Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_G08
(934 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P51885|LUM_MOUSE Lumican precursor (Keratan sulfate prot... 42 0.002
sp|Q05443|LUM_BOVIN Lumican precursor (Keratan sulfate prot... 42 0.003
sp|P51886|LUM_RAT Lumican precursor (Keratan sulfate proteo... 42 0.003
sp|P51884|LUM_HUMAN Lumican precursor (Keratan sulfate prot... 39 0.016
sp|P51890|LUM_CHICK Lumican precursor (Keratan sulfate prot... 37 0.080
sp|Q9DE67|LUM_COTJA Lumican precursor (Keratan sulfate prot... 37 0.080
sp|Q9PQV6|RPOB_UREPA DNA-directed RNA polymerase beta chain... 37 0.10
sp|O46379|LUM_RABIT Lumican (Keratan sulfate proteoglycan l... 35 0.39
sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kina... 33 1.5
sp|Q6DN56|RMAR_KLULA Mitochondrial ribosomal protein VAR1 33 1.5
>sp|P51885|LUM_MOUSE Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG
lumican)
Length = 338
Score = 42.4 bits (98), Expect = 0.002
Identities = 55/221 (24%), Positives = 100/221 (45%), Gaps = 36/221 (16%)
Frame = +2
Query: 167 LNNNDVVH----------DLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN-- 310
L NN + H DL+ ++ D N + N +I + + + K + IN N
Sbjct: 73 LRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGKVF----SKLKQLKKLHINYNNL 128
Query: 311 -DTIGHL----------KNEISKYG-INGLQ--TFVFNQNGVMLEGDD-------KTIES 427
+++G L N+ISK G +GL TF++ Q+ + E K++E
Sbjct: 129 TESVGPLPKSLQDLQLTNNKISKLGSFDGLVNLTFIYLQHNQLKEDAVSASLKGLKSLEY 188
Query: 428 LNLVNGSKIKIWRG-PNLLVTMKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESIS 604
L+L K+ G P L+T+ Y++ N + NIP+E +F+ ++ +++S
Sbjct: 189 LDLSFNQMSKLPAGLPTSLLTL--YLDNNKISNIPDEYFKRFTGLQYLRLSHNELADSGV 246
Query: 605 PGSALNLA--LITDSSYWQNDKRLIDYPIKQEGTEVFVIDL 721
PG++ N++ L D SY +L P E E + +++
Sbjct: 247 PGNSFNISSLLELDLSY----NKLKSIPTVNENLENYYLEV 283
>sp|Q05443|LUM_BOVIN Lumican precursor (Keratan sulfate proteoglycan) (KSPG) (Corneal
keratan sulfate proteoglycan 37B core protein)
Length = 342
Score = 41.6 bits (96), Expect = 0.003
Identities = 56/221 (25%), Positives = 99/221 (44%), Gaps = 36/221 (16%)
Frame = +2
Query: 167 LNNNDVVH----------DLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN-- 310
L NN + H DL+ ++ D N + N +I + + + K + IN N
Sbjct: 77 LRNNQIDHIDDKAFENVTDLQWLILDHNLLENSKIKGKVF----SKLKQLKKLHINYNNL 132
Query: 311 -DTIGHL----------KNEISKYG-INGLQ--TFVFNQNGVMLEGDD-------KTIES 427
+++G L N+ISK G +GL TF+ Q+ + E K++E
Sbjct: 133 TESVGPLPKSLVDLQLTNNKISKLGSFDGLVNLTFIHLQHNQLKEDAVSAALKGLKSLEY 192
Query: 428 LNLVNGSKIKIWRG-PNLLVTMKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESIS 604
L+L K+ G P L+T+ Y++ N + NIP+E +FS ++ +++S
Sbjct: 193 LDLSFNQMTKLPSGLPVSLLTL--YLDNNKISNIPDEYFKRFSALQYLRLSHNELADSGV 250
Query: 605 PGSALNLA--LITDSSYWQNDKRLIDYPIKQEGTEVFVIDL 721
PG++ N++ L D SY +L P E E + +++
Sbjct: 251 PGNSFNVSSLLELDLSY----NKLKSIPTVNENLENYYLEV 287
>sp|P51886|LUM_RAT Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG
lumican)
Length = 338
Score = 41.6 bits (96), Expect = 0.003
Identities = 55/223 (24%), Positives = 101/223 (45%), Gaps = 36/223 (16%)
Frame = +2
Query: 167 LNNNDVVH----------DLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN-- 310
L NN + H DL+ ++ D N + N +I + + + K + IN N
Sbjct: 73 LRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGKVF----SKLKQLKKLHINYNNL 128
Query: 311 -DTIGHL----------KNEISKYG-INGLQ--TFVFNQNGVMLEGDD-------KTIES 427
+++G L N+ISK G +GL TF++ Q+ + E K++E
Sbjct: 129 TESVGPLPKSLQDLQLANNKISKLGSFDGLVNLTFIYLQHNQLKEEAVSASLKGLKSLEY 188
Query: 428 LNLVNGSKIKIWRG-PNLLVTMKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESIS 604
L+L K+ G P L+T+ Y++ N + NIP+E +F+ ++ +++S
Sbjct: 189 LDLSFNQMSKLPAGLPTSLLTL--YLDNNKITNIPDEYFNRFTGLQYLRLSHNELADSGV 246
Query: 605 PGSALNLA--LITDSSYWQNDKRLIDYPIKQEGTEVFVIDLQR 727
PG++ N++ L D SY +L P E E + +++ +
Sbjct: 247 PGNSFNISSLLELDLSY----NKLKSIPTVNENLENYYLEVNK 285
>sp|P51884|LUM_HUMAN Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG
lumican)
Length = 338
Score = 39.3 bits (90), Expect = 0.016
Identities = 50/219 (22%), Positives = 99/219 (45%), Gaps = 32/219 (14%)
Frame = +2
Query: 167 LNNNDVVH----------DLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN-- 310
L NN + H DL+ ++ D N + N +I + + + K + IN N
Sbjct: 73 LRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGRVF----SKLKQLKKLHINHNNL 128
Query: 311 -DTIGHLK----------NEISKYG-INGLQ--TFVFNQNGVMLE----GDDKTIESLNL 436
+++G L N+I+K G GL TF+ Q+ + E K ++SL
Sbjct: 129 TESVGPLPKSLEDLQLTHNKITKLGSFEGLVNLTFIHLQHNRLKEDAVSAAFKGLKSLEY 188
Query: 437 VNGSKIKIWRGPNLLVT--MKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESISPG 610
++ S +I R P+ L + Y++ N + NIP+E +F+ ++ +++S PG
Sbjct: 189 LDLSFNQIARLPSGLPVSLLTLYLDNNKISNIPDEYFKRFNALQYLRLSHNELADSGIPG 248
Query: 611 SALNLALITDSSYWQNDKRLIDYPIKQEGTEVFVIDLQR 727
++ N++ + + N +L + P E E + +++ +
Sbjct: 249 NSFNVSSLVELDLSYN--KLKNIPTVNENLENYYLEVNQ 285
>sp|P51890|LUM_CHICK Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG
lumican)
Length = 343
Score = 37.0 bits (84), Expect = 0.080
Identities = 59/259 (22%), Positives = 107/259 (41%), Gaps = 27/259 (10%)
Frame = +2
Query: 170 NNNDVVHDLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN---DTIGHLK--- 331
N D V DL+ ++ D N + N +I + N K + IN N + +G L
Sbjct: 87 NTFDNVTDLQWLILDHNHLENSKIKGRVFSKLKNL----KKLHINYNNLTEAVGPLPKTL 142
Query: 332 -------NEISKYGINGLQ-----TFVFNQNGVM----LEGDDKTIESLNLVNGSKIKIW 463
N+I+K L+ T + QN + + G K + SL ++ S ++
Sbjct: 143 DDLQLSHNKITKVNPGALEGLVNLTVIHLQNNQLKTDSISGAFKGLNSLLYLDLSFNQLT 202
Query: 464 RGPNLLV--TMKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESISPGSALNLALIT 637
+ P L + Y + N + NIP+E F ++ ++++S PG+ N+ +
Sbjct: 203 KLPTGLPHSLLMLYFDNNQISNIPDEYFQGFKTLQYLRLSHNKLTDSGIPGNVFNITSLV 262
Query: 638 DSSYWQNDKRLIDYPIKQEGTEVFVID---LQRNPGITFGEQIKNGKYAKIFDATDSFLN 808
+ N + I P E E F + + + P +F + + Y+KI T L+
Sbjct: 263 ELDLSFNQLKSI--PTVSENLENFYLQVNKINKFPLSSFCKVVGPLTYSKI---THLRLD 317
Query: 809 RIRVNRYKLDKEIVNKRKV 865
+ R L +E+ N +V
Sbjct: 318 GNNLTRADLPQEMYNCLRV 336
>sp|Q9DE67|LUM_COTJA Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG
lumican)
Length = 343
Score = 37.0 bits (84), Expect = 0.080
Identities = 59/259 (22%), Positives = 107/259 (41%), Gaps = 27/259 (10%)
Frame = +2
Query: 170 NNNDVVHDLRSILYDENSIINKQITPVILEIRNNHFELPKWIQINSN---DTIGHLK--- 331
N D V DL+ ++ D N + N +I + N K + IN N + +G L
Sbjct: 87 NTFDNVTDLQWLILDHNHLENSKIKGRVFSKLKNL----KKLHINYNNLTEAVGPLPKTL 142
Query: 332 -------NEISKYGINGLQ-----TFVFNQNGVM----LEGDDKTIESLNLVNGSKIKIW 463
N+I+K L+ T + QN + + G K + SL ++ S ++
Sbjct: 143 DDLQLSHNKITKVNPGALEGLVNLTVIHLQNNQLKADSISGAFKGLNSLLYLDLSFNQLT 202
Query: 464 RGPNLLV--TMKFYINFNNVGNIPEELLLKFSMTDPIQDVKKRISESISPGSALNLALIT 637
+ P L + Y + N + NIP+E F ++ ++++S PG+ N+ +
Sbjct: 203 KLPTGLPHSLLMLYFDNNQISNIPDEYFQGFKTLQYLRLSHNKLTDSGIPGNVFNITSLV 262
Query: 638 DSSYWQNDKRLIDYPIKQEGTEVFVID---LQRNPGITFGEQIKNGKYAKIFDATDSFLN 808
+ N + I P E E F + + + P +F + + Y+KI T L+
Sbjct: 263 ELDLSFNQLKSI--PTVSENLENFYLQVNKINKFPLSSFCKVVGPLTYSKI---THLRLD 317
Query: 809 RIRVNRYKLDKEIVNKRKV 865
+ R L +E+ N +V
Sbjct: 318 GNNLTRADLPQEMYNCLRV 336
>sp|Q9PQV6|RPOB_UREPA DNA-directed RNA polymerase beta chain (RNAP beta subunit)
(Transcriptase beta chain) (RNA polymerase beta subunit)
Length = 1434
Score = 36.6 bits (83), Expect = 0.10
Identities = 56/251 (22%), Positives = 115/251 (45%), Gaps = 21/251 (8%)
Frame = +2
Query: 176 NDVVHDLRSILYDENSIINKQITPVIL-EIRNNHFELPKWIQINSNDTIGHLKNEISKYG 352
ND++ +IL ++++I K+ +I E R+ + + K +IN T L++EI ++
Sbjct: 423 NDIIDKKNNILIPKDTLITKEHIELIKKESRDKNIKWTK--KINLLPTA--LESEIEQF- 477
Query: 353 INGLQTFVFNQNGVMLEGDDKTIESLNLVNGSKIKIWRGPNLLVTMKFYINFN------- 511
+ V+ N + D+T E + L +G K++ +L+ T + N N
Sbjct: 478 LEYESIAVYKDNDLR----DETTEIVGLASGCKLQTLTVADLVATTSYIYNLNYEIGEFD 533
Query: 512 ---NVGNIPEEL---LLKFSMTDPIQDVKKRISE--SISPGSALNLALITDSSYWQNDKR 667
++GN +L LL+ + + ++K I+E +IS GS+ N+ + D
Sbjct: 534 DIDHLGNKRLKLIHELLRARIATSMARIEKFINEKLAISDGSSNNITNVNDKG------- 586
Query: 668 LIDYPIKQEGTEVFVIDLQRNPGITFGEQIKNGKYAKIFDATDSFLNRIRVNRYKLDK-- 841
ID + +E E + D ++ I+ I ++ + F N ++ ++ +D+
Sbjct: 587 -IDTELDREIEESDMSDEEKKKAISVKSIINTKQFQSL---VKDFFNSHQLIQF-IDQQN 641
Query: 842 ---EIVNKRKV 865
E+ NKR++
Sbjct: 642 PLAELTNKRRI 652
>sp|O46379|LUM_RABIT Lumican (Keratan sulfate proteoglycan lumican) (KSPG lumican)
Length = 192
Score = 34.7 bits (78), Expect = 0.39
Identities = 43/190 (22%), Positives = 87/190 (45%), Gaps = 21/190 (11%)
Frame = +2
Query: 215 ENSIINKQITPVILEIRNNHFELPKWIQINSNDTIGHLK----------NEISKYG-ING 361
ENS I ++ + +++ H N +++G L N+I+K G +G
Sbjct: 12 ENSKIKGKVFSKLKQLKKLHIN-----HNNLTESVGPLPKSLEDLQLTHNKITKLGSFDG 66
Query: 362 LQ--TFVFNQNGVMLEGDD-------KTIESLNLVNGSKIKIWRG-PNLLVTMKFYINFN 511
L TFV Q+ + E K++E L+L K+ G P L+T+ Y++ N
Sbjct: 67 LLNLTFVHLQHNQLKEDAVSAAFKGLKSLEYLDLSFNQMSKLPSGLPASLLTL--YLDNN 124
Query: 512 NVGNIPEELLLKFSMTDPIQDVKKRISESISPGSALNLALITDSSYWQNDKRLIDYPIKQ 691
+ +IP+E +F+ ++ +++S PG++ N++ + + N +L + P
Sbjct: 125 KISDIPDEYFKRFNGLQYLRLSHNELADSGIPGNSFNISSLVELDLSYN--KLKNIPTVN 182
Query: 692 EGTEVFVIDL 721
E E + +++
Sbjct: 183 ENLENYYLEV 192
>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
Length = 1081
Score = 32.7 bits (73), Expect = 1.5
Identities = 20/57 (35%), Positives = 31/57 (54%)
Frame = +2
Query: 743 FGEQIKNGKYAKIFDATDSFLNRIRVNRYKLDKEIVNKRKVLKYAIISHTLLNRINN 913
FG I +G Y+ + AT S + R L+KE + ++K +KY I T L ++NN
Sbjct: 181 FGSVIGDGAYSTVMLAT-SIDTKKRYAAKVLNKEYLIRQKKVKYVSIEKTALQKLNN 236
>sp|Q6DN56|RMAR_KLULA Mitochondrial ribosomal protein VAR1
Length = 388
Score = 32.7 bits (73), Expect = 1.5
Identities = 59/235 (25%), Positives = 96/235 (40%), Gaps = 16/235 (6%)
Frame = +2
Query: 116 INVDTRRIQFYDERLSLLNNNDVVHDLRSILYDENSIINKQITPVILEIRNNHFELPKWI 295
IN+ I LSL N DV + + Y+ +N I L+I N+ +
Sbjct: 167 INLLNNNINSISNILSLYYNKDVSFEPIQLKYN---YMNSDILAKSLKIMNSSSK----- 218
Query: 296 QINSNDTIGHLKNEISKYGINGL-QTFVFNQNGVMLEGDDKTIESLNLVNG-----SKIK 457
+ S + I +L N + K + QT++ N N +M + I+S+N N S
Sbjct: 219 RSLSMNRIKNLMNNMPKLNDKLISQTYINNINNIMFNKYNNIIKSINNNNNNNPADSSPG 278
Query: 458 IWRGPNLLVTMKFYINFNNVGNIPEELLL-KFSMTDPIQDVKKRIS--ESISPGSALNLA 628
RG NN+ IP +L+ K+ + IQ +K R+S +SIS + +N+
Sbjct: 279 NPRGGAAGKLYNIIYENNNINTIPNNILMFKYLVGWSIQ-LKGRLSNNKSISRSNTMNIL 337
Query: 629 LITDSSY---W----QNDKRLIDYPIKQEGTEVFVIDLQRNPGITFGEQIKNGKY 772
T +Y W N+ +L P+ T + ++ KNGKY
Sbjct: 338 NGTFQNYKYIWGNQMVNNYKLNYIPVNHNITNISDVN-------------KNGKY 379
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,338,045
Number of Sequences: 369166
Number of extensions: 2032639
Number of successful extensions: 5440
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5438
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9606826000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)