Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_C11
(635 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O61998|HSP90_BRUPA Heat shock protein 90 37 0.032
sp|P12861|HSP83_TRYBB Heat shock protein 83 37 0.042
sp|Q61136|PRP4B_MOUSE Serine/threonine-protein kinase PRP4 ... 35 0.16
sp|Q12498|PRM4_YEAST Pheromone-regulated membrane protein 4 33 0.80
sp|Q7VR84|TOLB_BLOFL Protein tolB precursor 32 1.8
sp|O51353|RL1_BORBU 50S ribosomal protein L1 31 2.3
sp|Q9AJ83|SCA4_RICAF Antigenic heat-stable 120 kDa protein ... 31 2.3
sp|P17078|RL35_RAT 60S ribosomal protein L35 31 3.0
sp|P22793|TRHY_SHEEP Trichohyalin 31 3.0
sp|P40341|RCA1_YEAST Mitochondrial respiratory chain comple... 31 3.0
>sp|O61998|HSP90_BRUPA Heat shock protein 90
Length = 717
Score = 37.4 bits (85), Expect = 0.032
Identities = 27/95 (28%), Positives = 49/95 (51%), Gaps = 7/95 (7%)
Frame = +2
Query: 104 DPTLKKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHE----YKIKLELYKNKEKTITDD- 268
DP L + ++TL + +Q D L+ + +E +KKH Y IKL + K ++K ++DD
Sbjct: 167 DPELTRGTKITL--YIKEDQTDYLEERRIKEIVKKHSQFIGYPIKLTVEKERDKEVSDDE 224
Query: 269 --NQVPNAFKKAKQDILGSSGDLQQNIGKDSPSKK 367
+ + K+ K+ + G+ ++ KD KK
Sbjct: 225 AEEEKKDEDKEKKEGEIEDVGEDEEEDKKDKDKKK 259
>sp|P12861|HSP83_TRYBB Heat shock protein 83
Length = 703
Score = 37.0 bits (84), Expect = 0.042
Identities = 26/93 (27%), Positives = 45/93 (48%), Gaps = 4/93 (4%)
Frame = +2
Query: 101 PDPTLKKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHE----YKIKLELYKNKEKTITDD 268
PD LK+ + L D +Y + +R D +KKH Y I+L + EK +TD+
Sbjct: 161 PDCDLKRGTRIVLHLKEDQQEYLEERRLKD--LIKKHSEFIGYDIELMVENTTEKEVTDE 218
Query: 269 NQVPNAFKKAKQDILGSSGDLQQNIGKDSPSKK 367
++ A KKA++ +++ + D+ KK
Sbjct: 219 DEDEEAAKKAEEGEEPKVEEVKDGVDADAKKKK 251
>sp|Q61136|PRP4B_MOUSE Serine/threonine-protein kinase PRP4 homolog (PRP4 pre-mRNA
processing factor 4 homolog) (Pre-mRNA protein kinase)
Length = 1007
Score = 35.0 bits (79), Expect = 0.16
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +2
Query: 104 DPTLKKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHEYKIKLELYKNKEKTITDDNQVPN 283
D + K + KK +++ K +E+R KKH++K K + +K KE D + +
Sbjct: 35 DQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDRDKKHKHKHKHKKHKRKEVIEASDKEGLS 94
Query: 284 AFKKAKQDILGSSGDLQQ 337
K+ K D L DL++
Sbjct: 95 PAKRTKLDDLALLEDLEK 112
>sp|Q12498|PRM4_YEAST Pheromone-regulated membrane protein 4
Length = 284
Score = 32.7 bits (73), Expect = 0.80
Identities = 21/76 (27%), Positives = 41/76 (53%)
Frame = +2
Query: 143 KFYDGNQYDDLKRQLDEERMKKHEYKIKLELYKNKEKTITDDNQVPNAFKKAKQDILGSS 322
+FY+ + K+ +EE KK YK+ L K + ++++N++ F+K K++I
Sbjct: 43 EFYNSADPSENKKNSEEESEKKFVYKLP-NLLKTADSFLSNENEL--NFQKVKEEISNIQ 99
Query: 323 GDLQQNIGKDSPSKKA 370
+++ +I PS KA
Sbjct: 100 SEVEVDI--PEPSSKA 113
>sp|Q7VR84|TOLB_BLOFL Protein tolB precursor
Length = 460
Score = 31.6 bits (70), Expect = 1.8
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +1
Query: 22 SDQRGSVQIYR*NHSGNHSSRRIRILSGSNFK 117
SDQ G Q+Y+ N + N S RI L+GSN K
Sbjct: 312 SDQGGVPQVYKININNNRDSHRISWLNGSNQK 343
>sp|O51353|RL1_BORBU 50S ribosomal protein L1
Length = 226
Score = 31.2 bits (69), Expect = 2.3
Identities = 21/74 (28%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
Frame = +2
Query: 113 LKKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHEYKIKLELYKNKEK--TITDDNQVPNA 286
++ F++V KFY+ +D L E + K + I + + N +K T+ D +PN
Sbjct: 9 IEAFSKVDKNKFYN---IEDAILLLKEIKFAKFDETIDISINLNLKKNHTVRDTIVLPNQ 65
Query: 287 FKKAKQDILGSSGD 328
F K K+ ++ + GD
Sbjct: 66 FMKPKRILVFAKGD 79
>sp|Q9AJ83|SCA4_RICAF Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
(Protein PS 120)
Length = 1011
Score = 31.2 bits (69), Expect = 2.3
Identities = 22/81 (27%), Positives = 39/81 (48%), Gaps = 2/81 (2%)
Frame = +2
Query: 116 KKFNEVTLKKFYDGN--QYDDLKRQLDEERMKKHEYKIKLELYKNKEKTITDDNQVPNAF 289
+K + TL F+ GN + + + LD +KK I++ YKN T + + P F
Sbjct: 117 EKEKDKTLSTFF-GNPANREFIDKALDNPELKKKLESIEIAGYKNVHNTFSAASGYPGGF 175
Query: 290 KKAKQDILGSSGDLQQNIGKD 352
K + + S+ DL+ + K+
Sbjct: 176 KPVQWENHVSASDLRATVVKN 196
>sp|P17078|RL35_RAT 60S ribosomal protein L35
Length = 123
Score = 30.8 bits (68), Expect = 3.0
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 5/44 (11%)
Frame = +2
Query: 137 LKKFYDGNQYDDL-----KRQLDEERMKKHEYKIKLELYKNKEK 253
L+KFY G +Y L K + R+ KHE K+K + + KE+
Sbjct: 69 LRKFYKGKKYKPLDLRPKKTRAMRRRLTKHEEKLKTKKQQRKER 112
>sp|P22793|TRHY_SHEEP Trichohyalin
Length = 1549
Score = 30.8 bits (68), Expect = 3.0
Identities = 18/74 (24%), Positives = 40/74 (54%)
Frame = +2
Query: 116 KKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHEYKIKLELYKNKEKTITDDNQVPNAFKK 295
+K N + ++ D +++ RQL+E R+K+ E + E + +EK + + ++ ++
Sbjct: 104 RKGNPLQDRRREDQRRFEPQDRQLEERRLKRQELEELAEEEELREKQVRREQRL----QR 159
Query: 296 AKQDILGSSGDLQQ 337
+Q+ G +LQQ
Sbjct: 160 REQEEYGGEEELQQ 173
>sp|P40341|RCA1_YEAST Mitochondrial respiratory chain complexes assembly protein RCA1
(TAT-binding homolog 12)
Length = 825
Score = 30.8 bits (68), Expect = 3.0
Identities = 23/101 (22%), Positives = 46/101 (45%)
Frame = +2
Query: 113 LKKFNEVTLKKFYDGNQYDDLKRQLDEERMKKHEYKIKLELYKNKEKTITDDNQVPNAFK 292
++K+ E T N + + KR++DE + + +K E + +E+ + + N
Sbjct: 77 VEKYIEQTKNNTIPAN-WKEQKRKIDESIRRLEDAVLKQESNRIQEER--KEKEEENGPS 133
Query: 293 KAKQDILGSSGDLQQNIGKDSPSKKAPLPADGPVDSHSSPL 415
KAK + G + N ++ P P P P++ S+P+
Sbjct: 134 KAKSNRTKEQGYFEGNNSRNIPPPPPPPPPKPPLNDPSNPV 174
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,096,638
Number of Sequences: 369166
Number of extensions: 928130
Number of successful extensions: 3615
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3463
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3605
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5121172350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)