Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_I02
(835 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O69060|HTXA_PSEST Probable alpha-ketoglutarate-dependent... 53 1e-06
sp|O18778|PAHX_BOVIN Phytanoyl-CoA dioxygenase, peroxisomal... 47 8e-05
sp|Q6QUY7|ECTD_STRCH Ectoine hydroxylase 44 7e-04
sp|O14832|PAHX_HUMAN Phytanoyl-CoA dioxygenase, peroxisomal... 43 0.001
sp|P57093|PAHX_RAT Phytanoyl-CoA dioxygenase, peroxisomal p... 42 0.002
sp|O62515|PAHX_CAEEL Probable phytanoyl-CoA dioxygenase (Ph... 40 0.008
sp|P67772|Y0D3_MYCTU Hypothetical protein Rv3633/MT3735 >gi... 39 0.023
sp|O35386|PAHX_MOUSE Phytanoyl-CoA dioxygenase, peroxisomal... 37 0.051
>sp|O69060|HTXA_PSEST Probable alpha-ketoglutarate-dependent hypophosphite dioxygenase
Length = 286
Score = 52.8 bits (125), Expect = 1e-06
Identities = 43/158 (27%), Positives = 68/158 (43%), Gaps = 7/158 (4%)
Frame = +1
Query: 223 PTLYVEHLNEGPIHRKTEFLAKYIFGDDMIEDFNFLITKEPFKDVPTFWHSDESYWLDLP 402
P LY + ++ K L ++ G++ I N I +P P WH D +++
Sbjct: 69 PHLYFDAIDSLVRSEKIVDLLRHFLGEN-IRLHNSKINFKPPSGAPVQWHQDWAFYPHTN 127
Query: 403 DKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPHRKYHPDKHIRTCD-------ADESE 561
D ++ + LD+T +NG M +P SHK + HR + D +E
Sbjct: 128 DD-FLTLGIFLDETSEKNGAMACLPGSHKGKVYDHRNVETGEFCHAISRSNWDEALDPTE 186
Query: 562 GVPVPLQPGELTGHGGRTLHYSRGNSTPDRRRGFVVNY 675
G + G +T H RTLH S N + RRR ++ Y
Sbjct: 187 GELLTGPVGTVTLHHVRTLHGSGPNHSTIRRRFLLIGY 224
>sp|O18778|PAHX_BOVIN Phytanoyl-CoA dioxygenase, peroxisomal precursor (Phytanoyl-CoA
alpha-hydroxylase) (PhyH) (Phytanic acid oxidase)
Length = 337
Score = 46.6 bits (109), Expect = 8e-05
Identities = 37/164 (22%), Positives = 72/164 (43%), Gaps = 8/164 (4%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKIDASIHRHDLGNINDSTKNKVENICQIM 219
++++GF+ +KN+++D DI+ + + ++ ++ L + D T K E +
Sbjct: 61 YEENGFLVIKNLVSDADIQRFRNEFERICRKEVKPL----GLSVMRDVTITKSEYVPSEK 116
Query: 220 WPTLYVEHLNEGPIHRKTEF--LAKYI---FGDDMIEDFNFLITKEPFKDVPTF---WHS 375
+ + + + R + KY+ G +++ LI K P T H
Sbjct: 117 VVSKVQDFQEDEELFRYCTLPEILKYVECFTGPNIMAMHTXLINKPPDSXKKTSRHPLHQ 176
Query: 376 DESYWLDLPDKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPH 507
D Y+ P + W A++ NGC+ +P +HK PL+PH
Sbjct: 177 DLHYFPFRPSNSIVCAWTAMEHIDRNNGCLVVLPGTHKGPLQPH 220
>sp|Q6QUY7|ECTD_STRCH Ectoine hydroxylase
Length = 297
Score = 43.5 bits (101), Expect = 7e-04
Identities = 35/154 (22%), Positives = 69/154 (44%), Gaps = 4/154 (2%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKIDASIHRHDLGNINDSTKNKVENICQIM 219
++ DGF+ V ++ ++ +Y + +S D ++ R D +I + V ++ ++
Sbjct: 45 YEHDGFLTVDQLIAPDEVAVYQAELNRLIS---DPAV-RADERSIVEKQSQNVRSVFEVH 100
Query: 220 WPTLYVEHLNEGPIH-RKTEFLAKYIFGDDMIEDFNFLITKEPFKDVPTFWHSDESYWL- 393
+ + G + + A+ I G D+ + + K F +WHSD W
Sbjct: 101 ----RISEVFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHA 156
Query: 394 --DLPDKRAMSFWVALDKTYVENGCMWFVPQSHK 489
LP+ R +S +AL + + NG + +P SHK
Sbjct: 157 EDGLPNMRTVSVSIALTENFDTNGGLMIMPGSHK 190
>sp|O14832|PAHX_HUMAN Phytanoyl-CoA dioxygenase, peroxisomal precursor (Phytanoyl-CoA
alpha-hydroxylase) (PhyH) (Phytanic acid oxidase)
Length = 338
Score = 43.1 bits (100), Expect = 0.001
Identities = 37/164 (22%), Positives = 70/164 (42%), Gaps = 8/164 (4%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKIDASIHRHDLGNINDSTKNKVENICQIM 219
++++GF+ +KN++ D DI+ + + ++ ++ L + D T +K E
Sbjct: 61 YEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPL----GLTVMRDVTISKSEYAPSEK 116
Query: 220 WPTLYVEHLNEGPIHRKTEF--LAKYI---FGDDMIEDFNFLITKEPFKDVPTF---WHS 375
T + + + R + KY+ G +++ LI K P T H
Sbjct: 117 MITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQ 176
Query: 376 DESYWLDLPDKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPH 507
D Y+ P + W A++ NGC+ +P +HK L+PH
Sbjct: 177 DLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPH 220
>sp|P57093|PAHX_RAT Phytanoyl-CoA dioxygenase, peroxisomal precursor (Phytanoyl-CoA
alpha-hydroxylase) (PhyH) (Phytanic acid oxidase)
Length = 338
Score = 42.4 bits (98), Expect = 0.002
Identities = 51/242 (21%), Positives = 93/242 (38%), Gaps = 30/242 (12%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKI---------DASIHRHDLG-------N 171
++++GF+ +KN+++D DI+ + ++ ++ D +I +
Sbjct: 61 YEENGFLVIKNLVSDDDIQRFRAEFERICRKEVKPPGMTVMKDVAIAKQGYAPSERVVTK 120
Query: 172 INDSTKNK-VENICQIMWPTLYVEHLNEGPIHRKTEFLAKYIFGDDMIEDFNFLITKEPF 348
I D +N+ + C + YVE G +++ LI K P
Sbjct: 121 IQDFQQNEELFRYCALPQIVKYVECFT----------------GPNIMAMHTMLINKPPD 164
Query: 349 KDVPTF---WHSDESYWLDLPDKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPH---- 507
T H D ++ P + W A++ NGC+ +P +HK PL+PH
Sbjct: 165 SGKKTSRHPLHQDLHFFPFRPSNLIVCAWTAMEHIDRNNGCLVVLPGTHKGPLKPHDYPK 224
Query: 508 ------RKYHPDKHIRTCDADESEGVPVPLQPGELTGHGGRTLHYSRGNSTPDRRRGFVV 669
+ YH I+ D D S V + ++ G+ +H S N T R+
Sbjct: 225 WEGGVNKMYH---GIQDYDPD-SPRVHLVMEKGDTVFFHPLLIHGSGRNRTQGFRKAISC 280
Query: 670 NY 675
+Y
Sbjct: 281 HY 282
>sp|O62515|PAHX_CAEEL Probable phytanoyl-CoA dioxygenase (Phytanoyl-CoA
alpha-hydroxylase) (PhyH) (Phytanic acid oxidase)
Length = 312
Score = 40.0 bits (92), Expect = 0.008
Identities = 49/235 (20%), Positives = 90/235 (38%), Gaps = 23/235 (9%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKIDASIHR---HDLGNINDSTKNKVENIC 210
++K+G++ ++N + ++ + + D K+ A + D+ K+ + I
Sbjct: 24 YEKNGYLLIRNCVPQYELNRFRQRFQDICEKKVKAPENMTVMKDISIAKSEFKDGEKAIT 83
Query: 211 QIMW----PTLYVEHLNEGPIHRKTEFLAKYIFGD---DMIEDFNFLITKEPFKDVPTF- 366
+I P L+ G + + K + G+ +++ LI K P T
Sbjct: 84 KIQDFADDPVLFEYCKYPGVVD-----VVKDLIGNPKSNLMAMHTMLINKPPDNGKLTSR 138
Query: 367 --WHSDESYWLDLPDKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPH----------R 510
H D Y+ P W A++K NGC+ VP +HK L PH +
Sbjct: 139 HPMHQDLQYFPFRPADFICCAWTAMEKITRANGCLVVVPGTHKGVLLPHEYPKWEGGVNK 198
Query: 511 KYHPDKHIRTCDADESEGVPVPLQPGELTGHGGRTLHYSRGNSTPDRRRGFVVNY 675
YH + T + + V ++PG+ +H S N T R+ +Y
Sbjct: 199 AYHGIQDYDT----STPRIHVEMEPGDTVFFHPILIHGSGANRTEGFRKAISCHY 249
>sp|P67772|Y0D3_MYCTU Hypothetical protein Rv3633/MT3735
sp|P67773|Y0F7_MYCBO Hypothetical protein Mb3657
Length = 291
Score = 38.5 bits (88), Expect = 0.023
Identities = 34/141 (24%), Positives = 52/141 (36%), Gaps = 1/141 (0%)
Frame = +1
Query: 256 PIHRKTEFLAKYIFGDDMIEDFNFLITKEPFKDVPTFWHSDESYWLDLPDKRAM-SFWVA 432
P+H K + + + G D + + + P DE Y L P + + + +A
Sbjct: 91 PVHPKLLPVIEGVLGRDCLLSWCMTSNQLPGAVAQRLHCDDEMYPLPRPHQPLLCNALIA 150
Query: 433 LDKTYVENGCMWFVPQSHKEPLRPHRKYHPDKHIRTCDADESEGVPVPLQPGELTGHGGR 612
L +NG VP SH+ P RP Y EG PV + G+ G
Sbjct: 151 LCDFTADNGATQVVPGSHRWPERPSPPY-------------PEGKPVEINAGDALIWNGS 197
Query: 613 TLHYSRGNSTPDRRRGFVVNY 675
H + N T R +N+
Sbjct: 198 LWHTAAANRTDAPRPALTINF 218
>sp|O35386|PAHX_MOUSE Phytanoyl-CoA dioxygenase, peroxisomal precursor (Phytanoyl-CoA
alpha-hydroxylase) (PhyH) (Phytanic acid oxidase) (Lupus
nephritis-associated peptide 1)
Length = 338
Score = 37.4 bits (85), Expect = 0.051
Identities = 34/168 (20%), Positives = 72/168 (42%), Gaps = 12/168 (7%)
Frame = +1
Query: 40 WDKDGFIHVKNILNDKDIKIYSDVYDDFLSGKI---------DASIHRHDLGNINDSTKN 192
++++GF+ +KN+++D DI+ + ++ ++ D ++ + D +D +
Sbjct: 61 YEENGFLVIKNLVSDDDIQRFRAEFERICREEVKPPGIVIMRDVALAKQDYMP-SDRMVS 119
Query: 193 KVENICQIMWPTLYVEHLNEGPIHRKTEFLAKYIFGDDMIEDFNFLITKEPFKDVPTF-- 366
K+++ + E L + + + G +++ LI K P T
Sbjct: 120 KIQDFQED-------EELFRYCLLPEILKYVECFTGPNIMALHGMLINKPPDVGKKTSRH 172
Query: 367 -WHSDESYWLDLPDKRAMSFWVALDKTYVENGCMWFVPQSHKEPLRPH 507
H D Y+ P + W A++ NGC+ +P +HK L+PH
Sbjct: 173 PLHQDLHYFPFRPSNLIVCAWTAMEHIDRNNGCLVVLPGTHKGTLKPH 220
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,489,347
Number of Sequences: 369166
Number of extensions: 2132858
Number of successful extensions: 5234
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5011
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5226
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)