Planarian EST Database


Dr_sW_011_L07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_L07
         (550 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9XSY9|OSTP_SHEEP  Osteopontin precursor (Bone sialoprote...    31   2.3  
sp|Q6P902|TXND2_MOUSE  Thioredoxin domain-containing protein...    30   3.0  
sp|P40021|YEM3_YEAST  Hypothetical 119.3 kDa protein in PIP1...    30   3.9  
sp|Q9AJ82|SCA4_RICMN  Antigenic heat-stable 120 kDa protein ...    30   3.9  
sp|Q57794|Y348_METJA  Hypothetical protein MJ0348                  30   5.1  
sp|Q9AJ79|SCA4_RICJA  Antigenic heat-stable 120 kDa protein ...    30   5.1  
sp|P57082|TBX4_HUMAN  T-box transcription factor TBX4 (T-box...    29   6.6  
sp|P04008|TALA_POVLY  Large T antigen                              29   8.6  
sp|Q52658|SCA4_RICCN  Antigenic heat-stable 120 kDa protein ...    29   8.6  
sp|P40457|MLP2_YEAST  Protein MLP2 (Myosin-like protein 2)         29   8.6  
>sp|Q9XSY9|OSTP_SHEEP Osteopontin precursor (Bone sialoprotein-1) (Secreted
           phosphoprotein 1) (SPP-1)
          Length = 278

 Score = 30.8 bits (68), Expect = 2.3
 Identities = 29/116 (25%), Positives = 46/116 (39%), Gaps = 6/116 (5%)
 Frame = +1

Query: 109 LKRPSKPIEKVNYHTEQKPNGNIMSY-EERSIRYAEERRRILGSQSEMIEEDII--EVLN 279
           LK  SK   + N  +      +  S+ E   +  A ++   L   SE    D +  E+  
Sbjct: 160 LKSKSKKFRRSNVESPDATEEDFTSHIESEEMHDAPKKTSQLTDHSEETNSDELPKELTP 219

Query: 280 KFNE---YSSRSEGARETKLpreSHKKYNSMGSDSLNNGDRRENPMILHRLSKADS 438
           K  E   +S+R E    +KL +E H   + +  D  +  D+R    I H L    S
Sbjct: 220 KAKEESKHSNRIESQENSKLSQEFHSLEDKLDLDHKSEEDKRLKIRISHELDSVSS 275
>sp|Q6P902|TXND2_MOUSE Thioredoxin domain-containing protein 2 (Spermatid-specific
           thioredoxin-1) (Sptrx-1) (Thioredoxin-4)
          Length = 515

 Score = 30.4 bits (67), Expect = 3.0
 Identities = 15/57 (26%), Positives = 28/57 (49%)
 Frame = +1

Query: 151 TEQKPNGNIMSYEERSIRYAEERRRILGSQSEMIEEDIIEVLNKFNEYSSRSEGARE 321
           T Q   GN +   E  ++ +E +   LG++ E +EE ++ V+    E+    + A E
Sbjct: 374 TIQSQEGNTIKSSEEDVQLSESKLLGLGAEIETLEEGLVRVIKDKEEFEEVLKDAGE 430
>sp|P40021|YEM3_YEAST Hypothetical 119.3 kDa protein in PIP1-GLN3 intergenic region
          Length = 1076

 Score = 30.0 bits (66), Expect = 3.9
 Identities = 27/99 (27%), Positives = 40/99 (40%), Gaps = 11/99 (11%)
 Frame = +1

Query: 139 VNYHTEQKPNGNIMSYEERSIRYAEERRRILGSQSEMIEEDIIEVLNKFNEYSSRSEGAR 318
           V Y+++ +P  N+   +     Y +E           IE+D  E  N+ NE    S    
Sbjct: 466 VKYYSKPEPAANVYIDD----LYEDEN---FDDDMNCIEDD--ESGNEGNEICGLSTRFE 516

Query: 319 ETKLpreSHKKYNSM-----------GSDSLNNGDRREN 402
           ET L     KK+N +           G D+ NNGD  E+
Sbjct: 517 ETSLKSNKVKKFNDLFNLSDDDEEEDGKDNSNNGDENES 555
>sp|Q9AJ82|SCA4_RICMN Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
            (Protein PS 120)
          Length = 1011

 Score = 30.0 bits (66), Expect = 3.9
 Identities = 31/109 (28%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
 Frame = +1

Query: 91   QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 255
            Q    IL   SK  +EKVN +     PNGN+ + EE+   SI+  +E  +  G++S   E
Sbjct: 841  QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTEE 900

Query: 256  EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 396
            +      N  ++ +   E   +T  KL +E  K+  ++ + S+   D R
Sbjct: 901  QQSFIKTNLIDDKTLSKEVRLQTIDKLLQE-QKRAEAIENPSVKTEDVR 948
>sp|Q57794|Y348_METJA Hypothetical protein MJ0348
          Length = 795

 Score = 29.6 bits (65), Expect = 5.1
 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
 Frame = +1

Query: 67  KLIIKTMSQP-KFQILKRPSKPIEKVNYHTEQKPNGNIMSYEERSIRYAEERRRI 228
           K I++ M QP K +     SK ++K+N       NG ++S    S+R++ E   I
Sbjct: 721 KAIVREMGQPRKIETFTILSKELQKINSSITVDYNGQLISMPVYSVRFSLENSNI 775
>sp|Q9AJ79|SCA4_RICJA Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
            (Protein PS 120) (rps120)
          Length = 1018

 Score = 29.6 bits (65), Expect = 5.1
 Identities = 31/109 (28%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
 Frame = +1

Query: 91   QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 255
            Q    IL   SK  +EKVN +     PNGN+ + EE+   SI+  +E  +  G++S   E
Sbjct: 842  QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTEE 901

Query: 256  EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 396
            +      N  ++ +   E   +T  KL +E  K+  ++ + S+   D R
Sbjct: 902  QQSFIKANLIDDKTLSKEIRLQTIDKLLQE-QKRAEAIENPSVKTEDVR 949
>sp|P57082|TBX4_HUMAN T-box transcription factor TBX4 (T-box protein 4)
          Length = 545

 Score = 29.3 bits (64), Expect = 6.6
 Identities = 15/58 (25%), Positives = 28/58 (48%)
 Frame = -1

Query: 523 IKPLLNXXXXXXYHQSRNTLRHQQITQPTNQPCSICAESWDSRVYHHYLENRSPSNYI 350
           + PLL       ++Q  N   H Q+ +P + P S      DS +++H L+ R  + ++
Sbjct: 290 VGPLLGTHQALQHYQHENGA-HSQLAEPQDLPLSTFPTQRDSSLFYHCLKRRDGTRHL 346
>sp|P04008|TALA_POVLY Large T antigen
          Length = 697

 Score = 28.9 bits (63), Expect = 8.6
 Identities = 15/56 (26%), Positives = 30/56 (53%), Gaps = 6/56 (10%)
 Frame = +1

Query: 25  FCLK*VTTSFLSYCKLIIK------TMSQPKFQILKRPSKPIEKVNYHTEQKPNGN 174
           FC+   T SF+ +CK ++K      T+S+P F++L+     +    +  E++ + N
Sbjct: 280 FCVSQCTFSFI-HCKAVVKPLELYKTLSKPPFKLLEENKPGVSMFEFQEEKEQSVN 334
>sp|Q52658|SCA4_RICCN Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
            (Protein PS 120) (RCA)
          Length = 1022

 Score = 28.9 bits (63), Expect = 8.6
 Identities = 30/109 (27%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
 Frame = +1

Query: 91   QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 255
            Q    IL   SK  +EKVN +     PNGN+ + EE+   +I+  +E  +  G++S   E
Sbjct: 846  QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEEAIKKVDELVKAFGTKSSTEE 905

Query: 256  EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 396
            +      N  ++ +   E   +T  KL +E  K+  ++ + S+   D R
Sbjct: 906  QQSFIKTNLIDDKTLSKEVRLQTIDKLLQE-QKRSEAIENPSVKTEDVR 953
>sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2)
          Length = 1679

 Score = 28.9 bits (63), Expect = 8.6
 Identities = 29/138 (21%), Positives = 61/138 (44%), Gaps = 25/138 (18%)
 Frame = +1

Query: 100 FQILKRPSKPIEKVNYHTEQKPNGNIMSYEERSIRYAE--ERRRILGSQSEM---IEEDI 264
           + +L++  K I K     E+    N++  E +S  Y+   + +++L   SE     +E++
Sbjct: 21  YPVLRKLYKKIAKFERSEEEVTKLNVLVDEIKSQYYSRISKLKQLLDESSEQKNTAKEEL 80

Query: 265 IEVLNKFNEYSSR---------------SEGARETKLpreSHKKYN-----SMGSDSLNN 384
             + ++ NE  SR                E  RE    +   ++Y+       G+DSLN+
Sbjct: 81  NGLKDQLNEERSRYRREIDALKKQLHVSHEAMREVNDEKRVKEEYDIWQSRDQGNDSLND 140

Query: 385 GDRRENPMILHRLSKADS 438
              +EN ++  +L + ++
Sbjct: 141 DLNKENKLLRRKLMEMEN 158
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,159,616
Number of Sequences: 369166
Number of extensions: 1041786
Number of successful extensions: 3104
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3019
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3102
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3833118120
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)