Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_D13
(443 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35880|TRA1_LACHE Transposase for insertion sequence ele... 33 0.37
sp|Q15506|SP17_HUMAN Sperm surface protein Sp17 (Sperm auto... 32 0.82
sp|Q95230|SP17_PAPHA Sperm surface protein Sp17 (Sperm auto... 32 0.82
sp|O19021|SP17_MACFA Sperm surface protein Sp17 (Sperm auto... 32 0.82
sp|P39098|MA1A2_MOUSE Mannosyl-oligosaccharide 1,2-alpha-ma... 31 1.4
sp|Q72UA7|RPOC_LEPIC DNA-directed RNA polymerase beta' chai... 31 1.4
sp|P20627|NIFB_ANASP FeMo cofactor biosynthesis protein nifB 30 2.4
sp|O60476|MA1A2_HUMAN Mannosyl-oligosaccharide 1,2-alpha-ma... 30 2.4
sp|P12647|VGLG_SIGMA Spike glycoprotein precursor 29 4.0
sp|Q65QY0|RPOA_MANSM DNA-directed RNA polymerase alpha chai... 29 4.0
>sp|P35880|TRA1_LACHE Transposase for insertion sequence element IS1201
Length = 369
Score = 32.7 bits (73), Expect = 0.37
Identities = 23/71 (32%), Positives = 30/71 (42%), Gaps = 22/71 (30%)
Frame = +2
Query: 65 FQACWDPSVRECFRNTLNCLEP--LFEYNWPKD--------------------RVKPKKE 178
F A WD S RN L +EP L YN+PK +VKPK E
Sbjct: 292 FYAKWDKSYNHVIRN-LKDIEPDLLVFYNYPKQIRASIYSTNMIESFNNVIKRKVKPKAE 350
Query: 179 FPENEEMHTYL 211
FP + + T++
Sbjct: 351 FPTEQSLDTFI 361
>sp|Q15506|SP17_HUMAN Sperm surface protein Sp17 (Sperm autoantigenic protein 17) (Sperm
protein 17) (Sp17-1)
Length = 151
Score = 31.6 bits (70), Expect = 0.82
Identities = 29/105 (27%), Positives = 44/105 (41%), Gaps = 18/105 (17%)
Frame = +2
Query: 8 TREFLKQNQDNLTPVGASFFQ-------------ACWDPSVRECFRNTLNCLEPLFEYNW 148
TRE L++ DN+ A++F+ A W V + F N FE
Sbjct: 24 TREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYN-----NHAFEEQE 78
Query: 149 PKDRVKPKKE-----FPENEEMHTYLDKYRDVKEIREEVLKERLK 268
P ++ PK+E E E T LD + KE +EEV +++
Sbjct: 79 PPEKSDPKQEESQISGKEEETSVTILDSSEEDKE-KEEVAAVKIQ 122
>sp|Q95230|SP17_PAPHA Sperm surface protein Sp17 (Sperm autoantigenic protein 17)
Length = 163
Score = 31.6 bits (70), Expect = 0.82
Identities = 29/105 (27%), Positives = 44/105 (41%), Gaps = 18/105 (17%)
Frame = +2
Query: 8 TREFLKQNQDNLTPVGASFFQ-------------ACWDPSVRECFRNTLNCLEPLFEYNW 148
TRE L++ DN+ A++F+ A W V + F N FE
Sbjct: 24 TREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYN-----NHAFEEQE 78
Query: 149 PKDRVKPKKE-----FPENEEMHTYLDKYRDVKEIREEVLKERLK 268
P ++ PK+E E E T LD + KE +EEV +++
Sbjct: 79 PPEKSDPKQEESQVSGKEEETSVTILDSSEEDKE-KEEVAAVKIQ 122
>sp|O19021|SP17_MACFA Sperm surface protein Sp17 (Sperm autoantigenic protein 17)
Length = 151
Score = 31.6 bits (70), Expect = 0.82
Identities = 29/105 (27%), Positives = 44/105 (41%), Gaps = 18/105 (17%)
Frame = +2
Query: 8 TREFLKQNQDNLTPVGASFFQ-------------ACWDPSVRECFRNTLNCLEPLFEYNW 148
TRE L++ DN+ A++F+ A W V + F N FE
Sbjct: 24 TREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYN-----NHAFEEQE 78
Query: 149 PKDRVKPKKE-----FPENEEMHTYLDKYRDVKEIREEVLKERLK 268
P ++ PK+E E E T LD + KE +EEV +++
Sbjct: 79 PPEKSDPKQEESQIPGKEEEASVTILDSSEEDKE-KEEVAAVKIQ 122
>sp|P39098|MA1A2_MOUSE Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (Processing
alpha-1,2-mannosidase IB) (Alpha-1,2-mannosidase IB)
(Mannosidase alpha class 1A member 2)
Length = 641
Score = 30.8 bits (68), Expect = 1.4
Identities = 24/75 (32%), Positives = 36/75 (48%), Gaps = 8/75 (10%)
Frame = +2
Query: 191 EEMHTYLDKYRDVKEIREEVLKERLKTVDPFVVKP-RLKFPLAVPD-------DPDEIGF 346
EE L K R+ EIR E+ E+ K K R+ P+ VP DP+++
Sbjct: 120 EEAKEKLRKSRE--EIRAEIQTEKNKVAQAMKTKETRVLPPVPVPQRVGVSGGDPEDMEI 177
Query: 347 VKERRKDKELRRNYW 391
K+R K KE+ ++ W
Sbjct: 178 KKKRDKIKEMMKHAW 192
>sp|Q72UA7|RPOC_LEPIC DNA-directed RNA polymerase beta' chain (RNAP beta' subunit)
(Transcriptase beta' chain) (RNA polymerase beta'
subunit)
sp|Q8F0S3|RPOC_LEPIN DNA-directed RNA polymerase beta' chain (RNAP beta' subunit)
(Transcriptase beta' chain) (RNA polymerase beta'
subunit)
Length = 1404
Score = 30.8 bits (68), Expect = 1.4
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +2
Query: 149 PKDRVKPKKEFPENEEMHTYLDKYRD--VKEIREEVLKERLKTVD 277
P D + + E + EE H YLD+Y D V I + +KE L +D
Sbjct: 139 PADSGRNRGELIDEEEYHGYLDEYGDKFVAGIGADAIKELLARID 183
>sp|P20627|NIFB_ANASP FeMo cofactor biosynthesis protein nifB
Length = 475
Score = 30.0 bits (66), Expect = 2.4
Identities = 26/125 (20%), Positives = 54/125 (43%), Gaps = 23/125 (18%)
Frame = +2
Query: 92 RECFRNTLNCLEPLFEYNWPKDR---VKPKKEFPENEEMHTYLDKYR-DVKEIREEVLKE 259
R+C + + L + KD+ + P+ +F + +E+H ++K+R ++K +E+VL
Sbjct: 298 RQCRADAVGLLGEDRSQEFTKDKFLEMAPEYDFDKRQEVHEGIEKFRVELKVAKEKVLAG 357
Query: 260 RLKTVDPFVVKPRLKFPLAVPD-------------------DPDEIGFVKERRKDKELRR 382
+ KT + P++ +A D E+ FV R+ D +
Sbjct: 358 KEKTAN----NPKILVAIATKGGGLVNQHFGHAKEFQVYEVDGSEVSFVSHRKVDHYCQG 413
Query: 383 NYWKD 397
Y ++
Sbjct: 414 GYGEE 418
>sp|O60476|MA1A2_HUMAN Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (Processing
alpha-1,2-mannosidase IB) (Alpha-1,2-mannosidase IB)
(Mannosidase alpha class 1A member 2)
Length = 641
Score = 30.0 bits (66), Expect = 2.4
Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 8/75 (10%)
Frame = +2
Query: 191 EEMHTYLDKYRDVKEIREEVLKERLKTVDPFVVKPRLKFP-LAVPD-------DPDEIGF 346
EE L K R+ EIR E+ E+ K V +K P + +P+ DP++
Sbjct: 120 EEAKEKLRKSRE--EIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDI 177
Query: 347 VKERRKDKELRRNYW 391
++R K KE+ ++ W
Sbjct: 178 REKREKIKEMMKHAW 192
>sp|P12647|VGLG_SIGMA Spike glycoprotein precursor
Length = 526
Score = 29.3 bits (64), Expect = 4.0
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +3
Query: 57 LHSFKHAGIPLLENVSEILLTVWNRYLNTTGLKIG*NQK--KNFLKTKKCILILTNIVM 227
+H+ H + ++ N +E +T WNR +N+T L IG + F K LIL IV+
Sbjct: 432 VHTVSHPILHVISNHTEQSVTTWNRGVNSTNL-IGQATRSISGFYNDLKLYLILALIVV 489
>sp|Q65QY0|RPOA_MANSM DNA-directed RNA polymerase alpha chain (RNAP alpha subunit)
(Transcriptase alpha chain) (RNA polymerase alpha
subunit)
Length = 329
Score = 29.3 bits (64), Expect = 4.0
Identities = 23/100 (23%), Positives = 42/100 (42%), Gaps = 12/100 (12%)
Frame = +2
Query: 32 QDNLTPVGASFFQACWDPSVRECF---------RNTLNCLEPLFEYNW---PKDRVKPKK 175
QD P+G AC+ P R + R L+ L E N P++ ++
Sbjct: 162 QDEERPIGRLLVDACYSPVDRIAYNVEAARVEQRTDLDKLVIELETNGAIDPEEAIRRAA 221
Query: 176 EFPENEEMHTYLDKYRDVKEIREEVLKERLKTVDPFVVKP 295
+ LD + D++++R+ +KE DP +++P
Sbjct: 222 TI-----LAEQLDAFVDLRDVRQPEVKEEKPEFDPILLRP 256
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,940,744
Number of Sequences: 369166
Number of extensions: 947660
Number of successful extensions: 3644
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3641
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2344429560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)