Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_E11
(578 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35286|DHX15_MOUSE Putative pre-mRNA splicing factor RNA... 179 3e-45
sp|O43143|DHX15_HUMAN Putative pre-mRNA splicing factor RNA... 179 3e-45
sp|O17438|DHX15_STRPU Putative pre-mRNA splicing factor ATP... 167 2e-41
sp|Q20875|DHX15_CAEEL Putative pre-mRNA splicing factor ATP... 162 7e-40
sp|O22899|DHX15_ARATH Putative pre-mRNA splicing factor ATP... 150 2e-36
sp|O42945|DHX15_SCHPO Probable pre-mRNA splicing factor RNA... 117 2e-26
sp|P53131|PRP43_YEAST Pre-mRNA splicing factor RNA helicase... 113 4e-25
sp|O42643|DHX8_SCHPO Putative pre-mRNA splicing factor ATP-... 101 2e-21
sp|Q14562|DHX8_HUMAN ATP-dependent helicase DHX8 (RNA helic... 97 3e-20
sp|Q92620|PRP16_HUMAN Pre-mRNA splicing factor ATP-dependen... 97 4e-20
>sp|O35286|DHX15_MOUSE Putative pre-mRNA splicing factor RNA helicase (DEAH box protein
15)
Length = 795
Score = 179 bits (455), Expect = 3e-45
Identities = 99/148 (66%), Positives = 109/148 (73%), Gaps = 9/148 (6%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
+LT+LGSMMAEFPLDPQLAKMVIASCD+NCSNE+LSITAMLSVPQCFVRPTEA+ AADEA
Sbjct: 554 DLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEA 613
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDSQWCYDISSITDL*RCR*CPDSVVQNHGSIQPS 361
KM FAHIDGDHLT+LNVYHAFKQN E QWCYD + I R D+V Q Q S
Sbjct: 614 KMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYD-NFINY--RSLMSADNVRQ-----QLS 665
Query: 362 SVQHRFQLD---------GYYVNIRKRL 418
+ RF L YY+NIRK L
Sbjct: 666 RIMDRFNLPRRSTDFTSRDYYINIRKAL 693
Score = 110 bits (275), Expect = 3e-24
Identities = 60/105 (57%), Positives = 69/105 (65%), Gaps = 9/105 (8%)
Frame = +1
Query: 283 FVDYRSLTLP---------IMSGFSCPESWIDSTFVGPAPISARRILCKHQKALVSGFFM 435
F++YRSL IM F+ P D T +R +KALV+G+FM
Sbjct: 648 FINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFT--------SRDYYINIRKALVTGYFM 699
Query: 436 QVAHLERSGHYLTVKDNQIVHLHPSTCLDHKPEWVLYNEFVLTTK 570
QVAHLER+GHYLTVKDNQ+V LHPST LDHKPEWVLYNEFVLTTK
Sbjct: 700 QVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTK 744
>sp|O43143|DHX15_HUMAN Putative pre-mRNA splicing factor RNA helicase (DEAH box protein
15) (ATP-dependent RNA helicase #46)
Length = 795
Score = 179 bits (455), Expect = 3e-45
Identities = 99/148 (66%), Positives = 109/148 (73%), Gaps = 9/148 (6%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
+LT+LGSMMAEFPLDPQLAKMVIASCD+NCSNE+LSITAMLSVPQCFVRPTEA+ AADEA
Sbjct: 554 DLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEA 613
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDSQWCYDISSITDL*RCR*CPDSVVQNHGSIQPS 361
KM FAHIDGDHLT+LNVYHAFKQN E QWCYD + I R D+V Q Q S
Sbjct: 614 KMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYD-NFINY--RSLMSADNVRQ-----QLS 665
Query: 362 SVQHRFQLD---------GYYVNIRKRL 418
+ RF L YY+NIRK L
Sbjct: 666 RIMDRFNLPRRSTDFTSRDYYINIRKAL 693
Score = 110 bits (275), Expect = 3e-24
Identities = 60/105 (57%), Positives = 69/105 (65%), Gaps = 9/105 (8%)
Frame = +1
Query: 283 FVDYRSLTLP---------IMSGFSCPESWIDSTFVGPAPISARRILCKHQKALVSGFFM 435
F++YRSL IM F+ P D T +R +KALV+G+FM
Sbjct: 648 FINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFT--------SRDYYINIRKALVTGYFM 699
Query: 436 QVAHLERSGHYLTVKDNQIVHLHPSTCLDHKPEWVLYNEFVLTTK 570
QVAHLER+GHYLTVKDNQ+V LHPST LDHKPEWVLYNEFVLTTK
Sbjct: 700 QVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTK 744
>sp|O17438|DHX15_STRPU Putative pre-mRNA splicing factor ATP-dependent RNA helicase PRP1
Length = 455
Score = 167 bits (422), Expect = 2e-41
Identities = 78/93 (83%), Positives = 85/93 (91%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
+LT LGSMMAEFPLDPQLAKMVIAS D++CSNEILS+TAMLSVPQCF+RP EA+ ADEA
Sbjct: 322 DLTRLGSMMAEFPLDPQLAKMVIASTDYSCSNEILSVTAMLSVPQCFLRPNEAKKLADEA 381
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDSQWCYD 280
KM FAHIDGDHLT+LNVYHAFKQN ED QWCYD
Sbjct: 382 KMRFAHIDGDHLTLLNVYHAFKQNNEDPQWCYD 414
Score = 30.4 bits (67), Expect = 3.3
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = +3
Query: 306 AADNVRIQLSRIMDRFNLRR 365
+AD+VR QL+RIMDRF L+R
Sbjct: 424 SADSVRQQLARIMDRFALQR 443
>sp|Q20875|DHX15_CAEEL Putative pre-mRNA splicing factor ATP-dependent RNA helicase
F56D2.6
Length = 739
Score = 162 bits (409), Expect = 7e-40
Identities = 88/142 (61%), Positives = 101/142 (71%), Gaps = 4/142 (2%)
Frame = +2
Query: 5 LTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEAK 184
LT+LGS+MAEFPLDPQLAKM+I S + NCSNEILSITAMLSVPQC+VRP E RT ADEAK
Sbjct: 499 LTELGSLMAEFPLDPQLAKMLITSTELNCSNEILSITAMLSVPQCWVRPNEMRTEADEAK 558
Query: 185 MGFAHIDGDHLTMLNVYHAFKQNREDSQWCYDISSITDL*RCR*CPDSVVQNHGSIQPS- 361
FAHIDGDHLT+LNVYH+FKQN+ED QWCYD + I R D+V +
Sbjct: 559 ARFAHIDGDHLTLLNVYHSFKQNQEDPQWCYD-NFINY--RTMKTADTVRTQLSRVMDKY 615
Query: 362 ---SVQHRFQLDGYYVNIRKRL 418
V F+ YY+NIRK L
Sbjct: 616 NLRRVSTDFKSRDYYLNIRKAL 637
Score = 106 bits (265), Expect = 4e-23
Identities = 48/55 (87%), Positives = 53/55 (96%)
Frame = +1
Query: 406 QKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKPEWVLYNEFVLTTK 570
+KALV+GFFMQVAHLERSGHY+TVKDNQ+V+LHPST LDHKPEW LYNEFVLTTK
Sbjct: 634 RKALVAGFFMQVAHLERSGHYVTVKDNQLVNLHPSTVLDHKPEWALYNEFVLTTK 688
>sp|O22899|DHX15_ARATH Putative pre-mRNA splicing factor ATP-dependent RNA helicase
Length = 729
Score = 150 bits (379), Expect = 2e-36
Identities = 81/148 (54%), Positives = 100/148 (67%), Gaps = 9/148 (6%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
NLT G +M+EFPLDPQ++KM+I S +FNCSNEILS++AMLSVP CFVRP EA+ AADEA
Sbjct: 485 NLTKTGEIMSEFPLDPQMSKMLIVSPEFNCSNEILSVSAMLSVPNCFVRpreAQKAADEA 544
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDSQWCYDISSITDL*RCR*CPDSVVQNHGSIQPS 361
K F HIDGDHLT+LNVYHA+KQN ED WC++ + + + R D+V Q Q
Sbjct: 545 KARFGHIDGDHLTLLNVYHAYKQNNEDPNWCFE-NFVNN--RAMKSADNVRQ-----QLV 596
Query: 362 SVQHRFQLD---------GYYVNIRKRL 418
+ RF L YYVNIRK +
Sbjct: 597 RIMSRFNLKMCSTDFNSRDYYVNIRKAM 624
Score = 107 bits (266), Expect = 3e-23
Identities = 44/55 (80%), Positives = 54/55 (98%)
Frame = +1
Query: 406 QKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKPEWVLYNEFVLTTK 570
+KA+++G+FMQVAHLER+GHYLTVKDNQ+VHLHPS CLDHKPEWV+YNE+VLTT+
Sbjct: 621 RKAMLAGYFMQVAHLERTGHYLTVKDNQVVHLHPSNCLDHKPEWVIYNEYVLTTR 675
>sp|O42945|DHX15_SCHPO Probable pre-mRNA splicing factor RNA helicase prp43
Length = 735
Score = 117 bits (293), Expect = 2e-26
Identities = 54/93 (58%), Positives = 67/93 (72%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
+LT LG +EFPLDP LA M+I S +F CSNE+LS+TA+LSVP FVRP AR ADE
Sbjct: 491 DLTPLGRKASEFPLDPNLAVMLIRSPEFYCSNEVLSLTALLSVPNVFVRPNSARKLADEM 550
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDSQWCYD 280
+ F H DGDHLT+LNVYHA+K + WC++
Sbjct: 551 RQQFTHPDGDHLTLLNVYHAYKSGEGTADWCWN 583
Score = 75.5 bits (184), Expect = 9e-14
Identities = 38/67 (56%), Positives = 47/67 (70%), Gaps = 1/67 (1%)
Frame = +1
Query: 373 PISARRILCKHQKALVSGFFMQVAHLERSG-HYLTVKDNQIVHLHPSTCLDHKPEWVLYN 549
P + ++ALVSGFFMQVA +G +Y+T+KDNQ+V LHPS L PEWV+YN
Sbjct: 616 PFDDKNYYVNIRRALVSGFFMQVAKKSANGKNYVTMKDNQVVSLHPSCGLSVTPEWVVYN 675
Query: 550 EFVLTTK 570
EFVLTTK
Sbjct: 676 EFVLTTK 682
>sp|P53131|PRP43_YEAST Pre-mRNA splicing factor RNA helicase PRP43 (Helicase JA1)
Length = 767
Score = 113 bits (282), Expect = 4e-25
Identities = 55/97 (56%), Positives = 69/97 (71%), Gaps = 4/97 (4%)
Frame = +2
Query: 2 NLTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEA 181
NLT LG + ++FPLDP LA M+I S +F CS EIL+I AMLSVP F+RPT+ + AD+A
Sbjct: 509 NLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRADDA 568
Query: 182 KMGFAHIDGDHLTMLNVYHAFKQNREDS----QWCYD 280
K FAH DGDH+T+LNVYHAFK + +WC D
Sbjct: 569 KNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRD 605
Score = 73.6 bits (179), Expect = 3e-13
Identities = 39/57 (68%), Positives = 45/57 (78%), Gaps = 2/57 (3%)
Frame = +1
Query: 406 QKALVSGFFMQVAHLERSGH--YLTVKDNQIVHLHPSTCLDHKPEWVLYNEFVLTTK 570
+KAL SGFFMQVA +RSG Y+TVKDNQ V +HPST L H EWV+YNEFVLT+K
Sbjct: 649 RKALASGFFMQVAK-KRSGAKGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTSK 704
Score = 30.0 bits (66), Expect = 4.3
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +3
Query: 306 AADNVRIQLSRIMDRFNL 359
AADN+R QL R+M+R+NL
Sbjct: 615 AADNIRSQLERLMNRYNL 632
>sp|O42643|DHX8_SCHPO Putative pre-mRNA splicing factor ATP-dependent RNA helicase
C10F6.02c
Length = 1168
Score = 101 bits (251), Expect = 2e-21
Identities = 47/92 (51%), Positives = 63/92 (68%)
Frame = +2
Query: 5 LTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEAK 184
LT LG MA+FP++PQL+K++I S + CS E+LSI AMLSVP + RP E + AD +
Sbjct: 919 LTPLGRKMADFPMEPQLSKVLITSVELGCSEEMLSIIAMLSVPNIWSRpreKQQEADRQR 978
Query: 185 MGFAHIDGDHLTMLNVYHAFKQNREDSQWCYD 280
FA+ + DHLT+LNVY +K NR WCY+
Sbjct: 979 AQFANPESDHLTLLNVYTTWKMNRCSDNWCYE 1010
Score = 43.9 bits (102), Expect = 3e-04
Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 2/56 (3%)
Frame = +1
Query: 409 KALVSGFFMQVAHLE-RSGHYLTVKDNQIVHLHPSTCLDHKP-EWVLYNEFVLTTK 570
+AL SG+F VA + G Y T+ +N V++HPS L K EWV+Y+E + T+K
Sbjct: 1051 RALCSGYFTNVAKRDSHEGCYKTIVENAPVYMHPSGVLFGKAAEWVIYHELIQTSK 1106
>sp|Q14562|DHX8_HUMAN ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8)
Length = 1220
Score = 97.1 bits (240), Expect = 3e-20
Identities = 45/92 (48%), Positives = 61/92 (66%)
Frame = +2
Query: 5 LTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEAK 184
LT LG MAEFPL+P L KM+I S CS E+L+I +MLSV F RP + + AD+ K
Sbjct: 973 LTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKK 1032
Query: 185 MGFAHIDGDHLTMLNVYHAFKQNREDSQWCYD 280
F +GDHLT+L VY+++K N+ + WCY+
Sbjct: 1033 AKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYE 1064
Score = 59.7 bits (143), Expect = 5e-09
Identities = 29/66 (43%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +1
Query: 376 ISARRILCKHQKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCL-DHKPEWVLYNE 552
+S + + QKA+ SGFF A + Y T+ D Q+V++HPS+ L + +PEWV+Y+E
Sbjct: 1094 VSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHE 1153
Query: 553 FVLTTK 570
VLTTK
Sbjct: 1154 LVLTTK 1159
>sp|Q92620|PRP16_HUMAN Pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
(ATP-dependent RNA helicase DHX38) (DEAH-box protein 38)
Length = 1227
Score = 96.7 bits (239), Expect = 4e-20
Identities = 47/92 (51%), Positives = 58/92 (63%)
Frame = +2
Query: 5 LTDLGSMMAEFPLDPQLAKMVIASCDFNCSNEILSITAMLSVPQCFVRPTEARTAADEAK 184
LT G +M EFPLDP L+KM+I SCD CS+EIL I +MLSVP F RP +D+ +
Sbjct: 939 LTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIR 998
Query: 185 MGFAHIDGDHLTMLNVYHAFKQNREDSQWCYD 280
FA + DHLT LNVY +K N + WC D
Sbjct: 999 EKFAVPESDHLTYLNVYLQWKNNNYSTIWCND 1030
Score = 45.1 bits (105), Expect = 1e-04
Identities = 19/58 (32%), Positives = 36/58 (62%), Gaps = 3/58 (5%)
Frame = +1
Query: 406 QKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCL---DHKPEWVLYNEFVLTTK 570
+K + + +F Q A L+ G Y+ ++ HLHP++ L + P++++Y+E V+TTK
Sbjct: 1070 RKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTK 1127
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,481,810
Number of Sequences: 369166
Number of extensions: 1457169
Number of successful extensions: 3993
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3969
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)