Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_J12
(342 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P98158|LRP2_RAT Low-density lipoprotein receptor-related... 32 0.35
sp|Q9YHV4|FST_BRARE Follistatin precursor (FS) (Activin-bin... 32 0.59
sp|Q96N20|ZN75A_HUMAN Zinc finger protein 75A 31 1.0
sp|O62650|FST_HORSE Follistatin precursor (FS) (Activin-bin... 31 1.0
sp|O94588|CUF2_SCHPO Copper-binding regulatory protein cuf2... 30 2.3
sp|Q90844|FST_CHICK Follistatin precursor (FS) (Activin-bin... 30 2.3
sp|Q03376|BAR3_CHITE Balbiani ring protein 3 precursor 30 2.3
sp|P19883|FST_HUMAN Follistatin precursor (FS) (Activin-bin... 29 3.0
sp|P21674|FST_RAT Follistatin precursor (FS) (Activin-bindi... 29 3.0
sp|P10669|FST_PIG Follistatin precursor (FS) (Activin-bindi... 29 3.0
>sp|P98158|LRP2_RAT Low-density lipoprotein receptor-related protein 2 precursor
(Megalin) (Glycoprotein 330) (gp330)
Length = 4660
Score = 32.3 bits (72), Expect = 0.35
Identities = 27/84 (32%), Positives = 35/84 (41%), Gaps = 16/84 (19%)
Frame = +2
Query: 35 CDSGYK-EIDRVCK-------GKDTVECGLK------FDRHKKDCTDKQDYFWKVCGPVY 172
CD+G+ E+ RVC D CG+ R +CTD F+ C P Y
Sbjct: 3084 CDNGHCIEMGRVCNHVDDCSDNSDEKGCGINECLDSSISRCDHNCTDTITSFYCSCLPGY 3143
Query: 173 KTYSDE--CASAKERKEELEEISQ 238
K SD+ C E KE + SQ
Sbjct: 3144 KLMSDKRSCVDIDECKESPQLCSQ 3167
>sp|Q9YHV4|FST_BRARE Follistatin precursor (FS) (Activin-binding protein)
Length = 322
Score = 31.6 bits (70), Expect = 0.59
Identities = 21/54 (38%), Positives = 25/54 (46%), Gaps = 10/54 (18%)
Frame = +2
Query: 80 DTVECG----LKFDRHKKD----CTDKQDYFWK--VCGPVYKTYSDECASAKER 211
D V+CG K +R K D + WK VCG KTY DECA K +
Sbjct: 99 DNVDCGPGKRCKMNRRSKPRCVCAPDCSNVTWKGPVCGSDGKTYRDECALLKSK 152
>sp|Q96N20|ZN75A_HUMAN Zinc finger protein 75A
Length = 296
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 107 DRHKKDCTDKQDYFWKVCGPVYKTYSDECASAKERKEE 220
DRHKKDC ++ + + CG ++ SD + EE
Sbjct: 149 DRHKKDCAREKPFKCQECGKTFRVSSDLIKHQRIHTEE 186
>sp|O62650|FST_HORSE Follistatin precursor (FS) (Activin-binding protein)
Length = 344
Score = 30.8 bits (68), Expect = 1.0
Identities = 23/61 (37%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = +2
Query: 80 DTVECG--LKFDRHKKD------CTDKQDYFWK--VCGPVYKTYSDECASAKERKEELEE 229
D V+CG K +KK+ D + WK VCG KTY +ECA K R +E E
Sbjct: 96 DNVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPE 155
Query: 230 I 232
+
Sbjct: 156 L 156
>sp|O94588|CUF2_SCHPO Copper-binding regulatory protein cuf2 (Copper homeostasis protein)
Length = 177
Score = 29.6 bits (65), Expect = 2.3
Identities = 16/70 (22%), Positives = 30/70 (42%), Gaps = 12/70 (17%)
Frame = -2
Query: 182 CKSCKLVHKLSRNSLVCQCNLSYVYRISNHIQQ------------CLFLYKHDRFLYNQN 39
CK C+ + + +++L C C+L +N Q+ LFL+ D+ + N +
Sbjct: 42 CKKCRAIKQQLKSNLKCVCHLQPFLPFANEYQELLNFTQKNPILASLFLFSTDKDIMNSS 101
Query: 38 RNTHRFLYIY 9
N Y +
Sbjct: 102 LNPASQAYTF 111
>sp|Q90844|FST_CHICK Follistatin precursor (FS) (Activin-binding protein)
Length = 343
Score = 29.6 bits (65), Expect = 2.3
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = +2
Query: 80 DTVECG--LKFDRHKKD------CTDKQDYFWK--VCGPVYKTYSDECASAKERKEELEE 229
+ V+CG K +KK+ D + WK VCG KTY +ECA K R +E E
Sbjct: 95 ENVDCGPGKKCKMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPE 154
Query: 230 I 232
+
Sbjct: 155 L 155
>sp|Q03376|BAR3_CHITE Balbiani ring protein 3 precursor
Length = 1700
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/58 (32%), Positives = 21/58 (36%), Gaps = 16/58 (27%)
Frame = +2
Query: 35 CDSGYKEIDRVCK----------------GKDTVECGLKFDRHKKDCTDKQDYFWKVC 160
CD G K DRVC +T CG D K C +Q Y WK C
Sbjct: 625 CDGGQKWNDRVCSCGCPVPRPDCTNGQIYNINTCACGCGID--KPSCPKQQIYNWKTC 680
>sp|P19883|FST_HUMAN Follistatin precursor (FS) (Activin-binding protein)
Length = 344
Score = 29.3 bits (64), Expect = 3.0
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = +2
Query: 80 DTVECG--LKFDRHKKD------CTDKQDYFWK--VCGPVYKTYSDECASAKERKEELEE 229
+ V+CG K +KK+ D + WK VCG KTY +ECA K R +E E
Sbjct: 96 ENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPE 155
Query: 230 I 232
+
Sbjct: 156 L 156
>sp|P21674|FST_RAT Follistatin precursor (FS) (Activin-binding protein)
Length = 344
Score = 29.3 bits (64), Expect = 3.0
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = +2
Query: 80 DTVECG--LKFDRHKKD------CTDKQDYFWK--VCGPVYKTYSDECASAKERKEELEE 229
+ V+CG K +KK+ D + WK VCG KTY +ECA K R +E E
Sbjct: 96 ENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPE 155
Query: 230 I 232
+
Sbjct: 156 L 156
>sp|P10669|FST_PIG Follistatin precursor (FS) (Activin-binding protein)
Length = 344
Score = 29.3 bits (64), Expect = 3.0
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = +2
Query: 80 DTVECG--LKFDRHKKD------CTDKQDYFWK--VCGPVYKTYSDECASAKERKEELEE 229
+ V+CG K +KK+ D + WK VCG KTY +ECA K R +E E
Sbjct: 96 ENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPE 155
Query: 230 I 232
+
Sbjct: 156 L 156
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,320,946
Number of Sequences: 369166
Number of extensions: 549665
Number of successful extensions: 2000
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1999
length of database: 68,354,980
effective HSP length: 81
effective length of database: 53,391,445
effective search space used: 1708526240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)