Planarian EST Database


Dr_sW_012_F05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_012_F05
         (456 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P35782|VA52_VESCR  Venom allergen 5.02 (Antigen 5-2) (Ag5...   103   2e-22
sp|O19010|CRIS3_HORSE  Cysteine-rich secretory protein 3 pre...   102   5e-22
sp|P54108|CRIS3_HUMAN  Cysteine-rich secretory protein 3 pre...   100   1e-21
sp|P35781|VA51_VESCR  Venom allergen 5.01 (Antigen 5-1) (Ag5...   100   3e-21
sp|P35783|VA5_VESFL  Venom allergen 5 (Antigen 5) (Ag5) (All...   100   3e-21
sp|P35760|VA5_VESMC  Venom allergen 5 (Antigen 5) (Ag5) (All...    99   3e-21
sp|P81657|VA5_VESMA  Venom allergen 5 (Antigen 5) (Ag5) (All...    99   4e-21
sp|Q60477|CRIS2_CAVPO  Cysteine-rich secretory protein 2 pre...    98   1e-20
sp|P16562|CRIS2_HUMAN  Cysteine-rich secretory protein 2 pre...    97   1e-20
sp|Q05110|VA5_VESVU  Venom allergen 5 precursor (Antigen 5) ...    97   2e-20
>sp|P35782|VA52_VESCR Venom allergen 5.02 (Antigen 5-2) (Ag5-2) (Allergen Vesp c 5.02)
           (Vesp c V.02)
          Length = 202

 Score =  103 bits (256), Expect = 2e-22
 Identities = 55/125 (44%), Positives = 74/125 (59%), Gaps = 5/125 (4%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFD 170
           WN+E AQ AQ +AN+C +GHD  ++    S   VGQNIA  S++     +V+  ++MW D
Sbjct: 79  WNDELAQIAQVWANQCNYGHDNCRNSAKYS---VGQNIAEGSTSADNFVNVSNMVKMWED 135

Query: 171 EVKGYTFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNN 350
           EVK Y + + +       HYT +VWAKTKE GCG  D       + +L+CNYGP GN  N
Sbjct: 136 EVKDYQYGSPKNKLNKVGHYTQMVWAKTKEIGCGSEDYIEDGWHRHYLVCNYGPAGNVGN 195

Query: 351 EKPYE 365
           E  YE
Sbjct: 196 EPIYE 200
>sp|O19010|CRIS3_HORSE Cysteine-rich secretory protein 3 precursor (CRISP-3)
          Length = 245

 Score =  102 bits (253), Expect = 5e-22
 Identities = 51/145 (35%), Positives = 82/145 (56%), Gaps = 4/145 (2%)
 Frame = +3

Query: 3   KWNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWS-SNDVTEGIQMWFDEVK 179
           +W+++ A  AQN+AN+C+  H +++DR  G+    G+N+   S  N  ++ IQ W DEV 
Sbjct: 63  QWDSKTATNAQNWANKCLLQHSKAEDRAVGTMK-CGENLFMSSIPNSWSDAIQNWHDEVH 121

Query: 180 GYTFN-NDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNE- 353
            + +    +       HYT +VW  +   GCGIA C +Q   K + +C Y P GN+ N+ 
Sbjct: 122 DFKYGVGPKTPNAVVGHYTQVVWYSSYRVGCGIAYCPKQGTLKYYYVCQYCPAGNYVNKI 181

Query: 354 -KPYETGSACSKCPSGTRCNSSLCS 425
             PYE G+ C++CP    C++ LC+
Sbjct: 182 NTPYEQGTPCARCPG--NCDNGLCT 204
>sp|P54108|CRIS3_HUMAN Cysteine-rich secretory protein 3 precursor (CRISP-3) (SGP28
           protein)
          Length = 245

 Score =  100 bits (250), Expect = 1e-21
 Identities = 54/148 (36%), Positives = 73/148 (49%), Gaps = 7/148 (4%)
 Frame = +3

Query: 3   KWNNEAAQRAQNYANRCVFGHDQSKDR----KFGSYDWVGQNIAYWSSNDVTEGIQMWFD 170
           +WN EAA  AQ +AN+C + H   KDR    K G   ++    + WS     + IQ WFD
Sbjct: 64  EWNKEAAANAQKWANQCNYRHSNPKDRMTSLKCGENLYMSSASSSWS-----QAIQSWFD 118

Query: 171 EVKGYTFN-NDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWN 347
           E   + F    +       HYT +VW  +   GCG A C  Q + K + +C Y P GNW 
Sbjct: 119 EYNDFDFGVGPKTPNAVVGHYTQVVWYSSYLVGCGNAYCPNQKVLKYYYVCQYCPAGNWA 178

Query: 348 NE--KPYETGSACSKCPSGTRCNSSLCS 425
           N    PYE G+ C+ CP    C+  LC+
Sbjct: 179 NRLYVPYEQGAPCASCPD--NCDDGLCT 204
>sp|P35781|VA51_VESCR Venom allergen 5.01 (Antigen 5-1) (Ag5-1) (Allergen Vesp c 5.01)
           (Vesp c V.01)
          Length = 202

 Score = 99.8 bits (247), Expect = 3e-21
 Identities = 54/125 (43%), Positives = 71/125 (56%), Gaps = 5/125 (4%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFD 170
           WN+E AQ AQ +AN+C +GHD  ++    S   VGQNIA  S+       V+  ++MW D
Sbjct: 79  WNDELAQIAQVWANQCNYGHDNCRNSAKYS---VGQNIAEGSTTADNFGSVSNMVKMWED 135

Query: 171 EVKGYTFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNN 350
           EVK Y + + +       HYT +VWAKTKE GCG          + +L+CNYGP GN  N
Sbjct: 136 EVKDYQYGSPKNKLNKVGHYTQMVWAKTKEIGCGSIKYIENGWHRHYLVCNYGPAGNVGN 195

Query: 351 EKPYE 365
           E  YE
Sbjct: 196 EPIYE 200
>sp|P35783|VA5_VESFL Venom allergen 5 (Antigen 5) (Ag5) (Allergen Ves f 5) (Ves f V)
          Length = 204

 Score = 99.8 bits (247), Expect = 3e-21
 Identities = 57/132 (43%), Positives = 76/132 (57%), Gaps = 11/132 (8%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSS-----NDVTEGIQMWFD 170
           WN+E A  AQ +AN+C +GHD  +D     Y  VGQN+A   S     +D  + ++MW D
Sbjct: 79  WNDELAYVAQVWANQCQYGHDTCRD--IAKYQ-VGQNVALTGSTAAKYDDPVKLVKMWED 135

Query: 171 EVKGY------TFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGP 332
           EVK Y      + NN   TG    HYT +VWA TKE GCG     ++   K +L+CNYGP
Sbjct: 136 EVKDYNPKKKFSGNNFLKTG----HYTQMVWANTKEVGCGSIKFIQEKWHKHYLVCNYGP 191

Query: 333 GGNWNNEKPYET 368
            GN+ NE+ Y+T
Sbjct: 192 SGNFQNEELYQT 203
>sp|P35760|VA5_VESMC Venom allergen 5 (Antigen 5) (Ag5) (Allergen Ves m 5) (Ves m V)
          Length = 204

 Score = 99.4 bits (246), Expect = 3e-21
 Identities = 57/132 (43%), Positives = 76/132 (57%), Gaps = 11/132 (8%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSS-----NDVTEGIQMWFD 170
           W++E A  AQ +AN+C +GHD  +D     Y  VGQN+A   S     ND  + ++MW D
Sbjct: 79  WSDELAYIAQVWANQCQYGHDTCRD--VAKYQ-VGQNVALTGSTAAVYNDPVKLVKMWED 135

Query: 171 EVKGY------TFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGP 332
           EVK Y      + NN    G    HYT +VWA TKE GCG     ++N  K +L+CNYGP
Sbjct: 136 EVKDYNPKKKFSENNFLKIG----HYTQMVWANTKEVGCGSIKYIQENWHKHYLVCNYGP 191

Query: 333 GGNWNNEKPYET 368
            GN+ NE+ Y+T
Sbjct: 192 SGNFQNEELYQT 203
>sp|P81657|VA5_VESMA Venom allergen 5 (Antigen 5) (Ag5) (Allergen Vesp m 5)
          Length = 202

 Score = 99.0 bits (245), Expect = 4e-21
 Identities = 54/125 (43%), Positives = 69/125 (55%), Gaps = 5/125 (4%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFD 170
           WN+E AQ AQ +A +C +GHD  ++    S   VGQNIA   S       V+  +QMW D
Sbjct: 79  WNDELAQIAQVWAGQCDYGHDVCRNTAKYS---VGQNIAENGSTAASFASVSNMVQMWAD 135

Query: 171 EVKGYTFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNN 350
           EVK Y + + +       HYT +VWAKTKE GCG          + +L+CNYGP GN  N
Sbjct: 136 EVKNYQYGSTKNKLIEVGHYTQMVWAKTKEIGCGSIKYIENGWHRHYLVCNYGPAGNIGN 195

Query: 351 EKPYE 365
           E  YE
Sbjct: 196 EPIYE 200
>sp|Q60477|CRIS2_CAVPO Cysteine-rich secretory protein 2 precursor (CRISP-2)
           (Testis-specific protein TPX-1) (Autoantigen 1) (25 kDa
           acrosomal autoantigen) (AA1)
          Length = 244

 Score = 97.8 bits (242), Expect = 1e-20
 Identities = 55/146 (37%), Positives = 76/146 (52%), Gaps = 5/146 (3%)
 Frame = +3

Query: 3   KWNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSNDVT--EGIQMWFDEV 176
           +W+ EAA  AQ +ANRC   H    DRK  +    G+N+ Y SS+  +  + IQ WFDE 
Sbjct: 63  EWSREAAVNAQKWANRCTLVHSNPDDRKTSTK--CGENL-YMSSDPSSWSDAIQSWFDES 119

Query: 177 KGYTFN-NDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGN--WN 347
           + +TF    +       HYT LVW  +   GCGIA C  Q+  K + +C Y P GN  + 
Sbjct: 120 QDFTFGVGPKSHNAVVGHYTQLVWYSSYLVGCGIAYCPNQDSLKYYYVCQYCPAGNNVYT 179

Query: 348 NEKPYETGSACSKCPSGTRCNSSLCS 425
              PY+ G  C+ CP    C + LC+
Sbjct: 180 KNTPYKQGIPCASCPG--HCENGLCT 203
>sp|P16562|CRIS2_HUMAN Cysteine-rich secretory protein 2 precursor (CRISP-2)
           (Testis-specific protein TPX-1)
          Length = 243

 Score = 97.4 bits (241), Expect = 1e-20
 Identities = 52/146 (35%), Positives = 76/146 (52%), Gaps = 5/146 (3%)
 Frame = +3

Query: 3   KWNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSNDVT--EGIQMWFDEV 176
           +W+ E    AQ +AN+C   H   +DRK  +    G+N+ Y SS+  +    IQ W+DE+
Sbjct: 62  EWSREVTTNAQRWANKCTLQHSDPEDRKTSTR--CGENL-YMSSDPTSWSSAIQSWYDEI 118

Query: 177 KGYTFN-NDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNE 353
             + +    +       HYT LVW  T + GCGIA C  Q+  K + +C Y P GN  N 
Sbjct: 119 LDFVYGVGPKSPNAVVGHYTQLVWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNR 178

Query: 354 K--PYETGSACSKCPSGTRCNSSLCS 425
           K  PY+ G+ C+ CP    C+  LC+
Sbjct: 179 KNTPYQQGTPCAGCPDD--CDKGLCT 202
>sp|Q05110|VA5_VESVU Venom allergen 5 precursor (Antigen 5) (Ag5) (Allergen Ves v 5)
           (Ves v V)
          Length = 227

 Score = 96.7 bits (239), Expect = 2e-20
 Identities = 55/129 (42%), Positives = 75/129 (58%), Gaps = 8/129 (6%)
 Frame = +3

Query: 6   WNNEAAQRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSS-----NDVTEGIQMWFD 170
           WN+E A  AQ +AN+C +GHD  +D     Y  VGQN+A   S     +D  + ++MW D
Sbjct: 102 WNDELAYVAQVWANQCQYGHDTCRD--VAKYQ-VGQNVALTGSTAAKYDDPVKLVKMWED 158

Query: 171 EVKGYTFNNDRCTGFTCL---HYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGN 341
           EVK Y     + +G   L   HYT +VWA TKE GCG     ++   K +L+CNYGP GN
Sbjct: 159 EVKDYN-PKKKFSGNDFLKTGHYTQMVWANTKEVGCGSIKYIQEKWHKHYLVCNYGPSGN 217

Query: 342 WNNEKPYET 368
           + NE+ Y+T
Sbjct: 218 FMNEELYQT 226
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,654,728
Number of Sequences: 369166
Number of extensions: 1185612
Number of successful extensions: 3090
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2946
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2484837250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)