%), Positives = 59/122 (48%), Gaps = 15/122 (12%)
Frame = +1
Query: 10 VIYGLNKEKNRIIKLET-----GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT--- 156
V++ L+ +K +II E GD+G E ++ C YALYD YE
Sbjct: 36 VLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESK 95
Query: 157 ---LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI-DESVIKEKTAS 324
L FI W PE+ + S+MI A+SK I + G+K + + N D+I D S + EK
Sbjct: 96 KEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGG 155
Query: 325 KV 330
V
Sbjct: 156 SV 157
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
Length = 140
Score = 55.1 bits (131), Expect = 7e-08
Identities = 28/112 (25%), Positives = 61/112 (54%), Gaps = 9/112 (8%)
Frame = +1
Query: 1 FQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGT-------- 156
++++++ ++++ +++ + G+ YE F + I E E YA+YDY+ T
Sbjct: 27 YRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE---DECRYAVYDYDFTTPENCQKS 83
Query: 157 -LAFILWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIK 309
+ FI W P+T ++ S+M+ A+SK + G++ +++A E+ +IK
Sbjct: 84 KIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSLDIIK 135
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,018,670
Number of Sequences: 369166
Number of extensions: 698063
Number of successful extensions: 1600
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1565
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1596
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2244666600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)