Planarian EST Database


Dr_sW_011_J11

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_J11
         (720 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P57128|DNAA_BUCAI  Chromosomal replication initiator prot...    33   0.77 
sp|P47288|POTA_MYCGE  Spermidine/putrescine import ATP-bindi...    33   1.0  
sp|Q9JLN6|ADA28_MOUSE  ADAM 28 precursor (A disintegrin and ...    32   1.3  
sp|Q9UKQ2|ADA28_HUMAN  ADAM 28 precursor (A disintegrin and ...    32   1.7  
sp|Q9XSL6|ADA28_MACFA  ADAM 28 precursor (A disintegrin and ...    32   2.2  
sp|P57162|CYSE_BUCAI  Serine acetyltransferase (SAT)               31   3.8  
sp|P23394|PRP28_YEAST  Pre-mRNA splicing factor RNA helicase...    30   6.5  
sp|Q85197|POLG_PVMA  Genome polyprotein [Contains: P1 protei...    30   8.5  
sp|P12753|RAD50_YEAST  DNA repair protein RAD50 (153 kDa pro...    30   8.5  
sp|Q9NVH0|CN114_HUMAN  Protein C14orf114                           30   8.5  
>sp|P57128|DNAA_BUCAI Chromosomal replication initiator protein dnaA
          Length = 454

 Score = 33.1 bits (74), Expect = 0.77
 Identities = 23/76 (30%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
 Frame = +2

Query: 422 KMTDTLDSMTKTVKKARLNKDTKEMETPNRKDIDKIWERLINGKDDIL--YSKNNSPIIN 595
           ++ +T  SM     KA+LN +  E+  PN+  ++ + ++ +N    IL  Y   NSP+I 
Sbjct: 16  ELPNTEFSMWIRSLKAKLNNNILEIYAPNKFVLEWVKDKYLNHLKKILQDYCGTNSPLIK 75

Query: 596 ----PFYKETVTKRNL 631
                 YKE   K+N+
Sbjct: 76  FEIYQIYKENKLKKNI 91
>sp|P47288|POTA_MYCGE Spermidine/putrescine import ATP-binding protein potA
          Length = 559

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 25/92 (27%), Positives = 44/92 (47%), Gaps = 5/92 (5%)
 Frame = +2

Query: 416 KRKMTDTLDSMTKTVKKARLNKDTKEMET-----PNRKDIDKIWERLINGKDDILYSKNN 580
           ++K T+    +  T  K ++ K TKE++T      N+K IDK +++L+   +   Y  + 
Sbjct: 239 QKKKTELKADIKATKNKEQIQKLTKELQTLKQKYANKKAIDKEYDKLVVAYNKKDYWTSY 298

Query: 581 SPIINPFYKETVTKRNLFTRAIKTIRSKK*TD 676
                   KE   KR L  +  K  ++KK +D
Sbjct: 299 WETYTLQQKEAFEKRYLSRKLTKAEQNKKVSD 330
>sp|Q9JLN6|ADA28_MOUSE ADAM 28 precursor (A disintegrin and metalloproteinase domain 28)
           (Thymic epithelial cell-ADAM) (TECADAM)
          Length = 793

 Score = 32.3 bits (72), Expect = 1.3
 Identities = 14/45 (31%), Positives = 26/45 (57%)
 Frame = +2

Query: 479 KDTKEMETPNRKDIDKIWERLINGKDDILYSKNNSPIINPFYKET 613
           ++T+E E     + +  ++  +NGK  +LY K N+ ++ P Y ET
Sbjct: 44  RETQEPEPKETFETELRYKMTVNGKVAVLYLKKNNKLLAPDYSET 88
>sp|Q9UKQ2|ADA28_HUMAN ADAM 28 precursor (A disintegrin and metalloproteinase domain 28)
           (Metalloproteinase-like, disintegrin-like, and
           cysteine-rich protein-L) (MDC-L) (eMDC II) (ADAM23)
          Length = 775

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 15/45 (33%), Positives = 25/45 (55%)
 Frame = +2

Query: 479 KDTKEMETPNRKDIDKIWERLINGKDDILYSKNNSPIINPFYKET 613
           ++ KE E   + + +  ++  INGK  +LY K N  ++ P Y ET
Sbjct: 42  REAKEPEQQEQFETELKYKMTINGKIAVLYLKKNKNLLAPGYTET 86
>sp|Q9XSL6|ADA28_MACFA ADAM 28 precursor (A disintegrin and metalloproteinase domain 28)
           (eMDC II)
          Length = 776

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 14/45 (31%), Positives = 25/45 (55%)
 Frame = +2

Query: 479 KDTKEMETPNRKDIDKIWERLINGKDDILYSKNNSPIINPFYKET 613
           ++ KE E   + + +  ++  +NGK  +LY K N  ++ P Y ET
Sbjct: 43  REVKEPEQQEQFETELKYKMTVNGKIAVLYLKKNKNLLAPGYTET 87
>sp|P57162|CYSE_BUCAI Serine acetyltransferase (SAT)
          Length = 274

 Score = 30.8 bits (68), Expect = 3.8
 Identities = 13/50 (26%), Positives = 27/50 (54%)
 Frame = +2

Query: 518 IDKIWERLINGKDDILYSKNNSPIINPFYKETVTKRNLFTRAIKTIRSKK 667
           I +IW +++    D+ +     PI++ FY+ ++ +   FT ++  I S K
Sbjct: 6   ISRIWNKILY---DVSFLLKKEPILSDFYQSSILQHQSFTSSLSYILSNK 52
>sp|P23394|PRP28_YEAST Pre-mRNA splicing factor RNA helicase PRP28 (Helicase CA8)
          Length = 588

 Score = 30.0 bits (66), Expect = 6.5
 Identities = 15/46 (32%), Positives = 26/46 (56%)
 Frame = +2

Query: 461 KKARLNKDTKEMETPNRKDIDKIWERLINGKDDILYSKNNSPIINP 598
           K+ RL K+ +E  TP + D  K+  + +N +DD  +++ N    NP
Sbjct: 41  KQERL-KENEESLTPTQSDSAKVEIKKVNSRDDSFFNETNDKKRNP 85
>sp|Q85197|POLG_PVMA Genome polyprotein [Contains: P1 proteinase (N-terminal protein);
           Helper component proteinase (HC-pro); Protein P3; 6 kDa
           protein 1 (6K1); Cytoplasmic inclusion protein (CI); 6
           kDa protein 2 (6K2); Viral genome-linked protein (VPg);
           Nuclear inclusion protein A (NI-a) (NIa) (NIa-pro) (49
           kDa proteinase) (49 kDa-Pro); Nuclear inclusion protein
           B (NI-b) (NIb) (RNA-directed RNA polymerase); Coat
           protein (CP)]
          Length = 3059

 Score = 29.6 bits (65), Expect = 8.5
 Identities = 17/53 (32%), Positives = 24/53 (45%)
 Frame = +2

Query: 86  TFL*FMNLRSKASNSTEISIHPSIALNENFEDEYDEMDYILNCNEELLNECVK 244
           TF  F   R++  ++  IS HP+ A    F D Y     +LN N     E +K
Sbjct: 366 TFKEFQTQRAREISAVIISEHPNFACVSQFIDRYFSHQRVLNPNVNAYREILK 418
>sp|P12753|RAD50_YEAST DNA repair protein RAD50 (153 kDa protein)
          Length = 1312

 Score = 29.6 bits (65), Expect = 8.5
 Identities = 16/55 (29%), Positives = 27/55 (49%)
 Frame = +2

Query: 416  KRKMTDTLDSMTKTVKKARLNKDTKEMETPNRKDIDKIWERLINGKDDILYSKNN 580
            +RKM D+L  + KT+   ++ KD K  E     ++ K  E  ++  +  L  K N
Sbjct: 840  QRKMNDSLRELRKTISDLQMEKDEKVRENSRMINLIKEKELTVSEIESSLTQKQN 894
>sp|Q9NVH0|CN114_HUMAN Protein C14orf114
          Length = 496

 Score = 29.6 bits (65), Expect = 8.5
 Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
 Frame = +2

Query: 197 DYILNCNEELLNECVKRTIEIRK-----QYKKHVPRDILIKNAFEVL 322
           DY L   E L   C KR   IRK     +Y+KH P ++   N+ +VL
Sbjct: 285 DYYLMVKENLCVVCGKRDSYIRKNVIPHEYRKHFPIEMKDHNSHDVL 331
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,061,884
Number of Sequences: 369166
Number of extensions: 1102878
Number of successful extensions: 3117
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3056
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3116
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)