Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_D11
(745 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligas... 223 4e-58
sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligas... 223 4e-58
sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligas... 188 1e-47
sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligas... 187 3e-47
sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligas... 130 3e-30
sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligas... 124 3e-28
sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligas... 122 1e-27
sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligas... 120 3e-27
sp|P53556|BIOF_BACSU 8-amino-7-oxononanoate synthase (AONS)... 114 2e-25
sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligas... 114 3e-25
>sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 416
Score = 223 bits (568), Expect = 4e-58
Identities = 113/204 (55%), Positives = 149/204 (73%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
APL++I ++ +G L+FVDECH+TGF+GPTGRG++E + V IINSTLGKA+GG
Sbjct: 209 APLQDICRLAAQYGALVFVDECHATGFLGPTGRGTDELLGVMDQ-VTIINSTLGKALGGA 267
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
GGYT G L++ LRQ+SRPYLFSN+LPP+VV + +A S + T R
Sbjct: 268 SGGYTTGPEPLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQS-MAAKTRR 326
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
FR++M GF + G HPICPV+LGDA+L+S+ A+ MLKKGI+VIGFSYPVVPK KARI
Sbjct: 327 FRSKMEAAGFTVSG-ADHPICPVMLGDARLSSQMADDMLKKGIFVIGFSYPVVPKGKARI 385
Query: 542 RVQISAVHTTQQINKAIDAFRQVG 613
RVQISAVH+ + I++ ++AF +VG
Sbjct: 386 RVQISAVHSEEDIDRCVEAFVEVG 409
>sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 419
Score = 223 bits (568), Expect = 4e-58
Identities = 114/204 (55%), Positives = 149/204 (73%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
APL+EI ++ +G L+F+DECH+TGF+GPTGRG++E + V IINSTLGKA+GG
Sbjct: 212 APLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQ-VTIINSTLGKALGGA 270
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
GGYT G L++ LRQ++RPYLFSN+LPP+VV + +A S + T R
Sbjct: 271 SGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQS-MAAKTQR 329
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
FR++M GF I G SHPICPV+LGDA+LAS A+ MLK+GI+VIGFSYPVVPK KARI
Sbjct: 330 FRSKMEAAGFTISG-ASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARI 388
Query: 542 RVQISAVHTTQQINKAIDAFRQVG 613
RVQISAVH+ + I++ ++AF +VG
Sbjct: 389 RVQISAVHSEEDIDRCVEAFVEVG 412
>sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 188 bits (478), Expect = 1e-47
Identities = 99/210 (47%), Positives = 136/210 (64%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L+ + +++ + L+ VD+ H+ GFVG GRGS E+ D+ VDII TLGKA+GG
Sbjct: 191 ANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDV-MGRVDIITGTLGKALGGA 249
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
GGYT ++ LRQ+SRPYLFSN+L P++VAASI+ ++ N +
Sbjct: 250 SGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLEMVEAGAELR-DRLWANARQ 308
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
FR +M+ GF + G H I PV+LGDA +A +FA + K+GIYV GF YPVVPK +ARI
Sbjct: 309 FREQMSAAGFTLAG-ADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVVPKGQARI 367
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R Q+SA HT +QI +A+DAF ++G L VI
Sbjct: 368 RTQMSAAHTPEQITRAVDAFTRIGKQLGVI 397
>sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB79|KBL_SHIFL 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB78|KBL_ECO57 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 187 bits (474), Expect = 3e-47
Identities = 98/210 (46%), Positives = 136/210 (64%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L+ + +++ + L+ VD+ H+ GFVG GRGS E+ D+ VDII TLGKA+GG
Sbjct: 191 ANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDV-MGRVDIITGTLGKALGGA 249
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
GGYT ++ LRQ+SRPYLFSN+L P++VAASI+ ++ N +
Sbjct: 250 SGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLEMVEAGSELR-DRLWANARQ 308
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
FR +M+ GF + G H I PV+LGDA +A +FA + K+GIYV GF YPVVPK +ARI
Sbjct: 309 FREQMSAAGFTLAG-ADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVVPKGQARI 367
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R Q+SA HT +QI +A++AF ++G L VI
Sbjct: 368 RTQMSAAHTPEQITRAVEAFTRIGKQLGVI 397
>sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 392
Score = 130 bits (328), Expect = 3e-30
Identities = 72/210 (34%), Positives = 121/210 (57%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
APL +I+ ++E + + VD+ H++G +G GRG+ + L V I TL KA+G
Sbjct: 187 APLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGLDGR-VHIQVGTLSKAIG-V 244
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
+GGY GS LI+ LR K RP+LFS + PP+V AA ++A ++ ENT
Sbjct: 245 LGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEAIDVLLEEPEHM-ERLWENTAY 303
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
F+ + +G + + PI P+L+GD +A +F++ +L +G++ +P V K KARI
Sbjct: 304 FKAMLVKMGLTLTKSET-PILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKARI 362
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R I+A HT ++++A+D + +L+++
Sbjct: 363 RTIITAEHTKDELDQALDVIEKTAKELQLL 392
>sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|Q8NXY3|KBL_STAAW 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60121|KBL_STAAM 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60120|KBL_STAAN 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 124 bits (311), Expect = 3e-28
Identities = 71/210 (33%), Positives = 119/210 (56%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L EI+ ++E+ G L +VD+ H +G +G G G+ + + L Q +D TL KA+G
Sbjct: 191 AKLPEIVEIAEEFGLLTYVDDAHGSGVMGK-GAGTVKHFGL-QDKIDFQIGTLSKAIG-V 247
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
+GGY G+ LI+ L+ +SRP+LFS +L P A +A K+ +N
Sbjct: 248 VGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEAVKKLMDSTELH-DKLWDNAQY 306
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
+ ++ LG++ G+ PI PV++GD K EF++ + +G+YV +P VP+ R+
Sbjct: 307 LKNGLSKLGYDT-GESETPITPVIIGDEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRV 365
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R +A HT +++AI A+ +VG ++K+I
Sbjct: 366 RNMPTAAHTKDMLDEAIAAYEKVGKEMKLI 395
>sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 122 bits (305), Expect = 1e-27
Identities = 71/210 (33%), Positives = 119/210 (56%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L EI+ ++E+ + +VD+ H +G G G G+ + + L Q VD+ TL KA+G
Sbjct: 192 AKLPEIVDIAEEFDLITYVDDAHGSGVTGD-GAGTVKHFGL-QDKVDMQMGTLSKAIG-V 248
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
+GGY G SLI+ L+ +SRP+LFS + P+ AS +A K+ EN
Sbjct: 249 IGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRAIEMLMESTELN-EKLWENGDY 307
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
+ + LGF+I G+ PI P ++GD K A EF++ + ++G+Y +P VP+ R+
Sbjct: 308 LKQGLKELGFDI-GESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVPRGTGRV 366
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R +A HT + ++ AI ++++G ++K+I
Sbjct: 367 RNMPTAAHTKEMLDDAIAIYKKIGQEMKLI 396
>sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 120 bits (302), Expect = 3e-27
Identities = 69/210 (32%), Positives = 118/210 (56%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L EI+ ++E+ G L +VD+ H +G +G G G+ + + L Q +D TL KA+G
Sbjct: 191 AKLPEIVEIAEEFGLLTYVDDAHGSGVMGK-GAGTVKHFGL-QDKIDFQIGTLSKAIG-V 247
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
+GGY G+ LI+ L+ +SRP+LFS +L P A +A K+ +N
Sbjct: 248 VGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEAVKKLMASTELH-DKLWDNAQY 306
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
+ ++ LG++ G+ PI PV++G+ K EF++ + +G+YV +P VP+ R+
Sbjct: 307 LKNGLSKLGYDT-GESETPITPVIIGEEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRV 365
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R +A HT +++AI A+ +VG ++ +I
Sbjct: 366 RNMPTAAHTKDMLDEAIAAYEKVGKEMNLI 395
>sp|P53556|BIOF_BACSU 8-amino-7-oxononanoate synthase (AONS) (8-amino-7-ketopelargonate
synthase) (7-keto-8-amino-pelargonic acid synthetase)
(7-KAP synthetase) (L-alanine--pimelyl CoA ligase)
Length = 389
Score = 114 bits (286), Expect = 2e-25
Identities = 66/210 (31%), Positives = 114/210 (54%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
APL +I+++++ + + VD+ H+TG +G +G+G+ E++ + C DI+ TL KA+G
Sbjct: 186 APLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGV---CPDIVIGTLSKAVGAE 242
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
GG+ GS I+ L +R ++F +PP+ AA+ +A +
Sbjct: 243 -GGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKR-QLLFSYISM 300
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
RT + N+G+ + GD + PI PV++GDA FAE + KGIY P V ++RI
Sbjct: 301 IRTSLKNMGYVVKGDHT-PIIPVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRI 359
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R+ I++ H+ I+ + F +G +L +I
Sbjct: 360 RITITSDHSMGDIDHLLQTFHSIGKELHII 389
>sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 114 bits (285), Expect = 3e-25
Identities = 68/210 (32%), Positives = 116/210 (55%)
Frame = +2
Query: 2 APLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAMGGT 181
A L EI+ ++E+ + +VD+ H +G +G G G+ + + L VD TL KA+G
Sbjct: 192 AKLPEIVEIAEELDLMTYVDDAHGSGVLGK-GAGTVKHFGLSDK-VDFQIGTLSKAIG-V 248
Query: 182 MGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIRENTHR 361
+GGY G +LI+ L+ +SRP+LFS L P+ AA +++ ++ EN
Sbjct: 249 IGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELH-DRLWENGRY 307
Query: 362 FRTEMTNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARI 541
+ + LGFNI G+ PI P ++GD L EF++ + ++G+Y +P V K R+
Sbjct: 308 LKQGLKELGFNI-GESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRV 366
Query: 542 RVQISAVHTTQQINKAIDAFRQVGTDLKVI 631
R +A HT + +++AI + +VG ++ +I
Sbjct: 367 RNMPTAAHTKEMLDEAILKYEKVGKEMGII 396
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,801,082
Number of Sequences: 369166
Number of extensions: 1737214
Number of successful extensions: 4126
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3903
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3995
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6728100400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)