Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_B05
(733 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00816|HEX2_YEAST Protein HEX2 (Protein SRN1) (Protein R... 35 0.16
sp|P22620|ABRA_PLAFC 101 kDa malaria antigen (P101) (Acidic... 35 0.27
sp|P23745|ABRA_PLAFG 101 kDa malaria antigen (P101) (Acidic... 35 0.27
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 33 1.0
sp|P19269|AMY1_DEBOC Alpha-amylase 1 precursor (1,4-alpha-D... 32 1.3
sp|Q9TU19|NPHP1_CANFA Nephrocystin-1 32 1.3
sp|Q00496|BXE_CLOBO Botulinum neurotoxin type E precursor (... 32 1.7
sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2) 32 2.3
sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HS... 32 2.3
sp|P30995|BXE_CLOBU Botulinum neurotoxin type E precursor (... 32 2.3
>sp|Q00816|HEX2_YEAST Protein HEX2 (Protein SRN1) (Protein REG1)
Length = 1014
Score = 35.4 bits (80), Expect = 0.16
Identities = 25/78 (32%), Positives = 44/78 (56%), Gaps = 1/78 (1%)
Frame = -2
Query: 330 KSTYQEIRDQISIENIKWIGQNSIGLLSNIKESLSAKYKVEVGQNPSVEEVNQND-EDST 154
KS + + +D +I N +G+NS G +E L K + V N S + N++ E+ST
Sbjct: 351 KSQHSDKKDNSTIPNP--VGENSNGGGDKGEEDLHLKSALHVQNNRSTAQSNKSILENST 408
Query: 153 HDEEANLRDYLSTNDDHQ 100
+D +ANL L++ D+++
Sbjct: 409 NDRKANLDQNLNSPDNNR 426
>sp|P22620|ABRA_PLAFC 101 kDa malaria antigen (P101) (Acidic basic repeat antigen)
Length = 743
Score = 34.7 bits (78), Expect = 0.27
Identities = 34/155 (21%), Positives = 69/155 (44%), Gaps = 17/155 (10%)
Frame = -2
Query: 519 KKNSGDESKTESTVMQLAQNFSNKLRVSVRAMST---VCYNHMSSGN------------K 385
KKN+ +E K +Q + L+ ++ ++ + Y + S K
Sbjct: 107 KKNNAEEMKNLVNFLQSHKKLIKALKKNIESIQNKKHLIYKNKSYNPLLLSCVKKMNMLK 166
Query: 384 ETLDLMFNKTEHIYSTL--SKSTYQEIRDQISIENIKWIGQNSIGLLSNIKESLSAKYKV 211
E +D + K ++++ L K+TY + + I ++K G + + + + KY+
Sbjct: 167 ENVDYI-QKNQNLFKELMNQKATYSFVNTKKKIISLKSQGHKKETSQNQNENNDNQKYQE 225
Query: 210 EVGQNPSVEEVNQNDEDSTHDEEANLRDYLSTNDD 106
++ +E + ND++ T+DEE + D TNDD
Sbjct: 226 VNDEDDVNDEEDTNDDEDTNDEE-DTNDDEDTNDD 259
>sp|P23745|ABRA_PLAFG 101 kDa malaria antigen (P101) (Acidic basic repeat antigen)
Length = 600
Score = 34.7 bits (78), Expect = 0.27
Identities = 34/155 (21%), Positives = 69/155 (44%), Gaps = 17/155 (10%)
Frame = -2
Query: 519 KKNSGDESKTESTVMQLAQNFSNKLRVSVRAMST---VCYNHMSSGN------------K 385
KKN+ +E K +Q + L+ ++ ++ + Y + S K
Sbjct: 24 KKNNAEEMKNLVNFLQSHKKLIKALKKNIESIQNKKHLIYKNKSYNPLLLSCVKKMNMLK 83
Query: 384 ETLDLMFNKTEHIYSTL--SKSTYQEIRDQISIENIKWIGQNSIGLLSNIKESLSAKYKV 211
E +D + K ++++ L K+TY + + I ++K G + + + + KY+
Sbjct: 84 ENVDYI-QKNQNLFKELMNQKATYSFVNTKKKIISLKSQGHKKETSQNQNENNDNQKYQE 142
Query: 210 EVGQNPSVEEVNQNDEDSTHDEEANLRDYLSTNDD 106
++ +E + ND++ T+DEE + D TNDD
Sbjct: 143 VNDEDDVNDEEDTNDDEDTNDEE-DTNDDEDTNDD 176
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 32.7 bits (73), Expect = 1.0
Identities = 31/149 (20%), Positives = 70/149 (46%), Gaps = 12/149 (8%)
Frame = -2
Query: 666 ALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYY----EEFTDEMKKNSGDE 499
+++ +++ ++ + G+ +NS D ++TK++ + Y+ EE E KK G +
Sbjct: 148 SVERTKSVVNGSINTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQEELEMEAKKVEGFD 207
Query: 498 SKTESTVMQLAQNFSNKL--RVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKS 325
+ + + ++ S KL R +A+S V ++ET+ + T H+ K+
Sbjct: 208 MVQKPSNYERLESLSTKLCSRAYHQALSRV--KEAKQKSQETIS-QLHSTVHLIEFARKN 264
Query: 324 TY------QEIRDQISIENIKWIGQNSIG 256
+ Q +D++ + ++W + SIG
Sbjct: 265 MHSANQKIQGAQDKLYVSWVEW--KRSIG 291
>sp|P19269|AMY1_DEBOC Alpha-amylase 1 precursor (1,4-alpha-D-glucan glucanohydrolase 1)
Length = 512
Score = 32.3 bits (72), Expect = 1.3
Identities = 28/105 (26%), Positives = 48/105 (45%), Gaps = 5/105 (4%)
Frame = -2
Query: 552 HGYYEEFTDEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMS-SGNKETL 376
HGY+ + DE+ N G T + QLA ++ S+ M V YNH + +G+ ++
Sbjct: 116 HGYWMKNIDELNTNFG----TADELKQLASELHSR---SMLLMVDVVYNHYAWNGDGSSV 168
Query: 375 DLM----FNKTEHIYSTLSKSTYQEIRDQISIENIKWIGQNSIGL 253
D FN+ + + + Y DQ ++E+ W G + L
Sbjct: 169 DYSSFTPFNQQSYFHDYCLITNY---NDQTNVEDC-WEGDTEVSL 209
>sp|Q9TU19|NPHP1_CANFA Nephrocystin-1
Length = 565
Score = 32.3 bits (72), Expect = 1.3
Identities = 33/146 (22%), Positives = 56/146 (38%)
Frame = -2
Query: 540 EEFTDEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFN 361
EE +E ++ G+E ++E + +N S+ S ++ + +G DL F
Sbjct: 8 EEEEEESEEGGGEEEESEEEEEEKQENESHHQATSKEYIAVGDFTAQQAG-----DLTFK 62
Query: 360 KTEHIYSTLSKSTYQEIRDQISIENIKWIGQNSIGLLSNIKESLSAKYKVEVGQNPSVEE 181
K E + K WI +N+ G I + Y E GQ+ S EE
Sbjct: 63 KREILLIIEKKPDGW------------WIAKNAKGNKGLIPRTYVEPYNKEEGQDTSEEE 110
Query: 180 VNQNDEDSTHDEEANLRDYLSTNDDH 103
++ D + D+ A + D H
Sbjct: 111 DSEEDVE-VGDQTAGGEEVKQRTDSH 135
>sp|Q00496|BXE_CLOBO Botulinum neurotoxin type E precursor (BoNT/E) (Bontoxilysin E)
[Contains: Botulinum neurotoxin E light chain; Botulinum
neurotoxin E heavy chain]
Length = 1251
Score = 32.0 bits (71), Expect = 1.7
Identities = 37/160 (23%), Positives = 71/160 (44%), Gaps = 7/160 (4%)
Frame = -2
Query: 654 VNTILTFTLKNIEGLSN----VLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTE 487
+ TIL FT+K+ G S+ V+ +I + ++++W Y ++ M K + +K +
Sbjct: 642 IPTILVFTIKSFLGSSDNKNKVIKAINNALKERDEKWKEVYSFIVSNWMTKINTQFNKRK 701
Query: 486 STVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTYQEIR 307
+ Q QN V A+ T+ + +S E + + NK + K E+
Sbjct: 702 EQMYQALQN-------QVNAIKTIIESKYNSYTLEEKNELTNKYD------IKQIENELN 748
Query: 306 DQISI--ENI-KWIGQNSIGLLSNIKESLSAKYKVEVGQN 196
++SI NI +++ ++SI L I + E +N
Sbjct: 749 QKVSIAMNNIDRFLTESSISYLMKIINEVKINKLREYDEN 788
>sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2)
Length = 699
Score = 31.6 bits (70), Expect = 2.3
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = -2
Query: 708 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 529
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 528 DEMKKNSGDESKTESTVMQLAQNFSNK 448
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HSP81.2)
Length = 699
Score = 31.6 bits (70), Expect = 2.3
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = -2
Query: 708 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 529
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 528 DEMKKNSGDESKTESTVMQLAQNFSNK 448
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P30995|BXE_CLOBU Botulinum neurotoxin type E precursor (BoNT/E) (Bontoxilysin E)
[Contains: Botulinum neurotoxin E light chain; Botulinum
neurotoxin E heavy chain]
Length = 1251
Score = 31.6 bits (70), Expect = 2.3
Identities = 35/158 (22%), Positives = 71/158 (44%), Gaps = 5/158 (3%)
Frame = -2
Query: 654 VNTILTFTLKNIEGLSN----VLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTE 487
+ TIL FT+K+ G S+ V+ +I + ++++W Y ++ M K + +K +
Sbjct: 642 IPTILVFTIKSFLGSSDNKNKVIKAINNALKERDEKWKEVYSFIVSNWMTKINTQFNKRK 701
Query: 486 STVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTYQEIR 307
+ Q QN N L+ + + YN + K L ++ E I + L++
Sbjct: 702 EQMYQALQNQVNALKAIIESK----YNSYTLEEKNELTNKYD-IEQIENELNQKV----- 751
Query: 306 DQISIENI-KWIGQNSIGLLSNIKESLSAKYKVEVGQN 196
I++ NI +++ ++SI L + + E +N
Sbjct: 752 -SIAMNNIDRFLTESSISYLMKLINEVKINKLREYDEN 788
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,433,858
Number of Sequences: 369166
Number of extensions: 1555003
Number of successful extensions: 5210
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4946
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5202
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)