Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_N03
(896 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apri... 82 2e-15
sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich... 78 4e-14
sp|P12297|SUWA_DROME Suppressor of white apricot protein 76 1e-13
sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120) 51 4e-06
sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceos... 50 7e-06
sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein... 39 0.020
sp|Q8CH02|SF04_MOUSE Splicing factor 4 39 0.026
sp|Q7ZUS4|ST2S2_BRARE Cytosolic sulfotransferase 2 (SULT1 ST2) 37 0.075
sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1 37 0.098
sp|Q68FU8|SF04_RAT Splicing factor 4 37 0.098
>sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apricot protein homolog)
Length = 749
Score = 82.4 bits (202), Expect = 2e-15
Identities = 51/144 (35%), Positives = 78/144 (54%), Gaps = 1/144 (0%)
Frame = +3
Query: 399 DKYIDFCPFLGESPGNEDEYNSKRYDQSRYSAIHFDYDNNQEKNESQESKYEKEVEDNGT 578
++Y+D + E+E +R DQ +AI FDY + K +S+
Sbjct: 93 ERYLDMYKDIQREQEKEEE-EKRRNDQR--NAIGFDYGTGKVKARESDSE---------- 139
Query: 579 DAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNN-PKFVFL 755
D + P +K G+ P+ + IIE+TA F+ G Q+EI+IKAKQ NN +F FL
Sbjct: 140 DEPFEPPEGIKFPVGLELPSNMKLHHIIEKTASFIVANGTQMEIVIKAKQRNNAEQFGFL 199
Query: 756 NFDHKLYPFYRHVTQLIKTAQYIP 827
FDH+L PFY+++ +LI+ +YIP
Sbjct: 200 EFDHRLNPFYKYLQKLIREKKYIP 223
>sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich 8 (Suppressor of white
apricot protein homolog)
Length = 951
Score = 77.8 bits (190), Expect = 4e-14
Identities = 50/147 (34%), Positives = 80/147 (54%), Gaps = 15/147 (10%)
Frame = +3
Query: 426 LGESPGNEDEYNSKRYDQS-----RYSAIHF----DYDNNQEKNESQESKYEKE------ 560
L E E+EY KR+ ++ Y+A+ F DY + E E +E ++E
Sbjct: 123 LEEEARQEEEY--KRFSEALAEDGSYNAVGFTYGSDYYDPSEPTEEEEPSKQREKNEAEN 180
Query: 561 VEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNP 740
+E+N + ++ P L + + P T + AIIE+TA FV +G Q EI++KAKQ+ N
Sbjct: 181 LEEN--EEPFVAPLGLSVPSDVELPPTAKMHAIIERTASFVCRQGAQFEIMLKAKQAPNS 238
Query: 741 KFVFLNFDHKLYPFYRHVTQLIKTAQY 821
+F FL FDH L P+Y+ + + +K +Y
Sbjct: 239 QFDFLRFDHYLNPYYKFIQKAMKEGRY 265
>sp|P12297|SUWA_DROME Suppressor of white apricot protein
Length = 963
Score = 76.3 bits (186), Expect = 1e-13
Identities = 45/120 (37%), Positives = 68/120 (56%), Gaps = 6/120 (5%)
Frame = +3
Query: 486 YSAIHFDYDNNQEKNES--QESKYEKEVEDNGTDAA--YLCPPNLKLLP--GMITPATER 647
+S + F YD + S S ++ N ++ ++ P L + P M P T +
Sbjct: 171 FSQVGFQYDGQSAASTSIGGSSTATSQLSPNSEESELPFVLPYTLMMAPPLDMQLPETMK 230
Query: 648 EAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIKTAQYIP 827
+ AIIE+TA+F+A +G Q+EILIKAKQ+NN +F FL L P+YRH+ IK A++ P
Sbjct: 231 QHAIIEKTARFIATQGAQMEILIKAKQANNTQFDFLTQGGHLQPYYRHLLAAIKAAKFPP 290
>sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120)
Length = 791
Score = 51.2 bits (121), Expect = 4e-06
Identities = 30/79 (37%), Positives = 44/79 (55%)
Frame = +3
Query: 597 PPNLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLY 776
P K + G+I P E I+++TA FVA GP+ E I+ + NNPKF FLN + +
Sbjct: 33 PTPSKPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYH 91
Query: 777 PFYRHVTQLIKTAQYIPGK 833
+YRH K +++ GK
Sbjct: 92 AYYRH-----KVSEFKEGK 105
Score = 32.3 bits (72), Expect = 1.8
Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +3
Query: 657 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIK--TAQYIPG 830
+++ TA+FVA G Q + K+ N +F FL H L+ ++ T+L++ T IP
Sbjct: 166 VVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYF---TKLVEQYTKILIPP 222
Query: 831 KRHISHL 851
K S L
Sbjct: 223 KGLFSKL 229
>sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceosome associated protein 114)
(SAP 114) (SF3a120)
Length = 793
Score = 50.4 bits (119), Expect = 7e-06
Identities = 29/75 (38%), Positives = 43/75 (57%)
Frame = +3
Query: 609 KLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYR 788
K + G+I P E I+++TA FVA GP+ E I+ + NNPKF FLN + + +YR
Sbjct: 37 KPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYR 95
Query: 789 HVTQLIKTAQYIPGK 833
H K +++ GK
Sbjct: 96 H-----KVSEFKEGK 105
Score = 32.3 bits (72), Expect = 1.8
Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +3
Query: 657 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIK--TAQYIPG 830
+++ TA+FVA G Q + K+ N +F FL H L+ ++ T+L++ T IP
Sbjct: 166 VVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYF---TKLVEQYTKILIPP 222
Query: 831 KRHISHL 851
K S L
Sbjct: 223 KGLFSKL 229
>sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein RBP)
Length = 645
Score = 38.9 bits (89), Expect = 0.020
Identities = 30/102 (29%), Positives = 50/102 (49%), Gaps = 2/102 (1%)
Frame = +3
Query: 519 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 698
Q +E +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 162 QSPDEDEEEDYEQWLE-------------IKVSP----PEGAETRKVIEKLARFVAEGGP 204
Query: 699 QLEILIKAKQSNNPKFVFL-NFDHKLYPFYR-HVTQLIKTAQ 818
+LE + +NP F FL + + + + +YR V ++ K AQ
Sbjct: 205 ELEKVAMEDYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQ 246
Score = 37.4 bits (85), Expect = 0.057
Identities = 29/112 (25%), Positives = 50/112 (44%), Gaps = 7/112 (6%)
Frame = +3
Query: 504 DYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 665
DY +N +KN + Y K+V + +A + K+ P P E + E
Sbjct: 213 DYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQKSQAASQKVSP----PEDEEVKNLAE 268
Query: 666 QTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQ 818
+ A+F+AD GP++E + N F FL + + Y +YR + + A+
Sbjct: 269 KLARFIADGGPEVETIALQNNRENQAFSFLYEPNSQGYKYYRQKLEEFRKAK 320
>sp|Q8CH02|SF04_MOUSE Splicing factor 4
Length = 643
Score = 38.5 bits (88), Expect = 0.026
Identities = 31/103 (30%), Positives = 48/103 (46%), Gaps = 3/103 (2%)
Frame = +3
Query: 519 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 698
Q ++ +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 158 QSPDDDEEEDYEQWLE-------------IKVSP----PEGAETRRVIEKLARFVAEGGP 200
Query: 699 QLEILIKAKQSNNPKFVFL---NFDHKLYPFYRHVTQLIKTAQ 818
+LE + +NP F FL N LY + R V ++ K AQ
Sbjct: 201 ELEKVAMEDYKDNPAFTFLHDKNSREFLY-YRRKVAEIRKEAQ 242
Score = 35.0 bits (79), Expect = 0.28
Identities = 27/102 (26%), Positives = 46/102 (45%), Gaps = 7/102 (6%)
Frame = +3
Query: 504 DYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 665
DY +N +KN + Y ++V + +A K+ P P E + E
Sbjct: 209 DYKDNPAFTFLHDKNSREFLYYRRKVAEIRKEAQKPQAATQKVSP----PEDEEAKNLAE 264
Query: 666 QTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYR 788
+ A+F+AD GP++E + N F FL + + + Y +YR
Sbjct: 265 KLARFIADGGPEVETIALQNNRENQAFSFLYDPNSQGYRYYR 306
>sp|Q7ZUS4|ST2S2_BRARE Cytosolic sulfotransferase 2 (SULT1 ST2)
Length = 301
Score = 37.0 bits (84), Expect = 0.075
Identities = 34/122 (27%), Positives = 50/122 (40%), Gaps = 5/122 (4%)
Frame = +3
Query: 438 PGNEDEYNSKRYDQSRYSAIHFDYDNNQEKNESQESK-----YEKEVEDNGTDAAYLCPP 602
PG+ + + K D +D+ N K + S YE VED G + A LC
Sbjct: 159 PGDWNTFLQKFMDGRNVFGPWYDHVNGYWKKKQTYSNILYMFYEDMVEDTGREVARLCS- 217
Query: 603 NLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPF 782
G+ T ATERE KG Q +++ + K +N ++F K+ PF
Sbjct: 218 ----FLGLSTSATERER----------ITKGVQFDVMKQNKMTNYSTLPVMDF--KISPF 261
Query: 783 YR 788
R
Sbjct: 262 MR 263
>sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1
Length = 785
Score = 36.6 bits (83), Expect = 0.098
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +3
Query: 639 TEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFY 785
T E II+ TA+FVA G + +++NNP+F F+ H ++ F+
Sbjct: 187 TGEELDIIKLTAQFVARNGKSFLTGLSNRENNNPQFHFMKPTHSMFTFF 235
>sp|Q68FU8|SF04_RAT Splicing factor 4
Length = 644
Score = 36.6 bits (83), Expect = 0.098
Identities = 21/56 (37%), Positives = 35/56 (62%), Gaps = 2/56 (3%)
Frame = +3
Query: 657 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYR-HVTQLIKTAQ 818
+IE+ A+FVA+ GP+LE + +NP F FL + + + + +YR V ++ K AQ
Sbjct: 188 VIEKLARFVAEGGPELEKVAMEDYKDNPAFTFLHDKNSREFLYYRKKVAEIRKEAQ 243
Score = 35.8 bits (81), Expect = 0.17
Identities = 28/112 (25%), Positives = 50/112 (44%), Gaps = 7/112 (6%)
Frame = +3
Query: 504 DYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 665
DY +N +KN + Y K+V + +A K+ P P E + E
Sbjct: 210 DYKDNPAFTFLHDKNSREFLYYRKKVAEIRKEAQKPQAATQKVSP----PEDEEAKNLAE 265
Query: 666 QTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQ 818
+ A+F+AD GP++E + N F FL + + + Y +Y+ + + A+
Sbjct: 266 KLARFIADGGPEVETIALQNNRENQAFSFLYDPNSQGYRYYKQKLEEFRKAK 317
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,117,425
Number of Sequences: 369166
Number of extensions: 1652803
Number of successful extensions: 4772
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4765
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9030416440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)