Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_K22
(599 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 63 5e-10
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 61 2e-09
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 55 2e-07
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 52 9e-07
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 52 9e-07
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 38 0.022
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 34 0.32
sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62) 33 0.72
sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase... 31 2.7
sp|P18745|ZO22_XENLA Oocyte zinc finger protein XLCOF22 31 2.7
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 63.2 bits (152), Expect = 5e-10
Identities = 42/158 (26%), Positives = 68/158 (43%), Gaps = 3/158 (1%)
Frame = +1
Query: 1 IVEFLNPSL-CSELGLCNSTIKQVIMRQPLVQINKSKKTSNCRLCCKFYNYL-QTISSKS 174
++E ++P L CS L LC+ T + V + + K C +C K YL + + S
Sbjct: 372 LLEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNS 427
Query: 175 SHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRK 351
+ +L L C+ + + +C + + V+ F C C K
Sbjct: 428 TKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHK 487
Query: 352 NPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
LG C WGP +WC + + A C AV+HC+ +VW
Sbjct: 488 PLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVW 523
Score = 37.7 bits (86), Expect = 0.022
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +1
Query: 358 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVWRQ 471
LG CT G WC + K A C GAVKHC VW +
Sbjct: 20 LGLKECTRGSAVWCQNVKTASDC--GAVKHCLQTVWNK 55
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 60.8 bits (146), Expect = 2e-09
Identities = 40/149 (26%), Positives = 61/149 (40%), Gaps = 2/149 (1%)
Frame = +1
Query: 25 LCSELGLCNSTIKQVIMRQPLVQINKSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGL 201
+CS L LC+S + V++ K C +C K YL + + S+ +L L
Sbjct: 382 VCSMLHLCSSRG----LPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAAL 437
Query: 202 VSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCT 378
C+ + R +C + + V+ F C C K LG C
Sbjct: 438 EKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGAEKCV 497
Query: 379 WGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
WGP +WC + + A C AV+HC +VW
Sbjct: 498 WGPSYWCQNMESAALC--NAVEHCRRHVW 524
Score = 36.6 bits (83), Expect = 0.050
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +1
Query: 358 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
LG CT G WC + K A C GAV+HC VW
Sbjct: 20 LGLRECTRGSAVWCQNVKTAADC--GAVQHCLQTVW 53
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 54.7 bits (130), Expect = 2e-07
Identities = 31/117 (26%), Positives = 50/117 (42%), Gaps = 2/117 (1%)
Frame = +1
Query: 121 CRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKS 297
C +C K YL+ + S+ +L L C+ + + +C + + ++
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 298 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
F C +C K LG C WGPG+WC + + A C AV HC+ +VW
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNSETAARC--NAVDHCKRHVW 553
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 52.4 bits (124), Expect = 9e-07
Identities = 41/181 (22%), Positives = 67/181 (37%), Gaps = 30/181 (16%)
Frame = +1
Query: 13 LNPS-LCSELGLCNS-------------TIKQVIMRQPL--------------VQINKSK 108
+NPS LC +GLC + I ++++P + K
Sbjct: 378 VNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQK 437
Query: 109 KTSNCRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAA 285
C +C K YL+ + S+ +L L C+ + + +C + ++
Sbjct: 438 NGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLE 497
Query: 286 FNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNV 462
F C +C K LG C WGP +WC + + A C AV HC+ +V
Sbjct: 498 ILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHV 555
Query: 463 W 465
W
Sbjct: 556 W 556
Score = 31.2 bits (69), Expect = 2.1
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +1
Query: 373 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
C+ G C K AV CG AVKHC+ VW
Sbjct: 25 CSGGSAVLCRDVKTAVDCG--AVKHCQQMVW 53
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 52.4 bits (124), Expect = 9e-07
Identities = 29/117 (24%), Positives = 50/117 (42%), Gaps = 2/117 (1%)
Frame = +1
Query: 121 CRLCCKFYNYLQTISSKSSHSNLLFGLVS-FCNQISAHERLECLKQFRNFNAVIAAFNKS 297
C +C Y K++ + + L+ C+ + +C++ + V+
Sbjct: 403 CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAE 462
Query: 298 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
F C +C +K LG++ C WGPG+WC + + A C AV HC +VW
Sbjct: 463 MMDPTFVCTKLGVCGAAKKPLLGEDACVWGPGYWCKNMETAAQC--NAVDHCRRHVW 517
Score = 40.8 bits (94), Expect = 0.003
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +1
Query: 358 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
L + C GP WC S + A CG AVKHC+ NVW
Sbjct: 21 LWQKDCAKGPEVWCQSLRTASQCG--AVKHCQQNVW 54
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 37.7 bits (86), Expect = 0.022
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +1
Query: 373 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
C GP FWC S +HAV C A+ HC VW
Sbjct: 31 CAQGPQFWCQSLEHAVQC--RALGHCLQEVW 59
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 33.9 bits (76), Expect = 0.32
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +1
Query: 373 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 465
C GP FWC S + A+ C A+ HC VW
Sbjct: 25 CARGPAFWCQSLEQALQC--RALGHCLQEVW 53
>sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62)
Length = 416
Score = 32.7 bits (73), Expect = 0.72
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
Frame = +1
Query: 43 LCNSTIKQVIMRQPLVQINKSKKTSNCRLCCKFYNYLQTISSKSSHSNLLFGLVSFCNQ- 219
+C T + + + Q ++ K S C ++ I K+S S FG++S CN
Sbjct: 215 ICMLTFEHEMEKAFAFQASQDKVCSIC---------MEVILEKASASERRFGILSNCNHT 265
Query: 220 --ISAHERLECLKQFRNFNAVIAAFNKSKTINEF 315
+S + C KQF N +I + + + I+EF
Sbjct: 266 YCLSCIRQWRCAKQFE--NPIIKSCPECRVISEF 297
>sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 2.7
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 537 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 427
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|P18745|ZO22_XENLA Oocyte zinc finger protein XLCOF22
Length = 435
Score = 30.8 bits (68), Expect = 2.7
Identities = 29/136 (21%), Positives = 58/136 (42%), Gaps = 2/136 (1%)
Frame = +1
Query: 19 PSLCSELGLCNSTIKQVIMRQPLVQINKSKKTSNCRLCCKFY--NYLQTISSKSSHSNLL 192
P CSE G S + + Q + + +K +C C K + + + + +H+
Sbjct: 293 PFSCSECGKSFSNQSCLRVHQ---RTHTGEKPYSCSECGKSFVTSSQLAVHRRRTHTGEK 349
Query: 193 FGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEFCEMTKMCSHQRKNPLGKNP 372
S C + +++ CL+ + + F+ S+ F +K+ SHQR+ G+ P
Sbjct: 350 PFSCSECGKCFSNQ--SCLRVHQRTHTGENLFSCSECGKSFVTSSKLASHQRQTHTGEKP 407
Query: 373 CTWGPGFWCVSRKHAV 420
+ C +RK ++
Sbjct: 408 VSCSECGKCFTRKRSL 423
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,607,710
Number of Sequences: 369166
Number of extensions: 1426553
Number of successful extensions: 3585
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3461
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3580
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)