Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_N16
(702 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O34153|GLPK_ENTCA Glycerol kinase (ATP:glycerol 3-phosph... 33 0.73
sp|Q99YI7|GLPK_STRP1 Glycerol kinase (ATP:glycerol 3-phosph... 33 0.96
sp|Q8E1T0|GLPK_STRA5 Glycerol kinase (ATP:glycerol 3-phosph... 33 0.96
sp|Q5XAJ9|GLPK_STRP6 Glycerol kinase (ATP:glycerol 3-phosph... 33 0.96
sp|Q8NZW9|GLPK_STRP8 Glycerol kinase (ATP:glycerol 3-phosph... 33 0.96
sp|Q8K665|GLPK_STRP3 Glycerol kinase (ATP:glycerol 3-phosph... 32 1.2
sp|P18157|GLPK_BACSU Glycerol kinase (ATP:glycerol 3-phosph... 32 2.1
sp|Q9WX53|GLPK_THEAQ Glycerol kinase (ATP:glycerol 3-phosph... 30 6.2
sp|P17898|CPT1_YEAST Diacylglycerol cholinephosphotransfera... 30 6.2
sp|Q81GZ2|GLPK_BACCR Glycerol kinase (ATP:glycerol 3-phosph... 30 8.1
>sp|O34153|GLPK_ENTCA Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 506
Score = 33.1 bits (74), Expect = 0.73
Identities = 20/73 (27%), Positives = 41/73 (56%), Gaps = 2/73 (2%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALV--STITSTVANQAVLKGIGVGDDKATLLAATVTWI 300
PQ FP+S ++ A ++W+++Q+++ + I S + +A+ GIG+ + + T TV W
Sbjct: 37 PQYFPKSGWVEHNANEIWNSVQSVIAGAFIESGIRPEAI-AGIGITNQRET----TVVWD 91
Query: 301 MKDGAGLIGRILF 339
G + I++
Sbjct: 92 KTTGQPIANAIVW 104
>sp|Q99YI7|GLPK_STRP1 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 508
Score = 32.7 bits (73), Expect = 0.96
Identities = 17/72 (23%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALVS-TITSTVANQAVLKGIGVGDDKATLLAATVTWIM 303
PQ FP + ++ A Q+W+++Q++++ + + ++ IG+ + + T TV W
Sbjct: 37 PQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRET----TVVWDK 92
Query: 304 KDGAGLIGRILF 339
K G + I++
Sbjct: 93 KTGVPIYNAIVW 104
>sp|Q8E1T0|GLPK_STRA5 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
sp|Q8E794|GLPK_STRA3 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 502
Score = 32.7 bits (73), Expect = 0.96
Identities = 17/72 (23%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALVS-TITSTVANQAVLKGIGVGDDKATLLAATVTWIM 303
PQ FP++ ++ A Q+W+++Q++++ + ++ IG+ + + T TV W
Sbjct: 38 PQIFPQAGWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRET----TVVWDK 93
Query: 304 KDGAGLIGRILF 339
K G + I++
Sbjct: 94 KTGLPIYNAIVW 105
>sp|Q5XAJ9|GLPK_STRP6 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 504
Score = 32.7 bits (73), Expect = 0.96
Identities = 17/72 (23%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALVS-TITSTVANQAVLKGIGVGDDKATLLAATVTWIM 303
PQ FP + ++ A Q+W+++Q++++ + + ++ IG+ + + T TV W
Sbjct: 37 PQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRET----TVVWDK 92
Query: 304 KDGAGLIGRILF 339
K G + I++
Sbjct: 93 KTGVPIYNAIVW 104
>sp|Q8NZW9|GLPK_STRP8 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 508
Score = 32.7 bits (73), Expect = 0.96
Identities = 17/72 (23%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALVS-TITSTVANQAVLKGIGVGDDKATLLAATVTWIM 303
PQ FP + ++ A Q+W+++Q++++ + + ++ IG+ + + T TV W
Sbjct: 37 PQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRET----TVVWDK 92
Query: 304 KDGAGLIGRILF 339
K G + I++
Sbjct: 93 KTGVPIYNAIVW 104
>sp|Q8K665|GLPK_STRP3 Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 508
Score = 32.3 bits (72), Expect = 1.2
Identities = 17/72 (23%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 127 PQGFPESVSDDYTAYQVWDTIQALVS-TITSTVANQAVLKGIGVGDDKATLLAATVTWIM 303
PQ FP + ++ A Q+W+++Q++++ + + ++ IG+ + + T TV W
Sbjct: 37 PQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRET----TVIWDK 92
Query: 304 KDGAGLIGRILF 339
K G + I++
Sbjct: 93 KTGVPIYNAIVW 104
>sp|P18157|GLPK_BACSU Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 496
Score = 31.6 bits (70), Expect = 2.1
Identities = 20/90 (22%), Positives = 47/90 (52%), Gaps = 1/90 (1%)
Frame = +1
Query: 73 KKNKIIFTFKRKLKEIFLPQGFPESVSDDYTAYQVWDTIQALV-STITSTVANQAVLKGI 249
K+ KI+ + +++ + F G+ E + A ++W ++ A++ S I+ + + + + GI
Sbjct: 22 KEGKIVHSAQKEFTQYFPHPGWVE-----HNANEIWGSVLAVIASVISESGISASQIAGI 76
Query: 250 GVGDDKATLLAATVTWIMKDGAGLIGRILF 339
G+ + + T TV W G+ + I++
Sbjct: 77 GITNQRET----TVVWDKDTGSPVYNAIVW 102
>sp|Q9WX53|GLPK_THEAQ Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 496
Score = 30.0 bits (66), Expect = 6.2
Identities = 16/71 (22%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
Frame = +1
Query: 130 QGFPESVSDDYTAYQVWDTIQALVSTITSTV-ANQAVLKGIGVGDDKATLLAATVTWIMK 306
Q FP+ ++ A ++W ++ A+++++ S + GIG+ + + T TV W+
Sbjct: 36 QYFPQPGWVEHNANEIWGSVLAVIASVLSEAQVKPEQVAGIGITNQRET----TVVWVKD 91
Query: 307 DGAGLIGRILF 339
G + I++
Sbjct: 92 TGNPIYNAIVW 102
>sp|P17898|CPT1_YEAST Diacylglycerol cholinephosphotransferase (SN-1,2-diacylglycerol
cholinephosphotransferase) (CHOPT)
Length = 386
Score = 30.0 bits (66), Expect = 6.2
Identities = 20/57 (35%), Positives = 27/57 (47%)
Frame = +1
Query: 400 ILNDISIFLDIFTAYLPQQMFIFIICLSSIGRSIVGVAGGATRAALTMHQSRRIIWP 570
+L + F IFT L Q FI + + SIG S+ V G A LTM + +P
Sbjct: 261 LLPFFAYFSSIFTLVLIQPSFISLALILSIGFSVAFVVGRMIIAHLTMQPFPMVNFP 317
>sp|Q81GZ2|GLPK_BACCR Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase)
(GK)
Length = 496
Score = 29.6 bits (65), Expect = 8.1
Identities = 19/76 (25%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
Frame = +1
Query: 73 KKNKIIFTFKRKLKEIFLPQGFPESVSDDYTAYQVWDTIQALVSTITSTV-ANQAVLKGI 249
KK +I+ + +++ + F G+ E + A ++W +I A+++T S + GI
Sbjct: 22 KKGEIVHSAQKEFTQHFPKPGWVE-----HNAQEIWGSILAVIATCLSEADVKPEQIAGI 76
Query: 250 GVGDDKATLLAATVTW 297
G+ + + T TV W
Sbjct: 77 GITNQRET----TVVW 88
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,588,906
Number of Sequences: 369166
Number of extensions: 1457804
Number of successful extensions: 3874
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3871
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6122130210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)