Planarian EST Database


Dr_sW_012_I08

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_012_I08
         (552 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9BW30|CG38_HUMAN  Protein CGI-38                               82   1e-15
sp|Q9CRB6|CG38_MOUSE  Protein CGI-38 homolog                       80   4e-15
sp|Q7TQD2|P25A_MOUSE  Tubulin polymerization-promoting prote...    76   6e-14
sp|O94811|P25A_HUMAN  Tubulin polymerization-promoting prote...    75   1e-13
sp|P59282|P25B_HUMAN  Protein p25-beta                             74   2e-13
sp|Q27957|P25A_BOVIN  Tubulin polymerization-promoting prote...    73   4e-13
sp|P91127|YBYK_CAEEL  Hypothetical protein C32E8.3 in chromo...    65   1e-10
sp|Q9VV43|Y4893_DROME  Hypothetical protein CG4893                 64   2e-10
sp|Q4L5U2|TRMD_STAHJ  tRNA (Guanine-N(1)-)-methyltransferase...    33   0.61 
sp|P16342|R1AB_CVMA5  Replicase polyprotein 1ab (pp1ab) (ORF...    32   1.4  
>sp|Q9BW30|CG38_HUMAN Protein CGI-38
          Length = 176

 Score = 81.6 bits (200), Expect = 1e-15
 Identities = 49/125 (39%), Positives = 67/125 (53%)
 Frame = +2

Query: 35  DIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHG 214
           DI F K  G + + ++Y +F + +E    + +         DA+ +L   +    PA  G
Sbjct: 56  DIVFSKVKGKSARVINYEEFKKALEELATKRFKGKSKEEAFDAICQL---VAGKEPANVG 112

Query: 215 TTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAG 394
            T      A  RLTD   YTG+HKERFD E+GKGKGI GR+ +  ++   GYV  YK AG
Sbjct: 113 VTKAKTGGAVDRLTDTSRYTGSHKERFD-ESGKGKGIAGRQDILDDS---GYVSAYKNAG 168

Query: 395 TYDKK 409
           TYD K
Sbjct: 169 TYDAK 173
>sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog
          Length = 176

 Score = 79.7 bits (195), Expect = 4e-15
 Identities = 49/125 (39%), Positives = 66/125 (52%)
 Frame = +2

Query: 35  DIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHG 214
           DI F K    + + ++Y +F + +E    + +         DA+ +L   I    PA  G
Sbjct: 56  DIVFSKVKAKSARVINYEEFKKALEELATKRFKGKSKEEAFDAICQL---IAGKEPANIG 112

Query: 215 TTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAG 394
            T      A  RLTD   YTG+HKERFD E+GKGKGI GR+ +  ++   GYV  YK AG
Sbjct: 113 VTKAKTGGAVDRLTDTSKYTGSHKERFD-ESGKGKGIAGRQDILDDS---GYVSAYKNAG 168

Query: 395 TYDKK 409
           TYD K
Sbjct: 169 TYDAK 173
>sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting protein (TPPP)
          Length = 218

 Score = 75.9 bits (185), Expect = 6e-14
 Identities = 48/125 (38%), Positives = 70/125 (56%)
 Frame = +2

Query: 35  DIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHG 214
           DI F K  G + + + +  F   +E    + +   ++ + E+AV+E+   I   +P   G
Sbjct: 96  DIVFSKIKGKSCRTITFEQFQEALEELAKKRF---KDKSSEEAVREVHRLIEGRAPVISG 152

Query: 215 TTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAG 394
            T   +    +RLTD   +TG+HKERFD ++GKGKG  GR  L  E+   GYV GYK AG
Sbjct: 153 VTKAVSSPTVSRLTDTSKFTGSHKERFD-QSGKGKGKAGRVDLVDES---GYVPGYKHAG 208

Query: 395 TYDKK 409
           TYD+K
Sbjct: 209 TYDQK 213
>sp|O94811|P25A_HUMAN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha) (p24) (p25)
          Length = 219

 Score = 74.7 bits (182), Expect = 1e-13
 Identities = 48/125 (38%), Positives = 69/125 (55%)
 Frame = +2

Query: 35  DIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHG 214
           DI F K  G + + + +  F   +E    + +   ++ + E+AV+E+   I   +P   G
Sbjct: 97  DIVFSKIKGKSCRTITFEQFQEALEELAKKRF---KDKSSEEAVREVHRLIEGKAPIISG 153

Query: 215 TTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAG 394
            T   +    +RLTD   +TG+HKERFD  +GKGKG  GR  L  E+   GYV GYK AG
Sbjct: 154 VTKAISSPTVSRLTDTTKFTGSHKERFD-PSGKGKGKAGRVDLVDES---GYVSGYKHAG 209

Query: 395 TYDKK 409
           TYD+K
Sbjct: 210 TYDQK 214
>sp|P59282|P25B_HUMAN Protein p25-beta
          Length = 170

 Score = 74.3 bits (181), Expect = 2e-13
 Identities = 40/70 (57%), Positives = 44/70 (62%)
 Frame = +2

Query: 200 PAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGG 379
           PA  G T  +   A  RLTD   YTG HKE FD E+GKGKGI GRE +       GYV G
Sbjct: 103 PATTGATKATTVGAVDRLTDTSKYTGTHKELFD-ESGKGKGIAGREEMTDNT---GYVSG 158

Query: 380 YKGAGTYDKK 409
           YKG+GTYDKK
Sbjct: 159 YKGSGTYDKK 168
>sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha)
          Length = 218

 Score = 73.2 bits (178), Expect = 4e-13
 Identities = 47/125 (37%), Positives = 69/125 (55%)
 Frame = +2

Query: 35  DIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHG 214
           DI F K  G + + + +  F   +E    + +   ++ + E+AV+E+   I   +P   G
Sbjct: 96  DIVFSKIKGKSCRTITFEQFKEALEELAKKRF---KDKSAEEAVREVHKLIEGKAPIISG 152

Query: 215 TTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAG 394
            T   +    +RLTD   +TG+HKERFD  +G+GKG  GR  L  E+   GYV GYK AG
Sbjct: 153 VTKAISSPTVSRLTDTSKFTGSHKERFD-PSGRGKGRAGRVDLVDES---GYVPGYKHAG 208

Query: 395 TYDKK 409
           TYD+K
Sbjct: 209 TYDQK 213
>sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I
          Length = 180

 Score = 65.1 bits (157), Expect = 1e-10
 Identities = 40/83 (48%), Positives = 47/83 (56%)
 Frame = +2

Query: 158 DAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGRE 337
           DA+ E   K+   +P+  G    +A    +RLTD   YTGAHKERFDAE GKGKG  GR 
Sbjct: 100 DAITEKLAKL--EAPSVGGAAKANAAGVYSRLTDHTKYTGAHKERFDAE-GKGKGKSGR- 155

Query: 338 YLAGENAQQGYVGGYKGAGTYDK 406
             A      GYVG YK   +YDK
Sbjct: 156 --ADTTENTGYVGAYKNKDSYDK 176
>sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893
          Length = 192

 Score = 64.3 bits (155), Expect = 2e-10
 Identities = 38/81 (46%), Positives = 48/81 (59%), Gaps = 1/81 (1%)
 Frame = +2

Query: 164 VQELKTKICQ-GSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREY 340
           + E+K K+   G+P     +   A AA  RLTD   YTG+HKERFDA +GKGKGI GR  
Sbjct: 113 LSEIKQKLASCGAPGVVSVSAGKAAAAVDRLTDTSKYTGSHKERFDA-SGKGKGIAGRRN 171

Query: 341 LAGENAQQGYVGGYKGAGTYD 403
           +   +   GYV GY+   TYD
Sbjct: 172 VVDGS---GYVSGYQHKDTYD 189
>sp|Q4L5U2|TRMD_STAHJ tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
           (tRNA [GM37] methyltransferase)
          Length = 245

 Score = 32.7 bits (73), Expect = 0.61
 Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
 Frame = -2

Query: 260 HLSNEQLPHQQKL-RLYHGRQDYLDRF*FSILVLRLHKPNFLHSYKLQ 120
           H + EQ  H+QKL R YH R D LDR+      L      F+ SYK Q
Sbjct: 199 HANIEQWRHEQKLIRTYHKRPDLLDRY-----PLTEDDEKFIESYKKQ 241
>sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
            Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28;
            p65; p210 (Papain-like proteinases 1/2)
            (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase
            (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10;
            p22; p12; Growth factor-like peptide (GFL) (p15);
            RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase
            (Hel) (p67); Unknown protein 2; p35; Unknown protein 3]
          Length = 7176

 Score = 31.6 bits (70), Expect = 1.4
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = -3

Query: 241  CRISRNSGCTMAGRTTLTDFSFQFLYCVFISQISCTHISFSHWSF 107
            CR++ +  C M+GR +LT  S+Q   C  +  ++  + +   +SF
Sbjct: 3392 CRVTSSDFCVMSGRMSLTVMSYQMQGCQLVLTVTLQNPNTPKYSF 3436
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,666,945
Number of Sequences: 369166
Number of extensions: 1077232
Number of successful extensions: 3523
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3508
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3882260660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)