Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_O19-2
(720 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding... 49 1e-05
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 45 3e-04
sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding... 44 3e-04
sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-r... 41 0.003
sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546 32 1.3
sp|O60664|M6PBP_HUMAN Mannose-6-phosphate receptor binding ... 32 1.7
sp|Q5BLZ2|M6PBP_PIG Mannose-6-phosphate receptor binding pr... 32 2.2
sp|Q5RAV8|M6PBP_PONPY Mannose-6-phosphate receptor binding ... 30 5.0
sp|P12222|YCF1_TOBAC Hypothetical 226 kDa protein ycf1 (ORF... 30 6.5
sp|Q8NVH3|THID_STAAW Phosphomethylpyrimidine kinase (HMP-ph... 30 6.5
>sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding protein 1
Length = 431
Score = 49.3 bits (116), Expect = 1e-05
Identities = 60/261 (22%), Positives = 103/261 (39%), Gaps = 35/261 (13%)
Frame = +1
Query: 34 MTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAVR-- 207
+ +DR+ P+V S++ V + Y +K++N + AE T+ A +T +P AV+
Sbjct: 12 LEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETIQP-AVKLF 70
Query: 208 --QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLD 381
+ +DN + LD E++ P++ Y ++ E L+ PV K D
Sbjct: 71 EPSIQRLDNVMCKSLDILEQRIPLV-------YLPPEMMYWNTKEYMSDHLVRPVLKRAD 123
Query: 382 TV---------SKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD------EL 516
+V S + +A K V YL + T+ D+TD L
Sbjct: 124 SVKQIGNAVLESPLTTYAAERIDGAFTVGDKFV--DKYLVPIQTDQ--DQTDVKKLVSAL 179
Query: 517 LDKYLP----------------DNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQI 648
D + P D+N+ + + +R S K ++RL +R +
Sbjct: 180 NDNFSPIRQQLNRMFGHKSPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAEA 239
Query: 649 NALLKASLEKLHQIHVLIEEI 711
AL K S E +H + E I
Sbjct: 240 RALKKQSKEAIHVLFYAAELI 260
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 44.7 bits (104), Expect = 3e-04
Identities = 53/257 (20%), Positives = 94/257 (36%), Gaps = 23/257 (8%)
Frame = +1
Query: 10 SARIVISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTA 189
+A +V + + V RV + P+V+ + + V S Y K L + AEK +K V A
Sbjct: 2 AAAVVDPQQSVVMRVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAA 61
Query: 190 EPIAV-------RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKK 348
A+ Q+ + LDR E++ PI+ + + +
Sbjct: 62 MTSALPIIQKLEPQIAVANTYACKGLDRMEERLPILNQPTSEIVASARGAVTGAKDVVTT 121
Query: 349 TLMWPVTKTLDTVSKTSDFAXXXXXXXXXXXMKI-------VLPATYLTNVSTNYVLDKT 507
T+ TVS D + VL N + + K+
Sbjct: 122 TMAGAKDSVASTVSGVVDKTKGAVTGSVERTKSVVNGSINTVLGMVQFMNSGVDNAITKS 181
Query: 508 DELLDKYLP---DNNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALL 660
+ L+D+Y P + + + D+V K ++ LS K R Y +A ++
Sbjct: 182 EMLVDQYFPLTQEELEMEAKKVEGFDMVQKPSNYERLESLSTKLCSRAYHQALSRVKEAK 241
Query: 661 KASLEKLHQIHVLIEEI 711
+ S E + Q+H + I
Sbjct: 242 QKSQETISQLHSTVHLI 258
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
Length = 352
Score = 44.3 bits (103), Expect = 3e-04
Identities = 57/269 (21%), Positives = 97/269 (36%), Gaps = 44/269 (16%)
Frame = +1
Query: 13 ARIVISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAE 192
A+ ++ + ++R+ P+VN + + Y +K N + + LT AE + TA
Sbjct: 30 AKDLLPHLESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAA 89
Query: 193 PIAV---RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWP 363
P R + ++D ++ +D+ E K PII + D V+ + ++
Sbjct: 90 PFVTKLDRPIAYVDQTLVKGIDKLEVKAPIIKDTPQEIYNQAKSKVIDVVQPHLERVVKF 149
Query: 364 VTKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATY----LTNVSTNYVLDKTDELLDKYL 531
+ D A VL Y + V T L + LL+ Y
Sbjct: 150 KAAGQQKAASLKDLAWQKANE--------VLATQYGSLAVNGVDTTTAL--AERLLEYYF 199
Query: 532 P--------DNNQ-------------------CNEEPYSNADIVTKVKRLSGKTQQRLYK 630
P DN+ +E+P + V V RLS K +R+Y+
Sbjct: 200 PKCESDVEEDNDDKQNAVVQNGKSSENDMPVPASEDPVLHT--VQTVGRLSNKISRRVYR 257
Query: 631 RASEQINALLKASLE----------KLHQ 687
S QI + K ++ KLHQ
Sbjct: 258 NVSRQIKQVQKGNINDYLSSLIAALKLHQ 286
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 450
Score = 41.2 bits (95), Expect = 0.003
Identities = 53/246 (21%), Positives = 98/246 (39%), Gaps = 25/246 (10%)
Frame = +1
Query: 31 KMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-- 204
+++ V RV + P+V+ + + V S Y K L + AEK +K + A A+
Sbjct: 9 QLSVVTRVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGMKTITSVAVTSALPI 68
Query: 205 -----RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXY---HDSVEST----KK 348
Q+ + LDR E+K PI+ + D+V +T K
Sbjct: 69 IQKLEPQIAVANTYACKGLDRIEEKLPILNQPTNQVVANAKGAMTGAKDAVTTTVTGAKD 128
Query: 349 TLMWPVTKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNY--VLDKTDELLD 522
++ +T +D + + VL + + +S+ L K++ L+D
Sbjct: 129 SVASTITGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVMQLMSSGVENALTKSELLVD 188
Query: 523 KYLP---DNNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALLKASLE 675
+YLP D + + D+V K + LS K + R Y++A ++ + E
Sbjct: 189 QYLPLTKDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRSRAYQQALCRVEEAKRKGQE 248
Query: 676 KLHQIH 693
+ Q+H
Sbjct: 249 TISQLH 254
>sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546
Length = 62
Score = 32.3 bits (72), Expect = 1.3
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 580 VTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINK 717
+ VK L G++ ++L +E NA+ K + IHV+IEE+ K
Sbjct: 3 IINVKLLEGRSDEQLKNLVTEVTNAVEKTTGANREAIHVIIEEMQK 48
>sp|O60664|M6PBP_HUMAN Mannose-6-phosphate receptor binding protein 1 (Cargo selection
protein TIP47) (47 kDa mannose 6-phosphate
receptor-binding protein) (47 kDa MPR-binding protein)
(Placental protein 17) (PP17)
Length = 434
Score = 32.0 bits (71), Expect = 1.7
Identities = 42/188 (22%), Positives = 70/188 (37%), Gaps = 18/188 (9%)
Frame = +1
Query: 25 ISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLK----AVKDTAE 192
+ + + VDRV P+++ + + V + Y K S + AEK ++ A A+
Sbjct: 20 VQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQ 79
Query: 193 PIAVR---QLGFIDNAVLNQLDRFEKKCPI-------ILEXXXXXXXXXXXXYHDSVEST 342
PI + Q+ LD+ E+ PI +L + V S
Sbjct: 80 PILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSA 139
Query: 343 KKTLMWPVTKTLD----TVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD 510
K T+ +++ +D V D M L L+ V T VL K++
Sbjct: 140 KDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDT--VLGKSE 197
Query: 511 ELLDKYLP 534
E D +LP
Sbjct: 198 EWADNHLP 205
>sp|Q5BLZ2|M6PBP_PIG Mannose-6-phosphate receptor binding protein 1 (Cargo selection
protein TIP47)
Length = 439
Score = 31.6 bits (70), Expect = 2.2
Identities = 29/129 (22%), Positives = 51/129 (39%), Gaps = 7/129 (5%)
Frame = +1
Query: 25 ISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLK----AVKDTAE 192
+ + + VDRV P+++ + V + Y K S+ + AEK +K A A+
Sbjct: 21 VQQPSVVDRVAGMPLISSTCHMVSAAYTSTKESHPHVKTVCDVAEKGVKTLTAAAVSGAQ 80
Query: 193 PIAVR---QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWP 363
PI + QL LD+ E+ PI+ + V ++ +
Sbjct: 81 PILSKLEPQLTSASEYAHRGLDKLEENLPILQQPSEKVLADTKELVSSKVSEAREAVSNT 140
Query: 364 VTKTLDTVS 390
V+ DTV+
Sbjct: 141 VSSAKDTVA 149
>sp|Q5RAV8|M6PBP_PONPY Mannose-6-phosphate receptor binding protein 1
Length = 434
Score = 30.4 bits (67), Expect = 5.0
Identities = 42/188 (22%), Positives = 69/188 (36%), Gaps = 18/188 (9%)
Frame = +1
Query: 25 ISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLK----AVKDTAE 192
+ + + VDRV P+++ + + V + Y K S + AEK ++ A A+
Sbjct: 20 VQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTLTAAAVSGAQ 79
Query: 193 PIAVR---QLGFIDNAVLNQLDRFEKKCPI-------ILEXXXXXXXXXXXXYHDSVEST 342
PI + Q+ LD+ E+ PI +L + V S
Sbjct: 80 PILSKLEPQIASASEYAHRGLDKLEENLPILQQPTERVLADTKELVSSKVSGAQEMVSSA 139
Query: 343 KKTLMWPVTKTLD----TVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD 510
K T+ +++ +D V D M L L+ V T VL K+
Sbjct: 140 KDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDT--VLGKSG 197
Query: 511 ELLDKYLP 534
E D +LP
Sbjct: 198 EWADNHLP 205
>sp|P12222|YCF1_TOBAC Hypothetical 226 kDa protein ycf1 (ORF 1901)
Length = 1901
Score = 30.0 bits (66), Expect = 6.5
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +1
Query: 469 LTNVSTNYVLDKTDELLDKYLPDNNQCNEEPYSN 570
+ ++T L+ T++L+DKY+ NN+ N+E +N
Sbjct: 1166 IPRINTQLFLESTNKLIDKYI-SNNEINQEKINN 1198
>sp|Q8NVH3|THID_STAAW Phosphomethylpyrimidine kinase (HMP-phosphate kinase) (HMP-P
kinase)
sp|Q6G7L7|THID_STAAS Phosphomethylpyrimidine kinase (HMP-phosphate kinase) (HMP-P
kinase)
Length = 276
Score = 30.0 bits (66), Expect = 6.5
Identities = 15/59 (25%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = +1
Query: 22 VISKMTCVDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDN-TLTRAEKTLKAVKDTAEP 195
+I+ ++ + HY + ++S+ YV+ KS + ++DN T + TL + D P
Sbjct: 81 MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTP 139
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,866,157
Number of Sequences: 369166
Number of extensions: 1078840
Number of successful extensions: 3556
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3451
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3556
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)