Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_P13
(846 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5A6N8|RFT1_CANAL Oligosaccharide translocation protein ... 74 7e-13
sp|P40913|RFT1_KLULA Oligosaccharide translocation protein ... 59 1e-08
sp|Q6FPE8|RFT1_CANGA Oligosaccharide translocation protein ... 53 9e-07
sp|P38206|RFT1_YEAST Oligosaccharide translocation protein ... 52 2e-06
sp|Q754Q7|RFT1_ASHGO Oligosaccharide translocation protein ... 46 1e-04
sp|O94302|RFT1_SCHPO Oligosaccharide translocation protein ... 45 3e-04
sp|P35194|YBA4_YEAST Hypothetical 287.5 kDa protein in PDR3... 33 0.76
sp|Q45132|YGRC_BACPF Hypothetical 8.8 kDa protein in grpA-g... 33 1.3
sp|P51829|ADCY7_MOUSE Adenylate cyclase type VII (ATP pyrop... 32 2.2
sp|Q25414|5HTR_LYMST 5-hydroxytryptamine receptor (5-HT rec... 32 2.2
>sp|Q5A6N8|RFT1_CANAL Oligosaccharide translocation protein RFT1
Length = 561
Score = 73.6 bits (179), Expect = 7e-13
Identities = 62/245 (25%), Positives = 113/245 (46%), Gaps = 16/245 (6%)
Frame = +2
Query: 113 QFIFRLITFVINLALIRYLEKDILGLVNVRLILLYSTVIFITREPFRKVCLGKNINKKN- 289
Q I +L+TFV+N +IRYL I+G V L + ST++F +RE R N N
Sbjct: 30 QIIAKLLTFVLNQLIIRYLSPSIIG-VTTYLEFICSTILFFSRESIRLSVQRVRNNSDNK 88
Query: 290 ---SRKLINLMWLILPFALFISISLTF---VWCEVLSFPIGYTEYKSVVMMYGLSAVIEL 451
++K++N L + FA I + + + + V+ YK V++++ S ++EL
Sbjct: 89 DYVAQKVVNFGILAIAFAFPIFMVIGYWQLNYSSVMDKLFVSPFYKPVIVLFVASVILEL 148
Query: 452 MSEPLWLISQMNLMVEFRTIIEALALSSRIILSTILFF---------HWPSLRLYAFGIA 604
+ EP++ + Q L R+ E A+ + I+S + + + + AF +A
Sbjct: 149 LVEPIYCLYQFQLDFGKRSKFEGSAIFVKCIVSVLSILLARQYFVDQKFEGVAICAFALA 208
Query: 605 QLFYSFAICFGYFIYFKIILNSNKKSSLISCDAITNYRDLFPDLFQFEIEKEQFQMIKGI 784
Q YS + Y + F+ +NK + + N R +F E++ ++KG
Sbjct: 209 QFSYSLTLFACYLMSFRFEFQNNKINYNLVKLKDENAR-------EFYFEQDTLTIVKGF 261
Query: 785 CFQLL 799
Q++
Sbjct: 262 FVQMI 266
>sp|P40913|RFT1_KLULA Oligosaccharide translocation protein RFT1
Length = 556
Score = 59.3 bits (142), Expect = 1e-08
Identities = 52/224 (23%), Positives = 101/224 (45%), Gaps = 34/224 (15%)
Frame = +2
Query: 113 QFIFRLITFVINLALIRYLEKDILGLVNVRLILLYSTVIFITREPFRKVCL--------G 268
Q + + + F++N L+RYL I G+ + L L STV+F +RE R L G
Sbjct: 22 QTLSKGVNFLLNTLLVRYLSPRIFGITSF-LEFLLSTVLFFSRESIRISTLRIKSTTDSG 80
Query: 269 KNINKKNSRKLINLMWLI----LPFALFISISLTFV---WCEVLSFPIGYTEYKSVVMMY 427
K ++ L LI +PF + + +S+ + + + S+ I +K+ + +
Sbjct: 81 KLEKVEDGEDTRTLQSLINFGYIPFVIGLPLSIILISWQYSNLNSYFIDLPYFKASIFLI 140
Query: 428 GLSAVIELMSEPLWLISQMNLMVEFRTIIEALALSSRIILSTILF--------------- 562
LS +IEL+SEP +L+ Q L R+ E+L ++ + + I+
Sbjct: 141 WLSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANFIIVVWFEKMVNGVGLELH 200
Query: 563 --FHWPSLRLYAFGIAQLFYSFAI--CFGYFIYFKIILNSNKKS 682
+ + ++AF + +L ++ + C+ Y Y ++ + S
Sbjct: 201 DDYKQEGIAIFAFAVGKLVHAMTLLACYSYNYYSEVYTTGERYS 244
>sp|Q6FPE8|RFT1_CANGA Oligosaccharide translocation protein RFT1
Length = 551
Score = 53.1 bits (126), Expect = 9e-07
Identities = 52/269 (19%), Positives = 126/269 (46%), Gaps = 32/269 (11%)
Frame = +2
Query: 92 SFTLAGFQFIFRLITFVINLALIRYLEKDILGLVNVRLILLYSTVIFITREPFR----KV 259
+F + G Q +++TF++N L+RYL I G + L + TV+F +RE R ++
Sbjct: 15 TFLMMG-QLFSKIVTFLLNNTLVRYLSPRIFG-ITAFLEFIVGTVLFFSREAIRLSTQRI 72
Query: 260 CLGKNI------NKKNSRKLINLMWLILPFALFISISLTFV---WCEVLSFPIGYTEYKS 412
G + ++ +S + + + ++P + I +S+ + + + + + ++
Sbjct: 73 ADGNDADNDHDHDRDDSALQVCVNFAMIPLFIGIPLSIGLIAWQYHNINGYFVTLPFFQW 132
Query: 413 VVMMYGLSAVIELMSEPLWLISQMNLMVEFRTIIEALALSSRIILSTILFFHW------- 571
V + ++EL++EPL++++Q L R+ E++A+++ +++ + + +
Sbjct: 133 SVFAIWVGIILELVNEPLFVLNQHFLNYGARSRYESIAVTANCLVNFTVVYSYEKKLILT 192
Query: 572 ----------PSLRLYAFGIAQLFYSFAICFGYFIYFKIILNSNK--KSSLISCDAITNY 715
+ + AF + +L Y+ + Y+ + + SNK K SL + N
Sbjct: 193 SYFDDSERFREGIAILAFALGKLAYAATLLMCYYYNYLMNFKSNKPFKLSLQKIKSKVNE 252
Query: 716 RDLFPDLFQFEIEKEQFQMIKGICFQLLV 802
+ + F+ +I + ++ +CF+ L+
Sbjct: 253 KQTY--YFRSDILEHFKKVYFQLCFKHLL 279
>sp|P38206|RFT1_YEAST Oligosaccharide translocation protein RFT1 (Requiring fifty-three
protein 1)
Length = 574
Score = 52.0 bits (123), Expect = 2e-06
Identities = 65/296 (21%), Positives = 129/296 (43%), Gaps = 47/296 (15%)
Frame = +2
Query: 50 KRIIQMESTI--MIRKSFTLAGF----QFIFRLITFVINLALIRYLEKDILGLVNVRLIL 211
K+ Q+ ST ++ +S T A F Q +L+TF++N LIR+L I G + L
Sbjct: 3 KKNSQLPSTSEQILERSTTGATFLMMGQLFTKLVTFILNNLLIRFLSPRIFG-ITAFLEF 61
Query: 212 LYSTVIFITREPFRKVCL-----GKNI--------------NKKNSRKLINLMWLILPFA 334
+ TV+F +R+ R L G I K + +N ++
Sbjct: 62 IQGTVLFFSRDAIRLSTLRISDSGNGIIDDDDEEEYQETHYKSKVLQTAVNFAYIPFWIG 121
Query: 335 LFISISL-TFVWCEVLSFPIGYTEYKSVVMMYGLSAVIELMSEPLWLISQMNLMVEFRTI 511
+SI L + + + ++ I ++ + + LS ++EL+SEP ++++Q L R+
Sbjct: 122 FPLSIGLIAWQYRNINAYFITLPFFRWSIFLIWLSIIVELLSEPFFIVNQFMLNYAARSR 181
Query: 512 IEALALSSRIILSTILFF-----HWP-----------SLRLYAFGIAQLFYSFAI--CFG 637
E++A+++ I++ I+ + +P + + AF + +L +S + C+
Sbjct: 182 FESIAVTTGCIVNFIVVYAVQQSRYPMGVVTSDIDKEGIAILAFALGKLAHSITLLACY- 240
Query: 638 YFIYFKIILNSNKKSSLISCDAITNYRDL---FPDLFQFEIEKEQFQMIKGICFQL 796
Y+ Y K S+ ++ +L +P + + + Q K + FQL
Sbjct: 241 YWDYLKNFKPKKLFSTRLTKIKTRENNELKKGYPKSTSYFFQNDILQHFKKVYFQL 296
>sp|Q754Q7|RFT1_ASHGO Oligosaccharide translocation protein RFT1
Length = 552
Score = 45.8 bits (107), Expect = 1e-04
Identities = 38/168 (22%), Positives = 81/168 (48%), Gaps = 10/168 (5%)
Frame = +2
Query: 92 SFTLAGFQFIFRLITFVINLALIRYLEKDILGLVNVRLILLYSTVIFITREPFRKVCL-- 265
+F + G Q +L+TFV++ L+R+L I G+ + L L STV+F +RE R L
Sbjct: 17 TFLMMG-QLFGKLVTFVLHNVLVRFLSPRIFGITSF-LDFLSSTVLFFSREAIRLATLRI 74
Query: 266 -----GKNINKKNSRKLINLMWLILPFALFISISLTFVWCEVLSFPIGYTE---YKSVVM 421
G + ++ + + +P + +++ + + +T+ + +
Sbjct: 75 KTGGDGGRGGEMSAELQTAVNFANIPMCIGAPLAVVLAVWQYSNLNSYFTQLPFFSWSIY 134
Query: 422 MYGLSAVIELMSEPLWLISQMNLMVEFRTIIEALALSSRIILSTILFF 565
+ LS + EL SEPL++++Q L R+ E A+++ +++ + +
Sbjct: 135 LVLLSILAELASEPLYVVNQFMLNYRKRSQFEGAAVAASCLVNFAVIY 182
>sp|O94302|RFT1_SCHPO Oligosaccharide translocation protein rft1
Length = 527
Score = 45.1 bits (105), Expect = 3e-04
Identities = 37/139 (26%), Positives = 62/139 (44%), Gaps = 15/139 (10%)
Frame = +2
Query: 110 FQFIFRLITFVINLALIRYLEKDILGLVNVRLILLYSTVIFITREPFR----------KV 259
FQ R++TF +N IR ++ +L ST++F++RE R +
Sbjct: 21 FQGSSRILTFFLNQLTIRLTSPSAYAFSSIHFEILQSTILFLSRESVRLAMQRIPSENAI 80
Query: 260 CLGKNINKKNSRKLINLMWLILPFALF-----ISISLTFVWCEVLSFPIGYTEYKSVVMM 424
+ S+KL + + LI +L I ISL S P + K+ + +
Sbjct: 81 ITSTSTESNKSKKLSDQLQLIKNTSLISVYIGIVISLLVSLFYFYSLP-NFPYSKTCIFI 139
Query: 425 YGLSAVIELMSEPLWLISQ 481
Y +S+ IEL+SEP + + Q
Sbjct: 140 YTVSSFIELLSEPYYEVLQ 158
>sp|P35194|YBA4_YEAST Hypothetical 287.5 kDa protein in PDR3-HTA2 intergenic region
Length = 2493
Score = 33.5 bits (75), Expect = 0.76
Identities = 23/80 (28%), Positives = 42/80 (52%), Gaps = 5/80 (6%)
Frame = -1
Query: 555 IVERIIRLDNANASMMVLNSTIKFI*-----LINHSGSDISSMTADKPYIITTDLYSVYP 391
+++ ++ + + VL+ T+ +I ++ HS D SS K II +++
Sbjct: 1673 VIKELMATLKRGSQIHVLSYTVHYILKSMHGVLKHSDLDTSSSMIVK--IIMENIFGFAG 1730
Query: 390 IGKDNTSHHTKVKEILINKA 331
KD+ ++HTKVKEI NK+
Sbjct: 1731 EEKDSENYHTKVKEIKSNKS 1750
>sp|Q45132|YGRC_BACPF Hypothetical 8.8 kDa protein in grpA-grpB intergenic region
Length = 77
Score = 32.7 bits (73), Expect = 1.3
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +2
Query: 83 IRKSFTLAGFQFIFRLITFVINLALIRYLEKDILGLVNVRLILL 214
I +SFTL F L T + LA+ RY K + LV ++L L+
Sbjct: 28 IAQSFTLLSIMISFLLFTSAVVLAMFRYFRKGKMQLVIIKLFLV 71
>sp|P51829|ADCY7_MOUSE Adenylate cyclase type VII (ATP pyrophosphate-lyase 7) (Adenylyl
cyclase 7)
Length = 1099
Score = 32.0 bits (71), Expect = 2.2
Identities = 24/73 (32%), Positives = 39/73 (53%), Gaps = 6/73 (8%)
Frame = +2
Query: 164 YLEKDILGLVNVRLILLYSTVIFITRE---PFRKVCLGKNINKKNSRK---LINLMWLIL 325
YLE+D+ +VN LIL Y+T+I ++R+ R CL K KK + + N+ L+L
Sbjct: 809 YLERDLKIMVNFYLILFYATLILLSRQIDYYCRLDCLWKKKFKKEHEEFETMENVNRLLL 868
Query: 326 PFALFISISLTFV 364
L ++ F+
Sbjct: 869 ENVLPAHVAAHFI 881
>sp|Q25414|5HTR_LYMST 5-hydroxytryptamine receptor (5-HT receptor) (Serotonin receptor)
Length = 509
Score = 32.0 bits (71), Expect = 2.2
Identities = 19/83 (22%), Positives = 43/83 (51%), Gaps = 11/83 (13%)
Frame = +2
Query: 275 INKKNSRKLINLMWLILPFALFISISLTFVWCE-----------VLSFPIGYTEYKSVVM 421
I ++++R+++ ++ ++ ALFISI F W + ++S GYT + +V
Sbjct: 203 IRRRSARRILLMIMVVWIVALFISIPPLFGWRDPNNDPDKTGTCIISQDKGYTIFSTVGA 262
Query: 422 MYGLSAVIELMSEPLWLISQMNL 490
Y V+ ++ +WL+++ +
Sbjct: 263 FYLPMLVMMIIYIRIWLVARSRI 285
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,502,122
Number of Sequences: 369166
Number of extensions: 1782069
Number of successful extensions: 5202
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5006
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5194
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8293644780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)