Planarian EST Database


Dr_sW_012_G04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_012_G04
         (443 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q13435|SF3B2_HUMAN  Splicing factor 3B subunit 2 (Spliceo...   217   8e-57
sp|Q02554|CUS1_YEAST  Protein CUS1                                 95   6e-20
sp|Q5R789|ZCHC8_PONPY  Zinc finger CCHC domain containing pr...    41   0.001
sp|Q9CYA6|ZCHC8_MOUSE  Zinc finger CCHC domain containing pr...    41   0.001
sp|Q6NZY4|ZCHC8_HUMAN  Zinc finger CCHC domain containing pr...    41   0.001
sp|Q5F3D1|ZCHC8_CHICK  Zinc finger CCHC domain containing pr...    38   0.011
sp|P30415|NKTR_MOUSE  NK-tumor recognition protein (Natural-...    33   0.28 
sp|Q9FFK1|SY31_ARATH  Syntaxin 31 (AtSYP31) (AtSED5)               32   0.62 
sp|P30414|NKTR_HUMAN  NK-tumor recognition protein (Natural-...    31   1.1  
sp|Q05859|FMN1B_MOUSE  Formin-1 isoform IV (Limb deformity p...    30   1.8  
>sp|Q13435|SF3B2_HUMAN Splicing factor 3B subunit 2 (Spliceosome associated protein 145)
           (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145
           kDa subunit)
          Length = 872

 Score =  217 bits (553), Expect = 8e-57
 Identities = 96/147 (65%), Positives = 121/147 (82%)
 Frame = +3

Query: 3   FKLPDFIAKTGIMEMRQTADDKESEKSLKNKMKDKIRPKVGLVDISYEKLYNAFFVEQTK 182
           F+LPDFI +TGI EMR+   +KE +K++K+KM++K+RPK+G +DI Y+KL++AFF  QTK
Sbjct: 499 FELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTK 558

Query: 183 PELTKFGDIYYQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGP 362
           P+LT  GD+YY+GKE E + KE +PG LSDELR++LGM  GPN+ K PPPWLIA QRYGP
Sbjct: 559 PKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGP 618

Query: 363 PPSYPNLKIPGLNKGIPPGCSFGFHAG 443
           PPSYPNLKIPGLN  IP  CSFG+HAG
Sbjct: 619 PPSYPNLKIPGLNSPIPESCSFGYHAG 645
>sp|Q02554|CUS1_YEAST Protein CUS1
          Length = 436

 Score = 95.1 bits (235), Expect = 6e-20
 Identities = 56/146 (38%), Positives = 80/146 (54%), Gaps = 13/146 (8%)
 Frame = +3

Query: 3   FKLPDFIAKTGIMEMRQTAD----DKESEKSLKNKMKDKIRPKVGLVDISYEKLYNAFFV 170
           F+LPD I KT I +MR T      D + EKSLK   + +++PK+G +D+ Y+KL++ FF 
Sbjct: 194 FELPDIIKKTNIEQMRSTLPQSGLDGQDEKSLKEASRARVQPKMGALDLDYKKLHDVFFK 253

Query: 171 --EQTKPE-LTKFGDIYYQGKELEAKRK------ENRPGLLSDELRVALGMTTGPNSDKS 323
                KP+ L  FGD+YY+ + L  +          RPG +S ELR  + +  G      
Sbjct: 254 IGANWKPDHLLCFGDVYYENRNLFEETNWKRMVDHKRPGRISQELRAIMNLPEG-----Q 308

Query: 324 PPPWLIAQQRYGPPPSYPNLKIPGLN 401
            PPW +  +  G P  YP+LKI GLN
Sbjct: 309 LPPWCMKMKDIGLPTGYPDLKIAGLN 334
>sp|Q5R789|ZCHC8_PONPY Zinc finger CCHC domain containing protein 8
          Length = 704

 Score = 40.8 bits (94), Expect = 0.001
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 213 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 374
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 272 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 320
>sp|Q9CYA6|ZCHC8_MOUSE Zinc finger CCHC domain containing protein 8
          Length = 709

 Score = 40.8 bits (94), Expect = 0.001
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 213 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 374
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 277 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 325
>sp|Q6NZY4|ZCHC8_HUMAN Zinc finger CCHC domain containing protein 8
          Length = 707

 Score = 40.8 bits (94), Expect = 0.001
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +3

Query: 213 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 374
           Y  +E+E +    +PG++S+EL+ ALG+T     DKS PP++   ++ G PP +
Sbjct: 274 YHAEEVEERFGRFKPGVISEELQDALGVT-----DKSLPPFIYRMRQLGYPPGW 322
>sp|Q5F3D1|ZCHC8_CHICK Zinc finger CCHC domain containing protein 8
          Length = 613

 Score = 37.7 bits (86), Expect = 0.011
 Identities = 19/54 (35%), Positives = 33/54 (61%)
 Frame = +3

Query: 213 YQGKELEAKRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSY 374
           Y  +E+E +  + +PG++S EL+ ALG+T      KS PP++   ++ G PP +
Sbjct: 173 YHAEEVEERFGKFKPGVISGELQDALGVTA-----KSLPPFIYRMRQLGYPPGW 221
>sp|P30415|NKTR_MOUSE NK-tumor recognition protein (Natural-killer cells
            cyclophilin-related protein) (NK-TR protein)
          Length = 1453

 Score = 33.1 bits (74), Expect = 0.28
 Identities = 24/101 (23%), Positives = 46/101 (45%), Gaps = 1/101 (0%)
 Frame = +3

Query: 24   AKTGIMEMRQTADDKESEKSLKNKMKDKIRPKVGLVDISYEKLYNAFFVEQTKPELTKFG 203
            + T +    +T+  KE  +  +  + DK +P  G+ ++S      +  ++     L+   
Sbjct: 1189 SSTSVTSPVETSGKKEGAEKSQMNLTDKWKPLQGVGNLSVSTATTSSALDVKA--LSTVP 1246

Query: 204  DIYYQGKELEAKRKEN-RPGLLSDELRVALGMTTGPNSDKS 323
            ++  QG  +E K K   RPG L DE+R    +   P + +S
Sbjct: 1247 EVKPQGLRIEIKSKNKVRPGSLFDEVRKTARLNRRPRNQES 1287
>sp|Q9FFK1|SY31_ARATH Syntaxin 31 (AtSYP31) (AtSED5)
          Length = 336

 Score = 32.0 bits (71), Expect = 0.62
 Identities = 20/61 (32%), Positives = 25/61 (40%)
 Frame = +3

Query: 237 KRKENRPGLLSDELRVALGMTTGPNSDKSPPPWLIAQQRYGPPPSYPNLKIPGLNKGIPP 416
           K  ENR  L S +  V         S   PPPW  +   +G   +     +P LN G PP
Sbjct: 157 KAHENRKQLFSTKNAVDSPPQNNAKSVPEPPPWSSSSNPFG---NLQQPLLPPLNTGAPP 213

Query: 417 G 419
           G
Sbjct: 214 G 214
>sp|P30414|NKTR_HUMAN NK-tumor recognition protein (Natural-killer cells
            cyclophilin-related protein) (NK-TR protein)
          Length = 1462

 Score = 31.2 bits (69), Expect = 1.1
 Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 1/91 (1%)
 Frame = +3

Query: 54   TADDKESEKSLKNKMKDKIRPKVGLVDISYEKLYNAFFVEQTKPELTKFGDIYYQGKELE 233
            T   + +EKS  N +  K +P  G+ +++      +  VE     LT   ++  QG  +E
Sbjct: 1208 TGKKEVAEKSQINLIDKKWKPLQGVGNLAAPNAATSSAVEVKV--LTTVPEMKPQGLRIE 1265

Query: 234  AKRKEN-RPGLLSDELRVALGMTTGPNSDKS 323
             K K   RPG L DE+R    +   P + +S
Sbjct: 1266 IKSKNKVRPGSLFDEVRKTARLNRRPRNQES 1296
>sp|Q05859|FMN1B_MOUSE Formin-1 isoform IV (Limb deformity protein)
          Length = 1206

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 21/48 (43%), Positives = 22/48 (45%), Gaps = 5/48 (10%)
 Frame = +3

Query: 303 GPNSDKSPPPWL---IAQQRYG--PPPSYPNLKIPGLNKGIPPGCSFG 431
           GP     PPP L   +A    G  PPP  P    PGL    PPG SFG
Sbjct: 702 GPPPPPPPPPPLPNVLALPNSGGPPPPPPPPPPPPGLAPPPPPGLSFG 749
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,718,211
Number of Sequences: 369166
Number of extensions: 1084421
Number of successful extensions: 3572
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3318
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3543
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2344429560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)