Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_011_K03 (835 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P37879|SYK_CRIGR Lysyl-tRNA synthetase (Lysine--tRNA lig... 374 e-103 sp|Q99MN1|SYK_MOUSE Lysyl-tRNA synthetase (Lysine--tRNA lig... 374 e-103 sp|Q15046|SYK_HUMAN Lysyl-tRNA synthetase (Lysine--tRNA lig... 372 e-103 sp|Q22099|SYK_CAEEL Lysyl-tRNA synthetase (Lysine--tRNA lig... 372 e-103 sp|Q6F2U9|SYK_ORYSA Lysyl-tRNA synthetase (Lysine--tRNA lig... 355 7e-98 sp|Q9ZPI1|SYK_ARATH Lysyl-tRNA synthetase (Lysine--tRNA lig... 342 6e-94 sp|P15180|SYKC_YEAST Lysyl-tRNA synthetase, cytoplasmic (Ly... 317 2e-86 sp|Q43776|SYK_LYCES Lysyl-tRNA synthetase (Lysine--tRNA lig... 311 2e-84 sp|O67258|SYK_AQUAE Lysyl-tRNA synthetase (Lysine--tRNA lig... 236 6e-62 sp|Q9RXE1|SYK_DEIRA Lysyl-tRNA synthetase (Lysine--tRNA lig... 232 8e-61
>sp|P37879|SYK_CRIGR Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 597 Score = 374 bits (961), Expect = e-103 Identities = 179/259 (69%), Positives = 213/259 (82%), Gaps = 3/259 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +TE +LS +VK + G Y + Y+ DG Sbjct: 318 IGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDG 377 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 + YEIDF PP++RI M+ LE+AL +KLP + TEE+R ILD IC VEC P Sbjct: 378 PEGEAYEIDFTPPFRRISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPP 437 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 RTTARLLDKLVGE+LE CINPTFI +HP+IMSPLAKWHR+ GLTERFELFVMKKE+CN Sbjct: 438 RTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICN 497 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP+ QR+ FE+QAK KAAGD+EAMF+DE F A+E G+PPTAGWG+GIDR+TMF Sbjct: 498 AYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVTMF 557 Query: 726 LTDTDNIKEVLCFPAMKPE 782 LTD++NIKEVL FPAMKPE Sbjct: 558 LTDSNNIKEVLLFPAMKPE 576
>sp|Q99MN1|SYK_MOUSE Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 595 Score = 374 bits (960), Expect = e-103 Identities = 178/259 (68%), Positives = 213/259 (82%), Gaps = 3/259 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +TE +LS +VK + G Y + Y+ DG Sbjct: 316 IGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDG 375 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 + YE+DF PP++RI M+ LE+AL +KLP + TEE+R ILD IC VEC P Sbjct: 376 PEGQAYEVDFTPPFRRISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPP 435 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 RTTARLLDKLVGE+LE CI+PTFI +HP+IMSPLAKWHR+ GLTERFELFVMKKE+CN Sbjct: 436 RTTARLLDKLVGEFLEVTCISPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICN 495 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP+ QR+ FE+QAK KAAGD+EAMF+DE F A+E G+PPTAGWG+GIDRLTMF Sbjct: 496 AYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRLTMF 555 Query: 726 LTDTDNIKEVLCFPAMKPE 782 LTD++NIKEVL FPAMKPE Sbjct: 556 LTDSNNIKEVLLFPAMKPE 574
>sp|Q15046|SYK_HUMAN Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 597 Score = 372 bits (954), Expect = e-103 Identities = 177/259 (68%), Positives = 212/259 (81%), Gaps = 3/259 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +TE ++S +VK + G Y V Y+ DG Sbjct: 318 IGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDG 377 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 + Y++DF PP++RI+M+ LE+AL MKLP TEE+R ILD IC VEC P Sbjct: 378 PEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPP 437 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 RTTARLLDKLVGE+LE CINPTFI +HP+IMSPLAKWHR+ GLTERFELFVMKKE+CN Sbjct: 438 RTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICN 497 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP+ QR+ FE+QAK KAAGD+EAMF+DE F A+E G+PPTAGWG+GIDR+ MF Sbjct: 498 AYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMF 557 Query: 726 LTDTDNIKEVLCFPAMKPE 782 LTD++NIKEVL FPAMKPE Sbjct: 558 LTDSNNIKEVLLFPAMKPE 576
>sp|Q22099|SYK_CAEEL Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 572 Score = 372 bits (954), Expect = e-103 Identities = 173/265 (65%), Positives = 214/265 (80%), Gaps = 4/265 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 +G+ FRNE +DLTHNPEFTTCEFYMA+ADY D+I +TEDLLS++V + G Y ++Y+ +G Sbjct: 297 VGRLFRNEGIDLTHNPEFTTCEFYMAYADYEDVIQLTEDLLSSMVMSIKGTYKIEYHPNG 356 Query: 195 KT----YEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSA 362 YE+DF PP++R+ M GL E L LP P+ L TEE+R + DK+C DNNV+CSA Sbjct: 357 PNTEPVYEVDFTPPFKRVHMYDGLAEKLGATLPDPSTLHTEEAREVFDKLCRDNNVDCSA 416 Query: 363 PRTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELC 542 PRTTARLLDKLVGEYLE I+PTF++ HP+IMSPLAKWHR++PGLTERFELF + +E+ Sbjct: 417 PRTTARLLDKLVGEYLESTFISPTFLIGHPQIMSPLAKWHRSIPGLTERFELFAVTREIA 476 Query: 543 NAYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTM 722 NAYTELNDPI QR+RFEQQAKDK AGD+EA +DETF A+E G+PPT GWG+GIDRL+M Sbjct: 477 NAYTELNDPITQRQRFEQQAKDKDAGDDEAQMIDETFCNALEYGLPPTGGWGMGIDRLSM 536 Query: 723 FLTDTDNIKEVLCFPAMKPEFGIKK 797 LTD +NIKEVL FPAM+PE G +K Sbjct: 537 ILTDNNNIKEVLLFPAMRPEDGTEK 561
>sp|Q6F2U9|SYK_ORYSA Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 602 Score = 355 bits (912), Expect = 7e-98 Identities = 170/259 (65%), Positives = 207/259 (79%), Gaps = 3/259 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IGKQFRNE +DLTHNPEFTTCEFYMA+ADY DLI +TE +LS +VKEL G Y +KY+ +G Sbjct: 345 IGKQFRNEGIDLTHNPEFTTCEFYMAYADYNDLIELTETMLSGMVKELTGGYKIKYHANG 404 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 EIDF PP+++IDM+ LE K+ +P +L ++E+ L CA +V+C P Sbjct: 405 VEKPPIEIDFTPPFRKIDMIEELEAMAKLNIP--KDLSSDEANKYLIDACAKYDVKCPPP 462 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 +TT RLLDKLVG +LEE C+NPTFI+NHPEIMSPLAKWHR+ PGLTERFELFV K E+CN Sbjct: 463 QTTTRLLDKLVGHFLEETCVNPTFIINHPEIMSPLAKWHRSRPGLTERFELFVNKHEVCN 522 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP+VQR+RFE+Q KD+ +GD+EAM +DETF A+E G+PPT GWGLGIDRLTM Sbjct: 523 AYTELNDPVVQRQRFEEQLKDRQSGDDEAMALDETFCTALEYGLPPTGGWGLGIDRLTML 582 Query: 726 LTDTDNIKEVLCFPAMKPE 782 LTD+ NIKEVL FPAMKP+ Sbjct: 583 LTDSQNIKEVLLFPAMKPQ 601
>sp|Q9ZPI1|SYK_ARATH Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 626 Score = 342 bits (878), Expect = 6e-94 Identities = 167/258 (64%), Positives = 203/258 (78%), Gaps = 3/258 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IGKQFRNE +DLTHNPEFTTCEFYMAFADY DL+ +TE +LS +VKEL G Y +KYN +G Sbjct: 354 IGKQFRNEGIDLTHNPEFTTCEFYMAFADYNDLMEMTEVMLSGMVKELTGGYKIKYNANG 413 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 EIDF PP++RI+M+ LE+ K+ +P +L +EE+ L CA +V+C P Sbjct: 414 YDKDPIEIDFTPPFRRIEMIGELEKVAKLNIP--KDLASEEANKYLIDACARFDVKCPPP 471 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 +TTARLLDKLVGE+LE C+NPTFI+N PEIMSPLAKWHR+ GLTERFELF+ K ELCN Sbjct: 472 QTTARLLDKLVGEFLEPTCVNPTFIINQPEIMSPLAKWHRSKSGLTERFELFINKHELCN 531 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP+VQR+RF Q KD+ +GD+EAM +DETF A+E G+ PT GWGLGIDRL+M Sbjct: 532 AYTELNDPVVQRQRFADQLKDRQSGDDEAMALDETFCNALEYGLAPTGGWGLGIDRLSML 591 Query: 726 LTDTDNIKEVLCFPAMKP 779 LTD+ NIKEVL FPAM+P Sbjct: 592 LTDSLNIKEVLFFPAMRP 609
>sp|P15180|SYKC_YEAST Lysyl-tRNA synthetase, cytoplasmic (Lysine--tRNA ligase) (LysRS) Length = 591 Score = 317 bits (813), Expect = 2e-86 Identities = 152/260 (58%), Positives = 193/260 (74%), Gaps = 4/260 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKD- 191 IG+QFRNE +D+THNPEFTTCEFY A+AD +DL+ +TE + S +VKE+ G YI+KY+ D Sbjct: 321 IGRQFRNEGIDMTHNPEFTTCEFYQAYADVYDLMDMTELMFSEMVKEITGSYIIKYHPDP 380 Query: 192 ---GKTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSA 362 K E++F P++RI+M+ LE+ +K P +L T E+ L KI DN +EC Sbjct: 381 ADPAKELELNFSRPWKRINMIEELEKVFNVKFPSGDQLHTAETGEFLKKILVDNKLECPP 440 Query: 363 PRTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELC 542 P T AR+LDKLVGE LE+ CINPTFI HP++MSPLAK+ R+ PGL ERFE+FV KE+C Sbjct: 441 PLTNARMLDKLVGE-LEDTCINPTFIFGHPQMMSPLAKYSRDQPGLCERFEVFVATKEIC 499 Query: 543 NAYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTM 722 NAYTELNDP QR RFE+QA+ K GD+EA VDETF A+E G+PPT GWG GIDRL M Sbjct: 500 NAYTELNDPFDQRARFEEQARQKDQGDDEAQLVDETFCNALEYGLPPTGGWGCGIDRLAM 559 Query: 723 FLTDTDNIKEVLCFPAMKPE 782 FLTD++ I+EVL FP +KP+ Sbjct: 560 FLTDSNTIREVLLFPTLKPD 579
>sp|Q43776|SYK_LYCES Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 588 Score = 311 bits (796), Expect = 2e-84 Identities = 148/257 (57%), Positives = 195/257 (75%), Gaps = 3/257 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 +GK FRNE +DLTH+PEFT CE YMA+ADY DL+ +TE LLS +VK+L G Y ++Y+ +G Sbjct: 332 LGKHFRNEGMDLTHSPEFTMCELYMAYADYNDLMDLTEQLLSGMVKDLTGSYKIRYHANG 391 Query: 195 ---KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICADNNVECSAP 365 + EIDF PP+++IDM++ LE+ + +P +L +E + L +C +V+C P Sbjct: 392 LDNEPIEIDFTPPFRKIDMLSELEKVANISIP--RDLSSESANKHLVDVCEKFDVKCPPP 449 Query: 366 RTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKELCN 545 TT RLLDKLVG ++E CINPTFI+NHPEIMSPLAK R+ PGLTERF LFV ++ELC+ Sbjct: 450 HTTTRLLDKLVGHFIEVNCINPTFIINHPEIMSPLAKSRRSEPGLTERFNLFVNRRELCD 509 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 AYTELNDP QRERF +Q KD+ GD+EAM +DE+F+ A+E G+PPT G G+GIDRLTM Sbjct: 510 AYTELNDPTAQRERFAEQLKDRQLGDDEAMDLDESFITALEYGLPPTGGLGMGIDRLTML 569 Query: 726 LTDTDNIKEVLCFPAMK 776 LTD+ N+KEV+ FPAM+ Sbjct: 570 LTDSQNVKEVILFPAMR 586
>sp|O67258|SYK_AQUAE Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 597 Score = 236 bits (602), Expect = 6e-62 Identities = 125/261 (47%), Positives = 168/261 (64%), Gaps = 5/261 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IGK FRNESVD THNPEFT EFY A+ DY DLI TED+ L+++ G VKY + Sbjct: 344 IGKNFRNESVDRTHNPEFTMVEFYAAYWDYHDLIKFTEDMFVYLLEKTLGTLKVKYGE-- 401 Query: 195 KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILD-----KICADNNVECS 359 +E+DF PP++++ L+E + G ++ + D K+ + + Sbjct: 402 --WELDFSPPFKKVRYFDLLKE----------KTGKDKDFFLKDLEGLRKLAKELEIPDV 449 Query: 360 APRTTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVPGLTERFELFVMKKEL 539 T A+LLDK+ + EE I PTF+++ P+I+SPLAK HR P L ERFEL + + E+ Sbjct: 450 ERMTHAKLLDKVFEKVAEEDLIQPTFVIDFPKILSPLAKTHREDPDLVERFELIIARYEV 509 Query: 540 CNAYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLT 719 NAYTELNDP Q+ERF +Q K+K GDEEAM +DE F+ A+E GMPPTAG G+GIDRL Sbjct: 510 ANAYTELNDPFDQKERFLEQLKEKQMGDEEAMDMDEDFIRALEYGMPPTAGEGIGIDRLV 569 Query: 720 MFLTDTDNIKEVLCFPAMKPE 782 M L +TD+I+EV+ FP +KPE Sbjct: 570 MILANTDSIREVILFPQLKPE 590
>sp|Q9RXE1|SYK_DEIRA Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 525 Score = 232 bits (592), Expect = 8e-61 Identities = 129/261 (49%), Positives = 168/261 (64%), Gaps = 3/261 (1%) Frame = +3 Query: 15 IGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVTEDLLSNLVKELFGDYIVKYNKDG 194 IG+ +RNE +D THNPEFT E Y A+ADY D+ + EDLLS L KE+ G Y +Y G Sbjct: 263 IGRVYRNEGIDRTHNPEFTMLELYWAYADYSDIAKLVEDLLSGLAKEVHGSYQFEYQ--G 320 Query: 195 KTYEIDFKPPYQRIDMMTGLEEALKMKLPLPTELGTEESRLILDKICA--DNNVECSAPR 368 KT +DF PP+ R+D + GL E + G + L LD++ A D Sbjct: 321 KT--LDFTPPFARVDYLGGLREHVP---------GLDFDPLDLDRLRAFCDERFPQWKGV 369 Query: 369 TTARLLDKLVGEYLEEKCINPTFIMNHPEIMSPLAKWHRNVP-GLTERFELFVMKKELCN 545 + +LLDKL GE++E NPTF+M+HP ++SPLAK HR+ P +TERFE+F EL N Sbjct: 370 PSYKLLDKLFGEFVEPLLSNPTFVMDHPAVISPLAKKHRSRPEAVTERFEVFCSGFELAN 429 Query: 546 AYTELNDPIVQRERFEQQAKDKAAGDEEAMFVDETFLEAMELGMPPTAGWGLGIDRLTMF 725 A++ELND QRERFE Q +AAGD+EA DE FL A+E GMPP G G+GIDRL M Sbjct: 430 AFSELNDAFDQRERFEAQTARQAAGDDEAHPQDEDFLLALEYGMPPAGGLGIGIDRLAML 489 Query: 726 LTDTDNIKEVLCFPAMKPEFG 788 LTD+D+I++VL FP ++PE G Sbjct: 490 LTDSDSIRDVLLFPLLRPEGG 510
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 101,978,116 Number of Sequences: 369166 Number of extensions: 2137717 Number of successful extensions: 6539 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5965 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6344 length of database: 68,354,980 effective HSP length: 109 effective length of database: 48,218,865 effective search space used: 8100769320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)