Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_C23
(741 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q15036|SNX17_HUMAN Sorting nexin-17 64 6e-10
sp|P54939|TLN1_CHICK Talin-1 31 3.0
sp|P25386|USO1_YEAST Intracellular protein transport protei... 31 3.0
sp|Q9ZD41|Y506_RICPR Hypothetical protein RP506 31 4.0
sp|Q89AP1|RRF_BUCBP Ribosome recycling factor (Ribosome rel... 30 6.8
sp|Q8WUM0|NU133_HUMAN Nuclear pore complex protein Nup133 (... 30 6.8
sp|Q06190|2ACA_HUMAN Serine/threonine protein phosphatase 2... 30 8.8
sp|Q66GS9|CEP4_HUMAN Centrosomal protein 4 (Centrosomal pro... 30 8.8
sp|Q9UUL4|MOK12_SCHPO Cell wall alpha-1,3-glucan synthase m... 30 8.8
>sp|Q15036|SNX17_HUMAN Sorting nexin-17
Length = 470
Score = 63.5 bits (153), Expect = 6e-10
Identities = 46/168 (27%), Positives = 91/168 (54%), Gaps = 2/168 (1%)
Frame = +1
Query: 1 EIVNGWISIPDEARMNRIQNLIERKARKETLQLARTLSYYGYMQLHGTSVCNRPLPNTMA 180
+I GWI + E ++++L E+ ++KE L+LA+TL +YGY++ V + P +
Sbjct: 231 DIERGWILVTKEQH-RQLKSLQEKVSKKEFLRLAQTLRHYGYLRF-DACVADFPEKDCPV 288
Query: 181 IVSTGNFEIVFRDEHDDHVLMK--YYLTRIKAWKISTALKEPLNDDINQSVNQSQALSPT 354
+VS GN E+ + L + + +TR++ W++++++ P + + + +
Sbjct: 289 VVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGE-VRLE 347
Query: 355 LTFEYLDIETKKLDEVVISNNQTDQIILLSMSLKNMAEEYVCYKSSNS 498
L FEYL + +L V I+ + Q I++S+ L++M +E + KS S
Sbjct: 348 LAFEYL-MSKDRLQWVTIT---SPQAIMMSICLQSMVDELMVKKSGGS 391
>sp|P54939|TLN1_CHICK Talin-1
Length = 2541
Score = 31.2 bits (69), Expect = 3.0
Identities = 24/79 (30%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
Frame = +1
Query: 58 NLIERKARKETLQLARTLSYYG---YMQLHGTSVCNRPLPNTMAIVSTGNFEIVFR-DEH 225
N+ E +A+ ++LAR+L YG ++ N+ +P + I E V R DE
Sbjct: 288 NMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITK----ECVMRVDEK 343
Query: 226 DDHVLMKYYLTRIKAWKIS 282
V+ ++ LT IK W S
Sbjct: 344 TKEVIQEWSLTNIKRWAAS 362
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 31.2 bits (69), Expect = 3.0
Identities = 30/117 (25%), Positives = 51/117 (43%), Gaps = 12/117 (10%)
Frame = +1
Query: 196 NFEIVFRDEHDDHVLMKYYLTRI-------KAWKISTAL-----KEPLNDDINQSVNQSQ 339
N + + E D+ L K Y + ++I TAL +EP+N + V + Q
Sbjct: 677 NEDSILTPELDETGLPKVYFSTYFIQLFNENIYRIRTALSHDPDEEPINKISFEEVEKLQ 736
Query: 340 ALSPTLTFEYLDIETKKLDEVVISNNQTDQIILLSMSLKNMAEEYVCYKSSNSSLSD 510
L E ++T+ N T+++I L+ K + E+Y SS+SSL +
Sbjct: 737 RQCTKLKGEITSLQTETEST---HENLTEKLIALTNEHKELDEKYQILNSSHSSLKE 790
>sp|Q9ZD41|Y506_RICPR Hypothetical protein RP506
Length = 201
Score = 30.8 bits (68), Expect = 4.0
Identities = 37/118 (31%), Positives = 54/118 (45%), Gaps = 17/118 (14%)
Frame = +1
Query: 262 IKAWKIST--ALKEPLN----DDINQ--SVNQSQALSPTLTFEYLDIETKKLD------- 396
+ A+KI T A+KE N D IN ++NQ QAL T +LD E+ LD
Sbjct: 77 LNAYKIKTERAIKESGNKYKLDIINAIYNINQDQALIITAKEGFLDQESNILDLKNDIKL 136
Query: 397 --EVVISNNQTDQIILLSMSLKNMAEEYVCYKSSNSSLSDVENGEKMMNTSKFTSNDS 564
+ +I N +I L++ ++ + + YK+S S SD N N F N S
Sbjct: 137 FFDEIIFNTNNARINLVNQNIYGNSSAKLLYKNS-SITSDSFNTMDENNIIIFKGNVS 193
>sp|Q89AP1|RRF_BUCBP Ribosome recycling factor (Ribosome releasing factor) (RRF)
Length = 183
Score = 30.0 bits (66), Expect = 6.8
Identities = 16/55 (29%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = +1
Query: 319 QSVNQSQALSPTLTFEYLD--IETKKLDEVVISNNQTDQIILLSMSLKNMAEEYV 477
+S S ++ T+ +YL I+ KKL +++ N T +I L +KN E+ +
Sbjct: 27 RSSRASPSILDTILIDYLGQKIQLKKLSNIIVENVNTLRITLFDPKIKNNVEKAI 81
>sp|Q8WUM0|NU133_HUMAN Nuclear pore complex protein Nup133 (Nucleoporin Nup133) (133 kDa
nucleoporin)
Length = 1156
Score = 30.0 bits (66), Expect = 6.8
Identities = 29/113 (25%), Positives = 48/113 (42%), Gaps = 3/113 (2%)
Frame = +1
Query: 268 AWKISTALKEPLNDDINQSVNQSQALSPTLTFEYLDIETKKLDEVVI---SNNQTDQIIL 438
+W I+ ALKE + D I S + +A+ + YLD + + D +VI + + D L
Sbjct: 309 SWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDFK-QNCDGLVILAAAWHSADNPCL 367
Query: 439 LSMSLKNMAEEYVCYKSSNSSLSDVENGEKMMNTSKFTSNDSYKPAIMCSLYI 597
+ SL + + + +SD E F S D I+C L +
Sbjct: 368 IYYSL-------ITIEDNGCQMSDAVTVEVTQYNPPFQSED----LILCQLTV 409
>sp|Q06190|2ACA_HUMAN Serine/threonine protein phosphatase 2A, 72/130 kDa regulatory
subunit B (PP2A, subunit B, B''-PR72/PR130) (PP2A,
subunit B, B72/B130 isoforms) (PP2A, subunit B,
PR72/PR130 isoforms) (PP2A, subunit B, R3 isoform)
Length = 1150
Score = 29.6 bits (65), Expect = 8.8
Identities = 35/131 (26%), Positives = 58/131 (44%), Gaps = 21/131 (16%)
Frame = +1
Query: 274 KISTALKEPLNDDINQSV--NQSQALSPTLTFEYLDIETKK-------------LDEVVI 408
K+ T+L+EPL N + + SQ TL +D+E K L ++
Sbjct: 522 KMETSLREPLAKGKNSNFLNSHSQLTGQTL----VDLEPKSKVSSPIEKVSPSCLTRIIE 577
Query: 409 SNN----QTDQIILLSM--SLKNMAEEYVCYKSSNSSLSDVENGEKMMNTSKFTSNDSYK 570
+N + D+ +LL + S+++ A+E V KSS SLS + E M + + S
Sbjct: 578 TNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLS--QEKEMMQILQETLTTSSQA 635
Query: 571 PAIMCSLYIGD 603
+C +GD
Sbjct: 636 NLSVCRSPVGD 646
>sp|Q66GS9|CEP4_HUMAN Centrosomal protein 4 (Centrosomal protein of 135 kDa) (Cep135
protein)
Length = 1140
Score = 29.6 bits (65), Expect = 8.8
Identities = 27/138 (19%), Positives = 58/138 (42%), Gaps = 20/138 (14%)
Frame = +1
Query: 154 NRPLPNTMAIVSTGNFEI------VFRDEHDDHVLMKYYLTRIKAWKISTALKEPLNDDI 315
NR L + +A ++ N EI +++ + + Y+T + W+ A KE N D+
Sbjct: 820 NRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDL 879
Query: 316 --------------NQSVNQSQALSPTLTFEYLDIETKKLDEVVISNNQTDQIILLSMSL 453
+Q++ S ++ E L I+T++ + +++ LL +
Sbjct: 880 LDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTER-------RHLRERVELLEKEI 932
Query: 454 KNMAEEYVCYKSSNSSLS 507
+ + Y+S SS++
Sbjct: 933 QEHINAHHAYESQISSMA 950
>sp|Q9UUL4|MOK12_SCHPO Cell wall alpha-1,3-glucan synthase mok12
Length = 2352
Score = 29.6 bits (65), Expect = 8.8
Identities = 26/111 (23%), Positives = 50/111 (45%), Gaps = 3/111 (2%)
Frame = +1
Query: 241 MKYYLTRIKAWKISTALKEPLNDDINQSVNQSQ---ALSPTLTFEYLDIETKKLDEVVIS 411
+K+ KA++ + L + +S+N+ Q A SP ++ E L EV
Sbjct: 1651 LKHASPTFKAYQFVVRICHYLTSKV-KSINKDQVFNAFSPDVS------ERPPLFEVASC 1703
Query: 412 NNQTDQIILLSMSLKNMAEEYVCYKSSNSSLSDVENGEKMMNTSKFTSNDS 564
+N+TD + L+ L E + +K NSS ++ +++ + SN +
Sbjct: 1704 SNETDSVEKLTPELSVSPESDMHFKDGNSSKLEIVESKEIYASDSDISNST 1754
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,911,608
Number of Sequences: 369166
Number of extensions: 1831196
Number of successful extensions: 4451
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4295
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4447
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6679696800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)